From FUNGI@UWYO.EDU Fri Dec 16 14:25:51 EST 1994
Article: 1200 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!howland.reston.ans.net!agate!biosci!UWYO.EDU!FUNGI
From: FUNGI@UWYO.EDU
Newsgroups: bionet.mycology
Subject: fungal geneticist position
Date: 16 Dec 1994 10:17:47 -0800
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FUNGAL GENETICIST, ASSISTANT PROFESSOR OF BOTANY
The Department of Botany at the University of Wyoming seeks 
applicants who work on the genetics of fungi for a 9-month tenure-
track position at the assistant professor level.  Applicants who can relate 
their work to organismal biology, and who use modern molecular 
techniques are especially encouraged to apply.  Preference will be given 
to applicants who complement existing strengths in mycology, 
systematics, evolutionary biology, physiology, and ecology.  The 
position begins in the fall, 1995, and the successful candidate will be 
expected to teach introductory genetics, participate in the general 
biology program and develop a graduate course in their specialty.  The 
successful candidate will be expected to teach introductory genetics, 
participate in the general biology program and develop a graduate 
course in area of specialization.  A vigorous, externally-funded, 
research program is expected and a commitment to graduate and 
undergraduate education is required.  A Ph.D. degree is required, and 
postdoctoral experience is desired.  Applicants should submit 
curriculum vita, transcripts, statement of teaching and research 
interests, and representative reprints; and should have three letters of 
reference sent by 15 February 1995 to Dr. Ron Hartman, Genetics 
Search Committee Chair, Department of Botany, University of 
Wyoming, Laramie, WY 82071-3165.  The University of Wyoming is 
an affirmative action-equal opportunity employer


From FUNGI@UWYO.EDU Fri Dec 16 14:25:52 EST 1994
Article: 1201 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!howland.reston.ans.net!agate!biosci!UWYO.EDU!FUNGI
From: FUNGI@UWYO.EDU
Newsgroups: bionet.mycology
Subject: post-doc position available
Date: 16 Dec 1994 10:22:33 -0800
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Position Title:  Postdoctoral Research Associate

Location:  Botany Department, University of Wyoming, Laramie, 
Wyoming.

Project:  National Science Foundation funded project entitled 
"Saprotrophic Responses by Ectomycorrhizal Fungi."  The 
mechanisms of ectomycorrhizal fungi that may allow greater 
competitivity with saprotrophic fungi will be examined.  This will be 
accomplished by conducting physiological and molecular experiments 
in the laboratory using specially designed root-microcosms that allow 
growth and manipulation of a portion of the extramatrical hyphae 
separate from the roots and rooting substrate while still in symbiosis 
with the host.  Some field work may also be required.

Qualifications:  A Ph.D. in mycology or closely related discipline, with 
research emphasis on fungi (especially ectomycorrhizal fungi and 
basidiomycetes) and training in growth, manipulation and 
experimentation with fungi, experience in fungal 
physiology and molecular techniques is required.  Knowledge of field 
techniques would be useful.  Must have ability to work independently 
and as a member of a team.

Salary:  Commensurate with experience.  Position is for up to 2 years 
(extensions may be possible), depending on experience and progress.  
Beginning date flexible but prefer soon.

Interested individuals should submit their curriculum vitae, a list of 
three references with addresses and phone numbers, and a letter 
describing capabilities and interests to:

Dr. Steven L. Miller
Botany Department
University of Wyoming
Laramie, Wyoming 82071
Phone:  (307) 766-2834
FAX:  (307) 766-2851
e-mail: FUNGI@UWYO.EDU


From craper@moose.uvm.edu Sat Dec 17 22:00:44 EST 1994
Article: 1202 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!news-feed-1.peachnet.edu!gatech!swrinde!pipex!warwick!news.dcs.warwick.ac.uk!hgmp.mrc.ac.uk!daresbury!not-for-mail
From: craper@moose.uvm.edu (Carlene Raper)
Newsgroups: bionet.mycology
Subject: Postdoctoral Position in Fungal Genetics Available
Date: 15 Dec 1994 15:59:30 -0000
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Original-To: mycology@dl.ac.uk

Seeking Postdoc

FUNGAL MOLECULAR GENETICIST P.I. SEEKING AMIABLE  POSTDOCTORAL ASSOCIATE
who yearns to fathom the mysteries of sex in a fungus with multiple mating
types.  Funding guaranteed for one year with possibility of continuation
for two more years. Start date as early as Jan. 15, 1995.  Qualified
candidates must have experience in genetics and molecular biology.

We have isolated and sequenced several putative pheromone and pheromone
receptor genes that reside in the two multispecific B mating-type loci of
the Basidiomycete Schizophyllum commune.  These genes encode molecules for
recognition of self versus many nonselves resulting in the initiation of a
defined pathway of sexual development leading to dikaryosis and mushroom
production.  Many mutants are available to study this process at the
molecular level.  The initial parts of this research will involve DNA
sequencing and sequence analyses, in vitro mutagenesis, and identification
of genes thought to be part of a signal transduction pathway, downstream of
the pheromone/receptor interactions.  Experiments designed to identify and
locate gene products in the cell are also planned.  

Our lab is located in a new building, fully supplied with the necessary and
latest  equipment to carry out this research.  The Department, which has
tripled in size over the past few years, includes 24 faculty members
representing a wide variety of interests in molecular genetics including
signal transduction, morphogenesis and cell-cycle control in yeast, 
mechanisms of DNA damage and repair, transcription complexes, and ribozyme
function.  It provides a cooperative learning environment for students and
postdocs at all levels. 

If interested, please send letter of application, curriculum vitae,  names
and addresses -- including e-mail address and/or phone numbers -- of three
persons who can evaluate you as a candidate. If preferred, reply using
above e-mail address. 
 
                Dr. Carlene A. Raper
                Dept. Microbiology and Molecular Genetics
                The L. P. Markey Center for Molecular Genetics
                Stafford Hall, Tel. (802) 656-1115; Fax. (802) 656-8749 
                University of Vermont, Burlington VT 05405

 




From stamets1@aol.com Sat Dec 17 22:00:45 EST 1994
Article: 1203 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!europa.eng.gtefsd.com!howland.reston.ans.net!swiss.ans.net!newstf01.news.aol.com!newsbf01.news.aol.com!not-for-mail
From: stamets1@aol.com (Stamets 1)
Newsgroups: bionet.mycology
Subject: Re: Fungi Perfect email?
Date: 17 Dec 1994 12:20:19 -0500
Organization: America Online, Inc. (1-800-827-6364)
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In article <mgalatz.1137979663B@198.7.0.1>, mgalatz@panix.com (Menachem
Galatz DC) writes:

Fungi Perfecti's  e-mail address is: Stamets1@aol.com. We do not send
catalogues via electronic media because, unfortunately, there are
unethical
individuals who have/will use my material  without permission. In essence,
it is already type-set! My lawyers at Preston, Gates &  Ellis underscore
that  Internet can be  a dangerous forum for writers and others who wish
 to protect their copyrighted materiasl. I am compelled to make this
 statement because 90% of everyone communicating with me ask
 for our catalogue & other material to be sent via the Net. And, given
some
hide behind the veil of anonymity, one can only wonder how their moral
compass is set. Hence,   I must continuously respond with "send me your
mailing address" and, frankly, am tiring of it. 

On another note, Merry Christmas folks.May the revolution continue...

paul stamets


From zorer@edvz.sbg.ac.at Sat Dec 17 22:00:46 EST 1994
Article: 1204 of bionet.mycology
Newsgroups: bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!howland.reston.ans.net!EU.net!Austria.EU.net!newsfeed.ACO.net!edvz.sbg.ac.at!DPFL03!zorer
From: zorer@edvz.sbg.ac.at (Roberto Zorer)
Subject: Third IAL Symposium: preliminary announcement
Message-ID: <zorer.5.2EF17042@edvz.sbg.ac.at>
Keywords: International Association for Lichenology, Lichen
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                                            Third IAL Symposium: preliminary announcement

                            (online WWW - Informations:  http://www.edvz.sbg.ac.at/pfl/ial3/prelimi.htm)

Third IAL Symposium - IAL3: preliminary announcement 
Progress and Problems in Lichenology in the Nineties

(Salzburg, Austria 1 - 7 September 1996)

Preliminary announcement

Arranged by the Intitute of Plant Physiology, University of Salzburg and the International Association for Lichenology

Organisation
I. Kärnefelt, President
R. Türk, Secretary

Local organizing Committee and Advisory Board
O. Breuss, G. Gärtner, J. Hafellner, H. Mayrhofer, E. Stocker-Wörgötter, J. Üblagger

General Information
The third international meeting arranged by the IAL and the Insitute of Plant Physiology of the University of Salzburg will be held from 1st to 7th September 1996 at the University of Salzburg, Faculty of Natural Sciences.

This symposium continues the series of international meetings on lichen biology which started at Berlin 1969. It is open to any person interested in lichenized fungi or working in fields such as morphology, physiology, ecology, chemistry and systematics o

Preliminary Announcement and Second Circular
If you are interested in receiving further information on the symposium please return the attached preliminary reply form before 31 st of January 1995. The second circular will be distributed during May 1995.

Language
The language at the Congress will be English.

Scientific Programme
The symposium will include oral contributions and poster sessions. It is planed to avoid simultaneous sessions. The preliminary scientific programme includes nine different sections:

A. Systematics
B. Morphology
C. Ecology
D. Chemistry
E. Changes of Environment and Conservation
F. Resynthesis and Coltivation
G. Lichens of High Mountains
H. Photobionts
I. Lichens in the Tropics

Scientific Sessions
An estimated 20 minutes, comprising a 15 minutes lecture and a 5 minutes discussion will be allowed for each lecture.

Contributed Papers
Contributed papers may be accepted either as lectures or as posters depending on the decision of the convenors.

Travel and Accomodations
Salzburg can be reached by train and by air. There is the possibility to arrive by air in Vienna (Austria) or in Munich (Germany) and take the regional train to Salzburg.
All the symposium partecipants can be offered hotel accomodation in modern student dormitories wich are used as hotel during summer time for a reasonable price. Camping accomodation is also available

Congress Fee
The Congress Fee will be estimated at 2.200.-- ATS (approximately equal to 220.-- $) for full members and 1.100.-- ATS for students.

Excursions
Four different excursions are offered before and after the Congress. Details will be presented in the second circular.

Correspondence
All correspondence and inquiries should be adressed to the scientific secretariat:

Dr. Roman Türk
University of Salzburg, Institute of Plant Physiology
Hellbrunnerstr. 34
A - 5020 Salzburg, Austria

Telephone Nat.: 0662 8044 5588; 0662 8044 5559
Telephone Int.: +43 662 8044 5588;  +43 662 8044 5559
Fax: +43 662 8044 5010
E-mail: tuerk@edvz.sbg.ac.at



From D.BRAYFORD@CABI.org Sat Dec 17 22:00:47 EST 1994
Article: 1205 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!howland.reston.ans.net!agate!biosci!CABI.org!D.BRAYFORD
From: D.BRAYFORD@CABI.org ("David Brayford ", IMI)
Newsgroups: bionet.mycology
Subject: re:  "ascomycotina" help
Date: 16 Dec 1994 03:17:45 -0800
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Sorry to sir things up, but the replies concerning classification of Xylaria 
hypoxylon
were slightly up the creek, or at least out of date.  The class"Ascomycetes" 
was got
rid of many moons ago and replaced by the subdivision "Ascomycotina".   More 
recently
the Ascomycotina have been got rid of too - and elevated to the rank of 
Phylum (= Division).
There are no classes, just orders and families.  So now we have:
Kingdom:  Fungi
Phylum:  Ascomycota
Order:  Xylariales
Family:  Xylariaceae
Genus:  Xylaria
Species:  hypoxylon

These changes are enshrined in the latest version of the International Code 
of Botanical
 Nomenclature and will be reflected in the forthcoming edition of The 
Dictionary of the
 Fungi.

Hope that's of some help.

Dave Brayford
International Mycological Institute
d.brayford@cabi.org


From biochem@clio.rz.uni-duesseldorf.de Sun Dec 18 09:36:42 EST 1994
Article: 1206 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!hearst.acc.Virginia.EDU!portal.gmu.edu!europa.eng.gtefsd.com!gatech!swrinde!pipex!uknet!daresbury!not-for-mail
From: "Weiss" <biochem@clio.rz.uni-duesseldorf.de>
Newsgroups: bionet.mycology
Subject: Fatty acid synthesis in fungi?
Date: 14 Dec 1994 17:07:31 -0000
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Hello molecular mycologists out there!

I've been told to find some information about fatty acid synthesis in 
filamentous fungi. My special interest is in the molecular biology of fatty 
acid synthetase complex.
Are there some enzymes cloned and sequenced? (I'm only aware of a 
Penicillium sequence) 
Are there mutants which are completely deficient in fatty acid synthesis? 
If so, how do they survive?
Does anyone know if the enzymes are sequenced in Neurospora?
And quite a lot more questions....

Who can point me to some literature or tell me who and where the experts 
are? Any help is greatly welcomed!

Thank you!    Christoph Kruell


Christoph Kruell
Institut fuer Biochemie
Universitaet Duesseldorf
Universitaetsstr. 1
40225 Duesseldorf
Germany

Phone: 49-211-311-5156
Fax:   49-211-311-3085
e-mail: biochem@clio.rz.uni-duesseldorf.de


From czhang@katk.helsinki.fi Sun Dec 18 09:36:43 EST 1994
Article: 1207 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!hearst.acc.Virginia.EDU!portal.gmu.edu!europa.eng.gtefsd.com!newsxfer.itd.umich.edu!gatech!swrinde!pipex!warwick!news.dcs.warwick.ac.uk!hgmp.mrc.ac.uk!daresbury!trane.uninett.no!sunic!news.funet.fi!news.csc.fi!news.helsinki.fi!valtuni5.pc.Helsinki.FI!czhang
From: czhang@katk.helsinki.fi (Zhang Chunmei            )
Newsgroups: bionet.mycology
Subject: zhang
Date: Wed, 14 Dec 1994 17:11:05 GMT
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cjlklö,,öjoj.


From moldgrower@aol.com Mon Dec 19 00:31:28 EST 1994
Article: 1208 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!rutgers!gatech!swiss.ans.net!newstf01.news.aol.com!newsbf01.news.aol.com!not-for-mail
From: moldgrower@aol.com (Moldgrower)
Newsgroups: bionet.mycology
Subject: Re: Manipulations with Filamentous Fungi
Date: 17 Dec 1994 15:35:13 -0500
Organization: America Online, Inc. (1-800-827-6364)
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In article <2EA3B0DF@smtp-gateway.cgnet.com>, D.BRAYFORD@CABI.org ("David
Brayford ", IMI) writes:

I work with Medical Mycology specimens.  We have found that they will
store quite well for years when some of the organism is placed in tubes of
sterile water.  Be sure to Parafilm the tubes, however, or the water will
evaporate over time.  I have subcultured from these tubes successfully
after 5 years.  Good luck!


From msachs@ADMIN.OGI.EDU Mon Dec 19 00:31:29 EST 1994
Article: 1209 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!hearst.acc.Virginia.EDU!portal.gmu.edu!europa.eng.gtefsd.com!uhog.mit.edu!grapevine.lcs.mit.edu!olivea!biosci!ADMIN.OGI.EDU!msachs
From: msachs@ADMIN.OGI.EDU (Matthew Sachs)
Newsgroups: bionet.mycology
Subject: Re: Fatty acid synthesis in Neurospora
Date: 14 Dec 1994 10:05:27 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
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Brody, S., and Mikolajczyk, S. (1988). Neurospora mitochondria contain an
acyl-carrier protein. Eur. J. Biochem. 173, 353-359.

Elovson, J. (1975). Purification and properties of the fatty acid
synthetase complex from Neurospora crassa, and the nature of the fas-
mutation.  J. Bacteriol. 124, 524-533.

Henry, S.A., and Keith, A.D. (1971). Saturated fatty acid requirer of
Neurospora crassa.  J. Bacteriol. 106, 174-182

Kionka, C., and Kunau, W. H. (1985). Inducible beta-oxidation pathway in
Neurospora crassa. J. Bacteriol. 161, 153-157.

Mikolajczyk, S., and Brody, S. (1990). De novo fatty acid synthesis
mediated by acyl-carrier protein in Neurospora crassa mitochondria. Eur. J.
Biochem. 187, 431-437.

Thieringer, R., and Kunau, W. H. (1991). The beta-oxidation system in
catalase-free microbodies of the filamentous fungus Neurospora crassa.
Purification of a multifunctional protein possessing 2-enoyl-CoA hydratase,
L-3-hydroxyacyl-CoA dehydrogenase, and 3-hydroxyacyl-CoA epimerase
activities. J. Biol. Chem. 266, 13110-13117.

Zensen, R., Husmann, H., Schneider, R., Peine, T., and Weiss, H. (1992). De
novo synthesis and desaturation of fatty acids at the mitochondrial
acyl-carrier protein, a subunit of NADH:ubiquinone oxidoreductase in
Neurospora crassa. FEBS Lett. 310, 179-181.

-----------------------------------------------------------
Matthew Sachs
Department of Chemistry, Biochemistry and Molecular Biology
Oregon Graduate Institute of Science and Technology
20000 NW Walker Road
P.O. Box 91000
Portland, OR  97291-1000
503 690-1487 Phone
503 690-1464 Fax
msachs@admin.ogi.edu




From nora@molbio.cbs.umn.edu Mon Dec 19 00:31:29 EST 1994
Article: 1210 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!hearst.acc.Virginia.EDU!caen!sol.ctr.columbia.edu!hamblin.math.byu.edu!news.Arizona.EDU!CS.Arizona.EDU!biosci!MANI.CBS.UMN.EDU!npv
From: npv@MANI.CBS.UMN.EDU (Nora Plesofsky-Vig)
Newsgroups: bionet.mycology
Subject: re:fatty acid synthesis in fungi
Date: 14 Dec 1994 10:29:36 -0800
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There are pantothenate auxotrophs of Neurospora crassa that do not  
synthesize fatty acids without supplementation. These mutant strains  
are available from the Fungal Genetics Stock Center at the University  
of Kansas Medical Center, Kansas City, KS 66160-7420. Their e-mail  
address is fgsc@ukanvm.cc.ukans.edu. The Stock Center is a very good  
source of information, strains, and some cloned genes of Neurospora  
and Aspergillus, especially.


From ari@perspective.com Mon Dec 19 00:31:30 EST 1994
Article: 1211 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!rutgers!gatech!howland.reston.ans.net!news.sprintlink.net!news.clark.net!rahul.net!a2i!ari.a2i!ari
From: ari@perspective.com (Ari Kornfeld)
Newsgroups: bionet.mycology
Subject: Different Yeasts?
Date: 17 Dec 1994 22:05:28 GMT
Organization: a2i network
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X-Newsreader: TIN [version 1.2 PL2]


Perhaps someone here could help me with a simple question:

I've gotten as far as figuring out that Saccharomyces cerevisiae is
one of or all of:  baker's yeast, brewers yeast, nutritional yeast.

Could someone point out which it is, and explain the difference
between these items?

  thanks,
-ari


From Rballou@access.digex.net Mon Dec 19 11:44:13 EST 1994
Article: 1212 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!howland.reston.ans.net!news1.digex.net!dcc00693.slip.digex.net!user
From: Rballou@access.digex.net (Rballou)
Newsgroups: bionet.mycology
Subject: Re: "ascomycotina" help
Date: Sun, 18 Dec 1994 21:19:56 +0000
Organization: Express Access Online Communications, USA
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In article <2EF17713@smtp-gateway.cgnet.com>, D.BRAYFORD@CABI.org ("David
Brayford ", IMI) wrote:

> Sorry to sir things up, but the replies concerning classification of Xylaria 
> hypoxylon
> were slightly up the creek, or at least out of date.  The class"Ascomycetes" 
> was got
> rid of many moons ago and replaced by the subdivision "Ascomycotina".   More 
> recently
> the Ascomycotina have been got rid of too - and elevated to the rank of 
> Phylum (= Division).
> There are no classes, just orders and families.  So now we have:
> Kingdom:  Fungi
> Phylum:  Ascomycota
> Order:  Xylariales
> Family:  Xylariaceae
> Genus:  Xylaria
> Species:  hypoxylon
> 
> These changes are enshrined in the latest version of the International Code 
> of Botanical
>  Nomenclature and will be reflected in the forthcoming edition of The 
> Dictionary of the
>  Fungi.
> 
> Hope that's of some help.
> 
> Dave Brayford
> International Mycological Institute
> d.brayford@cabi.org

I appreciate this response. It certainly confirms what I suspected, as
there were a number of different classifications in print. Thanks again to
everybody who chipped in on this one.

Ripley Ballou
Dept of Immunology
WRAIR


From jmicales@facstaff.wisc.edu Mon Dec 19 21:13:48 EST 1994
Article: 1213 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!howland.reston.ans.net!vixen.cso.uiuc.edu!uwm.edu!news.doit.wisc.edu!F181-224.net.wisc.edu!jmicales
From: jmicales@facstaff.wisc.edu (Jessie Micales)
Newsgroups: bionet.mycology
Subject: Re: Cordyceps, Poria
Date: Mon, 19 Dec 1994 22:41:11 GMT
Organization: U.S. Forest Service
Lines: 14
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References: <mgalatz.1138257192A@198.7.0.1>
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>1) Has anyone been able to grow cordyceps on a  non-worm-casing medium.
>2) Has Poria been grown commercially?
>3  Have truffles been grown commercially?  

>mgalatz@panix.com

I must be missing something. I do almost all my work with Postia (new name 
for Poria) placenta.  The fruiting bodies I've always seen are appressed and 
leathery in appearance.  Do people actually eat these things???  If so, 
maybe I've found a commercial application for my research that will please 
our administrators!  If you want some, I'll be happy to sell it to you!  |-)





From mycoworld@aol.com Tue Dec 20 10:56:39 EST 1994
Article: 1214 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!swrinde!pipex!uunet!newstf01.news.aol.com!not-for-mail
From: mycoworld@aol.com (MycoWorld)
Newsgroups: bionet.mycology
Subject: Re: Cordyceps, Poria
Date: 19 Dec 1994 22:58:02 -0500
Organization: America Online, Inc. (1-800-827-6364)
Lines: 24
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Message-ID: <3d5koa$7uu@newsbf02.news-fddi.aol.com>
References: <mgalatz.1138257192A@198.7.0.1>
Reply-To: mycoworld@aol.com (MycoWorld)

mgalatz@panix.com writes:
> 3  Have truffles been grown commercially?

The Nov/Dec 94 issue of Saveur Magazine has a 15 page spread on the
trufffles of Italy (white) and France (black).  It doesn't mention
cultivating them, but focuses on the wild harvest.  The French have been
cultivating them for 20 years or so, apparently by inoculating tree roots
with the fungi.  

There have been two successful growers in the U.S.  Franklin Garland of
Hillsborough, NC (Garland Gourmet Mushrooms and Truffles) planted
inoculated oak and filbert seedlings in 1980 and discovered his first
truffles in October 1993.  Bruce Hatch (with Don Reading and William
Griner) of Ukia, CA planted 100 inoculated flbert trees in 1982 and found
their first truffle in 1992.  I spoke with Bruce recently and he told me
that this year's harvest was about half of last year, on the order of 3
pounds so far.   They do sell the truffles so that makes them commercial,
however even at high prices no one in this country is making a living off
of them.  By the way, Hatch is moving back to Wisconsin and has his
truffle farm on the market.  E-Mail me directly for phone numbers.

Jerry Haugen, Publisher
The Mushroom Growers' Newsletter
MycoWorld@aol.com


From mgalatz@panix.com Tue Dec 20 10:56:41 EST 1994
Article: 1215 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!news-server.ncren.net!taco.cc.ncsu.edu!gatech!bloom-beacon.mit.edu!panix!198!mgalatz
From: mgalatz@panix.com (Menachem Galatz DC)
Newsgroups: bionet.mycology
Subject: Fungi Perfect email?
Followup-To: bionet.mycology
Date: Fri, 16 Dec 94 15:33:43 GMT
Organization: PANIX Public Access Internet and Unix, NYC
Lines: 3
Message-ID: <mgalatz.1137979663B@198.7.0.1>
NNTP-Posting-Host: 166.84.253.221
X-Newsreader: VersaTerm Link v1.1.3

Is there an email address for Fungi Perfect?

mgalatz@panix.com


From rwinder@PFC.Forestry.CA Tue Dec 20 10:56:42 EST 1994
Article: 1216 of bionet.mycology
Newsgroups: bionet.mycology
Path: bigblue.oit.unc.edu!concert!hearst.acc.Virginia.EDU!portal.gmu.edu!europa.eng.gtefsd.com!newsxfer.itd.umich.edu!nntp.cs.ubc.ca!news.UVic.CA!spruce.pfc.forestry.ca!PFC.Forestry.CA!RWINDER
From: rwinder@PFC.Forestry.CA (Richard Winder)
Subject: Re: Classification of Ascomycotina
Message-ID: <1994Dec14.222557.12313@spruce.pfc.forestry.ca>
Sender: news@spruce.pfc.forestry.ca
Nntp-Posting-Host: pfc.pfc.forestry.ca
Reply-To: rwinder@PFC.Forestry.CA
Organization: Forestry Canada (Pacific Forestry Centre)
References: <Rballou-1112941357320001@dcc00693.slip.digex.net>
Date: Wed, 14 Dec 1994 22:25:57 GMT
Lines: 19

In article <Rballou-1112941357320001@dcc00693.slip.digex.net>, 
Rballou@access.digex.net (Rballou) writes:
>I need help on the classification of the fungus Xyleria hypoxylon.
>Specifically can anyone provide me with the order and family?
>
>Thanks - I'm trying to help with my 7th grader's homework!!


Class Ascomycetes
	Order Xylariales
		Family Xylariaceae
			Genus Xylaria
				Species hypoxylon

-RSW

  RICHARD WINDER                    Title: Research Scientist
  Canadian Forest Service           Phone: (604) 363-0773
  Victoria, B.C.                    Internet: RWINDER@A1.PFC.Forestry.CA


From mikek48@aol.com Tue Dec 20 22:11:48 EST 1994
Article: 1217 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!newsfeed.pitt.edu!uunet!newstf01.news.aol.com!not-for-mail
From: mikek48@aol.com (MikeK48)
Newsgroups: bionet.mycology
Subject: POSITION WANTED
Date: 20 Dec 1994 18:56:16 -0500
Organization: America Online, Inc. (1-800-827-6364)
Lines: 7
Sender: root@newsbf02.news.aol.com
Message-ID: <3d7qv0$o1@newsbf02.news.aol.com>
Reply-To: mikek48@aol.com (MikeK48)

I have 2 years experience growing shiitake mushrooms in indoor controlled
environment and am seeking employement in California. If you know of
anyone looking for such a person such as myself please contact me at.

MikeK48@aol.com
or 
(805)834-3112


From grad@D.UMN.EDU Tue Dec 20 22:11:49 EST 1994
Article: 1218 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!bloom-beacon.mit.edu!biosci!D.UMN.EDU!grad
From: grad@D.UMN.EDU (stephen hedman)
Newsgroups: bionet.mycology
Subject: Position Announcement
Date: 19 Dec 1994 15:25:39 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 22
Sender: daemon@net.bio.net
Distribution: world
Message-ID: <199412192325.RAA03577@ub.d.umn.edu>
NNTP-Posting-Host: net.bio.net

Subject: Position Announcement

The Department of Biology at the University of Minnesota-Duluth has a
faculty position at the rank of instructor or assistant professor
available for spring quarter 1995 (March 1 - May 30). The person hired
will teach a molecular biology and genetics lecture course, with course
content appropriate for a text such as Genes V by Lewin; and an
established molecular biology and genetics laboratory course.  An
experienced graduate teaching assistant will be assigned to the
laboratory course.  Applicant must have at least A.B.D. in a relevant
biological speciality, collegiate teaching experience, training in
molecular genetics, and background and experience in bacterial and viral
genetics and recombinant DNA techniques.  Doctoral degree preferred.
Application materials should include: letter of application; curriculum
vitae; undergraduate and graduate transcripts (photocopies acceptable);
names and telephone numbers of three references familiar with
applicant's teaching and experience in molecular genetics.  Applications
must be postmarked by January 24, 1995 to: Donald P. Christian,
Department of Biology, University of Minnesota, Duluth, MN 55812.
Inquiries may be directed to D. Christian, telephone 218/726-7263,
e-mail dchristi@ub.d.umn.edu. The University of Minnesota is an equal
opportunity educator and employer.


From sarai@u.washington.edu Tue Dec 20 22:11:50 EST 1994
Article: 1219 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!howland.reston.ans.net!vixen.cso.uiuc.edu!news.uoregon.edu!netnews.nwnet.net!news.u.washington.edu!microb3.biostat.washington.edu!user
From: sarai@u.washington.edu (Sarah Boomer)
Newsgroups: bionet.mycology
Subject: Re: Clitocybe nuda lookalikes?
Date: Tue, 20 Dec 1994 15:53:20 -0800
Organization: University of Washington
Lines: 22
Message-ID: <sarai-2012941553200001@microb3.biostat.washington.edu>
References: <3d7je9$2v7@darkstar.UCSC.EDU>
NNTP-Posting-Host: microb3.biostat.washington.edu

In article <3d7je9$2v7@darkstar.UCSC.EDU>, taiga@cats.ucsc.edu (Daniel
Jason Katz) wrote:

> Does anyone know if there are any poisonous mushrooms that a novice
could easilymistake for the blewit? I found a bunch of what I think are
Clitocybe nudas
> yesterday in a live oak woodland, and before I munched them, I wanted some
> feedback from experienced mycologists. It fits the description in Mushrooms
> Demystified and All the Rain Promises except that I wouldn't call the stalk
> solid-it's more fibery and hollow. Thanks!
> 
> Dan Katz
> taiga@cats.ucsc.edu

I am not a 'professional' mycologist, but I have gathered C. nuda for
consumption.  I am not aware of any close lookalikes, but other purple
mushrooms do exist, including some in the cortinarius group that are of
uncertain edibility.  Be sure you get a spore print, and be sure they have
that characteristic 'citrusy' smell.  If possible, have someone with more
experience then you look at them.  Remember--when in doubt, throw it out!

Matthew Stoecker


From msachs@ADMIN.OGI.EDU Tue Dec 20 22:11:51 EST 1994
Article: 1220 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!bloom-beacon.mit.edu!biosci!ADMIN.OGI.EDU!msachs
From: msachs@ADMIN.OGI.EDU (Matthew Sachs)
Newsgroups: bionet.mycology
Subject: Re:  Fatty acids in Neurospora -'94 ref
Date: 19 Dec 1994 16:39:51 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 16
Sender: daemon@net.bio.net
Distribution: world
Message-ID: <9412200035.AA06666@admin.ogi.edu>
NNTP-Posting-Host: net.bio.net

Goodrich-Tanrikulu, M., A. E. Stafford, J. T. Lin, M. I. Makapugay, G.
Fuller, and T. A. McKeon. 1994. Fatty acid biosynthesis in novel ufa
mutants of Neurospora crassa. Microbiology 140:2683-2690.

-----------------------------------------------------------
Matthew Sachs
Department of Chemistry, Biochemistry and Molecular Biology
Oregon Graduate Institute of Science and Technology
20000 NW Walker Road
P.O. Box 91000
Portland, OR  97291-1000
503 690-1487 Phone
503 690-1464 Fax
msachs@admin.ogi.edu




From mgalatz@panix.com Wed Dec 21 10:48:42 EST 1994
Article: 1221 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!news-feed-1.peachnet.edu!gatech!bloom-beacon.mit.edu!panix!198!mgalatz
From: mgalatz@panix.com (Menachem Galatz DC)
Newsgroups: bionet.mycology
Subject: Cordyceps, Poria
Followup-To: bionet.mycology
Date: Mon, 19 Dec 94 20:39:12 GMT
Organization: PANIX Public Access Internet and Unix, NYC
Lines: 5
Message-ID: <mgalatz.1138257192A@198.7.0.1>
NNTP-Posting-Host: 166.84.253.221
X-Newsreader: VersaTerm Link v1.1.3

1) Has anyone been able to grow cordyceps on a  non-worm-casing medium.
2) Has Poria been grown commercially?
3  Have truffles been grown commercially?  

mgalatz@panix.com


From P.Hirst@massey.ac.nz Wed Dec 21 10:48:43 EST 1994
Article: 1222 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!howland.reston.ans.net!news.starnet.net!wupost!waikato!comp.vuw.ac.nz!news.massey.ac.nz!PHirst
From: P.Hirst@massey.ac.nz
Newsgroups: bionet.mycology
Subject: Mycosphaerella pini isolates anyone?
Date: Tue, 20 Dec 94 16:01:21 +1200
Organization: Massey University, Palmerston North, New Zealand
Lines: 7
Distribution: world
Message-ID: <20Dec1994.5768148@mg-pc5.massey.ac.nz>
NNTP-Posting-Host: mg-pc5.massey.ac.nz
X-Newsreader: MUNews 1.01

Howdy, I'm doing research in Dothistroma pini (phytopathogen) and am
after some samples of the teleomorph, Mycosphaerella (Scirrhia) pini
to analyse genetic variation between species.  If anyone has any 
information at all on where I might be able to find such a strain, I
would be eternally grateful.

Many thanks, Paul Hirst


From biohelp Wed Dec 21 10:48:45 EST 1994
Article: 1223 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!usenet.ins.cwru.edu!agate!biosci!internet!biosci!not-for-mail
From: biohelp (BIOSCI Administrator)
Newsgroups: bionet.mycology
Subject: UNSUBSCRIBING, BIOSCI ARCHIVES, ADDRESS DATABASE & BIOSCI FAQ
Date: 21 Dec 1994 02:00:16 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 338
Sender: daemon@net.bio.net
Distribution: world
Message-ID: <199412211000.CAA01919@net.bio.net>
NNTP-Posting-Host: net.bio.net


Four important items follow: How to cancel e-mail subscriptions to
BIOSCI newsgroups, BIOSCI archive searching, the BIOSCI FAQ, and the
BIOSCI User Address Directory form.  If you have not yet listed
yourself in our BIOSCI user directory, please take a few minutes to
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updated form.  We can not make manual revisions to existing entries.

				Sincerely,

				Dave Kristofferson
				BIOSCI/bionet Manager

				biosci-help@net.bio.net



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		  Using Gopher to complete the form
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> Data..
> 
> 
> And fill out the form.

or Rob Harper's gopher site in Europe as follows:

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> 
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> 
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> 
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> 
>         1.  Finnish EMBnet BioBox/
> 
>             9.  FAQ Files/
> 
>                 5.  Bionauts Address Database (questionaire) <TEL>
> 



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On the comment: lines
use these names below ---- NOT the USENET names below

MAILING LIST NAME          USENET Newsgroup Name
-----------------          ---------------------
ACEDB-SOFT                 bionet.software.acedb
AGEING                     bionet.molbio.ageing
AGROFORESTRY               bionet.agroforestry
ARABIDOPSIS                bionet.genome.arabidopsis
ASCB                       bionet.prof-society.ascb
BIOCAN                     bionet.prof-society.cfbs
BIOFORUM                   bionet.general
BIO-INFORMATION-THEORY     bionet.info-theory
BIONAUTS                   bionet.users.addresses
BIONEWS                    bionet.announce
BIO-JOURNALS               bionet.journals.contents
BIO-MATRIX                 bionet.molbio.bio-matrix
BIOPHYSICAL-SOCIETY        bionet.prof-society.biophysics
BIOPHYSICS                 bionet.biophysics
BIO-SOFTWARE               bionet.software
BIOTHERMOKINETICS          bionet.metabolic-reg
BIO-WWW                    bionet.software.www
CARDIOVASCULAR-RESEARCH    bionet.biology.cardiovascular
CELEGANS                   bionet.celegans
CELL-BIOLOGY               bionet.cellbiol
CHLAMYDOMONAS              bionet.chlamydomonas
CHROMOSOMES                bionet.genome.chromosomes
COMPUTATIONAL-BIOLOGY      bionet.biology.computational
CSM                        bionet.prof-society.csm
CYTONET                    bionet.cellbiol.cytonet
DROSOPHILA                 bionet.drosophila
EMBL-DATABANK              bionet.molbio.embldatabank
EMF-BIO                    bionet.emf-bio
EMPLOYMENT                 bionet.jobs
EMPLOYMENT-WANTED          bionet.jobs.wanted
FASEB                      bionet.prof-society.faseb
GDB                        bionet.molbio.gdb
GENBANK-BB                 bionet.molbio.genbank
GENETIC-LINKAGE            bionet.molbio.gene-linkage
GRASSES-SCIENCE            bionet.biology.grasses
HIV-MOLECULAR-BIOLOGY      bionet.molbio.hiv
HUMAN-GENOME-PROGRAM       bionet.molbio.genome-program
IMMUNOLOGY                 bionet.immunology
INFO-GCG                   bionet.software.gcg
JOURNAL-NOTES              bionet.journals.note
METHODS-AND-REAGENTS       bionet.molbio.methds-reagnts
MICROBIOLOGY               bionet.microbiology
MOLECULAR-EVOLUTION        bionet.molbio.evolution
MOLECULAR-MODELLING        bionet.molec-model
MOLLUSC-MOLECULAR-NEWS     bionet.molbio.molluscs
MYCOLOGY                   bionet.mycology
NEUROSCIENCE               bionet.neuroscience
N2-FIXATION                bionet.biology.n2-fixation
PARASITOLOGY               bionet.parasitology
PHOTOSYNTHESIS             bionet.photosynthesis
PLANT-BIOLOGY              bionet.plants
POPULATION-BIOLOGY         bionet.population-bio
PROTEIN-ANALYSIS           bionet.molbio.proteins
PROTEIN-CRYSTALLOGRAPHY    bionet.xtallography
PROTISTA                   bionet.protista
RAPD                       bionet.molbio.rapd
SCIENCE-RESOURCES          bionet.sci-resources
STADEN                     bionet.software.staden
STRUCTURAL-NMR             bionet.structural-nmr
TROPICAL-BIOLOGY           bionet.biology.tropical
URODELES                   bionet.organisms.urodeles
VIROLOGY                   bionet.virology
WOMEN-IN-BIOLOGY           bionet.women-in-bio
YEAST                      bionet.molbio.yeast
ZBRAFISH                   bionet.organisms.zebrafish

Listing newsgroups on the comment: line is optional, of course.

