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[SANET-MG] problem genetic modification
I understand that the genetic modification described below was an
attempt to control old world Beatles..
Agric. Food Chem., 53 (23), 9023 -9030, 2005. 10.1021/jf050594v
Web Release Date: October 15, 2005
Transgenic Expression of Bean -Amylase Inhibitor in Peas Results in
Altered Structure and Immunogenicity
Vanessa E. Prescott, Peter M. Campbell, Andrew Moore, Joerg Mattes, Marc
E. Rothenberg, Paul S. Foster, T. J. V. Higgins, and Simon P. Hogan*
Division of Molecular Bioscience, The John Curtin School of Medical
Research, Australian National University, Canberra, ACT, Australia,
Division of Allergy and Immunology, Department of Pediatrics, Cincinnati
Children's Hospital Medical Center, University of Cincinnati College of
Medicine, Cincinnati, Ohio 45229, and Divisions of Entomology and Plant
Industry, Commonwealth Scientific and Industrial Research Organization,
Canberra, ACT, Australia
The development of modern gene technologies allows for the expression of
recombinant proteins in non-native hosts. Diversity in translational and
post-translational modification pathways between species could
potentially lead to discrete changes in the molecular architecture of
the expressed protein and subsequent cellular function and antigenicity.
Here, we show that transgenic expression of a plant protein (-amylase
inhibitor-1 from the common bean (Phaseolus vulgaris L. cv.
Tendergreen)) in a non-native host (transgenic pea (Pisum sativum L.))
led to the synthesis of a structurally modified form of this inhibitor.
Employing models of inflammation, we demonstrated in mice that
consumption of the modified AI and not the native form predisposed to
antigen-specific CD4+ Th2-type inflammation. Furthermore, consumption of
the modified AI concurrently with other heterogeneous proteins promoted
immunological cross priming, which then elicited specific
immunoreactivity of these proteins. Thus, transgenic expression of
non-native proteins in plants may lead to the synthesis of structural
variants possessing altered immunogenicity.
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