[Date Prev][Date Next][Thread Prev][Thread Next][Date Index][Thread Index]

[SANET-MG] swine fever virus fused potato virus x



The approach described below will likely encounter the problem of oral tolerance. That will make the animal that regularly eats plants recognize the swine virus as self. What will potato virus X do is another problem, the glycoprotein may cause allergy or inflammation. In plantaproduction of two peptides of the Classical Swine Fever Virus (CSFV) E2 glycoprotein fused to the coat protein of potato virus X Gianpiero Marconi , Emidio Albertini , Pierluigi Barone , Francesca De Marchis , Chiara Lico , Carla Marusic , Domenico Rutili , Fabio Veronesi and Andrea Porceddu

BMC Biotechnology 2006, 6:29     doi:10.1186/1472-6750-6-29

Published   22 June 2006

Abstract

Background

Classical Swine Fever (CSFV) is one of the most important viral infectious diseases affecting wild boars and domestic pigs. The etiological agent of the disease is the CSF virus, a single stranded RNA virus belonging to the family Flaviviridae. All preventive measures in domestic pigs have been focused in interrupting the chain of infection and in avoiding the spread of CSFV within wild boars as well as interrupting transmission from wild boars to domestic pigs. The use of plant based vaccines against CSFV would be advantageous as plant organs can be distributed without the need of particular treatments such as refrigeration and therefore large areas, populated by wild animals, could be easily covered.

Results

We report the in planta production of peptides of the classical swine fever (CSF) E2 glycoprotein fused to coat protein of potato virus X. RT-PCR studies demonstrated that the peptide encoding sequences are correctly retained in the PVX construct after three sequential passages in Nicotiana benthamiana plants. Sequence analysis of RT-PCR products confirmed that the epitope coding sequences are replicated with high fidelity during PVX infections. Partially purified virions were able to induce an immune response in rabbits.

Conclusions

Previous reports have demonstrated that E2 synthetic peptides can efficiently induce an immunoprotective response in immunogenized animals. In this work we have showed that E2 peptides can be expressed in planta by using a modified PVX vector. These results are particularly promising for designing strategies for disease containment in areas inhabited by wild boars.

********************************************************
To unsubscribe from SANET-MG:
1- Visit http://lists.sare.org/archives/sanet-mg.html to unsubscribe or;
2- Send a message to <listserv@sare.org> from the address subscribed to the list. Type "unsubscribe sanet-mg" in the body of the message.

Visit the SANET-MG archives at: http://lists.sare.org/archives/sanet-mg.html.
Questions? Visit http://www.sare.org/about/sanetFAQ.htm.
For more information on grants and other resources available through the SARE program, please visit http://www.sare.org.