Subject: Tetracycline Tagging

From: [email-masked] (Craig John Mushens)
Date: 19 Mar 1996 18:26:07 GMT

I am looking for any information on techniques for tetracycline
injection of bull trout for the purpose of future age
reference. I am also curious as to whether it has any effects on
maturation or spawning behaviour.

Please respond to the newsgroup or e-mail me at 
[email-masked]

Thanks

Craig Mushens
Research Associate
University of Calgary

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From: [email-masked] (Stephen Walker)
Date: Thu, 21 Mar 1996 13:02:33

In article <[email-masked]> [email-masked] (Craig John Mushens) writes:
>From: [email-masked] (Craig John Mushens)
>Subject: Tetracycline Tagging
>Date: 19 Mar 1996 18:26:07 GMT

>Please respond to the newsgroup or e-mail me at 

Which calcified structures do you intend to mark? Monaghan (1993) induced 
marks in the otoliths of Summer Flounder using tetracycline and calcein at 25 
and 50 mg/kg. He found that tetracycline appeared detrimental to the health 
of the fish, while calcein did not. Injection was intramuscular, between the 
dorsal fin and lateral line.

I have used the same protocol to mark rainbow trout otoliths with calcein at 
approx. 30 mg/kg and found this effective. Mortality due to the treatment was 
negligible.

The reference is;

Monaghan, J.P. (1993) Comparison of calcein and tetracycline as chemical 
markers in Summer Flounder. Trans. Am. Fish. Soc. 122 No.2  pp298-301

Regards, 

Steve Walker

Steve [email-masked]| My opinion only.
>From  where I'm sitting, you lot don't all pass the Turing test.

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From: [email-masked] (Tor Haug)
Date: 25 Mar 1996 14:57:57 GMT

> Please respond to the newsgroup or e-mail me at 

Here are some references that may help you:

Choate, J. (1964). Use of tetracycline drugs to mark advanced fry and
fingerling brook trout (Salvelinus fontinalis). Trans. Am. Fish. Soc.,
93:309-311.

Hettler, W.F. (1984). Marking otoliths by immersion of marine fish larvae
in tetracycline. Trans. Am. Fish. Soc., 113:370-373.

Kobayashi, S., Yuki, R., Furui, T. & Kosugiyama, T. (1964). Calcification
in fish and shell-fish -I. Tetracycline labelling patterns on scale,
centrum and otolith in young goldfish. Bull.  Jap. Soc. Sci. Fish.,
30:6-13.

Koenings, J.P., Lipton, J. & McKay, P. (1986). Quantitative determination
of oxytetracycline uptake and release by juvenile sockeye salmon. Trans.
Am. Fish. Soc., 115:621-629.

McFarlane, G.A. & Beamish, R.J. (1987). Selection of dosages of
oxytetracycline for age validation studies. Can. J. Fish. Aquat. Sci.,
44:905-909.

Meunier, F.J. & Boivin, G. (1978). Action de la fluorescéine, de
l'alizarine, du bleu de calcéine et de diverse doses de tétracycline sur
la croissance de la truie et de la carpe. Ann. Biol. Anim. Bioch.
Biophys., 18:1293-1308.

Odense, P.H. & Logan, V.H. (1974). Marking atlantic salmon (Salmo salar)
with oxytetracycline. J. Fish. Res. Bd. Can., 31:348-350.

Scidmore, W.J. & Olson, D.E. (1969). Marking walleye fingerlings with
oxytetracycline antibiotic. Prog. Fish Cult., 31:213-216.

Szedlmayer, S.T. & Howe, J.C. (1995). An evaluation of six marking methods
for age-0 red drum, Sciaenops ocellatus. Fish. Bull., 93:191-195.

Wahl, D.H. & Stein, R.A. (1987). Application of liquid oxytetracycline in
formulated feeds to mark and treat tiger muskellunge (Northern Pike x
Muskellunge). Prog. Fish Cult., 49:312-314.

Weber, D. & Ridgeway, G.J. (1962). The disposition of tetracycline drugs
in bones and scales of fish and its possible use for marking. Prog. Fish
Cult., 24:150-155.

Weber, D. & Ridgeway, G.J. (1967). Marking pasific salmon with
tetracycline antibiotics. J. Fish. Res. Bd. Can., 22:849-865.

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-- 
Tor Haug ([email-masked])
The Norwegian College of Fishery Science
University of Tromsø
Norway