Thanks again for your cooperation!



--------------- please cut here and return portion below ---------------

New information or Update to old record (enter N or U): 
date (DD-MM-YY): 
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comment: 
comment: 
comment: 
comment: 
comment: 



From jtaylor@VIOLET.BERKELEY.EDU Wed Dec 21 22:05:41 EST 1994
Article: 1224 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!swrinde!sgiblab!uhog.mit.edu!grapevine.lcs.mit.edu!olivea!biosci!VIOLET.BERKELEY.EDU!jtaylor
From: jtaylor@VIOLET.BERKELEY.EDU (john taylor)
Newsgroups: bionet.mycology
Subject: Classes of Ascomycota
Date: 21 Dec 1994 15:14:05 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 38
Sender: daemon@net.bio.net
Distribution: world
Message-ID: <199412212314.PAA14637@violet.berkeley.edu>
NNTP-Posting-Host: net.bio.net



Regarding David Brayford's posting of 16 Dec 1994 that there are now no
classes of Ascomycota.

        The report on the death of fungal classes, like that of Twain, is
premature.  As we noted in 1992 (Mol. Biol. Evol. 9:278-284) there is
statistically significant support from ribosomal DNA nucleotide sequence
for two groups of Ascomycota that comprise traditional classes, i.e.,
Plectomycetes and Pyrenomycetes.  Other molecular research has supported
these classes, as well as the Hemiascomycetes, and further research is
likely to find others.  Conversely, other traditional classes of Ascomycota
have been shown not to be monophyletic, e.g., Discomycetes and
Loculoascomycetes.

        As noted by Dave Griffin, the international code is a set of rules
for nomenclature, not a listing of the outcomes of the application of these
rules.  Nothing in the rules prohibits specifying classes for Ascomycota.

        In the case of Xylaria species, it is biologically sound to include
them in the well-supported class, Pyrenomycetes (Spatafora and Blackwell,
Mycologia 85:912-922.

Mary Berbee and John Taylor.



____________________________________
From:   John Taylor
        Department of Plant Biology
        111 Koshland Hall
        University of California
        Berkeley, CA  94720
        Tel (510) 642-5366
        Fax (510) 642-4995
        internet jtaylor@violet.berkeley.edu




From griffin@gamera.syr.edu Fri Dec 23 14:05:51 EST 1994
Article: 1225 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!news-server.ncren.net!taco.cc.ncsu.edu!gatech!howland.reston.ans.net!news.cac.psu.edu!news.tc.cornell.edu!travelers.mail.cornell.edu!newstand.syr.edu!gamera.syr.edu!griffin
From: griffin@gamera.syr.edu (David H. Griffin)
Newsgroups: bionet.mycology
Subject: Class Ascomycetes
Date: 19 Dec 1994 15:11:15 GMT
Organization: Syracuse University, Syracuse, NY
Lines: 55
Distribution: world
Message-ID: <3d47qj$mn5@newstand.syr.edu>
NNTP-Posting-Host: gamera.syr.edu

Concerning the use of the International Code of Botanical Nomenclature, I 
have quite a different understanding from that of Dave Brayford
who wrote:

 "Sorry to stir things up, but the replies concerning classification of 
Xylaria hypoxylon
were slightly up the creek, or at least out of date.  The class"Ascomycetes"
was got
rid of many moons ago and replaced by the subdivision "Ascomycotina".   More
recently
the Ascomycotina have been got rid of too - and elevated to the rank of
Phylum (= Division).
There are no classes, just orders and families.  So now we have:
Kingdom:  Fungi
Phylum:  Ascomycota
Order:  Xylariales
Family:  Xylariaceae
Genus:  Xylaria
Species:  hypoxylon

These changes are enshrined in the latest version of the International Code
of Botanical
 Nomenclature and will be reflected in the forthcoming edition of The
Dictionary of the
 Fungi.

Hope that's of some help.

Dave Brayford
International Mycological Institute
d.brayford@cabi.org"

According to the 1988 edition of the code, Division II, Article 3 states:

"3.1. The principal ranks of taxa in ascending sequence are : species 
(species), genus (genus), family (familia), order (ordo), class 
(classis), division (divisio), and kingdom (regnum)."

There is no statement in the code that I can find that "classes" of fungi 
are not recognized, nor any that states what the names of recognized 
divisions shall be. There is no recognition of the term "phyllum".

It is my understanding that the code treats naming of taxa and does not 
treat the development or acceptance of any particular taxonomic systems 
involving the use of these names. Thus, the code specifically does not 
legislate that the Ascomycetes shall not be treated as a class, but must 
be treated as a Division called Ascomycota. This kind of decision is a 
matter of taxonomic opinion, left to the individual "taxonomist"; there 
are many of these.

David H. Griffin
SUNY College of Environmental Science and Forestry
Syracuse NY 13210
griffin@mailbox.syr.edu



From rarnold@teleport.com Fri Dec 23 14:05:52 EST 1994
Article: 1226 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!rutgers!gatech!howland.reston.ans.net!agate!library.ucla.edu!psgrain!agora!rainrgnews0!news.teleport.com!usenet
From: "Ralph D. Arnold" <rarnold@teleport.com>
Newsgroups: bionet.mycology
Subject: First Week of FUNGUS archived
Date: Wed, 21 Dec 94 20:25:26 PST
Organization: Teleport - Portland's Public Access (503) 220-1016
Lines: 23
Message-ID: <83606.rarnold@teleport.com>
Reply-To: <rarnold@teleport.com>
NNTP-Posting-Host: ip-pdx2-10.teleport.com
X-Minuet-Version: Minuet1.0_Beta_17A
X-POPMail-Charset: English
To: fungus@teleport.com

The email messages of the first week of FUNGUS, the Internet mailing list
dedicated to mushroom cultivation (both hobby & comm'l) and mycology
resources have been placed in the permenant archive site.

It is reachable via anonymous FTP at ftp.teleport.com in directory:
   \pub\users\rarnold\mushroom\FUNGUS

Week 1 (file 1294A.txt) contains 158.7 K of info, including details for
growing Shiitake, Lion's Mane, several announcements and info on resources,
plus quite a bit of misc. info.

To Subscribe (free) or Unsubscribe, Send an email to:
fungus-request@teleport.com 
with message SUBSCRIBE (or UNSUBSCRIBE) FUNGUS <you@your.domain>

To Send a Message/Comment/Question/Answer/Textfile, send it to:
fungus@teleport.com

Happy Holidays!!

Ralph D. Arnold rarnold@teleport.com 
P.O. Box 889, Oregon City, OR 97045   
fax: 503-423-7409


From ari@perspective.com Fri Dec 23 14:05:53 EST 1994
Article: 1227 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!news-server.ncren.net!taco.cc.ncsu.edu!gatech!swrinde!sgiblab!rahul.net!a2i!ari.a2i!ari
From: ari@perspective.com (Ari Kornfeld)
Newsgroups: bionet.molbio.yeast,bionet.mycology
Subject: Re: Different Yeasts?
Date: 20 Dec 1994 20:18:03 GMT
Organization: a2i communications
Lines: 19
Message-ID: <3d7e5r$6p5@hustle.rahul.net>
References: <3cu4jv$ikj@hustle.rahul.net>
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NNTP-Posting-User: ari
X-Newsreader: TIN [version 1.2 PL2]
Xref: bigblue.oit.unc.edu bionet.molbio.yeast:1904 bionet.mycology:1227

Ari Kornfeld (ari@perspective.com) wrote:

: I've gotten as far as figuring out that Saccharomyces cerevisiae is
: one of or all of:  baker's yeast, brewers yeast, nutritional yeast.

Thanks for your replies:

  The concensus seems to be that S. cerevisiae is baker's &
nutritional yeast (since nut. yeast is just a processed form of
baker's yeast) and that brewer's yeast may either be S. carlsbergensis
or S. cerevisiae.

  One person even pointed out that spanish for beer is cervesa - named
after the little bugger, no doubt.

  So does that sound right?  Any dissenting opinions?

  thanks again
-ari


From D.BRAYFORD@CABI.org Fri Dec 23 14:05:54 EST 1994
Article: 1228 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!howland.reston.ans.net!agate!biosci!CABI.org!D.BRAYFORD
From: D.BRAYFORD@CABI.org ("David Brayford ", IMI)
Newsgroups: bionet.mycology
Subject: FW: Manipulations with Filamentous Fungi
Date: 20 Dec 1994 23:58:00 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 18
Sender: daemon@net.bio.net
Distribution: world
Message-ID: <2EF7DFDF@smtp-gateway.cgnet.com>
NNTP-Posting-Host: net.bio.net


Erm, excuse me, but I certainly didn't write this:
Dave Brayford
 ----------
From: BIOSCI-REQUEST
To: mycology
Subject: Re: Manipulations with Filamentous Fungi
Date: 17 December 1994 3:35

In article <2EA3B0DF@smtp-gateway.cgnet.com>, D.BRAYFORD@CABI.org ("David
Brayford ", IMI) writes:

I work with Medical Mycology specimens.  We have found that they will
store quite well for years when some of the organism is placed in tubes of
sterile water.  Be sure to Parafilm the tubes, however, or the water will
evaporate over time.  I have subcultured from these tubes successfully
after 5 years.  Good luck!



From arnold@bscr.uga.edu Fri Dec 23 14:05:54 EST 1994
Article: 1229 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!news-server.ncren.net!taco.cc.ncsu.edu!biosci!GENOME.GENETICS.UGA.EDU!arnold
From: arnold@GENOME.GENETICS.UGA.EDU
Newsgroups: bionet.mycology
Subject: (none)
Date: 20 Dec 1994 07:12:22 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 111
Sender: daemon@net.bio.net
Distribution: world
Message-ID: <0098937A.A0EAB811.30@genome.genetics.uga.edu>
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NNTP-Posting-Host: net.bio.net

			MYCOLOGY AT GEORGIA

Mycology at Georgia:  The Mycology Program is unique in the U.S. for its
strength and diversity, and has the only NIH-funded Mycology Training Grant
supporting Predoctoral students.  In addition to the faculty listed below,
there are adjunct faculty who can serve as rotation advisors.  These include
Dr. Tim Lott (Centers for Disease Control),  Dr. David Logan
(Clark-Atlanta University), Dr. Steven Y. Newell (UGA Marine 
Institute), and Dr. Charles Bacon (USDA).

FACULTY

Jonathan Arnold (Genetics Department)
Physical mapping and evolution of fungal genomes

Sarah F. Covert (School of Forest Resources)
Molecular biology of fungal plant pathogens; mechanisms of pathogenesis
and host specificity

Karl-Erik L. Eriksson (Biochemistry and Molecular Biology Department)
Research in lignin biodegradation; degradation of wood polysaccharides;
study of bacteria isolated from white-rot fungi

Claiborne Glover (Biochemistry and Molecular Biology Department)
Biochemistry of protein phorphorylation and cell cycle regulation
in Saccharomyces cerevisiae

Scott E. Gold (Plant Pathology Department)
Genetics of dimorphism and pathogenicity in the corn smut fungus
Ustilago maydis

Michael G. Hahn (Complex Carbohydrate Research Center)
Plant receptors for fungal cell wall-derived oligoglucosides that
induce plant defenses; immunological studies of fungal cell walls

Richard T. Hanlin (Plant Pathology Department)
Developmental morphology of ascomycetes and their conidial states; biology
of plant pathogenic perithecioid ascomycetes

Charles W. Mims (Plant Pathology Department)
Ultrastructural studies of sporogenesis in fungi and of
host-pathogen relationships in fungal diseases of plants

David Porter (Botany Department)
Biology and phylogeny of zoosporic marine fungi and slime molds.
Fungal parasites of seagrasses


PROGRAMS

The Mycology Program at the University of Georgia has 14 faculty members
with research interests covering a broad range of topics in modern mycology.
Some of the areas of particular emphasis include:

- Genetics and Molecular Biology
- Biochemistry and Physiology
- Morphology
- Evolution

FACILITIES

The Mycology Program, spanning the Departments of Genetics, Biochemistry,
Botany, Forestry, and Plant Pathology, has excellent support
facilities for research programs including:

- 28,000 sq. ft of greenhouse and growth chambers
- large herbarium collection
- NSF/DOE/USDA-funded BioScience Computational Resource for DNA sequence 
analysis, molecular modeling, and evolutionary analyses
- NSF/DOE-funded Molecular Genetics Instrumentation Facility (MGIF) for
DNA and protein sequencing, DNA and peptide synthesis, and peptide purification
- Monoclonal Antibody Facility
- NSF-funded ultrastructural research facility with state-of-the-art
electron and confocal microscopy equipment
- DOE-funded Complex Carbohydrate Research Center (CCRC) with mass spectrometry 
and NMR facilities

Graduate Studies and Postdoctoral Research
Funding is available from a variety of sources to support graduate students
and postdoctoral researchers, including NIH Mycology training grants,
fellowships, and research and teaching assistantships.

For more information on the Mycology Program at the University, mail in
one of the cutouts below or access UGA Gopher or call Michael Hahn
at (706) 542-4457.

For more information on postdoctoral opportunities, write directly or
send an email to the faculty member in whom you have an interest. 
The mailing address is _________ Department, University of Georgia,
Athens, GA 30602.  Email addresses are listed under 
the UGA Gopher.

--------------------------------------------------------------------------------


Please send me more information, and an application package, for
graduate studies in the Mycology Program.


Area of Interest: ___________________
Name: __________________________
Address: ________________________

         ________________________

         ________________________
Graduate Studies Coordinator
Mycology Program
CCRC
University of Georgia
Athens, GA 30602


From tjvolk@facstaff.wisc.edu Fri Dec 23 14:05:55 EST 1994
Article: 1230 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!news-server.ncren.net!taco.cc.ncsu.edu!gatech!howland.reston.ans.net!news.moneng.mei.com!uwm.edu!news.doit.wisc.edu!F181-087.net.wisc.edu!tjvolk
From: tjvolk@facstaff.wisc.edu (Tom Volk)
Newsgroups: bionet.mycology
Subject: Re: Cordyceps, Poria
Date: Tue, 20 Dec 1994 15:16:03 GMT
Organization: U.S. Forest Service
Lines: 23
Message-ID: <tjvolk.65.2EF6F532@facstaff.wisc.edu>
References: <mgalatz.1138257192A@198.7.0.1>
NNTP-Posting-Host: f181-087.net.wisc.edu

In article <mgalatz.1138257192A@198.7.0.1> mgalatz@panix.com (Menachem Galatz DC) writes:

>2) Has Poria been grown commercially?

The genus Poria Adans. (1763) has been rejected as a nomen dubium (
see Ryvarden 1985 Mycotaxon 23:169-198) as has Poria Pers. (1794)  rejected 
as a later homonym of Poria Adans. (1763)

The genus Poria was used for most of this century for any resupinate 
polypore, most of which were not closely related to one another.  Its 
members are now distributed in 40-50 other genera.

The "Poria" you are asking about growing must be Poria cocos (Schw.) Wolf, 
current name Wolfiporia cocos (Schw.) Ryv. & Gilbn.  It forms a large 
underground sclerotium (tuckahoe) which can be eaten.  The mycelium is easy 
to grow in culture, but to my knowledge large sclerotia have not been 
grown.  Someone probably has done it though.  I've never eaten it, so it may 
be a worthwhile project for someone.

hope this helps.
---Tom Volk
Center for Forest Mycology Research
Forest Products Lab, Madison WI


From mgalatz@panix.com Fri Dec 23 14:05:56 EST 1994
Article: 1231 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!bloom-beacon.mit.edu!panix!198!mgalatz
From: mgalatz@panix.com (Menachem Galatz DC)
Newsgroups: bionet.mycology
Subject: blue cheeze mold
Followup-To: bionet.mycology
Date: Thu, 22 Dec 94 23:30:55 GMT
Organization: PANIX Public Access Internet and Unix, NYC
Lines: 4
Message-ID: <mgalatz.1138526695A@198.7.0.1>
NNTP-Posting-Host: 166.84.253.221
X-Newsreader: VersaTerm Link v1.1.3

Are there any internet or other resources on the culturing and production of
various cheezes: blue, camembert, etc?

mgalatz@panix.com


From taiga@cats.ucsc.edu Fri Dec 23 14:05:57 EST 1994
Article: 1232 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!news-server.ncren.net!taco.cc.ncsu.edu!gatech!swrinde!cs.utexas.edu!uunet!olivea!news.hal.COM!darkstar.UCSC.EDU!cats.ucsc.edu!taiga
From: taiga@cats.ucsc.edu (Daniel Jason Katz)
Newsgroups: bionet.mycology
Subject: Clitocybe nuda lookalikes?
Date: 20 Dec 1994 21:47:53 GMT
Organization: University of California, Santa Cruz
Lines: 10
Message-ID: <3d7je9$2v7@darkstar.UCSC.EDU>
NNTP-Posting-Host: am.ucsc.edu
X-Newsreader: NN version 6.5.0 #1 (NOV)


Does anyone know if there are any poisonous mushrooms that a novice could easilymistake for the blewit? I found a bunch of what I think are Clitocybe nudas
yesterday in a live oak woodland, and before I munched them, I wanted some
feedback from experienced mycologists. It fits the description in Mushrooms
Demystified and All the Rain Promises except that I wouldn't call the stalk
solid-it's more fibery and hollow. Thanks!

Dan Katz
taiga@cats.ucsc.edu



From mike@clpc4.icos.com Fri Dec 23 14:05:58 EST 1994
Article: 1233 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!news-server.ncren.net!taco.cc.ncsu.edu!gatech!howland.reston.ans.net!news.sprintlink.net!nwnexus!news.halcyon.com!usenet
From: mike@clpc4.icos.com
Newsgroups: bionet.mycology
Subject: Re: Different Yeasts?
Date: Tue, 20 Dec 94 15:32:38 PDT
Organization: Northwest Nexus Inc.
Lines: 36
Message-ID: <3d7pti$sm5@news.halcyon.com>
References: <3cvnb8$g6h@hustle.rahul.net>
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Mime-Version: 1.0
Content-Type: TEXT/PLAIN; charset=US-ASCII
X-Newsreader: NEWTNews & Chameleon -- TCP/IP for MS Windows from NetManage


In article <3cvnb8$g6h@hustle.rahul.net>, <ari@perspective.com> writes:
> Path: 
news.halcyon.com!nwnexus!scipio.cyberstore.ca!nntp.cs.ubc.ca!newsxfer.itd.umich
edu!gatech!howland.reston.ans.net!news.sprintlink.net!news.clark.net!rahul.net
!a2i!ari.a2i!ari
> From: ari@perspective.com (Ari Kornfeld)
> Newsgroups: bionet.mycology
> Subject: Different Yeasts?
> Date: 17 Dec 1994 22:05:28 GMT
> Organization: a2i network
> Lines: 11
> Message-ID: <3cvnb8$g6h@hustle.rahul.net>
> NNTP-Posting-Host: foxtrot.rahul.net
> NNTP-Posting-User: ari
> X-Newsreader: TIN [version 1.2 PL2]
> 
> 
> Perhaps someone here could help me with a simple question:
> 
> I've gotten as far as figuring out that Saccharomyces cerevisiae is
> one of or all of:  baker's yeast, brewers yeast, nutritional yeast.
> 
> Could someone point out which it is, and explain the difference
> between these items?
> 
>   thanks,
> -ari

Same organism, but different strains.  Baker's yeast have been selected for 
their ability to release gases (CO2) into bread type products while brewer's 
yeast were selected for their ability to produce ethanol and flavor.  Just like 
apples (Granny Smith, Fiji, Red Delicious, etc...) they're all basically the 
same.

Mike


From mgkmkr@aol.com Fri Dec 23 14:05:59 EST 1994
Article: 1234 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!swiss.ans.net!newstf01.news.aol.com!not-for-mail
From: mgkmkr@aol.com (MGKMKR)
Newsgroups: bionet.mycology
Subject: Mycology Society
Date: 23 Dec 1994 07:37:11 -0500
Organization: America Online, Inc. (1-800-827-6364)
Lines: 4
Sender: root@newsbf02.news.aol.com
Message-ID: <3deg9n$66j@newsbf02.news.aol.com>
Reply-To: mgkmkr@aol.com (MGKMKR)

Medical Mycology Society of New York
All those interested in joining informal
discussions and lectures with guest speakers,
please contact. 


From moldgrower@aol.com Sat Dec 24 00:07:54 EST 1994
Article: 1235 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!rutgers!gatech!swiss.ans.net!newstf01.news.aol.com!not-for-mail
From: moldgrower@aol.com (Moldgrower)
Newsgroups: bionet.mycology
Subject: Dematiaceous fungi
Date: 23 Dec 1994 01:06:51 -0500
Organization: America Online, Inc. (1-800-827-6364)
Lines: 7
Sender: root@newsbf02.news.aol.com
Message-ID: <3ddpdr$4qb@newsbf02.news.aol.com>
Reply-To: moldgrower@aol.com (Moldgrower)

I have isolated a black, slow-growing mold from a patient's eye.  I have
been unable to get it to sporulate and therefore cannot ID it.  Have sent
it to the State Dept of Health...also unable to ID it.  Anyone able to
help please send E mail to:
Moldgrower@aol . com

Thanks in advance


From ad727@rgfn.epcc.Edu Sat Dec 24 00:07:55 EST 1994
Article: 1236 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!bloom-beacon.mit.edu!uhog.mit.edu!grapevine.lcs.mit.edu!olivea!biosci!RGFN.EPCC.EDU!ad727
From: ad727@RGFN.EPCC.EDU (Lester Pasarell)
Newsgroups: bionet.mycology
Subject: re: dematiaceous fungi
Date: 23 Dec 1994 10:01:01 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 49
Sender: daemon@net.bio.net
Distribution: world
Message-ID: <9412231800.AA18930@rgfn.epcc.Edu>
Reply-To: ad727@rgfn.epcc.Edu



      
    Date: Fri, 23 Dec 94
    
    
    Date: Fri, 23 Dec 94 10:53:48 MST
    Message-Id: <9412231753.AA18277@rgfn.epcc.Edu>
    From: ad727 (Lester Pasarell)
    To: moldgrower@aol
    Subject: Re: Dematiaceous fungi
    Reply-To: ad727@rgfn.epcc.Edu
    
    
    
    >
    >I have isolated a black, slow-growing mold from a patient's eye.  I have
    >been unable to get it to sporulate and therefore cannot ID it.  Have sent
    >it to the State Dept of Health...also unable to ID it.  Anyone able to
    >help please send E mail to:
    >Moldgrower@aol . com
    >
    >Thanks in advance
    
    
    Hi! We would be very happy to assist you in the identification of this or
    any other Dematiaceous fungus, Our Boss, Jefe or Patron is an expert in the
    field of clinically important dematiaceous hyphomycetes= Dr Michael R McGinnis
    you can send the culture to his attention or to my attention at:
    
    Medical Mycology Research Laboratory
    Center for Tropical Diseases
    Department of Pathology
    University of Texas Medical Branch
    301 University Blvd
    Galveston, Texas  77555-0609
    
    bye
    
    Lester Pasarell
    ad727@rgfn.epcc.edu
    
    
    
    
   
   
    
    


From kellogg@raven.csrv.uidaho.edu Sat Dec 24 10:45:35 EST 1994
Article: 1237 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!hearst.acc.Virginia.EDU!caen!uwm.edu!vixen.cso.uiuc.edu!news.uoregon.edu!netnews.nwnet.net!owl.csrv.uidaho.edu!raven.csrv.uidaho.edu!kellogg
From: kellogg@raven.csrv.uidaho.edu (Scott Kellogg)
Newsgroups: bionet.mycology
Subject: Lab Manuals?
Date: 22 Dec 1994 22:19:11 GMT
Organization: University of Idaho, Moscow, Idaho
Lines: 8
Distribution: world
Message-ID: <3dcu0v$44m@owl.csrv.uidaho.edu>
NNTP-Posting-Host: raven.csrv.uidaho.edu
X-Newsreader: TIN [version 1.2 PL2]

What are some recommendations for possible lab manuals that are still in 
print for a upper division/graduate lab course in general mycology?  Thanks.

--
=========================================
Scott T. Kellogg, Dept. of Microbiol., Mol. Biol. & Biochem.
Univ. of Idaho, Moscow, ID 83843 Internet: kellogg@uidaho.edu
Phone: 208-885-6966      Fax: 208-885-6518


From stamets1@aol.com Tue Dec 27 22:57:56 EST 1994
Article: 1238 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!swrinde!pipex!uunet!newstf01.news.aol.com!not-for-mail
From: stamets1@aol.com (Stamets 1)
Newsgroups: bionet.mycology
Subject: Source for collapsable, epoxy mushroom hunting basket
Date: 24 Dec 1994 15:13:38 -0500
Organization: America Online, Inc. (1-800-827-6364)
Lines: 16
Sender: root@newsbf02.news.aol.com
Message-ID: <3dhvdi$fgo@newsbf02.news.aol.com>
Reply-To: stamets1@aol.com (Stamets 1)

Fellow Friends of the Fungus

Years ago, we had a supplier from L.A. for an epoxy covered, collapsable
mushroom hunting basket. We have long since lost their address. Does
anyone know where we can source these? Ron Petersen got the last
of our stock. We very much appreciate any leads. Thanks.
 
paul stamets

ps: FYI - a malfunction destroyed all inbound e-mail from 12/21-12/22.
 the message flashed something about "instability" and then zapped out 4-5
letters which were awaiting delivery. 
can not be retrieved. 


Merry Christmas ! My your hyphae branch and dikaryotize with abandon! 


From stamets1@aol.com Tue Dec 27 22:57:56 EST 1994
Article: 1239 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!swrinde!pipex!uunet!newstf01.news.aol.com!not-for-mail
From: stamets1@aol.com (Stamets 1)
Newsgroups: bionet.mycology
Subject: heat-resistant bacteria fouling pure cultures of fungi
Date: 24 Dec 1994 15:36:32 -0500
Organization: America Online, Inc. (1-800-827-6364)
Lines: 54
Sender: root@newsbf02.news.aol.com
Message-ID: <3di0og$fjc@newsbf02.news.aol.com>
Reply-To: stamets1@aol.com (Stamets 1)

Fellow Mycologists

On heat resistant bacteria fouling pure cultures:

Just what could be a helpful note. Most of you making culture
media for fungi autoclave for 30-45 minutes at 15 psi (1kg/cm-2).
Most use wisely use filtered/purified water. I usually do not. 
With the massive rains we have had here in the
 past 3 weeks - the worst flooding in 75 years - 
the ground water has become
unusually rich with sediments/nutrients,
 causing a bacterial bloom, an order of magnitude
 greater than that which I have seen in years.
 Running parallel trials, I was shocked to find
that malt extract agar media "spontaneously"
 contaminated with bacteria after 2 1/2 hours of autoclaving.
 The contaminating agents appeared 3 weeks after pouring.
 So goes the concept of "sterilization".
 Clearly, the more contaminants at the front end of the
process, the more which survive, post treatment.
 We have had to return to osmosis-filtered water,
 which, for a production facility is not without its limitations. 

A friend in Europe, Peter Oei, author of several books on 
mushroom cultivation, e-mailed me with what appears to be
a similar  problem. Collectively, we have more than 40 years
of culture experience. This is the first time we both have 
seen this. I can only wonder - Could bacteria with greater
 heat tolerance be evolving? Is world-wide ground water contamination and
nutrient flow creating a new environment for the evolution of
these bacteria? 

   How many physcians really comprehend what "sterilization"
means. Most hospital disposal programs for destruction of pathogens
would not compare favorably with my experiences.Do I sound alarmist?
Are my concerns unjustified, unscientifically based. I invite your 
comments - especially from bacteriologists. 

     On another matter, have any of you heard about
 "gene migration" of antibiotic-resistant properties of bacteria
 which have not been directly to antibiotics exposed? 

    Evolution is a powerful force. I can only wonder at what
unwitting participation humans, laboratories, hospitals, day
care centers, and immunized populations play in the evolution
of new generation pathogens.

 On that hopeful note, I remain humbled by the power of the
invisible landscape. 

Happy New Year! 

paul stamets
 


From stweiss@eis.calstate.edu Tue Dec 27 22:57:57 EST 1994
Article: 1240 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!bloom-beacon.mit.edu!usc!nic-nac.CSU.net!ctp.org!not-for-mail
From: stweiss@eis.calstate.edu (Steve Weiss)
Newsgroups: bionet.mycology
Subject: Wild Mushrooms in So. Cal.?
Date: 25 Dec 1994 11:36:03 -0800
Organization: California Technology Project of The Calif State Univ
Lines: 10
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NNTP-Posting-Host: eis.calstate.edu

Over the holidays, my inlaws from Barcelona are staying with me in Los 
Angeles.  They would like to look for mushrooms in this area.  They 
can distinguish mushrooms such as Lactarius sanguifluus, Morchella 
vulgaris, and Cantharelus cibarius.  Any suggestions as to where in 
Southern California I might take them?  You can email me directly.

Thanks in advance,

Steve Weiss
stweiss@eis.calstate.edu


From st000533@hrz.th-darmstadt.de Tue Dec 27 22:57:58 EST 1994
Article: 1241 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!swiss.ans.net!news.dfn.de!zeus.rbi.informatik.uni-frankfurt.de!terra.wiwi.uni-frankfurt.de!news.th-darmstadt.de!rs22.hrz.th-darmstadt.de!st000533
From: st000533@hrz.th-darmstadt.de (Wolf Lichtenberger)
Newsgroups: bionet.mycology,bionet.general,sci.bio.microbiology
Subject: Expression of (fungus) gene on different loci ?
Date: 25 Dec 1994 22:41:07 GMT
Organization: Technische Hochschule Darmstadt
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Hello folks of the scientific community,
on behalf of a friend working on this topic i post a question for him.
As usual, i will post a summary of any postings to the net in due time.
Here he goes:

     I'm sure that a gene varies it's protein production rate if situated 
   on different loci. Any experiences in the field of gene expression 
   variation at the transcription level would be welcome, especially with 
   fungi (filamentous fungi would be even better :-) )

Thanx a lot for the expected flood of replies 8-)
	Wolf
<fancy .sig under reconstruction> [in real life Wolf Lichtenberger,CS major]


From peter@well.sf.ca.us Tue Dec 27 22:57:59 EST 1994
Article: 1242 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!howland.reston.ans.net!pipex!uunet!decwrl!olivea!barrnet.net!well!peter
From: peter@well.sf.ca.us (Peter Febbroriello)
Newsgroups: bionet.mycology
Subject: Plasmopara
Date: 26 Dec 1994 01:56:35 GMT
Organization: The Whole Earth 'Lectronic Link, Sausalito, CA
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NNTP-Posting-Host: well.sf.ca.us


Having found a very interesting strain of Plasmopara, I would like
some assistance in speciating it. Anyone interested...please email me at
2448141@mcimail.com
If you coax me, I might elaborate on where this was found!
--pf


From gordonr@wimsey.com Tue Dec 27 22:58:00 EST 1994
Article: 1243 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!howland.reston.ans.net!agate!library.ucla.edu!psgrain!nntp.cs.ubc.ca!unixg.ubc.ca!vanbc.wimsey.com!pme29.bby.wis.net!gordonr
From: gordonr@wimsey.com (Gordon Robertson)
Newsgroups: bionet.mycology
Subject: Looking for a thesis
Date: Sun, 25 Dec 1994 13:16:18
Organization: PAPRICAN
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NNTP-Posting-Host: pme29.bby.wis.net
X-Newsreader: Trumpet for Windows [Version 1.0 Rev A]

Does anyone from the Southeastern Missouri State University know where to 
find the Master thesis of D.F. Venable on " Subcellular localization of 
protease activity in Schizophyllum commune"?  Has this work been published?

Thanks in advance for your help.  



From gryyne@aol.com Tue Dec 27 22:58:01 EST 1994
Article: 1244 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!swrinde!pipex!uunet!newstf01.news.aol.com!not-for-mail
From: gryyne@aol.com (Gryyne)
Newsgroups: bionet.mycology
Subject: Re: First Week of FUNGUS archived
Date: 27 Dec 1994 16:17:37 -0500
Organization: America Online, Inc. (1-800-827-6364)
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Reply-To: gryyne@aol.com (Gryyne)

i WOULD like to subscribe, thank you.\

gryyne@aol


From andrzej@UIDAHO.EDU Tue Dec 27 22:58:02 EST 1994
Article: 1245 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!howland.reston.ans.net!agate!biosci!UIDAHO.EDU!andrzej
From: andrzej@UIDAHO.EDU (Paszczynski Andrzej)
Newsgroups: bionet.mycology
Subject: Re: Heat resistant bacteria???
Date: 27 Dec 1994 10:18:08 -0800
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NNTP-Posting-Host: net.bio.net



On Sat, 24 Dec 1994 VMUTIL@IDUI1.CSRV.UIDAHO.EDU wrote:

> ============================================================
> Received: from net.bio.net by IDUI1.CSRV.UIDAHO.EDU (IBM VM SMTP V2R1) with TCP;
>    Sat, 24 Dec 94 12:42:49 PST
> Received: (from daemon@localhost) by net.bio.net (8.6.9/8.6.6) id MAA02948 for mycology-list; Sat, 24 Dec 1994 12:35:17 -0800
> Received: (from news@localhost) by net.bio.net (8.6.9/8.6.6) id MAA02931 for mycology-arpanet; Sat, 24 Dec 1994 12:35:16 -0800
> To: mycology@net.bio.net
> From: stamets1@aol.com (Stamets 1)
> Subject: heat-resistant bacteria fouling pure cultures of fungi
> Date: 24 Dec 1994 15:36:32 -0500
> Sender: root@newsbf02.news.aol.com
> Message-ID: <3di0og$fjc@newsbf02.news.aol.com>
> Reply-To: stamets1@aol.com (Stamets 1)
> 
> Fellow Mycologists
> 
> On heat resistant bacteria fouling pure cultures:
> 
> Just what could be a helpful note. Most of you making culture
> media for fungi autoclave for 30-45 minutes at 15 psi (1kg/cm-2).
> Most use wisely use filtered/purified water. I usually do not.
> With the massive rains we have had here in the
>  past 3 weeks - the worst flooding in 75 years -
> the ground water has become
> unusually rich with sediments/nutrients,
>  causing a bacterial bloom, an order of magnitude
>  greater than that which I have seen in years.
>  Running parallel trials, I was shocked to find
> that malt extract agar media "spontaneously"
>  contaminated with bacteria after 2 1/2 hours of autoclaving.
>  The contaminating agents appeared 3 weeks after pouring.
>  So goes the concept of "sterilization".
>  Clearly, the more contaminants at the front end of the
> process, the more which survive, post treatment.
>  We have had to return to osmosis-filtered water,
>  which, for a production facility is not without its limitations.
> 
> A friend in Europe, Peter Oei, author of several books on
> mushroom cultivation, e-mailed me with what appears to be
> a similar  problem. Collectively, we have more than 40 years
> of culture experience. This is the first time we both have
> seen this. I can only wonder - Could bacteria with greater
>  heat tolerance be evolving? Is world-wide ground water contamination and
> nutrient flow creating a new environment for the evolution of
> these bacteria?
> 
>    How many physcians really comprehend what "sterilization"
> means. Most hospital disposal programs for destruction of pathogens
> would not compare favorably with my experiences.Do I sound alarmist?
> Are my concerns unjustified, unscientifically based. I invite your
> comments - especially from bacteriologists.
> 
>      On another matter, have any of you heard about
>  "gene migration" of antibiotic-resistant properties of bacteria
>  which have not been directly to antibiotics exposed?
> 
>     Evolution is a powerful force. I can only wonder at what
> unwitting participation humans, laboratories, hospitals, day
> care centers, and immunized populations play in the evolution
> of new generation pathogens.
> 
>  On that hopeful note, I remain humbled by the power of the
> invisible landscape.
> 
> Happy New Year!
> 
> paul stamets
> 
> 
Friendly suggestion,
It is very easy to draw conclusion like above. I suggest to check 
performance of your autoclave by using internal mercury termometer. You 
can by autoclave termometer from VWR.
 
Andrzej Paszczynski University of Idaho   
Andrzej@crow.csrv.uidaho.edu



From sandy@icr.pt.cyanamid.COM Tue Dec 27 22:58:03 EST 1994
Article: 1246 of bionet.mycology
Newsgroups: bionet.molbio.yeast,bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!howland.reston.ans.net!newsserver.jvnc.net!cyanamid!igate.cyanamid.com!sandy
From: sandy@icr.pt.cyanamid.COM (Sandy Silverman)
Subject: Re: Different Yeasts?
In-Reply-To: ari@perspective.com's message of 20 Dec 1994 20:18:03 GMT
Message-ID: <SANDY.94Dec27161010@icr.pt.cyanamid.COM>
Sender: news@cyanamid.uucp
Organization: American Cyanamid Company
References: <3cu4jv$ikj@hustle.rahul.net> <3d7e5r$6p5@hustle.rahul.net>
Date: Tue, 27 Dec 1994 21:10:10 GMT
Lines: 5
Xref: bigblue.oit.unc.edu bionet.molbio.yeast:1918 bionet.mycology:1246

I thought "nutritional" yeast was Torula.
--
Sanford Silverman                      >Opinions expressed here are my own<
American Cyanamid  
silvermans@pt.cyanamid.com     			"Yeast is Best"


From MULROY@EPISYS.EPI.WISC.EDU Wed Dec 28 23:03:12 EST 1994
Article: 1247 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!decwrl!news.doit.wisc.edu!news
From: MULROY@EPISYS.EPI.WISC.EDU (Bob Mulroy)
Newsgroups: bionet.mycology
Subject: Kombu-cha
Date: 28 Dec 1994 07:59:42 GMT
Organization: National Masturbation Advisory Council
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X-News-Reader: VMS NEWS 1.24

	I'm really really really very sorry about this.  

        I just went over to Yaohan, -the Asian mall in the Chicago Burbs, and
YES, KOMBU-CHA is a tea made of powdered giant sea kelp, sugar, some other
additives, and (of course) MSG.

