Pheromonal components in the feces of virgin queen honey bees, Apis mellifera L. Section: Cb. Apiculture & Social Insects Type: Poster display presentation Presenter: Finkelstein, Adam J. Contact Email: firstname.lastname@example.org Authors Finkelstein, A J, Department of Entomology, VPI& SU, Blacksburg, VA 24061 Fell, R D, Department of Entomology, VPI& SU, Blacksburg, VA 24061 Rausch, K M, Department of Entomology, VPI& SU, Blacksburg, VA 24061 Ruggio, D M, Department of Biochemistry, VPI& SU, Blacksburg, VA 24061 Jones, T H, Department of Chemistry, V.M.I., Lexington, VA 24450 Abstract: Virgin queen honey bees, Apis mellifera L., were reared, then allowed to emerge in cages on comb in small hives. Each day, for eight days, feces were sampled from different virgins. The feces were analyzed using capillary gas chromatography. Eight compounds were identified from their GC retention times and EI mass spectra. O-aminoacetophenone (O-AAP), previously described as a repellent, peaked between days three and five, and isoamylbutyrate (IAB), a previously undescribed ester in honey bees, was found at fairly constant levels over the eight days. Behavioral tests were conducted in observation hives to examine worker responses to the eight compounds; IAB triggered a more vigorous agitation and repellent response than O-AAP.