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[SANET-MG] rice with human p450 gene resist most herbicides

Humanized rice is a bad idea. It may be the start of the soylent green
J. Agric. Food Chem., ASAP Article 10.1021/jf050064z S0021-8561(05)00064-6
Web Release Date: April 6, 2005

Copyright © 2005 American Chemical Society
Transgenic Rice Containing Human CYP2B6 Detoxifies Various Classes of

Sakiko Hirose,* Hiroyuki Kawahigashi, Kenjirou Ozawa, Noriaki Shiota,
Hideyuki Inui, Hideo Ohkawa, and Yasunobu Ohkawa

Plant Biotechnology Department, National Institute of Agrobiological
Sciences (NIAS), 2-1-2, Kannondai, Tsukuba, Ibaraki 305-8602, Japan, and
Research Center for Environmental Genomics, Kobe University, Rokkodaicho
1-1, Nada, Kobe 657-8501, Japan

Received for review January 12, 2005. Revised manuscript received
February 24, 2005. Accepted February 25, 2005. This work was supported
by the program for promotion of Basic Research Activities of Innovative
Biosciences of the Bio-oriented Technology Research Advancement
Institution (BRAIN).


The human gene for CYP2B6, a cytochrome P450 monooxygenase that
inactivates xenobiotic chemicals, was introduced into Oryza sativa cv.
Nipponbare by Agrobacterium-mediated transformation. At germination, R1
seeds of transgenic rice plants expressing CYP2B6 (CYP2B6 rice) showed a
high tolerance to 5 M metolachlor, a preemergence herbicide that is
degraded by CYP2B6. Thin-layer chromatography after culture with
14C-labeled metolachlor revealed that the amounts of residual
metolachlor decreased in plant tissues and the medium of CYP2B6 rice
faster than those of untransformed Nipponbare. CYP2B6 rice plants were
able to grow in the presence of 13 out of 17 herbicides: five
chloroacetamides and mefenacet, pyributicarb, amiprofos-methyl,
trifluralin, pendimethalin, norflurazon, and chlorotoluron. These
herbicides differ in their modes of action and chemical structures.
Transgenic rice expressing a xenobiotic-degrading human CYP2B6, which
has broad substrate specificity, should be good not only for developing
herbicide tolerant rice but also for reducing the environmental impact
of agrochemicals.

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