		 THERE IS NO FUNGI INVOLVED!

	

 Bob (I'm starting feel like Dave Barry) Mulroy

   .



From stamets1@aol.com Wed Dec 28 23:03:12 EST 1994
Article: 1248 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!news-server.ncren.net!taco.cc.ncsu.edu!gatech!howland.reston.ans.net!pipex!uunet!newstf01.news.aol.com!not-for-mail
From: stamets1@aol.com (Stamets 1)
Newsgroups: bionet.mycology
Subject: Re: Heat resistant bacteria???
Date: 27 Dec 1994 19:14:19 -0500
Organization: America Online, Inc. (1-800-827-6364)
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Reply-To: stamets1@aol.com (Stamets 1)



Autoclaves are working fine. The conclusion stands. I have solved the 
problem by switching to better water. Furthermore, I have received 
several e-mail notes concerning this problem and verifying the
extraordinary heat tolerance of these buggers.

thanks for the note, though.

paul


From msachs@ADMIN.OGI.EDU Thu Dec 29 11:39:33 EST 1994
Article: 1249 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!news-server.ncren.net!taco.cc.ncsu.edu!biosci!ADMIN.OGI.EDU!msachs
From: msachs@ADMIN.OGI.EDU (Matthew Sachs)
Newsgroups: bionet.mycology
Subject: Re: Kombucha cultures
Date: 28 Dec 1994 09:16:08 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
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"A Magic Mushroom or a Toxic Fad?" by Molly O'Neill, New York Times p. B1,
28 Dec 94.


-----------------------------------------------------------
Matthew Sachs
Department of Chemistry, Biochemistry and Molecular Biology
Oregon Graduate Institute of Science and Technology
20000 NW Walker Road
P.O. Box 91000
Portland, OR  97291-1000
503 690-1487 Phone
503 690-1464 Fax
msachs@admin.ogi.edu




From stamets1@aol.com Thu Dec 29 11:39:33 EST 1994
Article: 1250 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!news-server.ncren.net!taco.cc.ncsu.edu!gatech!swrinde!howland.reston.ans.net!Germany.EU.net!EU.net!uunet!newstf01.news.aol.com!not-for-mail
From: stamets1@aol.com (Stamets 1)
Newsgroups: bionet.mycology
Subject: further ruminations on bacteria fouling pure culture media
Date: 28 Dec 1994 13:00:26 -0500
Organization: America Online, Inc. (1-800-827-6364)
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Reply-To: stamets1@aol.com (Stamets 1)





Practical Observations on the heat resistance of bacteria: 

My media is pre-mixed prior to sterilization.
 Petri integrity is confirmed. HEPA filter integrity is confirmed.
 I have checked the obvious and not-so-obvious  vectors of contamination.
 I pride myself at being a sleuth in this
 department and my books well illustrate tracking these
 vectors.  Basically what it comes down to is that the more
 contaminants on the front end of the process, the more
 which will survive, even given "sterilization efficiencies of 
99.9999%".  This is easily proved by scooping up some
 garden soil, or manure compost, and making a 20% concoction
 in water, "sterilizing" it for 1-2 hours. In a week, all the plates will
bloom with bacteria. In these situations, I have always double-sterilized
the soil extracts. Even so, I have been surprised by survivors. I wonder
if micro-cavities of organic debris form under pressure which
further increase the probability of endospore survival.
 If one screened endospores in absence of organic debris, 
the results might contrast significantly. Working with substrates 
en masse, which I do on a daily basis (1-2 tons per day at present),
results in insights not seen by technicians doing  "mini-culture". 
I encourage all of you lab-techs working with small batch media
 preparations to visit industrial labs. (This invitation applies to ours.)
 These labs encounter an arena of problems not normally met in
 University and  Research Laboratories. In my mind, "sterilization" is a
 concept, an ideal to which we strive, but one, in practice
 rarely seen in the absolute sense. In effect, pure culture is 
actually a race in predominance of a selected organism against
a universe of background contaminants. The tide of contaminants
is often times too strong, too complex and too diverse to enact
absolute concepts. In chasing the ideal, i.e. exposing the media
to ridiculous extremes of temperature & pressure, the substrate is
often rendered inhospitable in the attempt, and the process
fails. Intelligent compromises more often result in successes. It's
a wonder we can do any pure culture work!

paul stamets


From djdlab@gmm.gen.emory.edu Fri Dec 30 21:15:25 EST 1994
Article: 1251 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!news-feed-1.peachnet.edu!emory!usenet
From: djdlab@bimcore.emory.edu (Dean Danner Lab)
Newsgroups: bionet.mycology
Subject: post doc wanted
Date: 30 Dec 1994 00:13:42 GMT
Organization: Biomolecular Computing Resource, Emory University
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Looking for CALIFORNIA POST DOC...
I will be available for a post doc in the fall '95 and wish to find a lab using fungal systems.  I do need to be in the San Francisco Bay area, Davis, Sacramento, or Santa Barbara.  I have experience in yeast, but would love to branch out.
Does anyone have suggestions?? openings??
Margaret Lanterman
Emory University
mlanterm@gmm.gen.emory.edu





From aj251@FreeNet.Carleton.CA Sat Dec 31 20:09:26 EST 1994
Article: 1252 of bionet.mycology
Newsgroups: bionet.mycology
Path: bigblue.oit.unc.edu!concert!news-server.ncren.net!taco.cc.ncsu.edu!gatech!howland.reston.ans.net!spool.mu.edu!torn!nott!cunews!freenet.carleton.ca!FreeNet.Carleton.CA!aj251
From: aj251@FreeNet.Carleton.CA (Benoit Vincent)
Subject: HOW TO
Message-ID: <D1nG10.9ur@freenet.carleton.ca>
Sender: aj251@freenet3.carleton.ca (Benoit Vincent)
Organization: The National Capital FreeNet, Ottawa, Ontario, Canada
Date: Sat, 31 Dec 1994 00:29:23 GMT
Lines: 8

Is there an easy way to grow mushrooms at home the white kind that
you get at the super market all so my inlaw are Chinese and
love these oyster mushrooms can they be cultivated as well at home
--
To whom I send E-mails
I look in my mail box Wensday mornings and Saturday mornings
at around 8:00 AM ( yes I'm a mornings person )
Thank you for taking the time to reply   B. Vincent.


From seatacdave@aol.com Sat Dec 31 20:09:27 EST 1994
Article: 1253 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!swrinde!pipex!uunet!newstf01.news.aol.com!not-for-mail
From: seatacdave@aol.com (SEATACDAVE)
Newsgroups: bionet.mycology
Subject: Re: POSITION WANTED
Date: 31 Dec 1994 11:12:44 -0500
Organization: America Online, Inc. (1-800-827-6364)
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References: <3d7qv0$o1@newsbf02.news.aol.com>
Reply-To: seatacdave@aol.com (SEATACDAVE)

do you have any experience with button mushroom production?  looking for a
consultant to set up growing operation in asia.  production will be for
export to u.s.a. and europe.  need someone with growing and packing
experience. please respond if interested to:

d. george
fax:  206-670-1225
e-mail  ymbc74a@prodigy.com


From rarnold@teleport.com Sun Jan  1 11:15:31 EST 1995
Article: 1254 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!news-server.ncren.net!taco.cc.ncsu.edu!gatech!howland.reston.ans.net!agate!library.ucla.edu!psgrain!rainrgnews0!news.teleport.com!usenet
From: "Ralph D. Arnold" <rarnold@teleport.com>
Newsgroups: bionet.mycology
Subject: Gyromitra infula culturing??
Date: Sat, 31 Dec 94 13:17:40 PST
Organization: Teleport - Portland's Public Access (503) 220-1016
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I am seeking contacts with any experience growing Gyromitra infula
"Hooded False Morel" , especially/including to fruitbody stage.
Please respond to Ralph Arnold rarnold@teleport.com  Thanks!



From mzieg1234@aol.com Sun Jan  1 20:48:20 EST 1995
Article: 1255 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!howland.reston.ans.net!pipex!uunet!newstf01.news.aol.com!not-for-mail
From: mzieg1234@aol.com (MZieg1234)
Newsgroups: bionet.mycology
Subject: Fruiting of Grifola frondosa
Date: 1 Jan 1995 18:41:53 -0500
Organization: America Online, Inc. (1-800-827-6364)
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Reply-To: mzieg1234@aol.com (MZieg1234)

Fruiting Gf indoors has eluded me. Outdoors I have had sawdust beds
produce but no results indoors.  My current conditions produce good spawn
run and very occasional primordia: Oak sawdust with maple chips, 5% wheat
bran, 1% gypsum. Grain spawn added at 10%.  30, 45, 60 day spawn run at 72
deg. I then drop the temperature to 62 deg and either open the bag
narrowly at top or fold over and express all gasses after making two 0.5
cm holes on the side of the bag to create a CO2 gradient.  These
conditions have not worked with 3 of my own isolates or 1 commercial
"fruiting variety."  The above conditions work well in my hands with a
number of other species including Lions mane, Black poplar, Elm oyster,
King oyster. Is there a trick I am missing?  I know I am not alone with
this recalcitrant (but nice!) species.  Thank you-Mike Ziegler
MZieg1234@aol.com


From mayer@osiris.cso.uiuc.edu Mon Jan  2 23:32:40 EST 1995
Article: 1256 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!howland.reston.ans.net!vixen.cso.uiuc.edu!osiris.cso.uiuc.edu!mayer
From: mayer@osiris.cso.uiuc.edu (James Mayer)
Newsgroups: bionet.mycology
Subject: Mycelium I.D.
Date: 2 Jan 1995 15:01:40 GMT
Organization: University of Illinois at Urbana
Lines: 19
Message-ID: <3e94gk$c69@vixen.cso.uiuc.edu>
NNTP-Posting-Host: osiris.cso.uiuc.edu



My first attempt at germinating spores.  Problem: Identifying if what in
fact is growing is mycelium - something is growing.  I previously identified
contamination, but this time I'm not sure.  I'm using "The Mushroom
Cultivator - Stamets" as my guide, but with little/no experience I can't be
sure. I'm getting good radial growth, cottony with no rhizomorphic growth,
all white color.  With no rhizomorphic growth I can't be sure. Is there a
good/better I.D. technique?

Are there any local (Washington D.C.) growers that might be willing to help
I.D. my growth? 

Thanks,

Jim.





From bknack@uoguelph.ca Mon Jan  2 23:32:41 EST 1995
Article: 1257 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!bloom-beacon.mit.edu!spool.mu.edu!torn!nermal.cs.uoguelph.ca!gadwall.cs.uoguelph.ca!bknack
From: bknack@uoguelph.ca (Bruce Knack)
Newsgroups: bionet.mycology
Subject: Any good Mold ID Books?
Date: 2 Jan 1995 16:57:43 GMT
Organization: University of Guelph
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Hi Everyone:

I'm posting this for a friend who would like to know of any good books 
you might be using to help identify molds.  She's primarily interested in 
human-pathogenic molds, but anything would help.

TIA,
Bruce.



From bmrarick@aol.com Mon Jan  2 23:32:42 EST 1995
Article: 1258 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!news.duke.edu!agate!howland.reston.ans.net!pipex!uunet!newstf01.news.aol.com!not-for-mail
From: bmrarick@aol.com (BMRARICK)
Newsgroups: bionet.mycology
Subject: to:all
Date: 2 Jan 1995 14:08:42 -0500
Organization: America Online, Inc. (1-800-827-6364)
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Reply-To: bmrarick@aol.com (BMRARICK)

Are there any N.A.M.A. members in this newsgroup?


From ptm@MOLBIO.CBS.UMN.EDU Mon Jan  2 23:32:42 EST 1995
Article: 1259 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!howland.reston.ans.net!swrinde!sgiblab!sgigate.sgi.com!olivea!biosci!MOLBIO.CBS.UMN.EDU!ptm
From: ptm@MOLBIO.CBS.UMN.EDU (Paul T. Magee)
Newsgroups: bionet.mycology
Subject: heat resistant bacteria
Date: 2 Jan 1995 11:31:31 -0800
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The phenomenon of bacteria surviving a standard autoclaving is neither new nor
unique.  What's happening is that the spores are either not getting to 120 or
there are so many that a few survive.  The best way to deal with this problem
is Tyndallization, which is to autoclave twice, with a couple of hours between
the two treatments.  Heat causes the spores to germinate, and then they become
susceptible to standard sterilization techniques.
Pete Magee


From ad727@rgfn.epcc.Edu Tue Jan  3 22:16:33 EST 1995
Article: 1260 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!news-server.ncren.net!taco.cc.ncsu.edu!biosci!RGFN.EPCC.EDU!ad727
From: ad727@RGFN.EPCC.EDU (Lester Pasarell)
Newsgroups: bionet.mycology
Subject: Re: Any good Mold ID Books?
Date: 2 Jan 1995 15:01:31 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
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>
>Hi Everyone:
>
>I'm posting this for a friend who would like to know of any good books 
>you might be using to help identify molds.  She's primarily interested in 
>human-pathogenic molds, but anything would help.
>
>TIA,
>Bruce.
>
>
In response to your query, there are several good books, some general some
specific about identification of the moulds that could be encountered in
the clinical laboratory for example:

General
Mcginnis, M.R 1980 Laboratory Handbook of medical Mycology, Academic Press

Carmichael, J.W et al 1980 Genera of Hyphomycetes> The University of Alberta
Press

Domsch, K.H., W. Gams, and T. Anderson 1980 Compendium of Soil Fungi

Stevens, R. B. ed 1974. Mycology Guidebook, Univ of Washington Press, Seattle

For Dermatophytes I like to use Rebell, G and D. Taplin 1964. Dermatophytes:
Their recognition and identification. Univ of Miami Press

For Dematiaceous Hyphomycetes I like to use : Ellis, M.B. 1971 Dematiaceous
Hyphomycetes . Commonwealth Mycological Institute, Kew

And there are many more that you could use in the clinical laboratory
for proper identification of an isolate. If you are interest send me a E-Mail
with your mailing address and I will send you a list of some other books
that we use here. 
All the ones listed above could give you a good start, and I bet out there
someone could give the rest of us in the mycology.bionet group their list
of favorite books for identification of hyphomycetes.

Lester Pasarell(ad727@rgfn.epcc.edu)
Medical Mycology Research laboratory
University of Texas Medical Branch
Galveston, Texas



>


From mspear@telerama.lm.com Tue Jan  3 22:16:34 EST 1995
Article: 1261 of bionet.mycology
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From: mspear@telerama.lm.com (mspear)
Newsgroups: bionet.mycology
Subject: Bacteria Survival
Date: 2 Jan 1995 19:02:57 -0500
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I've got to go with Pete.

We sterilize something like 40 million pounds of substrate each year. 
For evolution to be a reasonable explanation you would have to be
re-autoclaving your substrate to provide a selection step.

Very commonly, however, we see high initial contamination loads lead
to failed sterilizations.  Look up heat kill curves.  It's a
probalistic process that only asymptoticly approaches complete kill.

Sudden and recent evolution is too easy an explanation.  In our
experience there is always a deeper and simpler explanation.


From rhalling@nybg.org Tue Jan  3 22:16:36 EST 1995
Article: 1262 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!howland.reston.ans.net!agate!biosci!nybg.org!rhalling
From: rhalling@nybg.org
Newsgroups: bionet.mycology
Subject: Re: Any good Mold ID Books?
Date: 3 Jan 1995 06:32:45 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
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I thought the following information might be useful to mycologists looking for
mycological literature.

Roy Halling               rhalling@nybg.org
New York Botanical Garden Bronx, NY 10458


CATALPA, Online Catalog of the Library of The New York
         Botanical Garden, Accessible on the Internet

CATALPA, the online catalog of the Library of The New York 
Botanical Garden is now accessible through the Internet.  The
Library's telnet address is librisc.nybg.org  or 192.77.202.200.

CATALPA is an acronym for CATAlog for Library Public Access; (it
is also a small genus of American and Asiatic trees of the
trumpet-creeper family--Bignoniaceae).  CATALPA is fully
searchable using traditional card catalog approaches by author,
title, or subject.  It also provides search by call number or key
words in title, corporate entry, or contents notes, and allows
browsing through the collection in call number or "shelf list"
order, a virtual walk through the stacks.  These searching
strategies greatly facilitate bibliographic access to the
holdings of the NYBG Library and to the literature of the plant
sciences.

The NYBG Library collection numbers over 250,000 volumes (almost
110,000 titles of books and journals) in botany, horticulture,
landscape design and related fields.  Its particular strengths
are: plant systematics, floristics, plant ecology, the history of
botany, horticulture and gardening, biography, and garden and
landscape design.

For additional information about CATALPA and the Library of The
New York Botanical Garden contact John F. Reed, Director of the
Library, The New York Botanical Garden, Bronx, NY  10458-5126; 
or jfreed@nybg.org.  




.



From mzieg1234@aol.com Tue Jan  3 22:16:38 EST 1995
Article: 1263 of bionet.mycology
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From: mzieg1234@aol.com (MZieg1234)
Newsgroups: bionet.mycology
Subject: Bacteria fouling pure culture comment
Date: 2 Jan 1995 21:41:33 -0500
Organization: America Online, Inc. (1-800-827-6364)
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Reply-To: mzieg1234@aol.com (MZieg1234)

Paul questioned in his note of 12/24 about gene migration and antibiotic
resistance. There is an enormous literature on this subject. There are at
least three types of transfer. Transformation is the direct transfer of
chemical genetic information (DNA or RNA). Transduction is the transfer of
information (drug resistance or other characteristics) by the vector of
bacterial virus. A third mechanism is R-factor transfer which is more
complicated but can involve mating of dissimilar genera through
facilitation by genetic factors. The above topics form the basis of much
of modern molecular biology (for a delightful account of the people
involved in this "golden age" of molecular biology see Eight Days of
Creation by Horace Judson). I am concerned that many cultivators promote
the routine use of gentimicin.  Resistant bacterial strains of all sorts
will eventually be selected (including human pathogens). Cross resistance
to other antibiotics is common and might make these pathogens difficult to
treat.  If I have bacterial contamination I autoclave and discard the
critters. This is painful to do with valuable cultures but... We all know
that the best cure is attention to detail and prevention.  Mike Ziegler


From csmith@gulf.net Tue Jan  3 22:16:39 EST 1995
Article: 1264 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!news-server.ncren.net!taco.cc.ncsu.edu!gatech!news-feed-1.peachnet.edu!usenet.eel.ufl.edu!cobia.gulf.net!usenet
From: Chris Smith <csmith@gulf.net>
Newsgroups: bionet.mycology
Subject: Re: Any good Mold ID Books?
Date: 3 Jan 1995 02:51:18 GMT
Organization: The Gulf Coast Internet Company
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NNTP-Posting-Host: slip10.gulf.net

ad727@RGFN.EPCC.EDU (Lester Pasarell) wrote:
>
> 
> 
> >
> >Hi Everyone:
> >
> I have found for clinical identification of yeast and molds the 
following book to be most helpful.
Larone, Davise H. - Medically Important Fungi - A Guide to Identification
Nancy Norris
TB/Mycology
West Florida Regional Medical Center



From an524@FreeNet.Carleton.CA Tue Jan  3 22:16:40 EST 1995
Article: 1265 of bionet.mycology
Newsgroups: bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!swrinde!sgiblab!spool.mu.edu!torn!nott!cunews!freenet.carleton.ca!FreeNet.Carleton.CA!an524
From: an524@FreeNet.Carleton.CA (Jeffrey Ehlinger)
Subject: Re: HOW TO
Message-ID: <D1tEzL.L1s@freenet.carleton.ca>
Sender: an524@freenet.carleton.ca (Jeffrey Ehlinger)
Organization: The National Capital FreeNet, Ottawa, Ontario, Canada
Date: Tue, 3 Jan 1995 05:52:33 GMT
Lines: 4

	The one you refer to is I believe,
Agaricus Campestrus (var. bisporus)
	Did I spell that correctly?
-Jeff


From heytler@strauss.udel.edu Tue Jan  3 22:16:41 EST 1995
Article: 1266 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!udel!news-4.nss.udel.edu!strauss.udel.edu!not-for-mail
From: heytler@strauss.udel.edu (Peter Heytler)
Newsgroups: bionet.mycology
Subject: Re: to:all
Date: 3 Jan 1995 21:14:30 -0500
Organization: University of Delaware
Lines: 8
Message-ID: <3ed0a6$1gu@strauss.udel.edu>
References: <0098937A.A0EAB811.30@genome.genetics.uga.edu> <3e9ivq$jh6@newsbf02.news.aol.com>
NNTP-Posting-Host: strauss.udel.edu

In article <3e9ivq$jh6@newsbf02.news.aol.com>,
BMRARICK <bmrarick@aol.com> wrote:
>Are there any N.A.M.A. members in this newsgroup?

I'm sure there are. I have been a member for a good many years, though 
not particularly active.
--Peter Heytler



From nimra@im.uib.no Wed Jan  4 20:12:10 EST 1995
Article: 1267 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!gt-news!cc.gatech.edu!darwin.sura.net!lhc!biosci!agate!howland.reston.ans.net!pipex!sunic!trane.uninett.no!due.uninett.no!alf.uib.no!pc36.im.uib.no!nimra
From: nimra@im.uib.no (Roald Aasen)
Newsgroups: bionet.mycology
Subject: Re: Any good Mold ID Books?
Date: Wed, 4 Jan 1995 11:13:59
Organization: Unversity of Bergen
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In article <3e9ba7$l6l@nermal.cs.uoguelph.ca> bknack@uoguelph.ca (Bruce Knack) writes:
>From: bknack@uoguelph.ca (Bruce Knack)
>Subject: Any good Mold ID Books?
>Date: 2 Jan 1995 16:57:43 GMT

>Hi Everyone:

>I'm posting this for a friend who would like to know of any good books 
>you might be using to help identify molds.  She's primarily interested in 
>human-pathogenic molds, but anything would help.

>TIA,
>Bruce.

I recommend McGinnis.M.R.: Laboratory Handbook of Medical Mycology, 1980, 
Academic Press, 660 pages.



From steriti@cpe.uml.edu Wed Jan  4 20:12:11 EST 1995
Article: 1268 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!howland.reston.ans.net!europa.eng.gtefsd.com!ulowell.uml.edu!cpe.uml.edu!steriti
From: steriti@cpe.uml.edu (Ron Steriti)
Newsgroups: bionet.mycology
Subject: shiitake growing (beginner questions)
Date: 4 Jan 1995 16:38:02 GMT
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The block of shiitakes i got from Fungi Perfecti is producing it's
first flush, and i'm wondering what's the best (easiest, low cost)
method of growing some more.

The best method appears to be to use high temp bags,
the ones from F.P. appear to be fairly expensive,
are there cheaper sources?

Is it possible to use mason jars, or is the mushroom too big?

Thanks for any/all help

ron



-- 
Dr. Ronald Steriti    steriti@cpe.uml.edu   
		      http://dragon.cpe.uml.edu/~steriti/Interesting.html

Living has taught me how to think, but thinking has not taught me how to live.
   -Herzen


From rarnold@teleport.com Wed Jan  4 20:12:12 EST 1995
Article: 1269 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!bloom-beacon.mit.edu!uhog.mit.edu!europa.eng.gtefsd.com!newsxfer.itd.umich.edu!nntp.cs.ubc.ca!psgrain!rainrgnews0!news.teleport.com!usenet
From: "Ralph D. Arnold" <rarnold@teleport.com>
Newsgroups: bionet.mycology
Subject: Re: shiitake growing (beginner questions)
Date: Wed, 4 Jan 95 17:07:57 PST
Organization: Teleport - Portland's Public Access (503) 220-1016
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To: steriti@cpe.uml.edu

Have you considered subscribing to FUNGUS, the mailing list for mushroom
cultivation?  These and many other issues are discussed there.
Subscribe by sending message to fungus-request@teleport.com with
message SUBSCRIBE FUNGUS <you@your.domain>

Archived messages are available via anonymous FTP at ftp.teleport.com
under directories \pub\users\rarnold\mushroom\FUNGUS

Good luck!  Ralph D. Arnold, rarnold@teleport.com

On 4 Jan 1995 16:38:02 GMT, 
Ron Steriti  <steriti@cpe.uml.edu> wrote:

>
>The block of shiitakes i got from Fungi Perfecti is producing it's
>first flush, and i'm wondering what's the best (easiest, low cost)
>method of growing some more.
>
>The best method appears to be to use high temp bags,
>the ones from F.P. appear to be fairly expensive,
>are there cheaper sources?
>
>Is it possible to use mason jars, or is the mushroom too big?
>
>Thanks for any/all help
>



From ad727@rgfn.epcc.Edu Thu Jan  5 21:04:03 EST 1995
Article: 1270 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!bloom-beacon.mit.edu!spool.mu.edu!olivea!biosci!RGFN.EPCC.EDU!ad727
From: ad727@RGFN.EPCC.EDU (Lester Pasarell)
Newsgroups: bionet.mycology
Subject: CAB Mycology Abstracts
Date: 4 Jan 1995 21:59:33 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
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NNTP-Posting-Host: net.bio.net



Hi there! Does anyone knows if the CAB Veterinary and Medical Mycology
abstracts, are offered on line by any Library or Service?, Do they charge?
Thanks
Lester Pasarell


From griffin@rodan.syr.edu Thu Jan  5 21:04:04 EST 1995
Article: 1271 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!newsfeed.pitt.edu!uunet!news.unr.edu!ub!galileo.cc.rochester.edu!newstand.syr.edu!rodan.syr.edu!griffin
From: griffin@rodan.syr.edu (David H. Griffin)
Newsgroups: bionet.mycology
Subject: Expression of (fungus) gene on different loci ?
Date: 5 Jan 1995 21:02:27 GMT
Organization: Syracuse University, Syracuse NY
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Wolf Lichtenberger wrote:

     I'm sure that a gene varies it's protein production rate if situated
   on different loci. Any experiences in the field of gene expression
   variation at the transcription level would be welcome, especially with
   fungi (filamentous fungi would be even better :-) )

Timberlake and Hamer discuss position effects on expression of genes in 
the SpoC1 cluster of Aspergillus nidulans in their review:

Timberlake, W. E. and J. E. Hamer.  1986.  Regulation of gene activity 
during conidiophore development in Aspergillus nidulans.  Genetic 
Engineering 8: 1-29.

                                 
                                  
David H. Griffin               
College of Environmental         
    Science and Forestry        
Syracuse NY 13210-2788         
e-mail: griffin@mailbox.syr.edu
  



From jdkirkla@prairienet.org Thu Jan  5 21:45:13 EST 1995
Article: 1272 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!howland.reston.ans.net!vixen.cso.uiuc.edu!prairienet.org!jdkirkla
From: jdkirkla@prairienet.org (Justin D. Kirkland)
Newsgroups: bionet.mycology
Subject: Poria cocos
Date: 6 Jan 1995 01:56:23 GMT
Organization: University of Illinois at Urbana
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NNTP-Posting-Host: firefly.prairienet.org


I am writing in asking for help on cultivating Poria cocos.
I have ATCC strain #42588 which states that it fruits in 
culture.  So far I have not been able to get any fruitings.
The Japanese Journal of Pharmacology had an article on the
formation of fruitbodies of Poria cocos in the March of 1994
issue.  However all my attempts at locating this journal have
been unsuccessful.  I am doing this as part of a research
project at the University of Illinois so I won't be making
any money off of it if that is anyones concern.
Thanks in advance,
Justin Kirkland
-- 


From microbe@CSOS.ORST.EDU Sat Jan  7 11:12:05 EST 1995
Article: 1273 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!news-feed-1.peachnet.edu!insosf1.infonet.net!usenet.ee.pdx.edu!news.reed.edu!gaia.ucs.orst.edu!news.CSOS.ORST.EDU!microbe
From: microbe@CSOS.ORST.EDU (Steven Carpenter)
Newsgroups: bionet.mycology
Subject: Jurassic Pine Fungi
Date: 30 Dec 1994 19:23:23 GMT
Organization: CS Outreach Services, Oregon State University, Corvallis, OR, USA
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Is anyone out there planning to attempt a study of the fungi associated
with the new find of relict Araucarioids in Australia?

I would imagine that among numerous organisms associated with the
litter, that the fungi which are parasitic, saprophytic and mycorrhizal
with these trees would be potential targets of research.

In addition, one might be able to test hypotheses concerning evolution
of organisms that have adapted to single hosts over time.  There may
or may not be a component of the ancient fungal population that has
survived with the tree.  There may also be common fungi which have
adapted to decomposition of tree parts which have crowded out any
ancient fungi.  And there may be some kind of mix of both.

What a rich find for all biologists!

-Steve Carpenter
 Abbey Lane Laboratories
 microbe@csos.orst.edu




From crta@xnet.com Sat Jan  7 11:12:06 EST 1995
Article: 1274 of bionet.mycology
Newsgroups: bionet.molbio.methds-reagnts,bionet.molbio.proteins,bionet.molbio.rapd,bionet.molbio.yeast,bionet.mycology,bionet.n2-fixation,bionet.neuroscience,bionet.photosynthesis,bionet.plants,bionet.population-bio,bionet.software,bionet.software.acedb,bionet.software.gcg,bionet.users.addresses,bionet.virology,bionet.women-in-bio,bionet.xtallography,biz.americast,biz.books.technical,biz.comp.hardware
Path: bigblue.oit.unc.edu!concert!news-server.ncren.net!taco.cc.ncsu.edu!gatech!newsfeed.pitt.edu!uunet!xnet!quake.xnet.com!research
From: crta@xnet.com (Norman Fraley)
Subject: New Research & Testing Association Formed
Message-ID: <D1yCnp.2G6@amiserv.chi.il.us>
Sender: news@amiserv.chi.il.us
Nntp-Posting-Host: research.crta.org
Organization: Contract Research & Testing Association
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Date: Thu, 5 Jan 1995 20:53:53 GMT
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Xref: bigblue.oit.unc.edu bionet.molbio.methds-reagnts:21306 bionet.molbio.proteins:3168 bionet.molbio.rapd:876 bionet.molbio.yeast:1952 bionet.mycology:1274 bionet.n2-fixation:11 bionet.neuroscience:5279 bionet.photosynthesis:493 bionet.plants:4598 bionet.population-bio:938 bionet.software:9873 bionet.software.acedb:462 bionet.software.gcg:828 bionet.users.addresses:2007 bionet.virology:1240 bionet.women-in-bio:1555 bionet.xtallography:1307 biz.books.technical:1021 biz.comp.hardware:8271

As the primary resource of research information, the Internet was the
primary choice for making all concerned individuals aware of the formation
of the Contract Research & Testing Association.

CRTA is an International Association designed to serve the needs of contract
research, product and process development organizations and consultants
throughout the world.  Contract research organizations have specific public,
governmental, and industry perception and promotion needs which are not addressed
by existing scientific industry associations.  CRTA operates as a non-profit,
tax-exempt, corporation eligible for scientific research and public awareness
charitable organization contributions as provided for in the IRC 501(c)(3) provisions.

Being a scientific research and public awareness related organization, CRTA
exists to benefit its members by providing:

  1) An organization devoted to the promotion of Contract Research.
  2) A unified voice on matters of common interest or concern.
  3) Point of contact for media relations relative to contract research.
  4) Business opportunity referrals as a research clearinghouse.
  5) Professional networking opportunities for its members.
  6) Periodic publishing of information beneficial to the membership.
  7) Periodic dissemination of applicable research results to the public.
  8) Governmental representation on issues affecting CRO's.
  9) Public promotion of the strengths of its membership.
 10) A directory of Contract Research Organizations and Consultants.

CRTA will provide:
  1)  A forum for the exchange of information.
  2)  Formal recognition to the CRO's role in business.
  3)  Standards for the professionals so engaged.
  4)  Representation the profession in matters of common interest.
  5)  The development of techniques and methods to improve the practice and
      management of CROs.

CRTA will also offer:
  1)  A monthly news publication.
  2)  Annual meetings
  3)  Active promotional media publicity programs.
  4)  A professional placement service
  5)  A Contract Research Service Directory.
  6)  Media topics and contacts directory

If you have an interest in joining the Contract Research & Testing Association,
please E-mail your reply to crta@xnet.com.  Please include:

1) The word "membership" in your RE: or header information,
2) Your interest in the association / your area of work,
3) Your dues payment preference (check, money order, credit card, company check, wire xfer, etc.)
   DO NOT INCLUDE ANY CREDIT CARD INFORMATION!  Only your preference for the manner of payment.
4) Most importantly, your email address, and additional contact information if you desire.

We will then e-mail membership information and ALL FURTHER INFORMATION
directly to you at your email location.  Thank you for taking the time
to read this announcement.  If membership in this program this does not
appeal to you, thank you for your patience and understanding.

Sincerely,
Membership Department
Contract Research & Testing Association


Best Regards,

Norman Fraley                                         CRTA@xnet.com
Executive Director                                   BBS:708-515-0494
Contract Research & Testing Association


From EVERSL@AXE.HUMBOLDT.EDU Sat Jan  7 11:12:06 EST 1995
Article: 1275 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!howland.reston.ans.net!agate!biosci!AXE.HUMBOLDT.EDU!EVERSL
From: EVERSL@AXE.HUMBOLDT.EDU
Newsgroups: bionet.mycology
Subject: request for info on key-writing
Date: 5 Jan 1995 21:10:06 -0800
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Can anyone direct me to a source for software useful for writing a 
dichotomous key?  I feel that I've done my time on Word Perfect and assume
there must be a better way...:}

Linda Evers
eversl@axe.humboldt.edu

Thanks 


From ingber@alumni.caltech.edu Sat Jan  7 11:12:07 EST 1995
Article: 1276 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!howland.reston.ans.net!news.cac.psu.edu!news.pop.psu.edu!hudson.lm.com!netline-fddi.jpl.nasa.gov!nntp-server.caltech.edu!ingber
From: ingber@alumni.caltech.edu (Lester Ingber)
Newsgroups: bionet.molbio.methds-reagnts,bionet.molbio.proteins,bionet.molbio.rapd,bionet.molbio.yeast,bionet.mycology,bionet.n2-fixation,bionet.neuroscience,bionet.photosynthesis,bionet.plants,bionet.population-bio,bionet.software,bionet.software.acedb,bionet.software.gcg,bionet.users.addresses,bionet.virology,bionet.women-in-bio,bionet.xtallography,biz.americast,biz.books.technical,biz.comp.hardware
Subject: Re: New Research & Testing Association Formed
Date: 6 Jan 1995 14:04:51 GMT
Organization: California Institute of Technology, Alumni Association
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Norman:
 
I like the concept of what you are trying to form, and so I would like
to explain why I will not join now, but might be interested at some
future time.  My criticism is meant to be constructive, and I hope it
will be accepted in this context.
 
Since I think your organization is a great idea, I'm making my response
public so that other researchers who might be turned off by some of
these problems in your presentation also will keep an open mind to
joining your organization in the future.  (You really did span quite
few  bulletin boards!)
 
: From crta@xnet.com Thu Jan  5 21:47:00 1995
: Return-Path: <crta@xnet.com>
: Date: Thu, 5 Jan 1995 23:46:55 -0600
: From: Norman Fraley <crta@xnet.com>
: To: ingber@alumni.caltech.edu
: Subject: Membership  information for CRTA
:
: Dear Mr. Ingber
:
: WHAT IS CRTA?
:
: CRTA, formally known as the Contract Research and Testing Association, is
: an
: international scientific organization whose primary objective is to build
: awareness of the role of contract research in industry and in society,
: represent the industry in matters of common interest and to provide a forum
: for the exchange of ideas and techniques in the fields of Testing and
: Applied
: Research.
 
If you are appealing to a large audience over the InterNet, you should
follow some commonly accepted guidelines, e.g., delivering text in a
most readable format, e.g., limiting lines to 79 characters, so that
lines do not spill over on most 80-character screens.
 
This is the most vital context of my critique, that you will not come
across as a knowledgeable and professional organization unless you
present yourself as such.
 
For example, I put your 424-line e-mail reply to my follow-up to this
posting through ispell, and here is a partial list which also does not
register in `webster`:
        copywrite
        lnternational
        nonanalytical
        nonmicrobiology
        proovided
        spectroscopists
        toxicologists
 
: CRTA is known for the efforts it has made in helping to promote the new and
: rapidly growing industry of contract research.  This is accomplished by a
: network of nearly 700 commercial, industry, government, and academic
: laboratories and individual product and process development consultants.
: These promotional activities and other CRTA programs offer you, the
: technical
: professional, avenues for professional growth and recognition that only an
: organization of CRTA's caliber can provide ... plus access to a wellspring
: of
: analytical, research and technical information.
 
I question whether you already have such a membership, or this is what
you aspire to?  This is a reasonable question.  Many people, like
myself, are more sympathetic to an honest statement from a new
operation, than to misleading advertisements.
 
I note a number of journals and publications you (intend to?) make
available, and I expect they have a price.  Your fees for members of
$125/yr is fair, if all these services are available now.  All this is
fine, but those prices should be stated up front.
 
: Email:  crta@xnet.com
: WWW:  www.xnet.com/~crta
 
I tried this site, http://www.xnet.com/~crta, and found a short notice
that it is under construction.  I think it better to first do your
homework, before advertising such a site, especially since you really
are charging commercial rates for a commercial venture, your non-profit
status notwithstanding.
 
: copywrite 1994 Contract Research & Testing Association.
 
This one is a real killer, demonstrating that you are not sensitive to
one of the main concerns of contract research, the copyright (not the
"copywrite").
 
