Re: [compost_tea] Re: Cornelious vessels

From: Jack Hickman <jackhickman_at_bellsouth.net>
Date: Thu, 17 Mar 2005 18:54:33 -0600

This is all new for me.I was a millwright for a long,long time.I guess that=
 is why I like tinkering with things.
I used the oxygen water for my race horse(I don't race horses anymore)The p=
roblem I had,the oxygen would not stay in the water a significant time.
  ----- Original Message -----
  From: rhc1050
  To: compost_tea_at_yahoogroups.com
  Sent: Thursday, March 17, 2005 5:41 PM
  Subject: [compost_tea] Re: Cornelious vessels



  --- In compost_tea_at_yahoogroups.com, "Jack Hickman" <jackhickman_at_b...>
  wrote:
>
> I have a question.I had one of these vessels.These are used to
> dispense Coke and Pepsi at soda fountains.I used welder's oxygen
  which
> is pure oxygen.I set the vessel at 60 psi and pumped the oxygen in
  the
> vessel.Let set for 1 hour with,with pressure up.This gave me
  anywhere
> from 40 to 60 ppm dissolved oxygen in it.
> My question, would this make a better ACT?

  You are getting very close to the way one would grow large scale
  monocultures of bacteria or cells under laboratory conditions, where
  the culture is not nutrient limited and maximum cell density is
  desired. However, at high concentrations, oxygen can be toxic.
  Typically, we would fill our reactor with media, bubble air at a rate
  that was determined to be as fast as possible without shearing the
  cells, measure oxygen levels and set our meter such that the measured
  level was 100%. We would then add bacteria or cells and run at an
  oxygen level somewhat less than 100%, typically 70%. At some point,
  the culture density would be such that we could no longer maintain
  the desired level with air and we would switch to oxygen. We also
  monitored pH and adjusted for optimum and fed selected nutrients late
  in the culture cycle.

  To my mind, this is exactly how I would like to brew ACT, at least
  for experimental purposes (it wouldn't surprise me if Elaine already
  has). I'm working with an electrical engineer friend in NZ to see
  just how cheap we could build a set of automated controls. He's a
  pretty amazing guy, it wouldn't surprise me if he came up with
  something practical. The two flies in the ointment will be the cost
  of a decent oxygen probe and how the powers that be finally rule on
  feeding molasses (though I am making sum produce an organic medium
  without the use of added sugars).

  ron




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Received on Thu Mar 17 2005 - 22:04:49 EST

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