This is a copy from a SANET posting. I like the fact that there is clear assessment of E. coli with temperature and time, butthey, as usual, forget to monitor the other orgnaisms, or the oxygen content.
Subj: [SANET-MG] E.coli and compost
Date: 1/7/2003 9:16:34 AM Eastern Standard Time
From: Rick Hopkins <rdhopkins_at_AMERICANPASTURAGE.COM>
To: SANET-MG_at_LISTS.IFAS.UFL.EDU
I found this article that may be of interest in the compost discussions and relevant undesired components.
FATE OF ESCHERICHIA COLI O157:H7 DURING COMPOSTING OF BOVINE MANURE IN A LABORATORY-SCALE BIOREACTOR
January 2003
Journal of Food Protection: Vol. 66, No. 1, pp. 2530
XIUPING JIANG,a JENNIE MORGAN,a and MICHAEL P. DOYLEa
www.foodprotection.org/QuickLinks.htm
aCenter for Food Safety, University of Georgia, 1109
Experiment Street, Griffin, Georgia 30223-1797, USA
ABSTRACT
Inactivation profiles of Escherichia coli O157:H7 in inoculated bovine manurebased compost ingredients were determined by composting these ingredients in a bioreactor under controlled conditions. A 15-liter bioreactor was constructed to determine the fate of E. coli O157:H7 and
changes in pH, moisture content, temperature, and aerobic mesophilic and thermophilic bacterial counts during composting. Fresh cow manure, wheat straw, cottonseed meal, and ammonium sulfate were combined to obtain a moisture content of ca. 60% and a carbon/nitrogen ratio of 29:1. The compost ingredients were held in the bioreactor at a constant external temperature of 21 or 50°C. Self-heating of the ingredients due to microbial activity occurred during composting, with stratified temperatures occurring within
the bioreactor. At an external temperature of 21°C, self-heating occurred for 0 to 3 days, depending on the location within the bioreactor. E. coli O157:H7 populations increased by 1 to 2 log10 CFU/g during the initial 24 h of composting and decreased by ca. 3.5 log10 CFU/g near the bottom of the
bioreactor and by ca. 2 log10 CFU/g near the middle and at the top during 36 days of composting. At an external temperature of 50°C, E. coli O157:H7 was inactivated rapidly (by ca. 4.9 log10 CFU/g at the top of the bioreactor, by 4.0 log10 CFU/g near the middle, and by 5.9 log10 CFU/g near the bottom)
within 24 h of composting. When inoculated at an initial level of ca. 107 CFU/g, E. coli O157:H7 survived for 7 days but not for 14 days at all three sampling locations, as indicated by either direct plating or enrichment culture. At the top of the bioreactor a relatively constant moisture content of 60% was maintained, whereas the moisture content near the bottom
decreased steadily to 37 to 45% over 14 days of composting. The pH of the composting mixture decreased to ca. 6 within 1 to 3 days and subsequently increased to 8 to 9. Results obtained in this study indicate that large populations (104 to 107 CFU/g) of E.. coli O157:H7 survived for 36 days during composting in a bioreactor at an external temperature of 21°C but were inactivated to undetectable levels after 7 to 14 days when the external temperature of the bioreactor was 50°C. Hence, manure contaminated with large populations (e.g., 107 CFU/g) of E. coli O157:H7 should be composted for more than 1 week, and preferably for 2 weeks, when held at a minimum
temperature of 50°C.
Rick Hopkins
President
American Pasturage, Inc.
P.O. Box 190
Marionville, MO 65705
(417) 258-2394
Elaine
President, Soil Foodweb Inc.
Corvallis, OR
Port Jefferson, NY
Lismore, NSW, Australia
Hilversum, The Netherlands
www.soilfoodweb.com
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Received on Tue Jan 07 2003 - 18:41:01 EST