Lester

In article <D1yCnp.2G6@amiserv.chi.il.us>, Norman Fraley <crta@xnet.com> wrote:
:As the primary resource of research information, the Internet was the
:primary choice for making all concerned individuals aware of the formation
:of the Contract Research & Testing Association.
:
:CRTA is an International Association designed to serve the needs of contract
:research, product and process development organizations and consultants
:throughout the world.  Contract research organizations have specific public,
:governmental, and industry perception and promotion needs which are not addressed
:by existing scientific industry associations.  CRTA operates as a non-profit,
:tax-exempt, corporation eligible for scientific research and public awareness
:charitable organization contributions as provided for in the IRC 501(c)(3) provisions.
:
:Being a scientific research and public awareness related organization, CRTA
:exists to benefit its members by providing:
:
:  1) An organization devoted to the promotion of Contract Research.
:  2) A unified voice on matters of common interest or concern.
:  3) Point of contact for media relations relative to contract research.
:  4) Business opportunity referrals as a research clearinghouse.
:  5) Professional networking opportunities for its members.
:  6) Periodic publishing of information beneficial to the membership.
:  7) Periodic dissemination of applicable research results to the public.
:  8) Governmental representation on issues affecting CRO's.
:  9) Public promotion of the strengths of its membership.
: 10) A directory of Contract Research Organizations and Consultants.
:
:CRTA will provide:
:  1)  A forum for the exchange of information.
:  2)  Formal recognition to the CRO's role in business.
:  3)  Standards for the professionals so engaged.
:  4)  Representation the profession in matters of common interest.
:  5)  The development of techniques and methods to improve the practice and
:      management of CROs.
:
:CRTA will also offer:
:  1)  A monthly news publication.
:  2)  Annual meetings
:  3)  Active promotional media publicity programs.
:  4)  A professional placement service
:  5)  A Contract Research Service Directory.
:  6)  Media topics and contacts directory
:
:If you have an interest in joining the Contract Research & Testing Association,
:please E-mail your reply to crta@xnet.com.  Please include:
:
:1) The word "membership" in your RE: or header information,
:2) Your interest in the association / your area of work,
:3) Your dues payment preference (check, money order, credit card, company check, wire xfer, etc.)
:   DO NOT INCLUDE ANY CREDIT CARD INFORMATION!  Only your preference for the manner of payment.
:4) Most importantly, your email address, and additional contact information if you desire.
:
:We will then e-mail membership information and ALL FURTHER INFORMATION
:directly to you at your email location.  Thank you for taking the time
:to read this announcement.  If membership in this program this does not
:appeal to you, thank you for your patience and understanding.
:
:Sincerely,
:Membership Department
:Contract Research & Testing Association
:
:
:Best Regards,
:
:Norman Fraley                                         CRTA@xnet.com
:Executive Director                                   BBS:708-515-0494
:Contract Research & Testing Association


-- 
/* Prof. Lester Ingber                                                * 
 * Lester Ingber Research           E-Mail: ingber@alumni.caltech.edu * 
 * P.O. Box 857               WWW: http://alumni.caltech.edu/~ingber/ * 
 * McLean, VA 22101      Archive: ftp.alumni.caltech.edu:/pub/ingber/ */


From griffin@mail.utexas.edu Sat Jan  7 21:52:10 EST 1995
Article: 1277 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!swrinde!cs.utexas.edu!geraldo.cc.utexas.edu!usenet
From: griffin@mail.utexas.edu (ARGONAUT)
Newsgroups: bionet.mycology
Subject: Spore Growth Questions...
Date: 7 Jan 1995 19:51:46 GMT
Organization: The University of Texas at Austin
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I have 2 questions.

first, can spores be taken from dried mushrooms, and later grown?
does anyone have any experience with this?  i suspect that
they can be taken from dried mushrooms, since the prints themselves
dry on their own.

second, what is the needed sterilitity of the spore print to grow?
i dont understand how the print must be sterile, since the specimen
it was taken from was not sterile, it was grown in a field or whatever.
this isnt to underestimate the need for sterility in the growth, but must 
the spore print be sterile?


thanks for the help 


From boe666@u.washington.edu Sun Jan  8 22:59:55 EST 1995
Article: 1278 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!howland.reston.ans.net!spool.mu.edu!news.clark.edu!netnews.nwnet.net!news.u.washington.edu!boe666
From: boe666@u.washington.edu (Peter Werner)
Newsgroups: bionet.mycology,sci.bio.ecology
Subject: Climate change and mycorrhizae
Date: 8 Jan 1995 00:31:09 GMT
Organization: University of Washington
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Originator: boe666@homer19.u.washington.edu
Xref: bigblue.oit.unc.edu bionet.mycology:1278 sci.bio.ecology:8106

I'm going to be writing a research paper on the effects of several types 
of environmental disruption on mycorrhizae and how this in turn effects 
forest health.  So far, I've been able to find quite a bit of literature 
on the effects of acid precipitation on mycorrhizae, as well as some 
literature on the effects of disturbances such as logging.

What I haven't been able to find is anything about the effects of climate 
change on mycorrhizal fungi.  Changes in temperature and, especially, 
precipitation patterns would seem to have a strong effect on these fungi, 
significantly altering the distribution of these species, at the very 
least.  If anyone knows of any literature that has been written about
this subject, I'd greatly appreciate the reference.

Thanks in advance,
PGW


From todell@u.washington.edu Sun Jan  8 22:59:56 EST 1995
Article: 1279 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!howland.reston.ans.net!vixen.cso.uiuc.edu!uwm.edu!reuter.cse.ogi.edu!netnews.nwnet.net!news.u.washington.edu!homer01.u.washington.edu!todell
From: Thomas O'Dell <todell@u.washington.edu>
Newsgroups: bionet.mycology,sci.bio.ecology
Subject: Re: Climate change and mycorrhizae
Date: Sun, 8 Jan 1995 19:42:54 -0800
Organization: University of Washington
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Xref: bigblue.oit.unc.edu bionet.mycology:1279 sci.bio.ecology:8113

i have seen papers on effects of elevated CO2 on ectomycorrhizae, also 
temperature effects. sorry but i dont have references handy. try science 
citation most recent 5 year index and search subject ectomycorrhizaethen 
look for CO2...
The other global change to be aware of (and more certain than temperature 
changes in terms of magnitude and direction) is in N budgets something 
like a 100% increase in terrestrial N inputs over the past 100 years, 
mostly during the past 10. (see Vitousek, P. 1994. Beyond Global 
Warming: Ecology and Global Change). 
75: 1861) Have fun, and get back to me if you hit a dead end.


From larsont@rnisd0.DNET.roche.com Mon Jan  9 21:32:13 EST 1995
Article: 1280 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!howland.reston.ans.net!agate!biosci!rnisd0.DNET.roche.com!larsont
From: larsont@rnisd0.DNET.roche.com
Newsgroups: bionet.mycology
Subject: Abstract, Larson and Nuss, EMBO J.  13(1994), 5616-5623.
Date: 9 Jan 1995 07:09:32 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
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For reprints send snail mail address to: "larsont@rnisd0.DNET.roche.com".


Altered Transcriptional Response to Nutrient Availability in Hypovirus-Infected
Chestnut Blight Fungus

EMBO J.  13(1994), 5616-5623

Thomas G. Larson and Donald L. Nuss

Roche Institute of Molecular Biology
Roche Research Center
Nutley, NJ 07110, USA


Abstract

The gene lac-1, encoding the enzyme laccase, is one of several genes 
of the chestnut blight fungus, Cryphonectria parasitica, that are suppressed 
by virulence-attenuating mycoviruses of the genus Hypovirus.  Two 
antagonistic regulatory pathways have been shown to govern the activity 
of the lac-1 promoter: a positive pathway that stimulates transcription 
and a negative pathway that represses transcription.  We now report that
these two regulatory pathways respond independently to specific changes 
in the nutritional environment.  These newly defined conditions were used
to confirm that the hypovirus suppresses the activity of the positive
regulatory pathway and to implicate calmodulin and calcineurin as components
of the signal transduction cascades regulating lac-1 transcription.
Significantly, lac-1 transcript accumulation was shown to be affected 
by amino acid availability.  Further analysis revealed that transcriptional
repression mediated by the negative regulatory pathway is relieved under
conditions of amino acid deprivation.  Thus, by blocking the positive pathway,
hypovirus infection prevents increased lac-1 transcript accumulation in
response to amino acid deficiency.  These observations are consistent with 
the hypothesis that hypoviruses alter the transcriptional response of the 
host fungus to changes in nutritional availability.  



From Sergi.Ferrer@uv.es Mon Jan  9 21:32:14 EST 1995
Article: 1281 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!howland.reston.ans.net!pipex!news.oleane.net!oleane!jussieu.fr!univ-lyon1.fr!swidir.switch.ch!scsing.switch.ch!news.rediris.es!power.ci.uv.es!ginjol.microb.uv.es!Sergi.Ferrer
From: Sergi.Ferrer@uv.es (Sergi Ferrer)
Newsgroups: bionet.mycology
Subject: Book references
Date: Mon, 9 Jan 1995 16:30:55 GMT
Organization: Univ. València, Dep. Microbiologia
Lines: 17
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Hi netters!

First, my best wishes for the new year.

Then, one question.

We are working with mycorrhiza, and we have recently seen that a book was published
on 1981 about cultures of Lactarius: “Nutritional requirements of Lactarius 
species and cultural characters in relation to taxonomy”. A.J.P. Oort. 
North-Holland Pub. Co., 1981. ISBN 0444855335. I would like that you could 
give me data about or comment me this book, if you know something about it. 
Thank you in advance. Greetings.

  Sergi Ferrer                             Departament de Microbiologia
  Phone:  +34 (6) 3864390                       Facultat de Biologia 
  Fax:    +34 (6) 3864372                      Universitat de Valencia
  E-mail: Sergi.Ferrer@uv.es             E-46100 Burjassot-Valencia, SPAIN


From rhalling@nybg.org Mon Jan  9 21:32:15 EST 1995
Article: 1282 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!howland.reston.ans.net!spool.mu.edu!olivea!biosci!nybg.org!rhalling
From: rhalling@nybg.org
Newsgroups: bionet.mycology
Subject: Job announcement
Date: 9 Jan 1995 14:16:41 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 48
Sender: daemon@net.bio.net
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NNTP-Posting-Host: net.bio.net

Position Available
Herbarium Database Manager, New York Botanical Garden

The newly-created position of Herbarium Database Manager is currently available
at the New York Botanical Garden.  The Database Manager will be responsible for
the quality of the data entered into NYpc, the Garden's institution-wide
specimen database system.

The Herbarium Database Manager will train all new users of the database system.
Initially at least 10 herbarium staff members will catalog specimens in to the
database as a routine part of their job.  Training will include not only the
step-by-step operation of the database at the computer, but also interpretation
of specimen label data, including how to use pertinent botanical literature to
interpret that data. The Herbarium Database Manager will regularly review new
entries to the authority files associated with NYpc to be sure that the
information is correct and in the proper format.

The Herbarium Database Manager will be responsible for revising and maintaining
the user documentation for the software (both written and on-line), and will
work with the Computer Services Staff to help prepare specimen records to be
made available electronically to the scientific community via the INTERNET.
The Herbarium Database Manager will also help in the planning for,
implementation of, and conversion to the more elaborate database system (NYdb)
that will replace NYpc in the next five years.

The New York Botanical Garden is located in the northernmost part of New York
City, in the Bronx.  Our scientific program involves systematic and
ethnobotanical research on plants and fungi.  The herbarium of the New York
Botanical Garden is the largest in the western hemisphere, with approximately
5.6 million dried specimens.

The Herbarium Database Manager should have experience using a database, but
programming skills are not required.  Knowledge of plant nomenclature and
familiarity with botanical literature is essential.  The Database Manager
should have had experience working in an herbarium.  Because we will be
conducting databasing projects in all groups of plants and fungi, a familiarity
with a broad array of plant and fungal groups is desirable.  The Herbarium
Database Manager should have a B.S. or M.S. in Botany or Biology, or
equivalent experience.  Salary is commensurate with experience.  Excellent
fringe benefits, including four weeks vacation.  Send resume and letter of
interest to: Personnel Manager, New York Botanical Garden, 200 St. & Southern
Blvd., Bronx, NY 10458-5126.   AA/EOE/M/F/D/V

We would like to fill the position in February or March 1995.  If you have
questions about the job, contact: Barbara M. Thiers, Administrative Curator,
New York Botanical Garden, Bronx, NY 10458.  email: bthiers@nybg.org;
telephone: 718-817-6822; fax: 718-562-6780.



From jmicales@facstaff.wisc.edu Mon Jan  9 21:32:15 EST 1995
Article: 1283 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!hearst.acc.Virginia.EDU!caen!sol.ctr.columbia.edu!howland.reston.ans.net!news.moneng.mei.com!uwm.edu!news.doit.wisc.edu!F181-121.net.wisc.edu!jmicales
From: jmicales@facstaff.wisc.edu (Jessie Micales)
Newsgroups: bionet.mycology
Subject: Re: Abstract, Larson and Nuss, EMBO J.  13(1994), 5616-5623.
Date: Mon, 9 Jan 1995 21:20:39 GMT
Organization: U.S. Forest Service
Lines: 28
Distribution: world
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References: <9501091506.AA01970@mailgate.roche.com>
NNTP-Posting-Host: f181-121.net.wisc.edu

>Altered Transcriptional Response to Nutrient Availability in
>Hypovirus-Infected
>Chestnut Blight Fungus

>EMBO J.  13(1994), 5616-5623

>Thomas G. Larson and Donald L. Nuss

>Roche Institute of Molecular Biology
>Roche Research Center
>Nutley, NJ 07110, USA


>Abstract

>The gene lac-1, encoding the enzyme laccase, is one of several genes 
>of the chestnut blight fungus, Cryphonectria parasitica, that are suppressed 
>by virulence-attenuating mycoviruses of the genus Hypovirus.  

Oh, it does my heart good everytime I see the chestnut blight fungus called 
Cryphonectria parasitica.  Back in the days when it used to be Endothia, a 
certain member on my dissertation committee had kittens when my research 
conclusions supported the change to Cryphonectria.  She wanted me to 
rewrite my entire dissertation, but my major professor went to the Dean 
and had her taken off of my committee AFTER the defense.  He then 
checked into the hospital with stress-related heart pains!  What we go 
through for a Ph.D.!



From PAMKAZ@PPSERVER.TAMU.EDU Tue Jan 10 16:45:21 EST 1995
Article: 1284 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!howland.reston.ans.net!agate!biosci!PPSERVER.TAMU.EDU!PAMKAZ
From: PAMKAZ@PPSERVER.TAMU.EDU
Newsgroups: bionet.mycology
Subject: sign on
Date: 10 Jan 1995 08:32:26 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 2
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Message-ID: <212EEA330B4@ppserver.tamu.edu>
NNTP-Posting-Host: net.bio.net

Dear Sir/Madam:  How do I get signed on to receive the newsletter?  
Thanks, Pam at TAMU (PAMKAZ@ppserver.tamu.edu).


From greened@ava.BCC.ORST.EDU Tue Jan 10 16:45:22 EST 1995
Article: 1285 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!howland.reston.ans.net!spool.mu.edu!olivea!biosci!AVA.BCC.ORST.EDU!greened
From: greened@AVA.BCC.ORST.EDU (Diane_M Greene)
Newsgroups: bionet.mycology
Subject: Mouldy Thursday
Date: 10 Jan 1995 11:14:35 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
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Reply-To: Diane_M Greene <greened@ava.BCC.ORST.EDU>
NNTP-Posting-Host: net.bio.net

Mouldy Thursday (formerly OSU Mycological Society) has a meeting this 
Thursday, Jan 12,'95 in Cordley Hall, 4083(old herbarium library) Oregon 
State University at 12:00 NOON. 
This month Mike Castellano discusses the role of Fungi in the President's 
Plan. 
If you would like to give a presentation at a meeting let me know as soon 
as you can, we'll work it out.
pd

Diane Greene
greened@bcc.orst.edu




From stamets1@aol.com Tue Jan 10 22:15:18 EST 1995
Article: 1286 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!swiss.ans.net!newstf01.news.aol.com!not-for-mail
From: stamets1@aol.com (Stamets 1)
Newsgroups: bionet.mycology
Subject: Needing photographs of Psilocybe serbica & P. bohemica
Date: 10 Jan 1995 13:36:30 -0500
Organization: America Online, Inc. (1-800-827-6364)
Lines: 12
Sender: root@newsbf02.news.aol.com
Message-ID: <3euk3e$2fm@newsbf02.news.aol.com>
Reply-To: stamets1@aol.com (Stamets 1)

I am revising my first book, Psilocybe Mushrooms & their Allies - a
subject many of you may find disturbing or interesting depending
upon your point of view - and am trying to acquire high quality 
photographs of these of Psilocybe serbica, P. bohemica, and P.
subaeruginosa. . Any help would be appreciated.
They are to be used in a book going to press in April. I am paying
$ 100.00 per insertion for one time publishing rights with the originals
being returned to the sender. 

Thanks. 

paul stamets


From D.BRAYFORD@CABI.org Tue Jan 10 22:15:19 EST 1995
Article: 1287 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!hearst.acc.Virginia.EDU!caen!spool.mu.edu!agate!biosci!CABI.org!D.BRAYFORD
From: D.BRAYFORD@CABI.org ("David Brayford ", IMI)
Newsgroups: bionet.mycology
Subject: RE: CAB Mycology Abstracts
Date: 10 Jan 1995 07:22:04 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
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NNTP-Posting-Host: net.bio.net


Hi Lester
Yes, CAB databases are available as online services from several hosts,
depending on where you are.  They are also available on CD-ROM.
For details email:  CABI@CABI.ORG

(I can't send you details directly because I don't have your email address.)

Dave Brayford
International Mycological Institute
d.brayford@cabi.org
 -----------------------------------------------------------
From: BIOSCI-REQUEST
To: mycology
Subject: CAB Mycology Abstracts
Date: 04 January 1995 9:59

Hi there! Does anyone knows if the CAB Veterinary and Medical Mycology
abstracts, are offered on line by any Library or Service?, Do they charge?
Thanks
Lester Pasarell


From bncraig@bud.peinet.pe.ca Wed Jan 11 14:49:44 EST 1995
Article: 1288 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!howland.reston.ans.net!torn!news.unb.ca!peinet.pe.ca!peinet.pe.ca!not-for-mail
From: bncraig@bud.peinet.pe.ca (Brian N. Craig)
Newsgroups: bionet.mycology
Subject: Potato Blight Forecasting Models
Date: 10 Jan 1995 22:18:00 -0400
Organization: PEINet, Charlottetown, Prince Edward Island, Canada
Lines: 16
Message-ID: <3evf4o$g55@bud.peinet.pe.ca>
NNTP-Posting-Host: bud.peinet.pe.ca

I am looking for some good reference material and contacts (e-mail 
addresses) of researchers who have developed Late Blight Forecasting 
models for potatoes.

Research in the area of tomatoes as to blight forecasting models would 
also be valuable.

=======================================================================
|          Brian N. Craig            |          Agricultrue           |  
|    bncraig@bud.peinet.pe.ca        |   Pest Management Consultant   |      
|---------------------------------------------------------------------|
|         VOICE: (902) 368-5637      |         P.O. Box 1083          |
|           FAX: (902) 368-5661      |    Cornwall, P.E.I., Canada    |
|                                    |            C0A 1H0             |
=======================================================================



From jbrown@bbsrc.ac.uk Wed Jan 11 14:49:45 EST 1995
Article: 1289 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!howland.reston.ans.net!pipex!uknet!bcc.ac.uk!is.bbsrc.ac.uk!news
From: jbrown@bbsrc.ac.uk (james brown)
Newsgroups: bionet.mycology
Subject: Position announcement: plant pathologist
Date: 11 Jan 1995 18:35:24 GMT
Organization: John Innes Centre
Lines: 43
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Cereals Research Department
JOHN INNES CENTRE
Norwich, England

Plant Pathologist

The Cereals Research Department of the John Innes Centre is
internationally renowed for its research on the pathology, genetics,
molecular biology and physiology of wheat, barley and other cereal
crops.

Due to the imminent retirement of Dr Roy Johnson, we are seeking a
Plant Pathologist to lead the Centre's research programme on rusts of
cereals. The long-term aim of the cereal pathology programme is to
achieve durable genetic resistance to the important diseases of
cereals, including rusts. The successful candidate will initiate new
lines of research on the genetics and molecular biology of the
resistance of wheat and barley to yellow and brown rusts and of the
rust pathogens themselves, and will develop existing projects, to
maintain the Centre's leading position in this area. Applicants
should have an established record of outstanding research in applying
genetics to problems in plant pathology, and the potential to lead a
research programme of internationally recognised excellence.

The appointment will be in Pay Band 5 or 6 within the salary range
GBP13,286 to 29,700 per annum. A non-contributory superannuation
scheme is in operation. Further details of terms and conditions can
be provided on request. The position is funded by the Ministry of
Agriculture, Fisheries and Food, initially for a period of four years.
The JIC is an equal opportunities employer.

Applications must be made on a form, which can be obtained by writing
to The Personnel Officer, John Innes Centre, Norwich Research Park,
Colney, Norwich, NR4 7UH, telephone: 01603-452571 or fax: 01603-
456844, quoting reference CR/916. Informal inquiries from intending
applicants, about the scientific aspects of this post, may be
addressed to Dr James Brown at the John Innes Centre (phone number
above, fax: 01603-502241, e-mail: jbrown@bbsrc.ac.uk).

This position will be advertised in Nature on 19th January 1995, and
the closing date for the receipt of completed applications is 16th
Feb 1995.


From fbiol@linnaeus.bio.usyd.edu.au Wed Jan 11 21:50:16 EST 1995
Article: 1290 of bionet.mycology
Newsgroups: bionet.mycology,sci.bio.ecology
Path: bigblue.oit.unc.edu!concert!hearst.acc.Virginia.EDU!caen!sdd.hp.com!math.ohio-state.edu!uwm.edu!msunews!harbinger.cc.monash.edu.au!news.cs.su.oz.au!metro!news
From: Greg Pattinson <fbiol@linnaeus.bio.usyd.edu.au>
Subject: Re: Climate change and mycorrhizae
Message-ID: <D27pnn.41r@ucc.su.OZ.AU>
Sender: news@ucc.su.OZ.AU
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Organization: Information Services, Sydney University, Sydney, NSW, Australia
References:  <3enbod$n43@nntp1.u.washington.edu>
Date: Tue, 10 Jan 1995 23:09:22 GMT
Lines: 10
Xref: bigblue.oit.unc.edu bionet.mycology:1290 sci.bio.ecology:8185

A review paper Shafer S.R. and Schoeneberger M.M. (1994) Air pollution
and ecosystem health: The mycorrhizal connection covers effects of 
ozone, acid rain, carbon dioxide and general air pollutants on the
symbioses. It can be found in 
Pfleger F.L. and Linderman R.G.(ed)(1994) Mycorrhizae and Plant Health
APS Press St. Paul, Minnesota.

Regards Greg 




From msachs@ADMIN.OGI.EDU Mon Jan 16 13:02:13 EST 1995
Article: 1291 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!howland.reston.ans.net!spool.mu.edu!olivea!biosci!ADMIN.OGI.EDU!msachs
From: msachs@ADMIN.OGI.EDU (Matthew Sachs)
Newsgroups: bionet.mycology
Subject: Re: Neurospora codon usage
Date: 11 Jan 1995 13:26:36 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
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>Does anyone know of a published codon usage table for
>Neurospora?  Thanks for your help
>
>Walt Lilly
>c749scb@semovm.semo.edu

The most recent:

Edelmann, S. E., and C. Staben. 1994. A statistical analysis of sequence
features within genes from Neurospora crassa. Exp. Mycol. 18:70-81.

Use of codon-preference analysis to discriminate exons and introns in N.
crassa genomic DNA sequences:

Sachs, M. S., H. Bertrand, R. L. Metzenberg, and U. L. RajBhandary. 1989.
Cytochrome oxidase subunit V gene of Neurospora crassa: DNA sequences,
chromosomal mapping, and evidence that the cya-4 locus specifies the
structural gene for subunit V. Mol. Cell. Biol. 9:566-577.




-----------------------------------------------------------
Matthew Sachs
Department of Chemistry, Biochemistry and Molecular Biology
Oregon Graduate Institute of Science and Technology
20000 NW Walker Road
P.O. Box 91000
Portland, OR  97291-1000
503 690-1487 Phone
503 690-1464 Fax
msachs@admin.ogi.edu




From C749SCB@SEMOVM.SEMO.EDU Mon Jan 16 13:02:14 EST 1995
Article: 1292 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!howland.reston.ans.net!spool.mu.edu!olivea!biosci!SEMOVM.SEMO.EDU!C749SCB
From: C749SCB@SEMOVM.SEMO.EDU (Walt Lilly)
Newsgroups: bionet.mycology
Subject: Neurospora codon usage
Date: 11 Jan 1995 11:42:21 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
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Does anyone know of a published codon usage table for
Neurospora?  Thanks for your help

Walt Lilly
c749scb@semovm.semo.edu



From brambl@molbio.cbs.umn.edu Mon Jan 16 13:02:15 EST 1995
Article: 1293 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!howland.reston.ans.net!spool.mu.edu!olivea!biosci!GRAZ.CBS.UMN.EDU!brambl
From: brambl@GRAZ.CBS.UMN.EDU (Robert Brambl)
Newsgroups: bionet.mycology
Subject: Re:  Neurospora codon usage
Date: 11 Jan 1995 13:49:40 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
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The following is a slightly dated table for Neurospora crassa, based  
upon 38 genes found in GenBank 63, as produced by J. Michael Cherry  
(cherry@frodo.mgh.harvard.edu) with the GCG program CodonFrequency.

Duplicates, pseudogenes, mutant and synthetic genes were not  
included. Coding regions were specified using the Feature Table of  
each entry, then checked for accuracy. If more than one stop codon  
was found the sequence was not included.

AmAcid  Codon     Number    /1000     Fraction   ..
 

Gly     GGG       85.00      6.31      0.09
Gly     GGA      133.00      9.88      0.14
Gly     GGT      312.00     23.17      0.34
Gly     GGC      394.00     29.26      0.43
 

Glu     GAG      494.00     36.69      0.76
Glu     GAA      159.00     11.81      0.24
Asp     GAT      218.00     16.19      0.38
Asp     GAC      358.00     26.59      0.62
 

Val     GTG      159.00     11.81      0.20
Val     GTA       72.00      5.35      0.09
Val     GTT      179.00     13.29      0.23
Val     GTC      380.00     28.22      0.48
 

Ala     GCG      177.00     13.15      0.16
Ala     GCA      136.00     10.10      0.12
Ala     GCT      271.00     20.13      0.25
Ala     GCC      513.00     38.10      0.47
 

Arg     AGG      167.00     12.40      0.15
Arg     AGA      130.00      9.66      0.12
Ser     AGT       85.00      6.31      0.07
Ser     AGC      195.00     14.48      0.17
 

Lys     AAG      478.00     35.50      0.90
Lys     AAA       52.00      3.86      0.10
Asn     AAT       73.00      5.42      0.18
Asn     AAC      328.00     24.36      0.82
 

Met     ATG      254.00     18.87      1.00
Ile     ATA       52.00      3.86      0.10
Ile     ATT      162.00     12.03      0.30
Ile     ATC      319.00     23.69      0.60
 

Thr     ACG      153.00     11.36      0.19
Thr     ACA      133.00      9.88      0.17
Thr     ACT      144.00     10.70      0.18
Thr     ACC      366.00     27.18      0.46
 

Trp     TGG      219.00     16.27      1.00
End     TGA      132.00      9.80      0.74
Cys     TGT      102.00      7.58      0.33
Cys     TGC      209.00     15.52      0.67
 

End     TAG       17.00      1.26      0.10
End     TAA       29.00      2.15      0.16
Tyr     TAT      106.00      7.87      0.29
Tyr     TAC      263.00     19.53      0.71
 

Leu     TTG      168.00     12.48      0.14
Leu     TTA       65.00      4.83      0.05
Phe     TTT      129.00      9.58      0.28
Phe     TTC      330.00     24.51      0.72
 

Ser     TCG      227.00     16.86      0.20
Ser     TCA      140.00     10.40      0.12
Ser     TCT      211.00     15.67      0.18
Ser     TCC      300.00     22.28      0.26
 

Arg     CGG      160.00     11.88      0.15
Arg     CGA      127.00      9.43      0.12
Arg     CGT      213.00     15.82      0.19
Arg     CGC      306.00     22.73      0.28
 

Gln     CAG      278.00     20.65      0.59
Gln     CAA      192.00     14.26      0.41
His     CAT      161.00     11.96      0.39
His     CAC      252.00     18.72      0.61
 

Leu     CTG      245.00     18.20      0.20
Leu     CTA       89.00      6.61      0.07
Leu     CTT      216.00     16.04      0.18
Leu     CTC      451.00     33.50      0.37
 

Pro     CCG      197.00     14.63      0.20
Pro     CCA      190.00     14.11      0.19
Pro     CCT      242.00     17.97      0.24
Pro     CCC      367.00     27.26      0.37


From fgsc@kuhub.cc.ukans.edu Mon Jan 16 13:02:16 EST 1995
Article: 1294 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!rutgers!gatech!howland.reston.ans.net!news.starnet.net!wupost!kuhub.cc.ukans.edu!fgsc
From: fgsc@kuhub.cc.ukans.edu
Newsgroups: bionet.mycology
Subject: Position announcement
Message-ID: <1995Jan11.100522.82362@kuhub.cc.ukans.edu>
Date: 11 Jan 95 10:05:22 CST
Organization: University of Kansas Academic Computing Services
Lines: 71

Position Available: 
Curator for the Fungal Genetics Stock Center 

Craig Wilson has decided to resign as Curator of the Fungal Genetics
Stock Center in order to pursue his growing interests in
Library/Information Science.  For many of you who have
come to depend upon Craig for his quick and reliable help this information
will come as somewhat of a blow  (as it did to me).  Craig has done an
outstanding job and will be sorely missed by those of us who worked with
him.  However, this situation presents an opportunity for someone with the
right skills and personality traits who might be looking for such a position. 
As things now stand the position does not require a Ph. D., but it could be
a good position for someone with a Ph. D. who does not wish to establish
his/her own lab and pursue the chase for research grants.  If you know of
someone with academic interests in Fungal Genetics who might be
interested in this position please have them contact me.  Below I have
asked Craig to list some of his regular activities  to give you a better feel
for the skills required for the job.

John A. Kinsey
Department of Microbiology
University of Kansas Medical Center
Kansas City, KS 66160
Ph. 913-588-7088
Fax 913-588-7295
E-mail jkinsey@kuhub.cc.ukans.edu

Ongoing activities of the stock center (at any time of year):

Respond to requests for strains
Preserve new deposits of fungi and DNA
Test characteristics of new deposits
Update records for printed/online catalogues
Solicit deposits of newly developed and/or important strains/genes

Seasonal activities:

Compile, compose, print, and distribute the Fungal Genetics Newsletter
Compile, compose, and print meeting programs/proceedings from Fungal
Genetics
     Conferences
Print periodic updates of FGSC catalogue
Collect and record reprints from annual bibliographies


This rather terse listing of core functions of the stock center neglects things
that could be done to improve and expand the collection and information
related to it.  I have spent a lot of time in the past year making stock lists,
meeting abstracts, newsletter articles, the Neurospora Compendium, etc.
available to readers via the World Wide Web.  There is surely additional
relevant data that could be presented in this forum.

Over the years, I have had the chance to find map locations for a few
unmapped mutations represented in FGSC, but more remain.  I have done
virtually nothing about developing cloned materials in the collection.  As
the effort to develop truly ordered libraries of fungal sequences proceeds,
the stock center should be involved in developing, preserving and
distributing these and related tools.  Someone with more molecular skill
than I have could undertake useful development projects I can't even
imagine.

It has been my privilege to be responsible for this vital collection and I
can't overstate what a terrific opportunity it provides for someone who can
bring energy and enthusiasm to improving, expanding, and developing a
facility that is of such benefit to researchers around the world.  I, too,
would be happy to speak to persons with specific questions about what
it is like working at FGSC.

Craig Wilson
ph. 913-588-7044
e-mail fgsc@kuhub.cc.ukans.edu


From bernii@aol.com Mon Jan 16 13:02:17 EST 1995
Article: 1295 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!rutgers!gatech!swiss.ans.net!newstf01.news.aol.com!not-for-mail
From: bernii@aol.com (Bernii)
Newsgroups: bionet.mycology
Subject: slime molds???
Date: 11 Jan 1995 13:01:01 -0500
Organization: America Online, Inc. (1-800-827-6364)
Lines: 8
Sender: root@newsbf02.news.aol.com
Message-ID: <3f16ct$cp8@newsbf02.news.aol.com>
Reply-To: bernii@aol.com (Bernii)

For an article for NICKELODEON MAGAZINE (for kids 8-12 ish) I am 
researching slime molds, particularly Myxomycetes (esp. Physarum). I have
some basic stuff, but looking for more.

Very interested in finding someone who has kept Plasmodia in the lab or at
home, and considered it a "pet."

Any help is appreciated. Thanks!


From jonnyt@kjemi.tih.no Mon Jan 16 13:02:19 EST 1995
Article: 1296 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!howland.reston.ans.net!news.sprintlink.net!pipex!warwick!news.dcs.warwick.ac.uk!hgmp.mrc.ac.uk!daresbury!trane.uninett.no!astfgl.edb.tih.no!pc036.kjemi.tih.no!jonnyt
From: jonnyt@kjemi.tih.no (jonny tverdal)
Newsgroups: bionet.mycology
Subject: lichen, growth of cladonia i culture, media
Date: Thu, 12 Jan 1995 16:18:16
Organization: Institutt for Kjemi, HIST
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NNTP-Posting-Host: pc036.kjemi.tih.no
X-Newsreader: Trumpet for Windows [Version 1.0 Rev A]

Hello!!

We are two students at Soer-Troendelag College in Trondheim Norway.
In connection with a practical work we need information 
on growing lichen, specially Cladonia species in culture-media 
composition, growth conditions, how to avoid contamination.
We have tried to do litterature research on the subject, but it is 
really hard to find relevant litterature.

If You have any information, please send it by E.mail to:
Jonnyt@kjemi.tih.no     or
Arildh@kjemi.tih.no 


Thanks!!! 
Hilsen Jonny.


From bq673@FreeNet.Carleton.CA Mon Jan 16 13:02:20 EST 1995
Article: 1297 of bionet.mycology
Newsgroups: bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!howland.reston.ans.net!torn!nott!cunews!freenet.carleton.ca!FreeNet.Carleton.CA!bq673
From: bq673@FreeNet.Carleton.CA (Stefan Voroney)
Subject: Biologicals for sale?
Message-ID: <D2Cvqq.G4w@freenet.carleton.ca>
Sender: bq673@freenet2.carleton.ca (Stefan Voroney)
Organization: The National Capital FreeNet, Ottawa, Ontario, Canada
Date: Fri, 13 Jan 1995 18:08:50 GMT
Lines: 8


I am new to the field of mycology and I wish to know if there are
any companies in Canada (or the US) which sell cultures and/or
spore prints of various basidiomycetes around that a layperson
can order from. It gets frustrating going on field hunts all the 
time.... Catalogues of suppliers to professionals would be OK too
if available. I'm counting on user input so please help!



From niemirab@student.msu.edu Mon Jan 16 13:02:21 EST 1995
Article: 1298 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!psuvax1!uwm.edu!msunews!safir.bpp.msu.edu
From: niemirab@student.msu.edu  (Brendan A. Niemira)
Newsgroups: bionet.mycology,sci.bio.ecology
Subject: Re: Climate change and mycorrhizae
Date: Fri, 13 Jan 1995  12:43 EST
Organization: Michigan State University
Lines: 17
Distribution: world
Message-ID: <19950113124324.niemirab@safir.bpp.msu.edu>
References: <3enbod$n43@nntp1.u.washington.edu>
NNTP-Posting-Host: safir.bpp.msu.edu
X-Newsreader: FTPNuz (DOS) v1.0
Xref: bigblue.oit.unc.edu bionet.mycology:1298 sci.bio.ecology:8219

In Article <3enbod$n43@nntp1.u.washington.edu> "boe666@u.washington.edu (Peter Werner)" says:
> I'm going to be writing a research paper on the effects of several types 
> of environmental disruption on mycorrhizae and how this in turn effects 
> forest health.  So far, I've been able to find quite a bit of literature 
> on the effects of acid precipitation on mycorrhizae, as well as some 
> literature on the effects of disturbances such as logging.
> 
> What I haven't been able to find is anything about the effects of climate 
> change on mycorrhizal fungi.  Changes in temperature and, especially, 
> precipitation patterns would seem to have a strong effect on these fungi, 
> significantly altering the distribution of these species, at the very 
> least.  If anyone knows of any literature that has been written about
> this subject, I'd greatly appreciate the reference.
> 
> Thanks in advance,
> PGW
> 


From wharrison@aol.com Mon Jan 16 13:02:23 EST 1995
Article: 1299 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!newsfeed.pitt.edu!uunet!newstf01.news.aol.com!not-for-mail
From: wharrison@aol.com (WHARRISON)
Newsgroups: bionet.mycology
Subject: Spores Afield avail free via Internet
Date: 13 Jan 1995 19:18:48 -0500
Organization: America Online, Inc. (1-800-827-6364)
Lines: 20
Sender: root@newsbf02.news.aol.com
Message-ID: <3f7598$95j@newsbf02.news.aol.com>
Reply-To: wharrison@aol.com (WHARRISON)

Spores Afield, the monthly newsletter of the Colorado Mycological Society,
is available via Internet email.  It is the first Mycological Society
newsletter to go out on the Internet.  The first issue of the new year
will come out in late February.

To send articles, comments, subscription requests, etc., to Spores Afield,
address them to sporescms@aol.com (or just sporescms on America Online).

ECONET also carries back copies of Spores Afield, in addition to a
compilation of interesting articles from the past three years in files
called "The Best of Spores".  ECONET is available on Internet.  Point a
Gopher at  gopher.igc.apc.org (uses default port 70)
 

Wayne Harrison   wharrison@aol.com             
Pine, Colorado      ____________         
elev. 8,500 feet  /  (mushroom)  \
"pray for rain"   \______________/              
                       |    |
                      /      \


From greened@ava.BCC.ORST.EDU Mon Jan 16 13:02:24 EST 1995
Article: 1300 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!news-server.ncren.net!news.duke.edu!godot.cc.duq.edu!hudson.lm.com!news.pop.psu.edu!news.cac.psu.edu!howland.reston.ans.net!spool.mu.edu!olivea!biosci!AVA.BCC.ORST.EDU!greened
From: greened@AVA.BCC.ORST.EDU (Diane_M Greene)
Newsgroups: bionet.mycology
Subject: Mouldy Thursday
Date: 12 Jan 1995 19:42:15 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
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Sender: daemon@net.bio.net
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Reply-To: Diane_M Greene <greened@ava.BCC.ORST.EDU>
NNTP-Posting-Host: net.bio.net

3 things:

Our scheduling for the next 2 months:

Feb. 2, 1995	Dave Pilz	Forest Service Research on 
				Edible Fungi of the Northwest that 
				are Collected as Special Forest 
				Products

Mar. 2, 1995	Joey Spatafora	to be announced

Meetings are at Cordley 4083 (old herbarium library) at Oregon State 
		University, Corvallis, Oregon
Noon, first Thursday of each month (that is to say, the Mouldy Thursday)
 

Next, I have tentatively nominated a few persons to serve on 
committees(so I can do my homework):

Internet Communications: Tina Dreisbach, and anyone else who knows
			anything!!
Planning: Randy Molina, Melodie Putnam

Databasing: Sherry Pittam, Diane Greene

Newsletter: Jim Eblin, Dave Pilz

I chose these only by recommendation and observation. The committees need 
more folks to do more. Please volunteer for one of these committees if 
you can. It need only take as much time as you can afford.

