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[SANET-MG] swine fever virus fused potato virus x
The approach described below will likely encounter the problem of oral
tolerance. That will make the animal that regularly eats plants
recognize the swine virus as self. What will potato virus X do is
another problem, the glycoprotein may cause allergy or inflammation.
In plantaproduction of two peptides of the Classical Swine Fever Virus
(CSFV) E2 glycoprotein fused to the coat protein of potato virus X
Gianpiero Marconi , Emidio Albertini , Pierluigi Barone , Francesca De
Marchis , Chiara Lico , Carla Marusic , Domenico Rutili , Fabio
Veronesi and Andrea Porceddu
BMC Biotechnology 2006, 6:29 doi:10.1186/1472-6750-6-29
Published 22 June 2006
Classical Swine Fever (CSFV) is one of the most important viral
infectious diseases affecting wild boars and domestic pigs. The
etiological agent of the disease is the CSF virus, a single stranded RNA
virus belonging to the family Flaviviridae. All preventive measures in
domestic pigs have been focused in interrupting the chain of infection
and in avoiding the spread of CSFV within wild boars as well as
interrupting transmission from wild boars to domestic pigs. The use of
plant based vaccines against CSFV would be advantageous as plant organs
can be distributed without the need of particular treatments such as
refrigeration and therefore large areas, populated by wild animals,
could be easily covered.
We report the in planta production of peptides of the classical swine
fever (CSF) E2 glycoprotein fused to coat protein of potato virus X.
RT-PCR studies demonstrated that the peptide encoding sequences are
correctly retained in the PVX construct after three sequential passages
in Nicotiana benthamiana plants. Sequence analysis of RT-PCR products
confirmed that the epitope coding sequences are replicated with high
fidelity during PVX infections. Partially purified virions were able to
induce an immune response in rabbits.
Previous reports have demonstrated that E2 synthetic peptides can
efficiently induce an immunoprotective response in immunogenized
animals. In this work we have showed that E2 peptides can be expressed
in planta by using a modified PVX vector. These results are particularly
promising for designing strategies for disease containment in areas
inhabited by wild boars.
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