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using bees to collect valuables from transgenic crops



The patent below is a novel way to use transgenic crops. Valuable products of
the transgenic crops will be collected by bees and the valuables recovered from
honey. In the event that all the bees have ben killed by eating trasgenic
insecticides in modified crops the next step will be to produce patented
synthetic bees!

EP0974667A1: Process to collect metabolites from modified nectar by insects

Creemers, Jantina, c/o CPRO-DLO;
Angenent, Gerrit Cornelis, CPRO-DLO;
Kater, Martin Maria, CPRO-DLO;

Assignee: CENTRUM VOOR PLANTENVEREDELINGS- EN REPRODUKTIEONDERZOEK (CPRO-DLO)


The invention relates to a recombinant double-stranded DNA molecule comprising
an expression cassette comprising the following constituents:i) a promoter
functional in nectaries of plants,ii) a DNA sequence encoding a protein which
is fused to the promoter,iii) a DNA sequence encoding a signal peptide that
targets the recombinant protein to nectar, which is translationally fused to
the DNA sequence encoding the recombinant protein, and optionallyiv) a signal
sequence functional in plants for the transcription termination and
polyadenylation of an RNA molecule. The invention further relates to a process
for producing a recombinant gene product from honey, comprising:i) producing a
transgenic plant by introducing in a plant cell a recombinant double-stranded
DNA molecule, regenerating plants from the transgenic cell, and selecting
modified plants exhibiting excretion of the recombinant gene product in
nectar,ii) allowing insects, preferably bees, to collect nectar from the
transgenic plants and to process the nectar into honey, andiii) isolating and
purifying the gene product from the honey.

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