To avoid taking valuable talk time, I've scheduled a committees meeting 
for Thursday Jan 26, 1995 at Cordley 4083 at noon. Please come if you 
have any input at all, or email me.

Lastly, I've gotten out of state interest in Mouldy Thursday. I would like 
to welcome Scott Redhead and Don Reynolds. The other out of staters are 
by default.

pd



From npk3325@VENUS.TAMU.EDU Mon Jan 16 13:02:25 EST 1995
Article: 1301 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!news-server.ncren.net!taco.cc.ncsu.edu!biosci!VENUS.TAMU.EDU!npk3325
From: npk3325@VENUS.TAMU.EDU (Nancy Keller)
Newsgroups: bionet.mycology
Subject: M.fructicola mummies
Date: 12 Jan 1995 09:55:12 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 23
Sender: daemon@net.bio.net
Distribution: world
Message-ID: <199501121755.JAA19332@net.bio.net>

I need help again.  
How do you induce apothecia formation with:
(a) a M. fructicola mummy
        History: I let a nectarine mummify; it is currently outside under
my banana plant (which is an indirect way to say that I don't know if it
gets cold enough in Texas to induce apothecial formation).  I want to show
apothecia for the Ascomycete lab I'm teaching this spring.  Should I put
the mummy in the refrigerator for a period of time and then bring it up to
room temperature?  How many days does it take for a apothecia to form?

(b) a Sclerotinia sclerotium?

If anyone has hints on the above, I'd appreciate any ideas.
Thanks,

Nancy P. Keller
Dept. Plant Pathology and Microbiology
Texas A&M University
College Station, TX 77843-2132
409-845-0963 P
409-845-8463 F
npk3325@venus.tamu.edu



From ksanders@ccinet.ab.ca Mon Jan 16 13:02:26 EST 1995
Article: 1302 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!newsxfer.itd.umich.edu!agate!library.ucla.edu!csulb.edu!nic-nac.CSU.net!charnel.ecst.csuchico.edu!waldorf.csc.calpoly.edu!decwrl!tribune.usask.ca!quartz.ucs.ualberta.ca!finzi.ccinet.ab.ca!news@ccinet.ab.ca
From: ksanders@ccinet.ab.ca (Ken Sanderson)
Newsgroups: bionet.mycology
Subject: Re: Needing photographs of Psilocybe serbica & P. bohemica
Date: 14 Jan 1995 07:06:01 GMT
Organization: The Job Track
Lines: 9
Message-ID: <3f7t4q$lsh@finzi.ccinet.ab.ca>
References: <3euk3e$2fm@newsbf02.news.aol.com>
NNTP-Posting-Host: fortmacr2.ccinet.ab.ca
X-Newsreader: WinVN 0.90.3

In article <3euk3e$2fm@newsbf02.news.aol.com>, stamets1@aol.com (Stamets 1) says:
>
>I am revising my first book, Psilocybe Mushrooms & their Allies - a


Will this book be published through ten speed press?  And when do you 
expect it to hit the stands?

Thanks Ken


From esquerre@cict.fr Mon Jan 16 13:02:27 EST 1995
Article: 1303 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!news-server.ncren.net!taco.cc.ncsu.edu!biosci!bcm!cs.utexas.edu!howland.reston.ans.net!news.sprintlink.net!pipex!uknet!daresbury!not-for-mail
From: "Esquerre" <esquerre@cict.fr>
Newsgroups: bionet.mycology
Subject: Post doctoral research position
Date: 12 Jan 1995 15:21:19 -0000
Lines: 16
Sender: lpddist@mserv1.dl.ac.uk
Distribution: bionet
Message-ID: <3f3hdf$bfk@mserv1.dl.ac.uk>
Reply-To: <esquerre@cict.fr>
X-Minuet-Version: Minuet1.0_Beta_15
Original-To: mycology@dl.ac.uk

URGENT!
Applications are invited for a research project on the regulation of 
endopolygalacturonase gene expression in a phytopathogenic fungus.
Applicants should have a strong background in current molecular biology 
techniques ; experience in transformation of fungi would be an advantage.
The appointment is for one year, starting immediately ; salary will be 
about 13000 FF per month. Please contact us rapidly by e-mail, and send a 
cv including list of publications and names, addresses and telephone 
nunbers of 2 referees.
Marie-Therese Esquerre-Tugaye, Professor
Laboratoire Interactions Plantes-Microorganismes      Tel.(33) 61 55 67 59
Centre de Biologie et Physiologie V g tales           Fax (33) 61 55 83 78
URA CNRS 1457
Universit  Paul Sabatier
118, route de Narbonne
31062 Toulouse cedex, France


From plantmed@aol.com Mon Jan 16 13:02:29 EST 1995
Article: 1304 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!swiss.ans.net!newstf01.news.aol.com!not-for-mail
From: plantmed@aol.com (PlantMed)
Newsgroups: bionet.mycology
Subject: fungus fair
Date: 14 Jan 1995 13:32:00 -0500
Organization: America Online, Inc. (1-800-827-6364)
Lines: 2
Sender: root@newsbf02.news.aol.com
Message-ID: <3f95b0$gu9@newsbf02.news.aol.com>
Reply-To: plantmed@aol.com (PlantMed)

The annual Fungus Fair is being held in Santa Cruz this weekend (1/14-15).
 If you live in the area, drop on by.  It's in Harvey West Park.


From MREDARD@FUNDCH.FUNDCH.CL Mon Jan 16 13:02:30 EST 1995
Article: 1305 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!news-server.ncren.net!news.duke.edu!godot.cc.duq.edu!hudson.lm.com!news.pop.psu.edu!news.cac.psu.edu!howland.reston.ans.net!spool.mu.edu!olivea!biosci!FUNDCH.FUNDCH.CL!MREDARD
From: MREDARD@FUNDCH.FUNDCH.CL (Marcela Redard A)
Newsgroups: bionet.mycology
Subject: Boletus eludis
Date: 12 Jan 1995 04:16:46 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 13
Sender: daemon@net.bio.net
Distribution: world
Message-ID: <9501121217.AA26200@fundch.cl>

Hi!
I would really appreciate if somebody could help me. I am looking
for some commercial information specifically current trading
prices and traded volumes about Boletus edulis.
I have been searching with no positive result so if you know some
references about this, please send it to me.
Thanks in advance,
Regards,

Paula.

mredard@fundch.fundch.cl



From jbrown01@BIGCAT.MISSOURI.EDU Mon Jan 16 13:02:30 EST 1995
Article: 1306 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!news-server.ncren.net!news.duke.edu!solaris.cc.vt.edu!news.alpha.net!uwm.edu!spool.mu.edu!olivea!biosci!BIGCAT.MISSOURI.EDU!jbrown01
From: jbrown01@BIGCAT.MISSOURI.EDU ("John M. Brown")
Newsgroups: bionet.mycology
Subject: Pleurotus citrinopileatus
Date: 12 Jan 1995 19:52:59 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 17
Sender: daemon@net.bio.net
Distribution: world
Message-ID: <Pine.3.89.9501122119.A25513-0100000@bigcat>
NNTP-Posting-Host: net.bio.net


Hi, I am just becomming interested in mushrooms and would like 
information on growing the Golden Oyster (Pleurotus citrinopileatus), and 
a source for innoculum.

Secondly, does anyone have a source for used glass petri dishes?

I have a used rather large autoclave without a steam generator.  The 
autoclave is about the size of a 50 gallon barrel and appears in good 
shape.  I have never used the unit.  

Thanks, and hope to be communicating more often.  

Sincerely,
John M. Brown
Columbia, MO
314-442-6273


From kasplash@crl.com Mon Jan 16 13:02:32 EST 1995
Article: 1307 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!news-server.ncren.net!news.duke.edu!agate!howland.reston.ans.net!nntp.crl.com!crl.crl.com!not-for-mail
From: kasplash@crl.com (Bob Galvan)
Newsgroups: bionet.mycology
Subject: Mushroom ID in Marin
Date: 14 Jan 1995 12:06:17 -0800
Organization: CRL Dialup Internet Access	(415) 705-6060  [login: guest]
Lines: 16
Distribution: usa
Message-ID: <3f9arp$t0e@crl.crl.com>
NNTP-Posting-Host: crl.com

Greetings,   I am a budding mushroom hunter in Marin county, California.
I am hoping to find some people who live nearby and can help me get 
started with places to go, species to look for, and identification.  I do 
have some background in botany and know the county fairly well.  I am 
aware of Amanitas, Boletes, Chantrelles, Morelles, and have one good book,
Demystifying Mushrooms.

It has been raining nonstop for weeks here in Marin and the shrooms are 
poppin up everywhere!

Bob Galvan
kasplash@crl.com
415-924-5384





From fzrichrd@bullwinkle.ucdavis.edu Mon Jan 16 13:02:33 EST 1995
Article: 1308 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!news-server.ncren.net!taco.cc.ncsu.edu!gatech!europa.eng.gtefsd.com!library.ucla.edu!news.ucdavis.edu!bullwinkle!fzrichrd
From: fzrichrd@bullwinkle.ucdavis.edu (Rebecca Richards)
Newsgroups: bionet.mycology
Subject: Japanese cultural use of matsutake
Date: 13 Jan 1995 21:10:51 GMT
Organization: University of California, Davis
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X-Newsreader: TIN [version 1.2 PL2]

I would appreciate learning more about the historical and contemporary
use and significance of matsutake (Tricholoma magnivelare = North
American species) in Japan.  Thanks in advance.

Becky Richards, UC Davis


From stamets1@aol.com Mon Jan 16 13:02:33 EST 1995
Article: 1309 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!news-server.ncren.net!taco.cc.ncsu.edu!gatech!howland.reston.ans.net!pipex!uunet!newstf01.news.aol.com!not-for-mail
From: stamets1@aol.com (Stamets 1)
Newsgroups: bionet.mycology
Subject: Re: Needing photographs of Psilocybe serbica & P. bohemica
Date: 14 Jan 1995 15:51:50 -0500
Organization: America Online, Inc. (1-800-827-6364)
Lines: 67
Sender: root@newsbf02.news.aol.com
Message-ID: <3f9dh6$hup@newsbf02.news.aol.com>
References: <3f7t4q$lsh@finzi.ccinet.ab.ca>
Reply-To: stamets1@aol.com (Stamets 1)



Ken et alia....                                                           
              1/14/95

The book is currently 212 pages long (ms.) and, although Ten Speed wants
it, I do not yet have a contract. This book is alot of fun to write and is
a 
subject with which I have had long experience. Since several young people
 have died mistaking a Galerina for a Psilocybe, the book will
serve to protect public health. (Did you know there were mycologists
 in the 1970's who stated it was better for people to die from mistaken
identification than to provide information on the accurate identification
of these species?!?) Many physcians have asked me for copies of my
original version of Psilocybe Mushrooms & their Allies and at their
urging -as well as from others- I am reworking the subject.. Since I now
have control over the production of my books, I feel I can do a much
better job than what appeared in 1978.  



Despite this book's subject, our business will 
continue with its long standing policy of 

-not offering strains, spores or cultures
-not offerring advice on the cultivation of these mushrooms
-not discussingthe subject of Psilocybe except through book sales


The work I have done was covered by a DEA license, 
and has led to the publication of two, soon three, new species
 of Psilocybe. I am particularly fascinated by the truly natural
 habitats for Psilocybes. The proliferation of  woodchips for 
landscaping has provided a convenient habitat in urban and
suburban areas, but the active Psilocybes are exceedingly rare
 in natural forests. We have found good evidence of small mammal burrows
harboring these species in deciduous woods.... Many species are
 undoubtedly imported with ornamental plants and/or hitchhiked by
 other means as international travellers. Finding truly indigenous 
species is quite a task. Any suggestions, leads or references would be
appreciated. For instance, who has ever  re-collected Psilocybe
 angusticeps and Psilocybe washingtonensis (Smith species) from 
Washington? These two, apparently non-active species
seem like taxonomic phantoms - described once and 
have seemingly disappeared from the ecosystem (or from
the collection baskets of mycologists!)  Has
 anyone recollected/photographed P. tampanensis from Florida?
 What about Murrill's report of P. caerulescens
>from  Alabama? What of Peck's citing of a mushroom resembling
 P. semilanceata near Albany, New York? What were the 
mushrooms (Panaeoli ?)  responsible for the Maine intoxications/parties
 as reported by Lincoff and Mitchel earlier in this century? What is
the strongly bluing mushroom resembling P. pelliculosa from Telluride, Co?
(One specimen collected from a trail frequented by several hundred
mycophiles over 13 years.) Chang & Mills report synonomy with
 Australian taxa which inhabit
wood chips and manure - two ends of a spectrum of habitats. 
 Although common in disturbed habitats, 
these natural citings are exceedingly rare. Who out there has
found an active Psilocybe in a woodland habitat truly
considered untouched by humans? 
Any comments would be appreciated, you can e-mail me
directly, if you wish.

Thanks. 

paul stamets


From staben@ukcc.uky.edu Mon Jan 16 13:02:34 EST 1995
Article: 1310 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!newsxfer.itd.umich.edu!jobone!ukma!service1.uky.edu!news
From: Chuck Staben <staben@ukcc.uky.edu>
Newsgroups: bionet.mycology
Subject: apoptosis
Date: 11 Jan 1995 00:02:36 GMT
Organization: A poorly-installed InterNetNews site
Lines: 1
Message-ID: <3ev76s$c1m@service1.uky.edu>
NNTP-Posting-Host: 128.163.21.206

Does apoptosis occur in fungi?  Aspergillus nimA can cause chromatin condensation, but it is not clear to me whether this is apoptosis as defined in a mammalian system.  Any feedback?


From mayberry@gulf.net Mon Jan 16 13:02:35 EST 1995
Article: 1311 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!europa.eng.gtefsd.com!usenet.eel.ufl.edu!cobia.gulf.net!gulf.net!mayberry
From: mayberry@gulf.net (Don Mayberry)
Newsgroups: bionet.mycology
Subject: genus Hanseniaspora
Date: Mon, 16 Jan 1995 01:32:54
Organization: The Gulf Coast Internet Company
Lines: 6
Message-ID: <mayberry.1.00018C7C@gulf.net>
NNTP-Posting-Host: slip9.gulf.net
Summary: I would appreciate any information on the genus Hanseniaspora
Keywords: yeast: Hanseniaspora
X-Newsreader: Trumpet for Windows [Version 1.0 Rev A]

I work in a clinical mycology laboratory and we
recently isolated a yeast from the finger of a child 
which identified as in the genus Hanseniaspora. I would
appreciate any information about the clinical significance
of this yeast. Thank you
 Nancy Norris


From arnold@bscr.uga.edu Mon Jan 16 13:02:36 EST 1995
Article: 1312 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!howland.reston.ans.net!agate!biosci!GENOME.GENETICS.UGA.EDU!arnold
From: arnold@GENOME.GENETICS.UGA.EDU
Newsgroups: bionet.mycology
Subject: (none)
Date: 16 Jan 1995 05:31:43 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 40
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Message-ID: <0098A8A4.0BE503D1.39@genome.genetics.uga.edu>
Reply-To: arnold@bscr.uga.edu
NNTP-Posting-Host: net.bio.net


01/14/94

We need the map position of the following genes to link
the genetic and physical maps of all eight chromosomes
of Aspergillus nidulans.

                           Flanking Markers
                      -------------------------
Marker		      Right Marker   Left Marker  Chromosome
------------------------------------------------------------
phytochrome			        		?
pelA					        	?
penN (part of npeA)				       VI
bimC						       VI
bimE					               VI
acoD(fluG)					       III
klpA						       I
nimA					               III
nimE						       II
nudC						       V
spoC1						       III
tubB						       IV
tubC						       I
actin					               I
pCAN26						       VII
pCAN12						       VIII
Histone H3					       VIII
Histone H4				               VIII

If you have the map position and/or complementation data (complementing clone
names from the FGSC A. nidulans library) of other genes not listed
in the FGSC catalog, we would also appreciate this information as well. 


Yours,


Jonathan Arnold
arnold@bscr.uga.edu


From ddeshaz@cello.gina.calstate.edu Mon Jan 16 13:02:37 EST 1995
Article: 1313 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!bloom-beacon.mit.edu!usc!nic-nac.CSU.net!cello.gina.calstate.edu!swrl23.slip.csu.net!ddeshaz
From: Darvin A. DeShazer <ddeshaz@cello.gina.calstate.edu>
Newsgroups: bionet.mycology
Subject: Fungus Fair
Date: 16 Jan 1995 06:19:08 GMT
Organization: Science Chairperson
Lines: 16
Distribution: world
Message-ID: <3fd34s$hjt@cello.gina.calstate.edu>
NNTP-Posting-Host: swrl23.slip.csu.net
X-UserAgent: 1.1
X-XXMessage-ID: <AB3F50BF1C010117@swrl23.slip.csu.net>
X-XXDate: Sun, 15 Jan 95 01:22:55 GMT

The Sonoma County Mycological Association (SOMA) would like to invite the
world to our annual Mushroom Fair.  It will be held on Sunday, January
22, 1995 at the Coddingtown Mall in Santa Rosa, California, USA.  All
interested mushroomers are welcome to attend for free!!
_______________________________________________________________________
_______
                             _______________
Darvin DeShazer             [               ]
Science Dept. Chair, SVHS    ===============             May the smell of
Science Advisor, SOMA             |   |                   Matsutake fill 
                                  |___|                    your    - -
                                 /.....\                            ^
      ______                      |   |       ______              \___/
     [      ]                      \   \     [      ]
      ======                        \   \     ======
       |  |                          ----      |  |


From jonnyt@kjemi.tih.no Tue Jan 17 10:11:46 EST 1995
Article: 1314 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!swrinde!pipex!sunic!ugle.unit.no!trane.uninett.no!astfgl.edb.tih.no!pc041.kjemi.tih.no!jonnyt
From: jonnyt@kjemi.tih.no (jonny tverdal)
Newsgroups: bionet.mycology
Subject: Lichen, growth of cladonia in culture, media?
Date: Tue, 17 Jan 1995 11:37:40
Organization: Institutt for Kjemi, HIST
Lines: 19
Message-ID: <jonnyt.10.000BA119@kjemi.tih.no>
NNTP-Posting-Host: pc041.kjemi.tih.no
X-Newsreader: Trumpet for Windows [Version 1.0 Rev A]

Hello!!!

We are two students at Soer-Troendelag College in Trondheim Norway.
In connection with a practical work we shall do, we need information 
on growing lichen, specially Cladonia species in culture-media 
composition, growth conditions, how to avoid contamination.
Our biggest problem right now is that we must have a medium to start with 
growth on. We have tried to do litterature research on the subject, but it is 
really hard to find relevant litterature.

If You have any information, please send it by E.mail to:
Jonnyt@kjemi.tih.no     or
Arildh@kjemi.tih.no 


Thanks!!! 

Jonny &Arild.
Hilsen Jonny.


From jonnyt@kjemi.tih.no Tue Jan 17 10:11:47 EST 1995
Article: 1315 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!swrinde!pipex!sunic!ugle.unit.no!trane.uninett.no!astfgl.edb.tih.no!pc038.kjemi.tih.no!jonnyt
From: jonnyt@kjemi.tih.no (jonny tverdal)
Newsgroups: bionet.mycology
Subject: Cladonia cristatella, medium to grow?
Date: Tue, 17 Jan 1995 14:16:00
Organization: Institutt for Kjemi, HIST
Lines: 13
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NNTP-Posting-Host: pc038.kjemi.tih.no
X-Newsreader: Trumpet for Windows [Version 1.0 Rev A]

Hello!!

I am a student in Trondheim, Norway who wonder if anyone know how to grow 
Cladonia species in the laboratory. If anyone have heard about 
Lilly & Barnett, 1951(I think this is the writers of an article, or a 
magazine!), it would be fine to send some info over.

Thank you!!

Jonny Tverdal   
Jonnyt@kjemi.tih.no
 
Hilsen Jonny.


From ajwh1@leicester.ac.uk Tue Jan 17 10:11:53 EST 1995
Article: 1316 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!howland.reston.ans.net!agate!biosci!leicester.ac.uk!ajwh1
From: ajwh1@leicester.ac.uk ("A.J.W. Hall")
Newsgroups: bionet.mycology
Subject: Wanted Verticillium
Date: 17 Jan 1995 05:54:46 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 7
Sender: daemon@net.bio.net
Distribution: world
Message-ID: <A3304947B4@rose.le.ac.uk>
NNTP-Posting-Host: net.bio.net

Dear netters,
        Does anybody out there have a culture of verticillium 
agaricinum or V.dahlihae or know where I can get hold of one.
Even better does someone have a DNA prep of the above they
 could send me. If so please e-mail me at AJWH1@Leicester.ac.uk.

Thanks 


From MKOLOTILA@mecn.mass.edu Tue Jan 17 22:28:20 EST 1995
Article: 1317 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!howland.reston.ans.net!agate!biosci!mecn.mass.edu!MKOLOTILA
From: MKOLOTILA@mecn.mass.edu
Newsgroups: bionet.mycology
Subject: chronic candidal toxicity
Date: 17 Jan 1995 09:00:14 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 19
Sender: daemon@net.bio.net
Distribution: world
Message-ID: <01HLYACPU5TE9ZMEG3@mecn.mass.edu>
NNTP-Posting-Host: net.bio.net

Hi;
A friend posted me a question for which I do not have a good answer.  The
question is this:  Are there any scientific publications (either positive or
negative results) for chronic candidal toxicity causing diseases or
dysfunctions, such as autism?  Please post all replies to me.  Thank you in
advance.
Cheers,
Michael
*******************************************************************************
* Michael P. Kolotila, Ph.D.             ** e-mail: mkolotila@mecn.mass.edu   *
* Biotechnology Program Coordinator                                           *
* Biotechnology Program                                                       *
* Department of Natural Science          ** phone:  (508)-374-3887            *
* Northern Essex Community College       ** fax:    (508)-374-3723            *
* 100 Elloitt Way                                                             *
* Haverhill, MA  01830-2399                                                   *
*******************************************************************************
*                Pallidrome:  Have DNA, Will Travel                           *
*******************************************************************************


From JHARRIS@LABA.TDH.TEXAS.GOV Tue Jan 17 22:28:21 EST 1995
Article: 1318 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!howland.reston.ans.net!agate!biosci!LABA.TDH.TEXAS.GOV!JHARRIS
From: JHARRIS@LABA.TDH.TEXAS.GOV ("Jim Harris")
Newsgroups: bionet.mycology
Subject: Medically Important Filamentous Fungi Workshop
Date: 17 Jan 1995 07:56:35 -0800
Organization: Texas Department of Health LABs
Lines: 20
Sender: daemon@net.bio.net
Distribution: world
Message-ID: <1306C596079@laba.tdh.texas.gov>
NNTP-Posting-Host: net.bio.net

Persons interested in attending a two day workshop in Austin, Texas 
concerning medically important filamentous fungi are encouraged to contact 
me at the address below immediately.  The cost of this workshop is $130.  
Date for workshop is February 27-28, 1995. Participants earn 1.2 P.A.C.E. 
credits through ASCLS.  

Phone, FAX or e-mail any questions you may have. Space is limited to 16 
persons.  

Jim Harris, Ph.D.
Training Coordinator
Bureau of Laboratories
Texas Department of Health
phone 512-458-7566
FAX 512-458-7672
e-mail jharris@laba.tdh.texas.gov



jharris@LABA.tdh.texas.gov


From jbrown@bbsrc.ac.uk Tue Jan 17 22:28:22 EST 1995
Article: 1319 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!howland.reston.ans.net!Germany.EU.net!EU.net!uknet!bcc.ac.uk!is.bbsrc.ac.uk!news
From: jbrown@bbsrc (James Brown)
Newsgroups: bionet.mycology,bionet.plants
Subject: RUST-MILDEW: a new Bionet list for cereal pathology
Date: 17 Jan 1995 18:42:07 GMT
Organization: John Innes Centre
Lines: 58
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Reply-To: jbrown@bbsrc.ac.uk
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Mime-Version: 1.0
Content-Type: Text/Plain
X-Newsreader: WinVN 0.92.1
Xref: bigblue.oit.unc.edu bionet.mycology:1319 bionet.plants:4761

A new Bionet mailing list on rust and mildew diseases of cereals has
been set up. A short description of the scope of the list is:
"Biotrophic foliar fungal diseases (rusts and mildews) of cereals".

A longer description is:
"The RUST-MILDEW mailing list concerns the biotrophic foliar fungal
diseases of cereals, including rusts, powdery mildews and downy
mildews. The list focuses on diseases of crops, including wheat,
barley, oats, rye, maize, sorghum, millet, rice and minor cereals, but
can include discussion of diseases of related wild species. Discussion
of all aspects of these diseases is welcome, including genetics,
molecular biology, biochemistry, physiology, taxonomy, evolution,
population genetics and epidemiology of the fungus, all aspects of
host resistance (e.g. genetics, molecular biology, cell biology,
physiology, microscopy), control by fungicides, fungicide resistance,
variety mixtures, agronomy and plant breeding, and any other topic
relevant to these diseases. The mailing list will provide a forum for
discussion of ideas, problems, recent developments and techniques, a
means of sharing expertise and biological materials, and a forum for
announcement of meetings and job opportunities."

Subscribing to the RUST-MILDEW list
-----------------------------------

If you would like to subscribe to the RUST-MILDEW list, send a mail
message to the Internet address

biosci-server@net.bio.net

and enter the following line into the body of the mail message:

subscribe rust-mil

This message will be automatically read by the Bionet computer and
your e-mail address will be extracted from the mail header and added
to the list. Note that sending subscribe/unsubscribe messages to the
other bionet server, mxt@dl.ac.uk, won't work, since new lists on a
6-month trial are only maintained by biosci-server@net.bio.net

How to post a message to the group
----------------------------------

Simply mail your message to the Internet address

rust-mil@net.bio.net

and your message will be distributed automatically to everyone on the
list.  There is no editorial intervention.  PLEASE DO NOT send
subscription requests to this address as you will bother everyone on
the newsgroup.  Please use the biosci-server@net.bio.net address as
instructed above for subscription/cancellation requests.

Finally, please forward this message to anyone you know who might be
interested.

James K.M. Brown
John Innes Centre, Norwich, England
jbrown@bbsrc.ac.uk


From stamets1@aol.com Tue Jan 17 22:28:23 EST 1995
Article: 1320 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!swiss.ans.net!newstf01.news.aol.com!not-for-mail
From: stamets1@aol.com (Stamets 1)
Newsgroups: bionet.mycology
Subject: Where is Dale Leslie ???
Date: 17 Jan 1995 17:07:47 -0500
Organization: America Online, Inc. (1-800-827-6364)
Lines: 7
Sender: root@newsbf02.news.aol.com
Message-ID: <3fhf3j$kse@newsbf02.news.aol.com>
Reply-To: stamets1@aol.com (Stamets 1)

Do any of you know where Dale Leslie, formerly from Los Gatos, California
is and how I can get ahold of him?

Any information would be appreciated.

Paul Stamets



From epichloe@TANGO.AUM.EDU Tue Jan 17 22:28:24 EST 1995
Article: 1321 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!howland.reston.ans.net!spool.mu.edu!olivea!biosci!TANGO.AUM.EDU!epichloe
From: epichloe@TANGO.AUM.EDU
Newsgroups: bionet.mycology
Subject: symbiosis newsgroup
Date: 17 Jan 1995 11:14:17 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 18
Sender: daemon@net.bio.net
Distribution: world
Message-ID: <Pine.LNX.3.91.950117125919.2727A-100000@tango.aum.edu>
NNTP-Posting-Host: net.bio.net


	A new prototype newsgroup has been created for researchers 
interested in symbiotic associations.  This newsgroup may provide an 
alternative forum for mycologists whose research involves symbiotic 
systems.  

	To subscribe to this prototype newsgroup send an e-mail message 
to <biosci-server@net.bio.net>.  In the body of the mail message write: 

subscribe symbios

	For additional information contact:

Jim White
Dept. Biol.
Auburn Univ. at Montgomery
email: epichloe@tango.aum.edu



From ksanders@ccinet.ab.ca Wed Jan 18 23:36:30 EST 1995
Article: 1322 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!howland.reston.ans.net!vixen.cso.uiuc.edu!uwm.edu!news.doit.wisc.edu!decwrl!tribune.usask.ca!quartz.ucs.ualberta.ca!finzi.ccinet.ab.ca!news@ccinet.ab.ca
From: ksanders@ccinet.ab.ca (Ken Sanderson)
Newsgroups: bionet.mycology
Subject: Questions
Date: 18 Jan 1995 05:52:27 GMT
Organization: The Job Track
Lines: 14
Message-ID: <3fiaar$3qm@finzi.ccinet.ab.ca>
NNTP-Posting-Host: fortmacr2.ccinet.ab.ca
X-Newsreader: WinVN 0.90.3

I have a few questions regarding the birch polypore - 
Piptoporus betulinus.  Does anyone know of the culinary and medicinal 
qualities.  If anyone has the book "One thousand American Fungi" by 
Charles McIlvaine, there is supposed to be some information regarding
their edibility.  Is this true, what does it say?  Paul Stamets book 
"Growing Gourmet and Medicinal Mushrooms" mentions that the iceman had 
birch polypores on him and used them for treating wounds and making a
tea by boiling the mushroom.  Does anyone have any other information on
this? Is there a reference in Paul Stamets book to where the information 
came from (I do not have the book)?  Is there any other information anyone
can give me?

Thanks in advance
Ken Sanderson


From rarnold@teleport.com Wed Jan 18 23:36:31 EST 1995
Article: 1323 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!rutgers!csn!yuma!purdue!gatech!howland.reston.ans.net!news.cac.psu.edu!news.pop.psu.edu!hudson.lm.com!godot.cc.duq.edu!ddsw1!news.structured.net!news.teleport.com!usenet
From: "Ralph D. Arnold" <rarnold@teleport.com>
Newsgroups: bionet.mycology
Subject: First Month of FUNGUS archived
Date: Tue, 17 Jan 95 20:16:42 PST
Organization: Teleport - Portland's Public Access (503) 220-1016
Lines: 119
Message-ID: <83125.rarnold@teleport.com>
Reply-To: <rarnold@teleport.com>
NNTP-Posting-Host: ip-pdx2-06.teleport.com
X-Minuet-Version: Minuet1.0_Beta_17A
X-POPMail-Charset: English

//
For mycologist and others interested in mushroom cultivation, just a
reminder that FUNGUS exists and has been active for a month.  Below
is info about this free email newsletter, including how to subscribe
and where archived files exist.
//
///////////////////////////////
Welcome!  You can now subscribe to FUNGUS, a free Internet mailing list
dedicated to mushroom cultivation (both hobby and commercial) and related
mycology resources.

Unfortunately, archived files of FUNGUS are not now available via MAJORDOMO
(email mailing system).  However, now they are available via anonymous
FTP - see IV. below.

QUICK INDEX OF THIS INFO MESSAGE:

I. Philosophical Statement about FUNGUS
II.  Important Non-Internet Resources for the Mushroom Grower
III. Suggestions on What to Submit to FUNGUS
IV. Other Internet Sites Where This Info Is Located
V. Various "Majordomo" Mailing List Commands
  A. How to Subscript to FUNGUS
  B. How to Unsubscribe to FUNGUS
  C. How to Send Messages/Textfiles to FUNGUS
VI. Information on the Moderator of FUNGUS
===============
I. Philosophical Statement about FUNGUS
FUNGUS has been designed as a vehicle for mushroom growers (both hobbyists
and professionals) and interested parties can have one more Internet access
point, for dissemination of information and for discussions relating to
the cultivation of edible and other fungi.  Conceiveable, there will be
a variety of commercial entities publicized and involved.  This is meant to
be a practical approach to cultivation.

II.  Important Non-Internet Resources for the Mushroom Grower
This FREE Internet tool is IN NO WAY meant to replace/supplant other
resources.  I suggest all growers utilize this partial list of resources
<please let me know of add'l resources I should include)

BOOKS:
There are many fine mushroom cultivation books.  In the opinion of the 
moderator, all growers should own "Growing Gourmet & Medicinal Mushrooms"
by Paul Stamets (c) 1993 Ten Speed Press ISBN 0-89815-608-4

MAGAZINES\NEWSLETTERS

Cultivated Mushroom Report, U of Toronto, Mississauga, Ontario, Canada

the Mushroom Growers' Newsletter, mycoworld@aol.com, 464 Fulton St,
   Klamath Falls, OR   97601

Mushroom - the Journal, PO Box 3156, Moscow, ID  83843

Mushroom News, c/o American Mushroom Institute, 907 East Baltimore Pike,
   Kennett Square, PA   19348

Shiitake News, Forest Resource Center, Rt2 Box 156A, Lanesboro, MN  55949

ORGANIZATIONS/ASSOCIATIONS/SEMINARS
These are too numerous to include now.  Hopefully, each portion of every 
catagory will be "advertised" or announced in messages to FUNGUS

III. Suggestions on What to Submit to FUNGUS
Any and all types of information relating to mushroom cultivation, 
especially that relating to non-White Button Mushroom species.
These could include (but are not limited to):
Info on Growing Specific Species
Info on Cultivation Techniques
Indexs to Cultivation Books
Surveys of Literature
Announcements/Details on Associations & Societies with Cultivation Aspects
Announcements/Details on Seminars & Training Opportunities
Mycology Supply Companies
Internet Info Sources
Culture Collection Libraries
Equipment Details and Uses
Questions about Problems Encountered followed by (hopefully) Solutions 
       found by other growers
**please help make FUNGUS applicable and important to your needs and 
interests**

IV. Other Internet Sites Where This Info Is Located

I have setup a GOPHERSPACE where much of this info will be stored.
Access it :

VIA WWW at gopher://gopher.teleport.com/11/users/rarnold

VIA Gopher  gopher.teleport.com  :select "users"; then "on-line access";
                         "fungi-by-email"

I have also set up an anonymous FTP site containing these files. In 
addition, ARCHIVED messages/textfiles will be stored in this area.

VIA FTP at ftp.teleport.com in directory \users\rarnold\mushroom
archived files are in directory \users\rarnold\mushroom\FUNGUS

V. Various "Majordomo" Mailing List Commands
  A. How to Subscribe to FUNGUS
Send an email message to fungus-request@teleport.com with this message:
SUBSCRIBE FUNGUS <your individual email address>
  B. How to Unsubscribe to FUNGUS
Send an email message to fungus-request@teleport.com with this message:
UNSUBSCRIBE FUNGUS <your email address>
  C. How to Send Messages/Textfiles to FUNGUS
Send the message\textfile to fungus@teleport.com.  It will be forwarded to
the moderator (rarnold@teleport.com) who will undoubtedly okay it's
publication.  The rare non-inclusive messages most likely will be 
"subscribe" or "unsubscribe" messages (+see above points to do it right+) 
 and textfiles/messages dealing in illegal matters/subjects.  Discretion
is important.

VI.  Any questions/comments can be directed to the moderator, which is
Ralph D. Arnold.  He can be reached at rarnold@teleport.com.


------ Forwarded message ends here ------



From mycochef@aol.com Wed Jan 18 23:36:33 EST 1995
Article: 1324 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!swiss.ans.net!newstf01.news.aol.com!not-for-mail
From: mycochef@aol.com (MYCOCHEF)
Newsgroups: bionet.mycology
Subject: Re: Questions
Date: 18 Jan 1995 17:09:33 -0500
Organization: America Online, Inc. (1-800-827-6364)
Lines: 6
Sender: root@newsbf02.news.aol.com
Message-ID: <3fk3it$3ou@newsbf02.news.aol.com>
References: <3fiaar$3qm@finzi.ccinet.ab.ca>
Reply-To: mycochef@aol.com (MYCOCHEF)

HELLO,  CAPT. MCILVAINE STATED, "WHEN YOUNG  IT IS EATEN BY DEER.  DRIED
IT BURNS WITH A WHITE FLAME, OR HOLDS FIRE AS WELL AS THE BEST PUNK.  IT
IS A VALUABLE FUEL, ALREADY PREPARED FOR THE STOVE. . . .WHEN VERY YOUNG
IT IS FAIR.  UNPLEASANT WHEN OLD."   REMEMBER  THIS GUY ATE MANY MUSHROOMS
THAT NEITHER MYSELF, NOR ANY OTHER "SOMEWHAT" RESPECTABLE MUSHROOM CHEF
WOULD SERVE.  THOUGH I HAVE EATEN SOME FAIRLY BIZARRE FUNGUS. . . .


From mycochef@aol.com Thu Jan 19 09:37:04 EST 1995
Article: 1325 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!swiss.ans.net!newstf01.news.aol.com!not-for-mail
From: mycochef@aol.com (MYCOCHEF)
Newsgroups: bionet.mycology
Subject: MUSHROOM COOKING
Date: 18 Jan 1995 16:55:07 -0500
Organization: America Online, Inc. (1-800-827-6364)
Lines: 4
Sender: root@newsbf02.news.aol.com
Message-ID: <3fk2nr$3kq@newsbf02.news.aol.com>
Reply-To: mycochef@aol.com (MYCOCHEF)

I AM A MUSHROOM CHEF LIVING IN MARIN COUNTY, CA. AND AM INTERESTED IN
TALKING TO OTHERS FROM THIS, OR ANY AREA.  I COOK FOR DAVID ARORA'S FORAYS
THROUGHOUT THE WEST AND HUNT REGULARLY.  I AM, PROFESSIONALLY, A FOOD AND
RESTAURANT CONSULTANT ALWAYS LOOKING FOR NEW PROJECTS.   


