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[SANET-MG] escape to the environment of transgenic Agrobacterium
Volume 26, Issue 11 , November 2006, Pages 3555-3561
Acta Ecologica Sinica
Survival and escape of Agrobacterium tumefaciens in triploid hybrid
lines of Chinese white poplar transformed with two insect-resistant genes
Minsheng Yanga, Dan Mia, D. Ewaldb, , , Ying Wangc, Haiyong Lianga and
aAgricultural University of Hebei, Baoding 071000, China
bInstitute for Forest Genetics and Forest Tree Breeding, BFH, D-15377
cInstitute of Tropical Biological Sciences, State Key Laboratory of
Tropical Crops Biotechnology, Haikou 571101, China
Received 10 January 2006; accepted 30 August 2006. Available online 19
Two partly modified insect-resistant genes (BtCryI Ac gene [Bt gene
toxin against Lepidopterean insects] and API gene [arrowhead proteinase
inhibitor]) were transferred to the triploid hybrid of Chinese white
poplar ((Populus tomentosa Carr. × Populus bolleana Louche) × Populus
tomentosa Carr.) mediated by A. tumefaciens. The survival of
Agrobacterium in transgenic plants was examined during the processes of
transplanting and subculturing on the nutrient medium. The results
suggested that 80% of the plants, which were obtained by repeated
selection on media added with 50 mg/L kanamycin and 300 mg/l
carbenicillin, showed positive reactions after examination using
molecular methods. The ELISA test indicated that the Bt toxoprotein was
expressed in seven of the transgenic sub-clones. Leaves, stems, and
roots of all the 28 transgenic plants were cultured on the YEB medium
added with 50 mg/L kanamycin, and it was found that Agrobacterium
survived in three sub-clones (33, 37, 5) and could have existed for 24
months in the bottle. These three transgenic sub-clones were
transplanted and cultivated for one month in the room, and then the
target Agrobacterium was found in rhizosphere of the sub-clone 33.
From conclusions:In the body of the transgenic plants could diffuse to the
soil after transplantation.
The results of the researches should be further tested in other kinds of
plants to confirm the possibilities of the gene
flow. Thus, if any plant transformation was performed with the help of
Agrobacteria, tests to detect the engineered bacteria
should be carried out before the transgenic plants would be released to
the environment to avoid the escape of genes.
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