The lacZ database contains information on 405 mutations found in vivo in the transgenic lacZ gene. It has last been updated in January of 1998.
The database contains information on the mutagen, dose, organ, mutant fraction, base position, amino acid position, amino acid change, local DNA sequence, citation, and other items.
The database itself is in the dBASE format.
Routines have been developed for the analysis of single base substitutions, including programs to:
- (i) determine if two mutational spectra are different
- (ii) determine if mutations show a DNA strand bias
- (iii) determine the frequency of transitions and transversions
- (iv) display the number and kind of mutations observed at each base
- (v) perform nearest neighbor analysis
- (vi) display mutable amino acids in the lacZ protein.
The software runs only on IBM-compatible PCs running Microsoft Windows.
You can download the database and software.
- Cariello NF, Douglas GR and Soussi T. Databases and software for the analysis of mutations in the human p53 gene, the human hprt gene and the lacZ gene in transgenic rodents. Nucl. Acids Res., 24, 119-120, 1996.