From gren@gram.demon.co.uk Fri Jan 27 12:13:03 EST 1995
Article: 1329 of bionet.mycology
Newsgroups: bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!howland.reston.ans.net!pipex!peernews.demon.co.uk!demon!gram.demon.co.uk!user
From: gren@gram.demon.co.uk (Gareth Renowden)
Subject: Re: Questions
X-Nntp-Posting-Host: gram.demon.co.uk
Message-ID: <gren-2001951806580001@gram.demon.co.uk>
Sender: news@demon.co.uk (Usenet Administration)
Organization: Publishing consultant
References: <3fiaar$3qm@finzi.ccinet.ab.ca>
Date: Fri, 20 Jan 1995 18:06:58 GMT
Lines: 19

In article <3fiaar$3qm@finzi.ccinet.ab.ca>, ksanders@ccinet.ab.ca (Ken
Sanderson) wrote:

> I have a few questions regarding the birch polypore - 
> Piptoporus betulinus. (...) Is there any other information anyone
> can give me?

Here in Britain, P. betulinus is said to have been used as a strop for
sharpening knives. But perhaps ours is tougher than yours.. I certainly
wouldn't countenance eating it, just as I don't eat leather.

(And can MYCOCHEF STOP SHOUTING!)

-- 
Gareth Renowden                      l
Publishing & marketing consultant    l  "I don't know what I want,
Voice +44 (0)181 241 4852            l     but I want it NOW!"
Fax +44 (0)181 943 4294              l    Sir Henry Rawlinson
e-mail gren@gram.demon.co.uk         l     (c) Viv Stanshall


From C_BETANCOURT@RUMAC.UPR.CLU.EDU Fri Jan 27 12:13:04 EST 1995
Article: 1330 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!newsfeed.pitt.edu!hudson.lm.com!news.pop.psu.edu!news.cac.psu.edu!howland.reston.ans.net!spool.mu.edu!olivea!biosci!RUMAC.UPR.CLU.EDU!C_BETANCOURT
From: C_BETANCOURT@RUMAC.UPR.CLU.EDU
Newsgroups: bionet.mycology
Subject: Mold contamination in airplanes?
Date: 20 Jan 1995 12:43:26 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 6
Sender: daemon@net.bio.net
Distribution: world
Message-ID: <950120164540.20200b03@rumac.upr.clu.edu>
NNTP-Posting-Host: net.bio.net

I will like to know is somebody is working with mold contamination in
airplanes or papers related to the topic.  Thanks for the help.


						Carlos Betancourt
						University of Puerto Rico


From JHARRIS@LABA.TDH.TEXAS.GOV Fri Jan 27 12:13:05 EST 1995
Article: 1331 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!howland.reston.ans.net!spool.mu.edu!olivea!biosci!LABA.TDH.TEXAS.GOV!JHARRIS
From: JHARRIS@LABA.TDH.TEXAS.GOV ("Jim Harris")
Newsgroups: bionet.mycology
Subject: pre-MMSA-ASM Workshop
Date: 20 Jan 1995 14:06:49 -0800
Organization: Texas Department of Health LABs
Lines: 18
Sender: daemon@net.bio.net
Distribution: world
Message-ID: <17E9B240863@laba.tdh.texas.gov>
NNTP-Posting-Host: net.bio.net

The Texas Department of Health Bureau of Laboratories and the National 
Laboratory Training Network will again cosponsor a special mycology 
workshop prior to the national meetings of MMSA and ASM.  The workshop is 
not affiliated with either society but will be scheduled on May 18-19, 
1995, prior to the society meetings.
The program is "Fungi to be Feared?: Condo Contaminants and Toxic 
Toadstools."  Topics will range from identification of macro and 
microfungi to the significance of toxins from these organisms to 
psychosomatic aspects of mold toxicosis, specifically "sick building 
syndrome."  Further details on site (in Washington D.C.), fees, speakers, 
etc may be obtained by e-mail reply to this message or calling

Jim Harris, Ph.D.
Training Coordinator
Bureau of Laboratories
Texas Dept of Health
512-458-7566
jharris@LABA.tdh.texas.gov


From unicornbag@aol.com Fri Jan 27 12:13:06 EST 1995
Article: 1332 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!swrinde!pipex!uunet!newstf01.news.aol.com!not-for-mail
From: unicornbag@aol.com (UNICORNbag)
Newsgroups: bionet.mycology
Subject: MUSHROOM IMPORTS FROM CHINA
Date: 20 Jan 1995 19:04:11 -0500
Organization: America Online, Inc. (1-800-827-6364)
Lines: 5
Sender: root@newsbf02.news.aol.com
Message-ID: <3fpj1r$dh@newsbf02.news.aol.com>
Reply-To: unicornbag@aol.com (UNICORNbag)

Does anyone know if there has been imports of shiitake mushrooms from 
China ? into the US or Canada, names of importers ? quality ? Shelf Life ?
prices ? and any other information please
Fax 1-800 797 2247
UNICORNbag@aol.com(L.Hsu)


From unicornbag@aol.com Fri Jan 27 12:13:07 EST 1995
Article: 1333 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!swrinde!pipex!uunet!newstf01.news.aol.com!not-for-mail
From: unicornbag@aol.com (UNICORNbag)
Newsgroups: bionet.mycology
Subject: Mushroom shelf life and irradiation
Date: 20 Jan 1995 19:07:12 -0500
Organization: America Online, Inc. (1-800-827-6364)
Lines: 7
Sender: root@newsbf02.news.aol.com
Message-ID: <3fpj7g$em@newsbf02.news.aol.com>
Reply-To: unicornbag@aol.com (UNICORNbag)

Can anyone give me some leads on irradiation and mushroom shelf life
especially
regarding shiitake and pleurotus mushrooms.

Fax 1 800 797 2247

UNICORNbag@aol.com


From ez024693@dale.ucdavis.edu Fri Jan 27 12:13:08 EST 1995
Article: 1334 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!paladin.american.edu!howland.reston.ans.net!agate!news.ucdavis.edu!dale!ez024693
From: ez024693@dale.ucdavis.edu (James Salyards)
Newsgroups: bionet.mycology
Subject: Black Bolete
Date: 21 Jan 1995 00:42:44 GMT
Organization: University of California, Davis
Lines: 4
Message-ID: <3fpla4$pb8@mark.ucdavis.edu>
NNTP-Posting-Host: dale.ucdavis.edu
Summary: ID question
Keywords: Black Bolete
X-Newsreader: TIN [version 1.2 PL2]

My local coop sells packages of dried mushrooms labeled- black boletes.  
I wonder if anyone knows which species this is?  The mushrooms are darker 
and more fragrant than the Boletus edulis mushrooms they sell, and their 
cheaper too.  Please let me know.  


From Gregory.Russell@MED.UMICH.EDU Fri Jan 27 12:13:09 EST 1995
Article: 1335 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!rutgers!gatech!howland.reston.ans.net!spool.mu.edu!olivea!biosci!MED.UMICH.EDU!Gregory.Russell
From: Gregory.Russell@MED.UMICH.EDU ("Gregory Russell")
Newsgroups: bionet.mycology
Subject: ANTIPARASITE DRUG SCREENING
Date: 20 Jan 1995 11:20:51 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 25
Sender: daemon@net.bio.net
Distribution: world
Message-ID: <n1421510854.28089@mailgw.surg.med.umich.edu>
NNTP-Posting-Host: net.bio.net

  REGARDING           ANTIPARASITE DRUG SCREENING

Dear Biosci Researchers,
I am considering establishing a small biotech company to screen for
antiparasitic agents using compounds which have displayed little or no
activity in vertebrate or mammalian assay systems. I also wish to develop new
products based upon peptides or natural products in conjunction with mutation
and molecular techniques. The initial assay system has been developed for the
rapid screening of compounds displaying anthelmintic activity. It is my desire
to expand and develop this technology to rapidly identify active antiparasitic
drugs that display little or no host toxicity. The animal health industry is
the target market, however it is likely that a product suitable for the human
market may result from this work. The major focus will be on anthelmintic,
antifilarial, antiprotozoal and antifungal drug discovery.
I seek interested parties from either industry or academia for collaboration
or partnership in this enterprise. A cooperative research agreement with an
established pharmaceutical or biotech company would probably be the best
begining of this project. If you are interested in this concept please contact
me at grussell@umich.edu.
Thankyou,
Greg Russell Ph.D.
Research Fellow.





From staben@seqanal.mi.uky.edu Fri Jan 27 12:13:11 EST 1995
Article: 1336 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!europa.eng.gtefsd.com!news.umbc.edu!cs.umd.edu!newsfeed.gsfc.nasa.gov!ukma!seqanal.mi.uky.edu!staben
From: staben@seqanal.mi.uky.edu (Chuck Staben)
Newsgroups: bionet.mycology
Subject: codon usage in N. crassa
Date: 19 Jan 1995 23:35:06 GMT
Organization: University of Kentucky, Dept. of Math Sciences
Lines: 6
Message-ID: <3fmsva$lj4@t2.mscf.uky.edu>
NNTP-Posting-Host: seqanal.mi.uky.edu
X-Newsreader: TIN [version 1.1 PL9]

Experimental Mycology 18:70-81 contains a codon usage table and other data
concerning Neurorospora genes.  Similar data for a variety of organisms
have also been annually collected in an issue of Nucleic Acids Research.
Our paper (above) suggests that usage statistics are unlikely to be 
dramatically altered by the inclusion of additional data, so you can feel
fairly comfortable with our table, although it contains about 80 genes.


From kasplash@crl.com Fri Jan 27 12:13:12 EST 1995
Article: 1337 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!howland.reston.ans.net!nntp.crl.com!crl12.crl.com!not-for-mail
From: kasplash@crl.com (Bob Galvan)
Newsgroups: bionet.mycology
Subject: Portobello
Date: 20 Jan 1995 17:48:00 -0800
Organization: CRL Dialup Internet Access	(415) 705-6060  [login: guest]
Lines: 10
Message-ID: <3fpp4g$pdb@crl12.crl.com>
References: <3fmsva$lj4@t2.mscf.uky.edu>
NNTP-Posting-Host: crl12.crl.com

recently  a large brown mushroom has appeared in Marin and San Francisco 
markets under the name "Portobello".  At my novice level of 
identification skills, I can only say that it looks like Agaricus bitorquis.

Does anyone know  what this 'shroom really is?   

Bob Galvan
kasplash@crl.com




From mspear@telerama.lm.com Fri Jan 27 12:13:13 EST 1995
Article: 1338 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!newsfeed.pitt.edu!hudson.lm.com!terrazzo.lm.com!not-for-mail
From: mspear@telerama.lm.com (mspear)
Newsgroups: bionet.mycology
Subject: Re: Portobello
Date: 20 Jan 1995 21:08:15 -0500
Organization: Telerama Public Access Internet, Pittsburgh, PA
Lines: 34
Message-ID: <3fpqaf$419@terrazzo.lm.com>
References: <3fmsva$lj4@t2.mscf.uky.edu> <3fpp4g$pdb@crl12.crl.com>
NNTP-Posting-Host: terrazzo.lm.com
X-Newsreader: TIN [version 1.2 PL2]

Bob Galvan (kasplash@crl.com) wrote:
> recently  a large brown mushroom has appeared in Marin and San Francisco 
> markets under the name "Portobello".  At my novice level of 
> identification skills, I can only say that it looks like Agaricus bitorquis.

> Does anyone know  what this 'shroom really is?   

> Bob Galvan
> kasplash@crl.com


This is our old friend _Agaricus_brunnescense_ (a.k.a. bisporus) var
bisporus.  Yes, the new "wild" taste treat is in fact the same old
common champignon du paris - white button mushroom we all know well. 
Here's what happened:

Growers figured out that they can use a brown colored variety of
mushroom and let them "overmature" until they open, flatten and the
spores mature.  The costs of production are much lower since picking
costs go way down.  The price can be doubled.

On the positive side most people feel that a mature mushroom is much
more flavorful than the closed buttons Americans normally eat.  I feel
the need to have a super-white button is part of the
white-bread-sydrome.  No flavor but looks nice.

On the negative side for growers, however, is the greatly increased
risk of the farms getting a disease call "La France" virus.  This is
totally harmless to humans but can wipe out a mushroom farm.

Portobello's are called "opens" or "flats" in England and Australia
where they have been a staple of the market for decades.  In these
countries you can get a nice breakfast flat fried with egg or breaded
for breakfast.  It's great!


From mspear@telerama.lm.com Fri Jan 27 12:13:14 EST 1995
Article: 1339 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!news-server.ncren.net!taco.cc.ncsu.edu!gatech!howland.reston.ans.net!news.cac.psu.edu!news.pop.psu.edu!hudson.lm.com!africa.lm.com!not-for-mail
From: mspear@telerama.lm.com (mspear)
Newsgroups: bionet.mycology
Subject: Dr. Sinden Dead
Date: 21 Jan 1995 23:13:06 -0500
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Since this newsgroup is into myco-culture perhaps the membership would
want to learn of the passing of the great Dr. James Sinden. 

Sinden entered the button mushroom industry in the 1940s.  At that
time the Agaricus industry is where the Exotics industry is now. 
Sinden did a lot including the invention of grain spawn, the short
composting method, growing in trays, and other innovations we take
for granted nowdays.

Sinden lived past 90 to see acclaim from those who knew his
accomplishments.  There are not many like Doc.


From VPGH51A@prodigy.com Fri Jan 27 12:13:15 EST 1995
Article: 1340 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!swiss.ans.net!prodigy.com!usenet
From: VPGH51A@prodigy.com (Jim Berlstein)
Newsgroups: bionet.mycology
Subject: Re: Black Bolete
Date: 23 Jan 1995 03:24:05 GMT
Organization: Prodigy Services Company  1-800-PRODIGY
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Just a guess, but I would guess the black bolete could be Leccinum 
Manzanita, or the Manzanita Bolete.  It stains Blackish/bluish/purplish 
whaen cooked are dried.  Like all leccinums, it should have raised dark 
spots on the stem, unlike the reticulations on the stalk of the Boletus 
Edulis.
       They also sell for less, and are less flavorful.  I saw one 
Boletus Edulis atop a pile of Manzanita Boletes at Pike street market in 
Seattle, the whole bin labeled as Boletus Edulis.  I confronted the owner 
of the stand, who clearly was a victim, as well as an unwitting 
perpetrator of fraud.  My wife called me off of him.  Of course, he 
should be following the basic rule of....

If you can't identify it, don't sell it!

Of course, I could be wrong.

Jim Berlstein
Waiting for Autumn




From mh@SARG.PGH.WEC.COM Fri Jan 27 12:13:16 EST 1995
Article: 1341 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!howland.reston.ans.net!spool.mu.edu!olivea!biosci!SARG.PGH.WEC.COM!mh
From: mh@SARG.PGH.WEC.COM ("Mike Hoffelder")
Newsgroups: bionet.mycology
Subject: Morel Production
Date: 23 Jan 1995 08:08:46 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
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I saw a PBS show that showed researchers at Michigan State (I believe)
who were developing a process for commercial production of Morels.
Does anybody know more about this or who to contact?


From gren@gram.demon.co.uk Fri Jan 27 12:13:17 EST 1995
Article: 1342 of bionet.mycology
Newsgroups: bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!swrinde!pipex!peernews.demon.co.uk!demon!gram.demon.co.uk!user
From: gren@gram.demon.co.uk (Gareth Renowden)
Subject: Edible fungi in New Zealand
X-Nntp-Posting-Host: gram.demon.co.uk
Message-ID: <gren-2401951026280001@gram.demon.co.uk>
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Date: Tue, 24 Jan 1995 10:26:28 GMT
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I shall be moving to New Zealand in the next year or so, and as keen
amateur mushroom hunter and eater am naturally interested in what can be
found and eaten in that fair land. Any pointers/good books etc? All help
gratefully received.

-- 
Gareth Renowden                      l
Publishing & marketing consultant    l  "I don't know what I want,
Voice +44 (0)181 241 4852            l     but I want it NOW!"
Fax +44 (0)181 943 4294              l    Sir Henry Rawlinson
e-mail gren@gram.demon.co.uk         l     (c) Viv Stanshall


From staben@ukcc.uky.edu Fri Jan 27 12:13:18 EST 1995
Article: 1343 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!news-server.ncren.net!taco.cc.ncsu.edu!gatech!news-feed-1.peachnet.edu!ukma!news.uky.edu!chuck
From: Chuck Staben <staben@ukcc.uky.edu>
Newsgroups: bionet.mycology
Subject: electroporation
Date: 23 Jan 1995 18:42:28 GMT
Organization: University of Kentucky Computing Services
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NNTP-Posting-Host: 128.163.21.206

I would like to develop an electroporation protocol for Aspergillus nidulans.
I am aware of conditions for N. crassa and for S. cerevisiae, and I know
that many other fungi have been electroporated.
I would like to hear other people's experience with Aspergillus or
general guidance on developing an effective electroporation protocol.
Mark Lies
Please reply to:
cmlies00@ukcc.uky.edu


From cfaw@cabrach.demon.co.uk Fri Jan 27 12:13:20 EST 1995
Article: 1344 of bionet.mycology
Newsgroups: bionet.agroforestry,bionet.mycology,eunet.news
From: cfaw@cabrach.demon.co.uk (Francis A Williamson)
Path: bigblue.oit.unc.edu!concert!gatech!swrinde!pipex!peernews.demon.co.uk!cabrach.demon.co.uk!cfaw
Subject: SME Partners Sought
Organization: Myorganisation
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Sender: usenet@demon.co.uk
Xref: bigblue.oit.unc.edu bionet.agroforestry:1087 bionet.mycology:1344

We are developing a proposal for EU funding for forestry/mycorrhiza 
research.

We need collaboration from SMEs in EU countries (non-UK).

Having failed to gain immediate mastery of the CORDIS
database, we use this forum to ask all non-UK SMEs with interests
in forestry and/or mycorrhizas to contact us as soon as possible
with proposals for collaboration (email, fax, telephone or post).

Any suggestions of hot topics from non-SMEs would of course
be greatly appreciated too.
-- 
Francis A Williamson, PhD
Williamson Research Ltd
Lesmurdie House
Lower Cabrach
Huntly AB54 4EU
Scotland, UK

Tel/Fax +(44) 466 702236
email: cfaw@cabrach.demon.co.uk


From stewart@bud.peinet.pe.ca Fri Jan 27 12:13:21 EST 1995
Article: 1345 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!howland.reston.ans.net!spool.mu.edu!torn!news.unb.ca!peinet.pe.ca!peinet.pe.ca!not-for-mail
From: stewart@bud.peinet.pe.ca (Paul Stewart)
Newsgroups: bionet.mycology
Subject: mycorhizal fungi
Date: 25 Jan 1995 09:33:18 -0400
Organization: PEINet, Charlottetown, Prince Edward Island, Canada
Lines: 21
Sender: stewart@bud.peinet.pe.ca <Paul Stewart>
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Keywords: boletes, chanterelle, mycorhizal

Fellow Myconuts:
	Has anyone a contact for research or info regarding mycorhizal fungi?
Specifically, I'm looking for any attempts to culture them.  My idea is 
to try extracts of the companion roots or surrounding soil as additives 
to agar and similar additives to the spawn mix (grain, wood chips, sawdust).
	I have successfully grown mycelium cultures of Boletus edulis 
(king bolete), chanterelles, and Suillus luteus (slippery Jack), all of 
which are native to PEI, but not reliably plentiful enough to wild 
harvest (not crazy about wild harvest anyway).  I've heard from plenty of 
people that I'm wasting my time, so I'd rather that not be my only reply 
(Stamets' latest book seems to agree with me).
	Anyone with ideas to share please e-mail me or reply to this 
group.  I'll continue to be willing to share any of my own findings.  I 
have already identified some necessary trace elements from soil 
containing Chanterelle mycelia.  I just took samples from the mycelial 
layer, the grass root zone, and below this, followed by similar samples a 
few feet away.  The mycelia seemed to accumulate manganese, boron, and 
calcium in significant quantities.  Any comments.....?
					Paul Stewart




From SEIFERTK@NCCCOT.AGR.CA Fri Jan 27 12:13:22 EST 1995
Article: 1346 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!howland.reston.ans.net!spool.mu.edu!olivea!biosci!NCCCOT.AGR.CA!SEIFERTK
From: SEIFERTK@NCCCOT.AGR.CA
Newsgroups: bionet.mycology
Subject: Mycorrhizal fungi mailing list
Date: 25 Jan 1995 08:58:20 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
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The recent post from the person looking for information
on culturing mycorrhizal fungi might have more luck
going to the Micronet mailing list at the University
of Guelph.  This is a mailing list for mycorrhizal
researchers, but is not part of the bio.net system.


To join: Send the following message to listserv@uoguelph.ca 
subscribe micronet

For information:  contact Dr. Lewis Melville at lmelvill@uoguelph.ca 

Keith Seifert
Agriculture and Agri-Food Canada
Ottawa
seifertk@ncccot.agr.ca


From caddick@liverpool.ac.uk Fri Jan 27 12:13:23 EST 1995
Article: 1347 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!hearst.acc.Virginia.EDU!portal.gmu.edu!europa.eng.gtefsd.com!usenet.eel.ufl.edu!news.bluesky.net!udel!news.sprintlink.net!EU.net!uknet!daresbury!not-for-mail
From: "Dr. M. Caddick" <caddick@liverpool.ac.uk>
Newsgroups: bionet.mycology
Subject: EMBO Workshop 1995
Date: 25 Jan 1995 18:11:33 -0000
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EMBO workshop

         Molecular Biology of Filamentous Fungi
      "Genome structure, maintenance and expression"
     
       Ambleside, U.K.  2nd - 7th September,1995

Opening Lecture - David Perkins

DNA repair and recombination      Meiosis and the Cell Cycle
Peter Strike, UK                  Denise Zickler,France
Anthony Carr, UK                  John Doonan, UK
Henk van den Broek, Netherlands   Steve Osmani,USA
Laurent Maloisel, France          John Hamer,USA
Alain Nicolas, France             Oded Yarden,Israel
Hirokazu Inoue, Japan             Reinhart Fischer, Germany

Genome organisation and           Signal transduction and the co-
expression                        ordination of gene expression
Lorna Casselton, UK
John Taylor, USA                  Joan Tilburn, UK
Marie-Josee Daboussi, France      Miguel Penalva, Spain
Nigel Belshaw, UK                 John Linz, USA
Francesca Palladino, Switzerland  Enzo Russo,Germany
Barbara Valent, USA               Guiseppe Macino, Italy
Claudio Scazzocchio, France       Thomas Adams, USA

Format:  This EMBO funded workshop has a strictly
limited number  of participants, who will be chosen
on the  basis of  scientific  merit  and  current
research  interests. Where possible, we will select
a broad representation  of primarily  European based
scientists ranging from  senior graduate  students
to group leaders.   All  participants will  be
required to present a poster and be willing  to give
a short informal talk relating to their work.

There  is no registration fee, but participants
will  be required to pay some of their accommodation
costs.   Some bursaries   will  be  available  for
junior  scientists, requests  for  which should be
included in  the  original application.

Applications,  consisting of  a  brief  CV  and  a
short description  of  current research interests,
or  further enquires  should be made to Dr Richard
Oliver, School  of Biological Sciences, UEA, Norwich
NR4 7TJ, UK   Fax:   44 603 592250    Tel:44 603 592777.    
E mail:R.oliver@uea.ac.uk.  Applications must be received
by the 28th of February 1995.

Organisers:   Mark  Caddick  and  Richard   Oliver.
Coorganisers:  Henk van den Broek, Claudio Scazzocchio
and Miguel  Penalva. Advisors:  David Archer, Herb Arst  Jr.,
John  Doonan,  Cornelius van den Hondel, Merja  Pentilla, 
Ulf   Stahl,  Peter Strike,  Brian  Tomsett   and   Paul Tudzynski.



From C_BETANCOURT@RUMAC.UPR.CLU.EDU Fri Jan 27 12:13:24 EST 1995
Article: 1348 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!newsxfer.itd.umich.edu!agate!biosci!RUMAC.UPR.CLU.EDU!C_BETANCOURT
From: C_BETANCOURT@RUMAC.UPR.CLU.EDU
Newsgroups: bionet.mycology
Subject: Histoplasma capsulatum in caves?
Date: 26 Jan 1995 04:24:02 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
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I am a graduate student of the University of Puerto Rico at Mayaguez.
I would like to know is somebody is working right now isolating
Histoplasma capsulatum from bat manure from caves.  I will begin my
research at Mona Island's caves in February of this year. 


Also I have another question: Is there another way to isolate this fungus
without the use of laboratory animals? 

					Thanks for the help;
					Angel M. Nieves-Rivera


From mspear@telerama.lm.com Fri Jan 27 12:13:25 EST 1995
Article: 1349 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!news-server.ncren.net!news.duke.edu!godot.cc.duq.edu!hudson.lm.com!asia.lm.com!not-for-mail
From: mspear@telerama.lm.com (mspear)
Newsgroups: bionet.mycology
Subject: Re: Morel Production
Date: 24 Jan 1995 21:36:12 -0500
Organization: Telerama Public Access Internet, Pittsburgh, PA
Lines: 29
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Message-ID: <3g4des$2hr@asia.lm.com>
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X-Newsreader: TIN [version 1.2 PL2]

Mike Hoffelder (mh@SARG.PGH.WEC.COM) wrote:

> I saw a PBS show that showed researchers at Michigan State (I believe)
> who were developing a process for commercial production of Morels.
> Does anybody know more about this or who to contact?
I'm not sure that the University is still doing much work.  Several
years ago the project "went commercial".  First the idea was to sell
licenses but the price was _way_ to high.  As far as I know the only
company who bought in had the backing of the Swiss government and
still was unprofitable.  Next the technology was sold to Domino's
pizza.  That lasted a couple of years.  Finally the whole thing was
sold again.  This time to a company called Terry Foods.  Terry is a
large and respected grower of button mushrooms.

As I understand it there is a pilot plant in place that is rumored to
be pretty nice but not self supporting financially.  Last I heard,
Terry plans to invest enough to make a profitable production plant. 
Domino's promised this as well but reconsidered.

This work has been almost successful for a number of years.  Email me
if you want the contact details.  Remember that the owners of the
process as it stands consider it highly proprietary.  They even have a
patent but many have criticized the patent as being so non-informative
as to be invalid.  In any event they are not about to give away their
technology to casual questioners.  

They may still be looking for investers however.

    ---- Mark               mspear@sylvanres.com


From ad727@rgfn.epcc.Edu Fri Jan 27 12:13:26 EST 1995
Article: 1350 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!howland.reston.ans.net!agate!biosci!RGFN.EPCC.EDU!ad727
From: ad727@RGFN.EPCC.EDU (Lester Pasarell)
Newsgroups: bionet.mycology
Subject: Re: Histoplasma capsulatum in caves?
Date: 26 Jan 1995 06:18:35 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 45
Sender: daemon@net.bio.net
Distribution: world
Message-ID: <9501261418.AA08851@rgfn.epcc.Edu>
Reply-To: ad727@rgfn.epcc.Edu
NNTP-Posting-Host: net.bio.net



Angel M.Nieves-Rivera writes:

>I am a graduate student of the University of Puerto Rico at Mayaguez.
>I would like to know is somebody is working right now isolating
>Histoplasma capsulatum from bat manure from caves.  I will begin my
>research at Mona Island's caves in February of this year. 
>
>
>Also I have another question: Is there another way to isolate this fungus
>without the use of laboratory animals? 
>
>					Thanks for the help;
>					Angel M. Nieves-Rivera
>
>
>
Hi there!! I am not currently working with the isolation of H. capsulatum 
>from  caves, but I do work with the organism from clinical specimens. I assume
that a good medium for the isolation of H capsulatum from contaminated sources
could be a medium that contains cycloheximide with chloramphenicol 
and gentamicin, and maybe incubating it at 35C, this will hopefully inhibit
saprophytic fungi along with bacteria.
Another medium that could be used is Yeast-Extract-Phosphate Agar that uses
ammonium hydroxide, concentrated as the agent to get rid of the contaminants
a detailed recipe for the media above can be obtained from :
  Laboratory Handbook of Medical Mycology by Michael R. McGinnis, 1980
  Academic Press, NY
or if you want me I could fax or mail you the formulation.

One note is that some isolates of H capsulatum may become susceptible to
cycloheximide and chloramphenicol at temperatures above 35C

Hope you have a lot of fun in Mona Island, be careful with the Goats and 
Iguanas!!!

Please let me know if I can be or further help

Lester Pasarell                       E-Mail= ad727@rgfn.epcc.edu
Mycology Research Laboratory
Center for Tropical Diseases
University of Texas Medical Branch
Galveston, Texas 77555-0609
USA


From tjvolk@facstaff.wisc.edu Fri Jan 27 12:13:27 EST 1995
Article: 1351 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!howland.reston.ans.net!news.moneng.mei.com!uwm.edu!news.doit.wisc.edu!F180-040.net.wisc.edu!tjvolk
From: tjvolk@facstaff.wisc.edu (Tom Volk)
Newsgroups: bionet.mycology
Subject: Re: mycorhizal fungi
Date: Thu, 26 Jan 1995 14:42:20 GMT
Organization: U.S. Forest Service
Lines: 18
Distribution: world
Message-ID: <tjvolk.72.2F27B4CB@facstaff.wisc.edu>
References: <3g5juu$8rf@bud.peinet.pe.ca>
NNTP-Posting-Host: f180-040.net.wisc.edu
Keywords: boletes, chanterelle, mycorhizal

Hi Paul.  You wrote:
>Fellow Myconuts:
>        Has anyone a contact for research or info regarding mycorhizal fungi?
>Specifically, I'm looking for any attempts to culture them.  My idea is 
>to try extracts of the companion roots or surrounding soil as additives 
>to agar and similar additives to the spawn mix (grain, wood chips, sawdust).
[some stuff deleted}

For an excellent recnt article see Leonard Hutchison, 1991 Description and 
identification of cultures of ectomycorrhizal fungi found in North America.  
Mycotaxon 42:387-504.  IN this article, Dr. Hutchision describes some 
techniques for isolating & growing cultures of mycorrhizal fungi, and 
characters to look for in the cultres to be sure you have the right fungus 
in culture. note: includes many boletes, but not chanterelles.  INcludes 
many other genera, e.eg Tricholoma, Hebeloma, Amanita, Laccaria, etc..  I 
highly recommend the article.
---Tom Volk  Center for Forest Mycology Research, USDA Forest Products Lab, 
MAdison Wisconsin    <tjvolk@facstaff.wisc.edu>


From rarnold@teleport.com Fri Jan 27 12:13:29 EST 1995
Article: 1352 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!howland.reston.ans.net!vixen.cso.uiuc.edu!news.uoregon.edu!psgrain!rainrgnews0!news.teleport.com!usenet
From: "Ralph D. Arnold" <rarnold@teleport.com>
Newsgroups: bionet.mycology
Subject: Re: Morel Production
Date: Thu, 26 Jan 95 17:12:55 PST
Organization: Teleport - Portland's Public Access (503) 220-1016
Lines: 32
Message-ID: <73089.rarnold@teleport.com>
Reply-To: <rarnold@teleport.com>
NNTP-Posting-Host: ip-pdx4-11.teleport.com
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To: mh@SARG.PGH.WEC.COM

On 23 Jan 1995 08:08:46 -0800, 
"Mike Hoffelder"  <mh@SARG.PGH.WEC.COM> wrote:

>
>I saw a PBS show that showed researchers at Michigan State (I believe)
>who were developing a process for commercial production of Morels.
>Does anybody know more about this or who to contact?
>
There exists 4-5 messages about morel growing (comm'l & hobby//indoors &
outdoors) within the archived files of FUNGUS, the email newsletter on
mushroom growing.  Feel free to retrieve them, if you wish.

BY ANONYMOUS FTP
ftp.teleport.com  in directories \pub\users\rarnold\mushroom\FUNGUS

BY GOPHER
via www gopher://gopher.teleport.com/11/users/rarnold
via gopher   gopher.teleport.com  : select "users"; then "on-line access";
                   then "fungi-by-email"

BY VERONICA
search "Fungi-By-Email"

You can subscribe to FUNGUS by sending to fungus-request@teleport.com
this message:  SUBSCRIBE FUNGUS <you@your.domain>

Good luck!
 

Ralph D. Arnold rarnold@teleport.com 
P.O. Box 889, Oregon City, OR 97045   
fax: 503-423-7409


From wharrison@aol.com Fri Jan 27 12:13:30 EST 1995
Article: 1353 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!swrinde!pipex!uunet!newstf01.news.aol.com!newsbf02.news.aol.com!not-for-mail
From: wharrison@aol.com (WHARRISON)
Newsgroups: bionet.mycology
Subject: Re: Morel Production
Date: 26 Jan 1995 19:11:26 -0500
Organization: America Online, Inc. (1-800-827-6364)
Lines: 10
Sender: root@newsbf02.news.aol.com
Message-ID: <3g9dne$716@newsbf02.news.aol.com>
References: <3g5pne$qae@newsbf02.news.aol.com>
Reply-To: wharrison@aol.com (WHARRISON)
NNTP-Posting-Host: newsbf02.mail.aol.com

There are a couple of articles about the commercial morel facility in
Michigan in "The Best of Spores Afield" available through the internet. 
Point a Gopher at  <gopher.igc.apc.org> and open the "Environment" menu,
then the "Miscellaneous" menu to the "Mycology & Mushrooms" menu.
Wayne Harrison   wharrison@aol.com             
Pine, Colorado      ____________         
elev. 8,500 feet  /  (mushroom)  \
"pray for rain"   \______________/              
                             |    |
                            /      \


From Tom@bio.tamu.edu Fri Jan 27 12:13:31 EST 1995
Article: 1354 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!howland.reston.ans.net!spool.mu.edu!olivea!biosci!BIO.TAMU.EDU!TOM
From: TOM@BIO.TAMU.EDU ("Tom Adams")
Newsgroups: bionet.mycology
Subject: Graduate Fellowships
Date: 26 Jan 1995 12:00:57 -0800
Organization: Texas A&M University - Biology Dept
Lines: 90
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NNTP-Posting-Host: net.bio.net

Program for the Biology of Filamentous Fungi (PBOFF)
Texas A&M University

Graduate Fellowships in Fungal Biology

Fellowships are available for suitably qualified graduate students to work 
with selected members of the Biology, Biochemistry, and Plant Pathology and 
Microbiology Departments at Texas A&M University.  This program is funded by 
a five year training grant from the National Science Foundation and graduate 
trainees will participate in a multidisciplinary program addressing 
important questions in fungal biology.  Students may pursue Ph.D. degrees in 
Plant Pathology, Biology, or Genetics and will have the opportunity to 
rotate in three laboratories before initiating their thesis work in a chosen 
laboratory.  Students with basic interests in biochemistry, biology, 
genetics, and molecular genetics are strongly encouraged to apply.  The 
departmental affiliation and research interests of the faculty associated 
with PBOFF are:

Thomas Adams (Biology/Genetics):  Genetic regulation of fungal development 
and secondary metabolism

David Appel (Plant Pathology):  Ecology and control of major diseases of 
forest and shade  trees, focusing on epidemiology of vascular wilts.

Marian Beremand (Plant Pathology/Genetics):  Genetics and molecular biology 
of mycotoxin production in Fusarium

Daniel Ebbole (Plant Pathology/Genetics):  Genetic regulation of fungal 
development and pathogenicity

Leland Ellis (Biochemistry):  Genome Informatics; developmental genetics of 
kinase structure/function Aspergillus nidulans

Nancy Keller (Plant Pathology):  Genetic and biochemical regulation of 
mycotoxin biosynthesis in Aspergillus

Charles Kenerley (Plant Pathology):  Microbial ecology of biocontrol and 
bioremediation agents

Clint Magill (Plant Pathology/Genetics):  DNA diagnostics of fungal plant 
pathogens and host responses to infection

Raymond Martyn (Plant Pathology):  Biology and control of soilborne fungal 
pathogens

Bruce McDonald (Plant Pathology/Genetics):  Population genetics of plant 
pathogenic fungi/fungal-plant coevolution.

Mike Plamann (Biology):  Genetic analysis of hyphal growth and nuclear 
movement

Neal Van Alfen (Plant Pathology):  Molecular mechanisms of viral repression 
of sporulation and virulence of a fungal plant pathogen

Students interested in entering PBOFF may apply through the Department of 
Plant Pathology and Microbiology, the Biology Department, or the Genetics 
Program.  To insure consideration for receiving funding by PBOFF, we request 
that you state your interest in the program on your application and that you 
also notify us directly in a separate letter to:

Tom Adams
Chairman, Program for Biology of 
Department of Biology
Texas A&M University
Station, TX  77843. 
email: TOM@bio.tamu.edu

Please include in the letter to me information about your areas of interest, 
undergraduate major (and graduate major if you are currently enrolled in 
another program), grade point average, and GRE scores.

To obtain more information about the Program for the Biology of Filamentous 
Fungi at Texas A&M University write:

Program for Biology of Filamentous Fungi
Department of Plant Pathology and Microbiology
Texas A&M University
College Station, TX  77843-2132
Phone:  409-845-7311
FAX:  409-845-6483

OR, take a look at our new World Wide Web page at:

http://straylight.tamu.edu/tamu/pboff/pboff.html
Tom Adams
Department of Biology
Texas A&M University
College Station, TX  77843
409-845-1468
Tom@bio.tamu.edu


From mycochef@aol.com Fri Jan 27 12:13:35 EST 1995
Article: 1355 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!swiss.ans.net!newstf01.news.aol.com!newsbf02.news.aol.com!not-for-mail
From: mycochef@aol.com (MYCOCHEF)
Newsgroups: bionet.mycology
Subject: Re: Questions
Date: 26 Jan 1995 16:43:04 -0500
Organization: America Online, Inc. (1-800-827-6364)
Lines: 4
Sender: root@newsbf02.news.aol.com
Message-ID: <3g9518$588@newsbf02.news.aol.com>
References: <gren-2001951806580001@gram.demon.co.uk>
Reply-To: mycochef@aol.com (MYCOCHEF)
NNTP-Posting-Host: newsbf02.mail.aol.com

sorry old chap, bit of hard cheese you know to be so new and uninformed as
to internet etiquette.  i shall never shout again unless it is in good
taste, and good taste is what i do!  do try and stay away from sour tea.
ta-ta from marin county california.


From mycochef@aol.com Fri Jan 27 12:13:36 EST 1995
Article: 1356 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!howland.reston.ans.net!swiss.ans.net!newstf01.news.aol.com!newsbf02.news.aol.com!not-for-mail
From: mycochef@aol.com (MYCOCHEF)
Newsgroups: bionet.mycology
Subject: Re: mycorhizal fungi
Date: 26 Jan 1995 16:48:21 -0500
Organization: America Online, Inc. (1-800-827-6364)
Lines: 4
Sender: root@newsbf02.news.aol.com
Message-ID: <3g95b5$5a8@newsbf02.news.aol.com>
References: <3g5juu$8rf@bud.peinet.pe.ca>
Reply-To: mycochef@aol.com (MYCOCHEF)
NNTP-Posting-Host: newsbf02.mail.aol.com

hi, contact "Mushroom the Journal of Wild Mushrooming" for mucho info re:
your subject (mycorhizal fungi).  call Maggie Rogers 503.239.4321, the
editor for further stuff.  or contact the Mycological Society of San
Francisco thru Rose Flahery at 408.688.3606


From VPGH51A@prodigy.com Fri Jan 27 20:14:50 EST 1995
Article: 1357 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!swrinde!howland.reston.ans.net!swiss.ans.net!prodigy.com!usenet
From: VPGH51A@prodigy.com (Jim Berlstein)
Newsgroups: bionet.mycology
Subject: Urban Psilocybes
Date: 27 Jan 1995 19:50:47 GMT
Organization: Prodigy Services Company  1-800-PRODIGY
Lines: 11
Distribution: world
Message-ID: <3gbiqn$17i8@usenetw1.news.prodigy.com>
NNTP-Posting-Host: inugap4.news.prodigy.com
X-Newsreader: Version 1.2

Greetings!

I gave a slideshow/talk at the Breitenbush Mushroom Conference in Oregon 
this past October on the "Urban Psilocybes of Portland and Seattle"

I would be eager to give this presentation to any groups who feel like 
flying me around and giving me money.  

Jim




From rshelby@acenet.auburn.edu Fri Jan 27 20:14:53 EST 1995
Article: 1358 of bionet.mycology
Newsgroups: bionet.mycology
Path: bigblue.oit.unc.edu!concert!news-server.ncren.net!news.duke.edu!godot.cc.duq.edu!hudson.lm.com!newsfeed.pitt.edu!gatech!swrinde!emory!darwin.sura.net!news.duc.auburn.edu!aces17!aces20.acenet.auburn.edu!rshelby
From: rshelby@acenet.auburn.edu (Richard A. Shelby)
Subject: Shiitake spawn sales
Message-ID: <rshelby.791044075@aces20.acenet.auburn.edu>
Keywords: Lentinula,Shiitake,Shiang-Gu
Sender: news@acenet.auburn.edu
Nntp-Posting-Host: aces1
Organization: Alabama Cooperative Extension Service, Auburn University
Date: Wed, 25 Jan 1995 14:27:55 GMT
Lines: 12

I am preparing a brief "how-to" manual on backyard shiitake
cultivation for homeowners and would like to include a list of
spawn producers.

If you would like to be included in that listing, please send
particulars including prices, type, strains, etc. to me via
e-mail. 

Thanks,

R. A. Shelby  
Alabama Cooperative Extension Service


From mycochef@aol.com Fri Jan 27 20:14:54 EST 1995
Article: 1359 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!news-server.ncren.net!news.duke.edu!agate!howland.reston.ans.net!swrinde!pipex!uunet!newstf01.news.aol.com!not-for-mail
From: mycochef@aol.com (MYCOCHEF)
Newsgroups: bionet.mycology
Subject: Re: Black Bolete
Date: 25 Jan 1995 10:08:29 -0500
Organization: America Online, Inc. (1-800-827-6364)
Lines: 7
Sender: root@newsbf02.news.aol.com
Message-ID: <3g5phd$q9n@newsbf02.news.aol.com>
References: <3fpla4$pb8@mark.ucdavis.edu>
Reply-To: mycochef@aol.com (MYCOCHEF)

Hi,  see if they are marked "from South America."  If so they may be
boletus luteus sold by Casccade Mushroom Co. in Oregon.  these are very
fragrant, almost chocolatey and cinnamony, and go well with an ersatz
Mexican mole.  This past weekend I cooked for a David Arora foray in Santa
Cruz, CA. and had a large bag of b. luteus to play with ( along with fresh
chanterelles, candy caps, blewits, leccinum manzanitae, and dried edulis).
 rained hard but a lot of fun was had by all. . . .bye


From mycochef@aol.com Fri Jan 27 20:14:56 EST 1995
Article: 1360 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!news-server.ncren.net!news.duke.edu!godot.cc.duq.edu!newsfeed.pitt.edu!gatech!swrinde!pipex!uunet!newstf01.news.aol.com!not-for-mail
From: mycochef@aol.com (MYCOCHEF)
Newsgroups: bionet.mycology
Subject: Re: Portobello
Date: 25 Jan 1995 10:09:08 -0500
Organization: America Online, Inc. (1-800-827-6364)
Lines: 1
Sender: root@newsbf02.news.aol.com
Message-ID: <3g5pik$q9v@newsbf02.news.aol.com>
References: <3fpp4g$pdb@crl12.crl.com>
Reply-To: mycochef@aol.com (MYCOCHEF)

Bob, call me (patrick) and i will reveal this and other secrets. . . .


From mycochef@aol.com Fri Jan 27 20:14:57 EST 1995
Article: 1361 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!news-server.ncren.net!news.duke.edu!godot.cc.duq.edu!hudson.lm.com!newsfeed.pitt.edu!gatech!swrinde!pipex!uunet!newstf01.news.aol.com!not-for-mail
From: mycochef@aol.com (MYCOCHEF)
Newsgroups: bionet.mycology
Subject: Re: Morel Production
Date: 25 Jan 1995 10:11:42 -0500
Organization: America Online, Inc. (1-800-827-6364)
Lines: 2
Sender: root@newsbf02.news.aol.com
Message-ID: <3g5pne$qae@newsbf02.news.aol.com>
References: <9501230808.ZM821@sarg.pgh.wec.com>
Reply-To: mycochef@aol.com (MYCOCHEF)

try Paul Stammets of Fungi Perfecti in Olympia, 
Wash.  call information for the number (area code 206).


From mycochef@aol.com Fri Jan 27 20:14:58 EST 1995
Article: 1362 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!news-server.ncren.net!news.duke.edu!godot.cc.duq.edu!hudson.lm.com!news.pop.psu.edu!psuvax1!uwm.edu!spool.mu.edu!howland.reston.ans.net!swrinde!pipex!uunet!newstf01.news.aol.com!not-for-mail
From: mycochef@aol.com (MYCOCHEF)
Newsgroups: bionet.mycology
Subject: Re: Black Bolete
Date: 25 Jan 1995 10:15:59 -0500
Organization: America Online, Inc. (1-800-827-6364)
Lines: 5
Sender: root@newsbf02.news.aol.com
Message-ID: <3g5pvf$qbi@newsbf02.news.aol.com>
References: <3fv7gl$hue@usenetw1.news.prodigy.com>
Reply-To: mycochef@aol.com (MYCOCHEF)

could be "b. luteus" from South America, a common imported suillus that is
labeled as something else.  in marin county, ca. we are not "waiting for
autumn"--here we are just waiting for the rain to stop!  i did go out
yesterday and gather chanterelles, helvella lacunosa, candy caps, russula
cyanozantha.  lucky us!


From arnold@bscr.uga.edu Sat Jan 28 22:37:15 EST 1995
Article: 1363 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!howland.reston.ans.net!news.moneng.mei.com!uwm.edu!spool.mu.edu!olivea!biosci!GENOME.GENETICS.UGA.EDU!arnold
From: arnold@GENOME.GENETICS.UGA.EDU
Newsgroups: bionet.mycology
Subject: (none)
Date: 27 Jan 1995 10:29:05 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 165
Sender: daemon@net.bio.net
Distribution: world
Message-ID: <0098B172.68C18BC7.66@genome.genetics.uga.edu>
Reply-To: arnold@bscr.uga.edu

		Measuring physical map quality using bootstrap resampling


PROGRAM DESCRIPTION:

        This program is to assist in measuring the reconstructed physical
	map quality using bootstrap resampling.  

	The theory for reconstruction of chromosomes or chromosome fragments
        ("contig mapping") from a clonal library can be found in

        Cuticchia, A.J., Arnold, J., and W.E. Timberlake. (1992a). The
        use of simulated annealing in chromosome reconstruction experiments
        based on binary scoring. Genetics 132: 591-601

	The program that orders DNA sequences is based on similarity of
        their binary profiles assigned to clones in a library by one of
        several experimental approaches. The algorithm has been used
        to map the Schizosaccharomyces pombe genome, the Aspergillus
        nidulans genome, and a region of Human chromosome IX.
        DNA fragments with a high degree of overlap are expected
        to show a high degree of similarity in their profiles.
        The ordering process is based on minimizing the sum of the linking
        distances between clones as a function of their ordering along the
        chromosome. 

	This minimization algorithm used here is a new one called random
	cost. It is detailed in 

	Wang, Y., Prade, R. A., Griffith, J., Timberlake, W.E., and Arnold, J.
	(1994) A fast random cost algorithm for physical mapping. PNAS,
	91, 11094-11098

	In bootstrap resampling, probes are randomly resampled with replacement
	for many times (1000 default). This program calculates how often the 
	links in the original reconstructed map reappear in maps under 
	bootstrap resampling. Three such frequencies are calculated; they are:
        (i) how often two clones appear together- C1; (ii) how often a clone or
        one that is equivalent in hybridization profile appear next to each
        other - C2; (iii) how often two clones are within the same island - C3.
        A description of the bootstrap resampling procedure for assessing
        the reliability of a physical map is described in:

        Wang, Y., Prade, R.A., Griffith, J., Timberlake, W.E., and Arnold, J.
        (1994) ODS_BOOTSTRAP: assessing the statistical reliability
        of a physical map by bootstrap resampling.  CABIOS 10: 625-634

        




PROGRAM INPUT

	A typical batch file is given as follows:

	$set def [wang.cm.bootstrap]
	$run boot
	boot.dat
	probe.dat
	boot.out
	boot.rec
	0

	The first line is to set the default directory.

	The second line is to run this program

	The third line is the input file name. In the input hybridization file,
	the first line should be the number of probes, clones  and bootstrap
        run numbers.  For all other lines, the first ten columns are reserved
        for the clone, name and the hybridization data should start at any
        column after 10th column. Total length of each 
	line is defined by MAX_BUFFER in the program. The example is
        the clone/probe hybridization matrix for Chromosome IV of
        A. nidulans.

	The fourth line is the probe names file name. It contains the probe
        names in the same order across the columns as in the input file.

	The fifth line is the output file name. The finally reconstructed
        physical map, the statistical confidence statistics from the
        bootstrap run and other statistics are written to this file.

	The sixth line is the name of the file that stores the total linking
        distance for each bootstrap run. This is for tracing how the
        program is running.

	The seventh line is the seed for random number generator.

  
PROGRAM INPUT LIMITATIONS:

        The number of clones must be between 1 and 600 for a DEC VAX
	station 4000.

        Filenames (with directory path, if specified) must be
        no longer than 40 characters.

	If the bootstrap run number is not given in the input binary hybridization 
	data file, it will be set to the default number of 1000. 


PROGRAM SPEED:

        The program assembled a physical map of 593  clones
        probed with 115 probes within less than 2 mins on a DEC
	VAX station 4000. Total time for 1000 bootstraps run will take
	30 CPU hours on this workstation.


OBTAINING THE SOFTWARE:

        The software is only distributed via
        Internet using EMAIL. Please send an EMAIL request to:

                    ARNOLD@BSCR.UGA.EDU

        if you wish copies of the program. I will EMAIL you:

        1) a C program  boot.c;

        2) this documentation file, boot.DOC;

        3) a test input file, boot.dat;

	4) a test probe name file, probe.dat

        4) an example output file, boot.dat; and

        5) a command file, boot.COM.

        This last file is what you would use to submit a batch job in
        the VAX/VMS operating system to generate the file boot.out.



SOFTWARE SUPPORT IN THE USE OF THE PROGRAMS:

        If you have questions about
        the programs, please contact Yuhong Wang currently located
        at University of Georgia:

                    wang@bscr.uga.edu
        or myself at
                    arnold@bscr.uga.edu


HARDWARE LIMITATIONS:

        The programs have been run without modification on VAXstations,
        a DECstation 3100,  a Silicon Graphics IRIS 4D70/GT workstation
        and IBM Risc 6000 workstation.



  . - - - - - - - - - - - Jonathan Arnold - - - - - - - - - - - - - - - .
  |                       Dept. of Genetics,                            |
  |                       University of Georgia                         |
  |                       Athens, Georgia 30602                         |
  | Phone:       (706) 542-1449                                         |
  | messages:    (706) 542-8000                                         |
  | FAX:         (706) 542-3910                                         |
  | Internet:    ARNOLD@BSCR.UGA.EDU                                    |
  . - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - .


From kcowing@clark.net Sat Jan 28 22:37:16 EST 1995
Article: 1364 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!howland.reston.ans.net!news.sprintlink.net!news.clark.net!jobrien-ppp.clark.net!user
From: kcowing@clark.net (Keith L. Cowing)
Newsgroups: bionet.plants,bionet.photosynthesis,bionet.mycology,bionet.genome.arabidopsis,bionet.agroforestry,bionet.biology.grasses
Subject: CALL: NASA Research Announcement - Plant Research
Date: Sat, 28 Jan 1995 11:54:39 -0500
Organization: American Institute of Biological Sciences
Lines: 148
Message-ID: <kcowing-2801951154390001@jobrien-ppp.clark.net>
NNTP-Posting-Host: jobrien-ppp.clark.net
Mime-Version: 1.0
Content-Type: TEXT/PLAIN; charset=ISO-8859-1
Content-Transfer-Encoding: 8bit
Xref: bigblue.oit.unc.edu bionet.plants:4931 bionet.photosynthesis:513 bionet.mycology:1364 bionet.genome.arabidopsis:2700 bionet.agroforestry:1096 bionet.biology.grasses:48

The following NASA Research Announcement has been distributed 
by mail (1/26/95) to a standard NASA distribution list.  The 
NRA can be obtained by contacting the NASA officials listed 
below.  At some point soon, you should be able to download 
the entire NRA from either:

       http://www.hq.nasa.gov/

or from the NASA/OLMSA Life and Biomedical Science and 
Applications (LBSAD) BSAD Gopher:

       gopher://lifegopher.hq.nasa.gov/


NOTE: An update for more specific details regarding download 
sites will be posted, if necessary.

++++++++++++++

Excerpt from:

NASA OMB Approval No. 2700-0042
NRA 95 OLMSA-02
NASA Research Announcement Soliciting Proposals for Plant 
Research Utilizing U.S. Space Shuttle Middeck and Russian 
Space Station Mir

Life and Biomedical Sciences and Applications Division
Office of Life and Microgravity Sciences and Applications
National Aeronautics and Space Administration

Date NRA Issued:  January 26, 1995
Letters of Intent Due:    February 24, 1995
Proposals Due:  April 12, 1995

The Life and Biomedical Sciences and Applications Division of 
the National Aeronautics and Space Administration (NASA) 
seeks proposals for research that supports three areas of 
emphasis in the Plant Space Biology Program: 1) the 
understanding of the role of gravity in the growth, 
development, structure, and function of plants; 2) the 
acquisition of scientific information to develop the 
technical base required for the construction and the use of a 
bioregenerative life support system to support crews in 
extraterrestrial environments; and 3) the utilization of 
gravity and microgravity to acquire knowledge for benefits on 
earth.  Space flight investigations conducted to date have 
shown that gravity affects the structure and function of 
plants at all levels (organismal, cellular, subcellular, and 
molecular).  How the processes are controlled, and the role 
gravity plays in them, remain largely unknown.  Although 
plant biology research concentrate upon achieving a 
fundamental scientific understanding of the effects of 
gravity upon plants, it is apparent that the knowledge gained 
will have practical application for environmental management, 
for enhanced production of food, medicine, clothing, and 
forest products on earth, and for the development of life 
support systems for space exploration.  Proposals may 
emphasize molecular, genetic, biochemical, physiological, and 
cellular analyses that aim to explain mechanisms underlying 
the effects of gravity and biochemical controls in plants.  
Other proposals may emphasize more applied research aimed at 
determining plant behavior under microgravity conditions and 
how plants may play a part in bioregenerative systems.  
Proposed research may involve the use of small land plants 
and/or their cells, protoplasts, tissues, organs and seeds.

This NASA Research announcement (NRA) provides an opportunity 
to conduct investigations on plants as a small payload flight 
experiment in he U.S. Space Shuttle middeck, or as a flight 
experiment on the Russian Space Station Mir.  Experiments can 
either be developed as "stand alone" flight experiments 
without ground research (but based upon a firm ground 
research foundation) or in combination with appropriate 
ground research.  Flight opportunities as a small payload 
flown on the U.S. Space Shuttle middeck in the Plant Growth 
Unit (PGU), the Plant Growth Facility (PGF), or in a passive 
container called the Biological Research Canisters (BRIC).  
Plants flown on the Mir will be maintained in the recently 
upgraded Russian growth unit, Svet.  Proposals to this NRA 
are for flight opportunities available from 1995-1997.

Participation in small payloads and Mir research for the 
Space Plant Biology program is open to all individuals and 
all categories of domestic and foreign organizations, 
industry, educational institutions, other nonprofit 
organizations, NASA laboratories, and other U.S. Governmental 
agencies.  Proposals which will enhance or complement the 
scientific return from research currently being supported by 
the National Science Foundation or other Government agencies 
are encouraged.  

A letter of Intent to Propose is requested by February 24, 
1995.  Proposals may be submitted at any time up to April 12, 
1995.  Proposals will be evaluated for scientific/technical 
value, programmatic relevance, implementation feasibility, 
the ability of the experiment to meet hardware and safety 
requirements, and the availability of funds.  Selection for 
flight definition (proposals selected for scientific merit, 
programmatic relevance and feasibility) will be made between 
May and July 1995.  The funding schedule for each selected 
experiment will be dependent on its particular flight 
assignment.

Submit proposal to:

     Dr. Tom Scott
     Life and Biomedical Sciences and Applications Division
     Code ULR
     NASA Headquarters
     Washington, DC 20564-0001

Copies required:  Twenty (20)

NASA Selecting official:

     Dr. Joan Vernikos
     Director, Life and Biomedical Sciences and Applications   
     Division/ Code ULR
     NASA Headquarters
     Washington, DC 20564-0001

Proposals due:  April 12, 1995

Letters of Intent Due:  February 24, 1995

Foreign proposals: details within NRA

Obtain additional information about science objectives from:
     Dr. Tom Scott
     Life and Biomedical Sciences and Applications Division
     Code ULR
     NASA Headquarters
     Washington, DC 20546-0001
     Telephone: (202)-358-2359
     Fax: (202)-358-4168
     E-mail: tscott@gm.olmsa.hq.nasa.gov

Obtain additional information about flight hardware and 
characteristics from:

Ms. Mayra Montrose
     Life and Biomedical Sciences and Applications Division
     Code ULF
     NASA Headquarters
     Washington, DC 20546-0001
     Telephone: (202)-358-2147
     Fax: (202)-358-4168


From mycochef@aol.com Sun Jan 29 17:13:51 EST 1995
Article: 1365 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!swrinde!pipex!uunet!newstf01.news.aol.com!newsbf02.news.aol.com!not-for-mail
From: mycochef@aol.com (MYCOCHEF)
Newsgroups: bionet.mycology
Subject: Re: Urban Psilocybes
Date: 29 Jan 1995 12:12:00 -0500
Organization: America Online, Inc. (1-800-827-6364)
Lines: 5
Sender: root@newsbf02.news.aol.com
Message-ID: <3ggi90$dse@newsbf02.news.aol.com>
References: <3gbiqn$17i8@usenetw1.news.prodigy.com>
Reply-To: mycochef@aol.com (MYCOCHEF)
NNTP-Posting-Host: newsbf02.mail.aol.com

hello, try calling Larry Stickney  at  51O.465.7963.  he is big in the
myco. society of San Francisco.  p.s. i cooked a mushroom meal at the home
of Jim Trappe just after the Breitenbush event.  Eric Danell, David Arora,
and a lot more were there to spread tales and gobble good food.
bye


From bowman@ORCHID.UCSC.EDU Mon Jan 30 20:19:23 EST 1995
Article: 1366 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!howland.reston.ans.net!spool.mu.edu!olivea!biosci!ORCHID.UCSC.EDU!bowman
From: bowman@ORCHID.UCSC.EDU
Newsgroups: bionet.mycology
Subject: Neurospora Workshop
Date: 30 Jan 1995 11:11:36 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 46
Sender: daemon@net.bio.net
Distribution: world
Message-ID: <9501301907.AA08460@biology.UCSC.EDU>
NNTP-Posting-Host: net.bio.net

From: Barry Bowman, Univ. of California, Santa Cruz

As part the Fungal Genetics Conference (21-26 March, 1995, Asilomar CA)
there will be a Neurospora Workshop.  The structure of the workshop will be
similar to that held 2 years ago - two sessions of about 3 hours each.  We
want to keep the sessions as informal as possible,  allow ample time for
questions and discussion, and focus on topics of general interest to those
working with Neurospora.

We have time for approximately 15 presentations, averaging 15 min each.  I
wish to solicit suggestions for speakers.  Is there a particular topic or
person that should be included?  If you would like to make a presentation
yourself, please give me a brief summary of the topic.  I am open to all
suggestions, but unfortunately the time available is limited and I will
probably have to decline many of the requests.  In other words, don't be
afraid to ask,  but don't be upset if I say no.

Some of the topics that might be included are:

Structure of the genome.
	Gene regulation, promoter structure, regulatory elements etc.
	Intron structure, splicing, polyadenylation
	methylation, Ripping, quelling
	transposable elements, endogenous plasmids
	evolution of the genome, relationship to other species
Methods of gene analysis
	gene disruption
	insertional mutagenesis
	libraries and plasmids, YACs etc.
	transformation, electroporation
	regulatable promoters, reporter genes
	Neurospora Genome Project, physical map, sequencing
Cell Biology
	Isolation of organelles and membranes
	Immunofluroescence
	Useful strains, (protease deficient, morphological mutants etc.)

Barry Bowman
Sinsheimer Labs
U. of California
Santa Crux, CA  95064

Tel (408) 459-2245
FAX   "       "     3139
email bowman@orchid.ucsc.edu



From grad@D.UMN.EDU Mon Jan 30 20:19:24 EST 1995
Article: 1367 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!howland.reston.ans.net!spool.mu.edu!olivea!biosci!D.UMN.EDU!grad
From: grad@D.UMN.EDU (stephen hedman)
Newsgroups: bionet.mycology
Subject: Position Announcement
Date: 30 Jan 1995 15:20:01 -0800
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Cell Biologist


The University of Minnesota-Duluth Department of Biology invites
applications for a tenure-track Assistant Professor position beginning
September 1, 1995.  The position requires a Ph.D in a biological science
and postdoctoral research experience.  Responsibilities will include:
teaching cell biology, introductory biology, and advanced specialty
courses; undergraduate advisement; graduate education (M.S. emphasis);
and establishment of a strong extramurally funded research program.
Collaborative research with faculty in one of three University of
Minnesota, Duluth School of Medicine departments (Anatomy and Cell
Biology, Medical Microbiology and Immunology, Medical and Molecular
Physiology) strongly encouraged.  Send curriculum vitae, copies of
undergraduate and graduate transcripts, statement of teaching and
research interests, up to three reprints, and three letters of
recommendation that address teaching and research potential to:  Dr.
Conrad E. Firling, Cell Biology Search Chair, Department of Biology,
University of Minnesota, 10 University Drive, Duluth, Minnesota 55812.
Application deadline, March 20, 1995.  The University of Minnesota is an
Equal Opportunity Educator and Employer.




Contact: Conrad E. Firling, email: cfirling@ub.d.umn.edu; TEL:
218-726-7270; FAX:218-726-8142


From conleyted@delphi.com Tue Jan 31 19:43:16 EST 1995
Article: 1368 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!rutgers!gatech!swrinde!howland.reston.ans.net!news2.near.net!news.delphi.com!usenet
From: Ted Dean Conley <conleyted@delphi.com>
Newsgroups: bionet.mycology
Subject: Where to buy pressure cookers???
Date: Mon, 30 Jan 95 15:00:28 -0500
Organization: Delphi (info@delphi.com email, 800-695-4005 voice)
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I was wondering if anyone knew of a lab supplier (or other
company) who sells pressure cookers at a decent price.  My
purpose is in setting up a sterilization system for growing
mushrooms.  Any advice appreciated.
 
Ted D. Conley.
--
                   --------------------------
                         Ted D. Conley
                      CONLEYTED@delphi.com
                             _   _
                     .oooO  / ) ( \  Oooo.
                     (   ) / (   ) \ (   )
                      \ ( (   ) (   ) ) /
                   ----\_).oooO-Oooo.(_/----
                   70363.1442@compuserve.com
                   -------------------------


From rwinder@PFC.Forestry.CA Tue Jan 31 19:43:18 EST 1995
Article: 1369 of bionet.mycology
Newsgroups: bionet.mycology
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From: rwinder@PFC.Forestry.CA (Richard Winder)
Subject: Re: mycorhizal fungi
Message-ID: <1995Jan30.182138.1415@spruce.pfc.forestry.ca>
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Date: Mon, 30 Jan 1995 18:21:38 GMT
Lines: 98

In article <3g5juu$8rf@bud.peinet.pe.ca>, stewart@bud.peinet.pe.ca 
(Paul Stewart) writes:
>Fellow Myconuts:
>	Has anyone a contact for research or info regarding mycorhizal fungi?
>Specifically, I'm looking for any attempts to culture them.  My idea is 
>to try extracts of the companion roots or surrounding soil as additives 
>to agar and similar additives to the spawn mix (grain, wood chips, sawdust).
>	I have successfully grown mycelium cultures of Boletus edulis 
>(king bolete), chanterelles, and Suillus luteus (slippery Jack), all of 
>which are native to PEI, but not reliably plentiful enough to wild 
>harvest (not crazy about wild harvest anyway).  I've heard from plenty of 
>people that I'm wasting my time, so I'd rather that not be my only reply 
>(Stamets' latest book seems to agree with me).

You are not wasting your time.  Certain boletes (including B. edulis) have 
been reportedly grown in axenic culture.  Ditto for morels.  It will take a bit
of imagination and research to overcome the technical problems in a way that
would be economically feasible, though.

>	Anyone with ideas to share please e-mail me or reply to this 
>group.  I'll continue to be willing to share any of my own findings.  I 
>have already identified some necessary trace elements from soil 
>containing Chanterelle mycelia.  I just took samples from the mycelial 
>layer, the grass root zone, and below this, followed by similar samples a 
>few feet away.  The mycelia seemed to accumulate manganese, boron, and 
>calcium in significant quantities.  Any comments.....?

Just some really crazy ideas, with apologies out there to those who may have
already considered these things...

For culture of mycorrhizal mushrooms in general, I wonder if some of
the issues to contend with are:

1) Intactness/size of mycelium

Let's face it- B. edulis can get pretty big.  Many of these mushrooms probably
need to achieve a certain mycelial size before they start pumping all that
water and nutrients into a fruiting body (hence the use of deep dish agar
cultures to produce Boletus spp.)

2) Water relations

If the medium dries out before a fruiting body can get going, it will abort.
Proper water flow could also be involved in wicking away staling compounds or
inhibitory compounds such as phenolics.

3) Nutrient flux

A clear source/sink flow of nutrients may need to be established.
A disruption of this flux might also be necessary for fruiting.

4) Inhibitors

Some seeds need ample water to leach away inhibitors linked with dormancy- it
wouldn't be shocking to find them in fungi.  At any rate, closed systems
accumulate waste products, inhibitors, etc. much more readily than open ones.
Hence the incorporation of orange slices in successful Armillaria spp. 
mushroom culture (I think that the ascorbic acid/Vitamin C/antioxidant helps 
the fungus deal with accumulated phenolic compounds and other inhibitors- 
but watch that pH!).

5) Templates

The orange peels described above also provide a good hunk of cellulose which
probably acts as a nutritious growth template.

6) Photoperiod/hormones

The B. edulis in my front yard (no, you can't have my home address!) always
fruit exactly on Canada day (July 1) with the Amanita muscaria 2 days behind.
The involvement of photoperiod might be direct, or indirect (plant hormones,
etc.).

7) Nutrient base

Approximating more closely the amino acids and sugars present in a tree root 
which are being exported to the mycelium might be a big help.  Pectins,
suberin, and various cell wall constituents  might help.  Maybe you also
need phosphorylated compounds, membrane compounds (glycolipids, DNA fragments,
etc.), or triggers like c-AMP to indicate a "living" presence- who knows. 

8) Microbes

Chantrelles and truffles apparently have a good deal of bacteria incorporated
into their sporocarps, which may indicate symbiosis with other microbes as well
as plants.  It might be that bacterial cell wall components could be
incorporated into the medium to avoid the contamination problems posed
by these symbionts, which are usually facultative saprobes.  If the bacteria 
produce iron-abosorbing ionophores which the fungus can use, this might be
another thing to look into.  Various microbes (bacteria, yeasts) may not be
true symbionts, but may aid in sporocarp formation by providing competitve
stress or an environmental cue.

Still ready to grow boletes commercially?	-RSW

  RICHARD WINDER                    Title: Research Scientist
  Canadian Forest Service           Phone: (604) 363-0773
  Victoria, B.C.                    Internet: RWINDER@A1.PFC.Forestry.CA


From msachs@ADMIN.OGI.EDU Tue Jan 31 19:43:19 EST 1995
Article: 1370 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!bloom-beacon.mit.edu!spool.mu.edu!uwm.edu!biosci!ADMIN.OGI.EDU!msachs
From: msachs@ADMIN.OGI.EDU (Matthew Sachs)
Newsgroups: bionet.mycology
Subject: Re: ropy mutants of N. crassa?
Date: 31 Jan 1995 09:48:50 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
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>At a meeting last year, someone mentioned ropy (?) mutants
>of N. crassa in connection with hyphal branching, nuclear movement,
>morphology etc.
>It was said there were 11 genes, and 3 had been cloned.
>I tried to find reference to these in recent literature,
>but could not.  Can anyone give me a reference to
>these mutants (maybe I misheard the name!).
>
>
>--
>Geoff Turner
> University of Sheffield

Michael Plamann's group has been doing fascinating work with ropy mutants.

Plamann, M., Minke, P. E., Tinsley, J. H., and Bruno, K. S. (1994).
Cytoplasmic dynein and actin-related protein Arp1 are required for normal
nuclear distribution in filamentous fungi. J. Cell Biol. 127, 139-149.



-----------------------------------------------------------
Matthew Sachs
Department of Chemistry, Biochemistry and Molecular Biology
Oregon Graduate Institute of Science and Technology
20000 NW Walker Road
P.O. Box 91000
Portland, OR  97291-1000
503 690-1487 Phone
503 690-1464 Fax
msachs@admin.ogi.edu




From mzieg1234@aol.com Wed Feb  1 12:56:53 EST 1995
Article: 1371 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!swrinde!pipex!uunet!newstf01.news.aol.com!newsbf02.news.aol.com!not-for-mail
From: mzieg1234@aol.com (MZieg1234)
Newsgroups: bionet.mycology
Subject: Re: Where to buy pressure cookers???
Date: 31 Jan 1995 21:07:30 -0500
Organization: America Online, Inc. (1-800-827-6364)
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NNTP-Posting-Host: newsbf02.mail.aol.com

Gardener's Supply in Burlington VT sells the 941 canner for around $200- a
very good price. Also check your state and US surplus sometimes they have
very good deals on large (or small) autoclaves.


From lmelvill@uoguelph.ca Wed Feb  1 23:42:26 EST 1995
Article: 1372 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!news-server.ncren.net!news.duke.edu!solaris.cc.vt.edu!news.alpha.net!uwm.edu!news.moneng.mei.com!howland.reston.ans.net!torn!ccshst05.cs.uoguelph.ca!ccshst01.cs.uoguelph.ca!lmelvill
From: lmelvill@uoguelph.ca (Lewis Melville)
Newsgroups: bionet.mycology
Subject: Re: Mycorrhizal fungi mailing list
Date: 31 Jan 1995 16:14:02 GMT
Organization: University of Guelph
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SEIFERTK@NCCCOT.AGR.CA wrote:

	Please add your name after the subscribe micronet message.

: To join: Send the following message to listserv@uoguelph.ca 

	subscribe micronet <your name>

: For information:  contact Dr. Lewis Melville at lmelvill@uoguelph.ca 

Thanks...lewis melville


From stewart@bud.peinet.pe.ca Wed Feb  1 23:42:28 EST 1995
Article: 1373 of bionet.mycology
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From: stewart@bud.peinet.pe.ca (Paul Stewart)
Newsgroups: bionet.mycology
Subject: Re: mycorhizal fungi
Date: 31 Jan 1995 12:25:48 -0400
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NNTP-Posting-Host: bud.peinet.pe.ca

My sincere thanks to all who have responded so far to my inquiries into 
mycorrhizal culture:
Ralph Arnold		Wayne Harrison		Mycochef
"jagput"		Keith Seifert		Tom Volk
Richard Winder		and any others I may have lost track of.

Nothing so far has dampened my motivation. Since I am only at the stage 
of growing mycelia of morel, boletes, and chanterelle, I have ample 
opportunity to become discouraged in the coming months :-) .

I will be moving the mycelia to some sort of spawn mixture in the coming 
weeks, and all your suggestions will be tried, along with any other wacky 
suggestions y'all come up with. Glad to see there is so much interest in 
this topic. It certainly isn't a big thing in our University library, 
which is mostly funded these days by the Vet school (anyone know of a 
smily for slightly bitter resentment?)

For what it's worth, I would be very interested in trading mycelia with 
others in Canada.  Does anyone have experience with the regulations and 
methods of shipping cultures outside Canada or the US?

For what it's further worth, I will gladly share what small successes I 
may trip over in the coming months. Thanks again.....PAUL


From stamets1@aol.com Wed Feb  1 23:42:30 EST 1995
Article: 1374 of bionet.mycology
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From: stamets1@aol.com (Stamets 1)
Newsgroups: bionet.mycology
Subject: Re: Where to buy pressure cookers???
Date: 31 Jan 1995 10:58:25 -0500
Organization: America Online, Inc. (1-800-827-6364)
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Ted et alia- 

Our company, Fungi Perfecti, has the best prices in the country. If you
provide us with a mailing address, we will send you a complete catalogue
free of charge. (Our catalogues are free of charge to all Interneters.) 
We carry a large inventory and ship within 24 hours of payment.
Furthermore,
we accept university purchase orders.Our laboratory product line is
customized
for the needs of fungal tissue culture. We have a rapidly developing
product line
and have several new catalogues in production. 

Fungi Perfecti 800-780-9126///fungi-fax 360-426-9377


From SEIFERTK@NCCCOT.AGR.CA Wed Feb  1 23:42:31 EST 1995
Article: 1375 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!swrinde!sgiblab!spool.mu.edu!olivea!biosci!NCCCOT.AGR.CA!SEIFERTK
From: SEIFERTK@NCCCOT.AGR.CA
Newsgroups: bionet.mycology
Subject: Biological delignification of pulp
Date: 1 Feb 1995 11:05:21 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
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Message-ID: <01HMJD8K44F6003AS1@GW.AGR.CA>

The recent post on the use of white rot fungi to delignify
pulp lead me to contact a 'ringer' to get a good answer...

    Delignification of pulps with enzymes is an active research area,
lying somewhere between a scientific curiosity and a practical
process.  The use of xylanase to aid delignification and decrease (by
ca. 25%) the amounts of chemicals required for bleaching is well
established in Scandinavian and Canadian mills.  Several large enzyme
suppliers produce xylanase for the pulp bleaching market, often from
Trichoderma, and competition to improve enzyme properties and lower
price is intense.  The use of oxidative enzymes from white-rot fungi 
that can directly attack lignin is a second generation approach,
which could produce larger chemical savings than xylanase, but has not
yet been developed to the mill scale.  It is being studied at the
Pulp and Paper Research Institute of Canada, several laboratories in
Japan, and at Lignozym, a private research company in Germany.
Enzyme and chemical suppliers are keenly interested, and there is
likely more research going on but not yet publicized.  Certain white-
rot fungi can delignify kraft pulps, increasing their brightness and
their responsiveness to brightening with chemicals.  The fungal
treatments are too slow, but the enzymes manganese peroxidase and
laccase can also delignify pulps, and enzymatic processes are likely
to be easier to optimize and apply than the fungal treatments.
Progress up to 1993 was reviewed by Reid and Paice (FEMS Microbiology
Reviews 13 (1994) 369-376).  Pulp delignification with laccase is
enhanced by the presence of a compound named ABTS, by an unknown
mechanism.  In the spring of 1994, Hans-Peter Call of Lignozym
announced the discovery of a superior, but unnamed, "mediator" that
also enhanced the action of laccase on pulp, allowing 66% lignin
removal.  For the rest of 1994, the grapevine buzzed with
speculations about the identity of Call's mediator.  His patent
application has just been published (WO 94/29510), and we now know
that the mediator is hydroxybenztriazole (HBT).  Call has reported
successful pilot plant trials, but there are concerns that HBT is too
expensive for an economical process.  Development work on laccase-
mediator systems and on manganese peroxidase continues; further
advances will probably be reported at the International Conference on
Biotechnology in the Pulp and Paper Industry in Vienna this June.
 
Ian D. Reid,
Pulp and Paper Research Institute of Canada (PAPRICAN)
REID@paprican.ca



From mb1gt@sunc.sheffield.ac.uk Wed Feb  1 23:42:32 EST 1995
Article: 1376 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!news-server.ncren.net!news.duke.edu!agate!howland.reston.ans.net!pipex!lyra.csx.cam.ac.uk!warwick!news.shef.ac.uk!sunc!mb1gt
From: mb1gt@sunc.sheffield.ac.uk (G Turner)
Newsgroups: bionet.mycology
Subject: ropy mutants of N. crassa?
Date: 31 Jan 1995 16:47:10 GMT
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At a meeting last year, someone mentioned ropy (?) mutants 
of N. crassa in connection with hyphal branching, nuclear movement, 
morphology etc.
It was said there were 11 genes, and 3 had been cloned.
I tried to find reference to these in recent literature,
but could not.  Can anyone give me a reference to 
these mutants (maybe I misheard the name!).


--
Geoff Turner
 University of Sheffield


From conleyted@delphi.com Thu Feb  2 10:46:46 EST 1995
Article: 1377 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!hearst.acc.Virginia.EDU!portal.gmu.edu!europa.eng.gtefsd.com!howland.reston.ans.net!news2.near.net!news.delphi.com!usenet
From: Ted Dean Conley <conleyted@delphi.com>
Newsgroups: bionet.mycology
Subject: Re: Where to buy pressure cookers???
Date: Wed, 1 Feb 95 15:13:29 -0500
Organization: Delphi (info@delphi.com email, 800-695-4005 voice)
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NNTP-Posting-Host: bos1a.delphi.com
X-To: MZieg1234 <mzieg1234@aol.com>

MZieg1234 <mzieg1234@aol.com> writes:
 
>Gardener's Supply in Burlington VT sells the 941 canner for around $200- a
>very good price. Also check your state and US surplus sometimes they have
>very good deals on large (or small) autoclaves.
 
Thanks for the TIP!
 
Ted.
--
                   --------------------------
                         Ted D. Conley
                      CONLEYTED@delphi.com
                             _   _
                     .oooO  / ) ( \  Oooo.
                     (   ) / (   ) \ (   )
                      \ ( (   ) (   ) ) /
                   ----\_).oooO-Oooo.(_/----
                   70363.1442@compuserve.com
                   -------------------------


From hfromageot@aol.com Thu Feb  2 19:05:40 EST 1995
Article: 1378 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!rutgers!gatech!howland.reston.ans.net!pipex!uunet!newstf01.news.aol.com!newsbf02.news.aol.com!not-for-mail
From: hfromageot@aol.com (HFromageot)
Newsgroups: bionet.mycology
Subject: Re: Wood Pulp Delignification Using Fungi
Date: 1 Feb 1995 18:50:32 -0500
Organization: America Online, Inc. (1-800-827-6364)
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NNTP-Posting-Host: newsbf02.mail.aol.com

Yes, I heard of various mediators involved in laccase-oxygen oxidation of
lignin among other substrates.  The German inventor, Dr. H.-P. Call,
mentioned recently that such mediators belonged to any of the following
four classes of organic compounds: N-oxides, N-hydroxides, oximes and
hydroxamic acids.  I am really interested to know whether anyone else
found similar results.  Please keep me posted.


From lmelvill@uoguelph.ca Thu Feb  2 19:05:43 EST 1995
Article: 1379 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!howland.reston.ans.net!torn!ccshst05.cs.uoguelph.ca!ccshst01.cs.uoguelph.ca!lmelvill
From: lmelvill@uoguelph.ca (Lewis Melville)
Newsgroups: bionet.mycology
Subject: Re: mycorhizal fungi
Date: 2 Feb 1995 21:20:07 GMT
Organization: University of Guelph
Lines: 21
Distribution: world
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NNTP-Posting-Host: ccshst01.cs.uoguelph.ca
X-Newsreader: TIN [version 1.2 PL2]

Paul Stewart (stewart@bud.peinet.pe.ca) wrote:
: Fellow Myconuts:
: 	Has anyone a contact for research or info regarding mycorhizal fungi?
: Specifically, I'm looking for any attempts to culture them.  My idea is 
: to try extracts of the companion roots or surrounding soil as additives 
: to agar and similar additives to the spawn mix (grain, wood chips, sawdust).

	We published a book called Practical Methods in Mycorrhiza 
Research which contains information on culturing and isolating Ecto 
fungi. It is available from Mycologue PUblications, 8728 Lochside Drive, 
Sidney, B.C. Canada. V8L 1M8. U.S. $23.00. 

	Culturing VAM fungi is a different story, usually done in pot 
cultures with a host plant. 
 
	You could also join the micronet listserv list, and contact 
mycorrhiza reseachers that way. SEnd a mail message to 
listserv@uoguelph.ca with the message 
subscribe micronet <your name> 
Good luck. lewis melville.



From spencal@NEXTLAB.CALSTATELA.EDU Fri Feb  3 08:30:00 EST 1995
Article: 1380 of bionet.mycology
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From: spencal@NEXTLAB.CALSTATELA.EDU (Steve Pencall)
Newsgroups: bionet.mycology
Subject: LA MUSHROOM FAIR
Date: 1 Feb 1995 17:27:41 -0800
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NNTP-Posting-Host: net.bio.net


Wild Mushroom Fair, February 19, 1995

With this year's heavy rains, a bumper crop of mushrooms is assured in nearby fields and forests.  To celebrate this  
bounty, the Los Angeles Mycological Society will be staging a Wild Mushroom Fair at the Los Angeles State and County  
Arboretum, 301 North Baldwin Avenue, Arcadia, California on Sunday, February 19, 1995 from 9:00 a.m. to 4:30 p.m.   
Admission is free with admission to the Arboretum ($3.00).

The Fair will feature identified species of Wild Mushrooms displayed in natural habitat settings.  Mushroom experts will be  
on hand to answer all your questions about Wild Mushrooms and you are invited to bring in Wild Mushrooms for  
identification.  A variety of Mushroom books and posters will be offered for sale.  The featured speaker will be Dr. Chester  
R. Leathers, retired professor of botany at Arizona State University in Tempe.  Dr.  Leathers will deliver an illustrated talk  
on "An Introduction to Mushrooms and Their Identification".

On Saturday, February 18, there will be 3 Mushroom walks, or forays, to collect Wild Mushrooms for display at the Fair.   
One foray will be at Malibu Creek State Park, one at Placerita Canyon County Park and one in the Cleveland National  
Forest east of Orange County.  The public is invited to participate in these forays free of charge.

Please call the Los Angeles Mycological Society at (213) 292-1900 for up to date information on the Mushroom forays  
and Wild Mushroom Fair.



From spencal@NEXTLAB.CALSTATELA.EDU Fri Feb  3 08:30:02 EST 1995
Article: 1381 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!rutgers!gatech!swrinde!howland.reston.ans.net!spool.mu.edu!olivea!biosci!NEXTLAB.CALSTATELA.EDU!spencal
From: spencal@NEXTLAB.CALSTATELA.EDU (Steve Pencall)
Newsgroups: bionet.mycology
Subject: LA MUSHROOM FAIR
Date: 1 Feb 1995 17:30:55 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 61
Sender: daemon@net.bio.net
Distribution: world
Message-ID: <9502020122.AA15693@nextlab.calstatela.edu>
NNTP-Posting-Host: net.bio.net


Wild Mushroom Fair, February 19, 1995

With this year's heavy rains, a bumper crop of mushrooms is assured  
in nearby fields and forests.  To celebrate this bounty, the Los  
Angeles Mycological Society will be staging a Wild Mushroom Fair at  
the Los Angeles State and County Arboretum, 301 North Baldwin Avenue,  
Arcadia, California on Sunday, February 19, 1995 from 9:00 a.m. to  
4:30 p.m.  Admission is free with admission to the Arboretum  
($3.00).

The Fair will feature identified species of Wild Mushrooms displayed  
in natural habitat settings.  Mushroom experts will be on hand to  
answer all your questions about Wild Mushrooms and you are invited to  
bring in Wild Mushrooms for identification.  A variety of Mushroom  
books and posters will be offered for sale.  The featured speaker  
will be Dr. Chester R. Leathers, retired professor of botany at  
Arizona State University in Tempe.  Dr.  Leathers will deliver an  
illustrated talk on "An Introduction to Mushrooms and Their  
Identification".

On Saturday, February 18, there will be 3 Mushroom walks, or forays,  
to collect Wild Mushrooms for display at the Fair.  One foray will be  
at Malibu Creek State Park, one at Placerita Canyon County Park and  
one in the Cleveland National Forest east of Orange County.  The  
public is invited to participate in these forays free of charge.

Please call the Los Angeles Mycological Society at (213) 292-1900 for  
up to date information on the Mushroom forays and Wild Mushroom  
Fair.

Wild Mushroom Fair, February 19, 1995

With this year's heavy rains, a bumper crop of mushrooms is assured  
in nearby fields and forests.  To celebrate this bounty, the Los  
Angeles Mycological Society will be staging a Wild Mushroom Fair at  
the Los Angeles State and County Arboretum, 301 North Baldwin Avenue,  
Arcadia, California on Sunday, February 19, 1995 from 9:00 a.m. to  
4:30 p.m.  Admission is free with admission to the Arboretum  
($3.00).

The Fair will feature identified species of Wild Mushrooms displayed  
in natural habitat settings.  Mushroom experts will be on hand to  
answer all your questions about Wild Mushrooms and you are invited to  
bring in Wild Mushrooms for identification.  A variety of Mushroom  
books and posters will be offered for sale.  The featured speaker  
will be Dr. Chester R. Leathers, retired professor of botany at  
Arizona State University in Tempe.  Dr.  Leathers will deliver an  
illustrated talk on "An Introduction to Mushrooms and Their  
Identification".

On Saturday, February 18, there will be 3 Mushroom walks, or forays,  
to collect Wild Mushrooms for display at the Fair.  One foray will be  
at Malibu Creek State Park, one at Placerita Canyon County Park and  
one in the Cleveland National Forest east of Orange County.  The  
public is invited to participate in these forays free of charge.

Please call the Los Angeles Mycological Society at (213) 292-1900 for  
up to date information on the Mushroom forays and Wild Mushroom  
Fair.



From bardonia@aol.com Fri Feb  3 08:30:03 EST 1995
Article: 1382 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!news-server.ncren.net!news.duke.edu!godot.cc.duq.edu!newsfeed.pitt.edu!gatech!howland.reston.ans.net!news.sprintlink.net!cs.utexas.edu!uunet!newstf01.news.aol.com!newsbf02.news.aol.com!not-for-mail
From: bardonia@aol.com (Bardonia)
Newsgroups: bionet.mycology
Subject: Wood Pulp Delignification Using Fungi
Date: 31 Jan 1995 19:30:06 -0500
Organization: America Online, Inc. (1-800-827-6364)
Lines: 5
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There appears to be a lot of research on the use of enzymes from fungi to
delignify wood pulp though the processes do not appear to be practical.
Recent reports from Germany indicate that laccase can delignify pulp in
the presence of certain mediators. What is known about this process? Is
this a practical process or just a scientific curiosity?


From jdkirkla@prairienet.org Fri Feb  3 08:30:04 EST 1995
Article: 1383 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!howland.reston.ans.net!vixen.cso.uiuc.edu!prairienet.org!jdkirkla
From: jdkirkla@prairienet.org (Justin D. Kirkland)
Newsgroups: bionet.mycology
Subject: I need Pleurotus slides
Date: 3 Feb 1995 04:42:41 GMT
Organization: University of Illinois at Urbana
Lines: 14
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NNTP-Posting-Host: firefly.prairienet.org


I am a senior at the University of Illinois and an agronomy 
major. I am enrolled in a required senior seminar class where
we basically give presentations to our peers.  The idea is to
loosen us up for interviews or future job presentations.
	My main area of interest is mushroom production and
would like to give a presentation on Oyster mushroom production.
I am familar with the subject---Stamet's basic course, his books,
etc...  However living in central IL there are no slides
available.  I would like to borrow, rent, purchase, or trade
for slides.  I titled my presentation "From Spore to Store"
and would appreciate any response.  My presentation is Feb 23.
Thanks very much--Justin Kirkland
-- 


From 0002745871@mcimail.com Fri Feb  3 21:35:13 EST 1995
Article: 1384 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!news-server.ncren.net!news.duke.edu!godot.cc.duq.edu!hudson.lm.com!news.pop.psu.edu!news.cac.psu.edu!howland.reston.ans.net!spool.mu.edu!olivea!biosci!mcimail.com!0002745871
From: 0002745871@mcimail.com ("Albert K. Bates")
Newsgroups: bionet.mycology
Subject: Oooops!
Date: 31 Jan 1995 18:55:19 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 8
Sender: daemon@net.bio.net
Distribution: world
Message-ID: <95950201004359.0002745871NA2EM@MCIMAIL.COM>

The diagram appearing in announcements for the Mushroompeople workshop for  
March 11, 1995 with Bob Harris is from the book, The Mushroom Cultivator by 
Paul  Stamets and Jeff Chilton, Copyright ) 1983 Agarikon Press and Growing 
Gourmet & Medicinal Mushrooms by Paul Stamets, Copyright ) 1993 Paul Stamets. 
Neither  Mr. Stamets nor Mr. Chilton is affiliated or connected with 
Mushroompeople, Bob  Harris, or Mr. Harris' workshop. The diagram was used 
without permission, and  Mushroompeople apologizes for its use.  



From VPGH51A@prodigy.com Fri Feb  3 21:35:15 EST 1995
Article: 1385 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!news-server.ncren.net!news.duke.edu!godot.cc.duq.edu!hudson.lm.com!news.pop.psu.edu!news.cac.psu.edu!howland.reston.ans.net!swiss.ans.net!prodigy.com!usenet
From: VPGH51A@prodigy.com (Jim Berlstein)
Newsgroups: bionet.mycology
Subject: Re: Wood Pulp Delignification Using Fungi
Date: 1 Feb 1995 04:29:44 GMT
Organization: Prodigy Services Company  1-800-PRODIGY
Lines: 9
Distribution: world
Message-ID: <3gn2no$e5a@usenetw1.news.prodigy.com>
NNTP-Posting-Host: inugap4.news.prodigy.com
X-Newsreader: Version 1.2

Dr. Mike Gold at the Oregon Graduate Institute in Beaverton, Ore.
was doing something in this area (years ago, before I decided that 
biochemistry was a slow and boring way to die).

However, I believe he knows quite a bit about this.

If you contact him you might NOT want to mention my name, as I 
disappeared without a trace from that facility, thank god.



From tuerk@edvz.sbg.ac.at Fri Feb  3 21:35:16 EST 1995
Article: 1386 of bionet.mycology
Newsgroups: bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!swrinde!cs.utexas.edu!uunet!newsfeed.ACO.net!edvz.sbg.ac.at!DPFL03!tuerk
From: tuerk@edvz.sbg.ac.at (Roman Tuerk)
Subject: Third IAL Symposium- IAL3
Message-ID: <tuerk.2.2F321608@edvz.sbg.ac.at>
Keywords: Lichen, International Association fro Lichenology, WWW
Lines: 90
Sender: news@wst.edvz.sbg.ac.at (USENET News System)
Organization: Institut of Plant Physiology - University of Salzburg
X-Newsreader: Trumpet for Windows [Version 1.0 Rev A]
Date: Fri, 3 Feb 1995 11:39:52 GMT

Third IAL Symposium: preliminary announcement

(online WWW - Informations:  http://www.edvz.sbg.ac.at/pfl/ial3/prelimi.htm)

Third IAL Symposium - IAL3: preliminary announcement
Progress and Problems in Lichenology in the Nineties

(Salzburg, Austria 1 - 7 September 1996)

Preliminary announcement

Arranged by the Intitute of Plant Physiology, University of Salzburg and the 
International Association for Lichenology

Organisation
I. Kärnefelt, President
R. Türk, Secretary

Local organizing Committee and Advisory Board
O. Breuss, G. Gärtner, J. Hafellner, H. Mayrhofer, E. Stocker-Wörgötter, J. Üblagger

General Information
The third international meeting arranged by the IAL and the Insitute of Plant 
Physiology of the University of Salzburg will be held from 1st to 7th September 1996
at the University of Salzburg, Faculty of Natural Sciences.

This symposium continues the series of international meetings on lichen biology which
started at Berlin 1969. It is open to any person interested in lichenized fungi or 
working in fields such as morphology, physiology, ecology, chemistry and systematics
of lichens.

Preliminary Announcement and Second Circular
If you are interested in receiving further information on the symposium please contact
the scientific secretary. The second circular will be distributed during May 1995.

Language
The language at the Congress will be English.

Scientific Programme
The symposium will include oral contributions and poster sessions. It is planed to 
avoid simultaneous sessions. The preliminary scientific programme includes nine 
different sections:

A. Systematics
B. Morphology
C. Ecology
D. Chemistry
E. Changes of Environment and Conservation
F. Resynthesis and Coltivation
G. Lichens of High Mountains
H. Photobionts
I. Lichens in the Tropics

Scientific Sessions
An estimated 20 minutes, comprising a 15 minutes lecture and a 5 minutes discussion
will be allowed for each lecture.

Contributed Papers
Contributed papers may be accepted either as lectures or as posters depending on the
decision of the convenors.

Travel and Accomodations
Salzburg can be reached by train and by air. There is the possibility to arrive by
air in Vienna (Austria) or in Munich (Germany) and take the regional train to Salzburg.
All the symposium partecipants can be offered hotel accomodation in modern student
dormitories wich are used as hotel during summer time for a reasonable price.
Camping accomodation is also available

Congress Fee
The Congress Fee will be estimated at 2.200.-- ATS (approximately equal to 220.-- $)
for full members and 1.100.-- ATS for students.

Excursions
Four different excursions are offered before and after the Congress. Details will be
presented in the second circular.

Correspondence
All correspondence and inquiries should be adressed to the scientific secretariat:

Dr. Roman Türk
University of Salzburg, Institute of Plant Physiology
Hellbrunnerstr. 34
A - 5020 Salzburg, Austria

Telephone Nat.: 0662 8044 5588; 0662 8044 5559
Telephone Int.: +43 662 8044 5588;  +43 662 8044 5559
Fax: +43 662 8044 5010
E-mail: tuerk@edvz.sbg.ac.at




From jfrigot@ucdavis.edu Fri Feb  3 21:35:17 EST 1995
Article: 1387 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!howland.reston.ans.net!agate!news.ucdavis.edu!jerome.ucdavis.edu!user
From: jfrigot@ucdavis.edu (Jerome Rigot)
Newsgroups: bionet.mycology
Subject: fungal-mediated bioremediation research funding
Date: Fri, 03 Feb 1995 08:37:57 -0800
Organization: UCDavis
Lines: 17
Message-ID: <jfrigot-0302950837570001@jerome.ucdavis.edu>
NNTP-Posting-Host: jerome.ucdavis.edu

Hello, 
   I am a grad student doing research at UCDavis in soil bioremediation,
using soil fungi (not white rot).  Unfortunatly, our funding through the
EPA Superfund program got cut.  I would like to finish my work, and I was
wondering if any of you would know of funding sources to which I could
apply to request some support for next year (95-96).  Any resources would
be appreciated concerning private or public funding.  E-write to me
directly, or post on the newsgroup, whichever you prefer.  Thank you,
Jerome.

-- 
Jerome Rigot
Dept. Environmental Toxicology
University of California
Davis, CA 95616
(916)752-5896
e-mail: jfrigot@ucdavis.edu


From rwinder@PFC.Forestry.CA Fri Feb  3 21:35:18 EST 1995
Article: 1388 of bionet.mycology
Newsgroups: bionet.mycology
Path: bigblue.oit.unc.edu!concert!rutgers!gatech!newsxfer.itd.umich.edu!nntp.cs.ubc.ca!news.UVic.CA!spruce.pfc.forestry.ca!news.pfc.forestry.ca!PFC.Forestry.CA!RWINDER
From: rwinder@PFC.Forestry.CA (Richard Winder)
Subject: Growing mushrooms on Internet
X-Nntp-Posting-Host: pfc.pfc.forestry.ca
Message-ID: <D3C95C.L2y@news.pfc.forestry.ca>
Sender: news@news.pfc.forestry.ca (0000-news(0000))
Reply-To: rwinder@PFC.Forestry.CA
Organization: Forestry Canada (Pacific Forestry Centre)
Date: Wed, 1 Feb 1995 20:34:23 GMT
Lines: 88

Dear mushroom fans,

For those who have been following the mushroom cultivation thread, I just 
spied the following in rec.gardens.

Subject: MUSHROOM GROWING (hobby & comm'l)
Message-ID: <57380.rarnold@teleport.com>
From: "Ralph D. Arnold" <rarnold@teleport.com>
Date: Sat, 28 Jan 95 12:25:19 PST
Reply-To: <rarnold@teleport.com>
Organization: Teleport - Portland's Public Access (503) 220-1016

Do you enjoy eating mushrooms, especially gourmet/wild ones?  Would you
like to learn how to grow them for fun, for profit(!), or ecology 
(recycling)??  Perhaps this will interest you?

You can now subscribe to FUNGUS, a free Internet mailing list
dedicated to mushroom cultivation (both hobby and commercial) and related
mycology resources.

I. Philosophical Statement about FUNGUS
FUNGUS has been designed as a vehicle for mushroom growers (both hobbyists
and professionals) and interested parties can have one more Internet access
point, for dissemination of information and for discussions relating to
the cultivation of edible and other fungi.  Conceiveable, there will be
a variety of commercial entities publicized and involved.  This is meant to
be a practical approach to cultivation.

II. Important Non-Internet Resources for the Mushroom Grower
This FREE Internet tool is IN NO WAY meant to replace/supplant other
resources.  I suggest all growers utilize this partial list of resources
<please let me know of add'l resources I should include)

BOOKS:
There are many fine mushroom cultivation books.  In the opinion of the 
moderator, all growers should own "Growing Gourmet & Medicinal Mushrooms"
by Paul Stamets (c) 1993 Ten Speed Press ISBN 0-89815-608-4

MAGAZINES\NEWSLETTERS (Please tell 'em you saw it here)

Cultivated Mushroom Report, U of Toronto, Mississauga, Ontario, Canada

the Mushroom Growers' Newsletter, mycoworld@aol.com, 464 Fulton St,
   Klamath Falls, OR   97601

Mushroom - the Journal, PO Box 3156, Moscow, ID  83843

Mushroom News, c/o American Mushroom Institute, 907 East Baltimore Pike,
   Kennett Square, PA   19348

Shiitake News, Forest Resource Center, Rt2 Box 156A, Lanesboro, MN  55949

ORGANIZATIONS/ASSOCIATIONS/SEMINARS
These are too numerous to include now.  Hopefully, each portion of every 
catagory will be "advertised" or announced in messages to FUNGUS


III. Other Internet Sites Where Archived Messages Are Located

I have setup a GOPHERSPACE where much of this info will be stored.
Access it :

VIA WWW at gopher://gopher.teleport.com/11/users/rarnold

VIA Gopher  gopher.teleport.com  :select "users"; then "on-line access";
                         "fungi-by-email"

VIA Vernonica    search "Fungi-By-Email"

I have also set up an anonymous FTP site containing these files. In 
addition, ARCHIVED messages/textfiles will be stored in this area.

VIA FTP at ftp.teleport.com in directory \users\rarnold\mushroom
/////////
IV.  How to Subscribe to FUNGUS
Send an email message to fungus-request@teleport.com with this message:
SUBSCRIBE FUNGUS <your individual email address>
  B. How to Unsubscribe to FUNGUS
Send an email message to fungus-request@teleport.com with this message:
UNSUBSCRIBE FUNGUS <your email address>
..

V.  Any questions/comments can be directed to the moderator, which is
Ralph D. Arnold.  He can be reached at rarnold@teleport.com.

  RICHARD WINDER                    Title: Research Scientist
  Canadian Forest Service           Phone: (604) 363-0773
  Victoria, B.C.                    Internet: RWINDER@A1.PFC.Forestry.CA


From umbjc@GEMINI.OSCS.MONTANA.EDU Sat Feb  4 12:12:59 EST 1995
Article: 1389 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!taco.cc.ncsu.edu!biosci!GEMINI.OSCS.MONTANA.EDU!umbjc
From: umbjc@GEMINI.OSCS.MONTANA.EDU (Jimmy Cutler)
Newsgroups: bionet.mycology
Subject: Medical Mycology Predoc Train.
Date: 3 Feb 1995 10:56:32 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 68
Sender: daemon@net.bio.net
Distribution: world
Message-ID: <Pine.3.87.9502031142.A18371-0100000@gemini.oscs.montana.edu>

Announcement: Establishment of a Medical Mycology Predoctoral Training 
Program (MMPTP) at Montana State University
	The number of fungal infections has increased dramatically over 
the past decade and is expected to continue to increase.  People who have 
immunological deficiencies are especially at risk.  The deficiencies may 
occur because of infectious diseases such as AIDS, or because of modern 
medical procedures such as kidney and bone marrow transplants, indwelling 
catheters, the use of corticosteroids, broad spectrum antibiotics and 
anti-tumor agents.  The NIH recognizes the need for scientists trained to 
do research in medical mycology.  To help address this need, we have been 
awarded a training grant from the National Institute of Allergy and 
Infectious Diseases to establish the MMPTP.

MMPTP Faculty and Research Facilities:
	The intent of the MMPTP at MSU is to develop Ph.D. scientists who 
will pursue a research career in medical mycology.  The faculty in the 
Program have varied interests and strengths.  The result is a strong 
interdisciplinary approach to training the predoctoral students.  The 
combined faculty utilize virtually all of the modern research facilities 
on campus which include: DNA/RNA laboratories; computer image analysis 
and phospho imaging systems; hybridoma laboratories; modern microscopic 
techniques; flow cytometry laboratories; and, mass and NMR 
spectrometers.  MSU has a campus-wide computer network and is a major 
node for NSFnet which gives access to supercomputers.
	Ten research faculty from four departments at MSU serve as Ph.D. 
mentors.  Most of the faculty have federally funded research programs and 
include: Clifford W. Bond, virologist; Diane L. Brawner, medical 
mycologist; Jim E. Cutler, medical mycologist and Dir. MMPTP; Gill G. 
Geesey, biofilms; Joan M. Henson, fungal molecular genetics; A.J. 
Jesaitis, neutrophil function and signal transduction; Mark A. Jutila, E- 
and L-selectins, inflammation and gamma/delta T-cells; Mark T. Quinn, 
neutrophil function and inflammation; Norman D. Reed, immunologist; and, 
Gary A. Strobel, fungal pathogens and endophytes, fungal natural products.

MMPTP Training Opportunities:
	In addition to dissertation work, trainees have the following 
opportunities.  Two to three months of clinical mycology laboratory 
training under the direction of Kevin C. Hazen, Ph.D. at the University 
of Virginia Health Sciences Center (UVAHSC).  Dr. Hazen is Associate 
Director of Clinical Microbiology Laboratories and Director of Medical 
Mycology at UVAHSC.  Yearly workshops in clincial mycology at MSU by 
Michael G. Rinaldi, Ph.D., Chief of Clinical Microbiology Laboratories at 
the Univ. Texas Health Sci. Center in San Antonio.  The trainees will 
also take a science core which includes medical and general mycology 
courses at MSU.

Trainee Expenses Provided by the MMPTP:
	Travel expenses will be provided for the trainees to participate 
in clinical mycology laboratory training at UVAHSC and to present their 
research findings each year at a national scientific meeting.  Trainees 
will receive an annual stipend of $14,000 to defray living expenses and 
they will receive fee waivers fro graduate studies.

Openings in the Program and Minimum Criteria for Acceptance:
	We currently have two trainees in the Program and we will have 
two more openings by Fall Semester (September) of 1995.  Applications 
will be accepted until the openings are filled.  Minimum criteria for 
admission are US citizenship, baccalaureate degree in science, GPA 3.0 in 
science courses and Graduate Record Exam combined verbal + quantitative 
score of 1150.  Please note that women and minorities are especially 
encouraged to apply.

Address for Inquiries and Further Information About Admission into the 
Program:
	Jim E. Cutler, Ph.D., Director MMPTP, Department of Microbiology, 
109 Lewis Hall, Montana State University, Bozeman, MT 59717; FAX: 
406-994-2373; INTERNET: umbjc@gemini.oscs.montana.edu.



From SPhysiques@aol.com Sat Feb  4 12:13:00 EST 1995
Article: 1390 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!howland.reston.ans.net!agate!library.ucla.edu!csulb.edu!paris.ics.uci.edu!ucivax!gateway
From: SPhysiques@aol.com
Subject: * A SWEETHEART FOR VALENTINE'S *
Message-ID: <950204073132_12679908@aol.com>
Newsgroups: bionet.mycology
Lines: 65
Date: 4 Feb 95 13:04:50 GMT


        ...........................................

            Valentine's Day will be here soon !

        ...........................................


                      Why give someone

          a boring, heart-shaped box of chocolates

                       when they can

                    EAT YOUR HEART OUT !


o==========================================================o
|                                                          |
| Introducing ...                        ***     ***       |
|                                       ****** ******      |
|               THE                      ***********       |
|                                         *********        |
|      ANATOMICALLY - CORRECT              *******         |
|                                            ***           |
|        CHOCOLATE  HEART                     *            |
|                                                          |
|                                                          |
|  ... a delicious, solid hunk of premium milk chocolate   |
|                                                          |
|   IN THE EXACT SAME SHAPE AND SIZE AS A HUMAN HEART !    |
|                                                          |
o==========================================================o


              FOR PHOTOS AND AN ORDER FORM:

       send an e-mail message to 7272259@MCIMail.com
       ---------------------------------------------

*  Include the word "GIF" if you can view GIF photos

*  Include the word "JPEG" if you can view JPEG photos

   A UUencoded version of two photos will be sent to you.

   The first photo shows the Chocolate Heart with it's
   romantic gift box (perfect for Valentine's Day!)

   The second photo shows the Chocolate Heart with it's
   lookalike cooler (perfect for transplanting your
   amorous organ to a loved one).

   A brief set of instructions will also be sent in case
   you are unfamiliar with UUencoding / UUdecoding.


o-----------------------------------------------------------o
| This Valentine's Day, give a gift straight from the heart |
o-----------------------------------------------------------o


The Anatomically-Correct Chocolate Heart is available only
in the Continental U.S., Alaska, Hawaii, and Puerto Rico.



From gosfield@udcemail.udc.upenn.edu Sat Feb  4 12:13:01 EST 1995
Article: 1391 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!swrinde!howland.reston.ans.net!news.cac.psu.edu!news.pop.psu.edu!psuvax1!news.cc.swarthmore.edu!netnews.upenn.edu!wal6000a.udc.upenn.edu!gosfield
From: gosfield@udcemail.udc.upenn.edu (Edward Gosfield)
Newsgroups: bionet.mycology
Subject: southern mushrooms
Date: 4 Feb 1995 04:29:31 GMT
Organization: University of Pennsylvania
Lines: 13
Message-ID: <3guvrb$f1j@netnews.upenn.edu>
NNTP-Posting-Host: wal6000a.udc.upenn.edu
X-Newsreader: TIN [version 1.2 PL2]

latest sales circular from Edward R. Hamilton, Bookseller, Falls Village,
CT 06031-5000 offers "A Field Guide to Southern Mushrooms" by Smith and
Weber, U of Mich Press, for 9.95.  I have dealt with this firm for years,
and they are trustworthy.  No credit cards, no phone.  Send a check, with
$3.00 extra for shipping, or request the January 27 circular to see what
else you want.  You will end up on their mailing list forever.  I once got
a copy of Bigelow's 'Clitocybe Vol 1' from them for about 9 bucks. 

I have no commercial connection blah blah

ted g
gosfield@udcemail.udc.upenn.edu



From gwhited@genencor.com Sat Feb  4 12:13:02 EST 1995
Article: 1392 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!news-server.ncren.net!news.duke.edu!agate!howland.reston.ans.net!news.sprintlink.net!uunet!hodes.com!netcomsv!gatekeeper.genencor.com!usenet
From: gwhited@genencor.com
Newsgroups: bionet.mycology
Subject: Re: Morel Production
Date: 4 Feb 1995 00:33:40 GMT
Organization: Genencor International, Inc. - (415) 742-7500
Lines: 13
Message-ID: <3gui14$17h@gatekeeper.genencor.com>
References: <3g5pne$qae@newsbf02.news.aol.com>
NNTP-Posting-Host: 10.1.1.41
X-Newsreader: AIR News 3.X (SPRY, Inc.)

>   mycochef@aol.com (MYCOCHEF) writes:
>  try Paul Stammets of Fungi Perfecti in Olympia, 
>  Wash.  call information for the number (area code 206).
>  
>>>>
Full address and phone number:

Paul Stamets
Fungi Perfecti
PO Box 7634
Olympia, WA  98507
(206) 426-9292



From bact@selway.umt.edu Sat Feb  4 20:58:26 EST 1995
Article: 1393 of bionet.mycology
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From: bact@selway.umt.edu (James E Gannon)
Newsgroups: bionet.mycology
Subject: Morphology in submerged culture
Date: 4 Feb 1995 13:38:24 -0700
Organization: University of Montana
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Summary: What Controls morphology in submerged culture?
Keywords: Rhizopus, Morphology, Submerged Culture
X-Newsreader: TIN [version 1.2 PL2]

I have just begun working with submerged (liquid grown) cultures of 
Rhizopus.  Form the same spore prep, some times I get discrete bead like 
morphology and others I get an "uncontrolled" filamentous mass?  What 
specifically controls morphological type under submerged conditions?  
<bact@selway.umt.edu).





From watson@pioneer.arc.nasa.gov Sun Feb  5 20:52:44 EST 1995
Article: 1394 of bionet.mycology
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From: watson@pioneer.arc.nasa.gov (John S. Watson - FSC)
Newsgroups: bionet.mycology
Subject: Honey Mushrooms are Tree killers?
Date: 3 Feb 1995 00:05:08 GMT
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Hello,

Recently the brich in front of my parents house all died,
and around them were a bunch of small muchrooms that looked like
honey mushrooms, ( Armillariella [= Armillaria] mella ).
At a friends house near here I recently found much bigger
honey mushrooms all around 3 of his tree. 

Acording to David Aura's book "Mushroom's Demystified",
honey mushrooms are parasite for which there is no cure,
and which eventually kills the host tree.

So does anyone know anymore about these guys?  Is there
anything that can be done except sit back and enjoy the mushrooms?
So, are all these trees doomed?  How long does it take to kill them?
Should trees killed by them be replaced in the same area, 
and and should we wait a while to do so?

Thanks,
John
John S. Watson                  
NASA Ames Research Center MS/243-9, Moffett Field, CA 94035
<a href="http://ccf.arc.nasa.gov/~watson/watson.html">John S. Watson</a>

"Don't it always seem to go, that you don't know what you've got 'til it's gone ..."


From rarnold@teleport.com Mon Feb  6 23:51:49 EST 1995
Article: 1395 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!howland.reston.ans.net!agate!library.ucla.edu!psgrain!rainrgnews0!news.teleport.com!usenet
From: "Ralph D. Arnold" <rarnold@teleport.com>
Newsgroups: bionet.mycology
Subject: Pleurotus tuber-regium??
Date: Sat, 4 Feb 95 22:41:19 PST
Organization: Teleport - Portland's Public Access (503) 220-1016
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I have read several reports that within Nigeria there exists a type
of mushroom with an underground "tuber" or sclerotia that is edible.
Scientific name is Pleurotus tuber-regium (=Lentinus tuber-regium).
Common names are:
   ofuako (Mid-Western States)
   ohu (Western Nigeria)
   osu (East and Central parts)
   rumbagada (Hausa people of Northern part)
   ufetu (Asaba division of Mid-Western state)
   utun weromon (Ishan division of Mid-Western state)

Literature states that this mushroom "occurs in both tropical and 
subtropical regions of the world.  It...forms large spherical to ovoid, 
subterranean sclerotia which sometimes measures up to 30 cm."

I am seeking a culture or sclerotia of this species.
Any assistance in this matter would be greatly appreciated!

Regards,

Ralph D. Arnold
fax: 1-503-423-7409
rarnold@teleport.com
PO Box 889
Oregon City, OR   97045 USA
voicemail: 1-503-656-4709

------ Forwarded message ends here ------



From Louis.bernier@sbf.ulaval.ca Mon Feb  6 23:51:50 EST 1995
Article: 1397 of bionet.mycology
Newsgroups: bionet.mycology
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From: Louis.bernier@sbf.ulaval.ca (Louis Bernier)
Subject: infection of fungi by protozoa
Content-Type: text/plain; charset=ISO-8859-1
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I was recently contacted by a graduate student in soil microbiology. She is

working on Penicillium spp. she has isolated from the soil and she has
found 
that all her cultures are contaminated with protozoa (probably amebas, she 
believes). She is looking for information on how to get rid of these little

beasts (the protozoa of course, not the fungi !). If any of you has some 
helpful hints, please contact me and I will pass the information on to the 
student.

Thanks for your help.

Louis Bernier
Forest Pathologist
Faculte de foresterie et de geomatique
Pavillon Charles-Eugene Marchand
Universite Laval
Sainte-Foy, Quebec, Canada  G1K 7P4

Phone: 418-656-7655
FAX:418-656-7493
E-mail: Louis.bernier@sbf.ulaval.ca


From mzieg1234@aol.com Fri Feb 10 22:31:25 EST 1995
Article: 1398 of bionet.mycology
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From: mzieg1234@aol.com (MZieg1234)
Newsgroups: bionet.mycology
Subject: Companion culture?
Date: 6 Feb 1995 20:45:42 -0500
Organization: America Online, Inc. (1-800-827-6364)
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Mushroom cultivators are supposed to be secretive about successes but I
would
like to report something interesting. About a month ago I wrote about the
possibility of combining two different mushroom types in the same bag and
called it "companion culture." The idea was that the combination of an
easy
fruiter and difficult fruiter would be -well- fruitful.  The presence of
competition or transfer of chemical/physical factors might induce
primordia
in the recalcitrant species. Nobody responded and I have not seen anything
in
the literature but it seemed a reasonable hypothesis. I have had no luck
with
Grifola frondosa in the past- an occasional aborted primordia. In tests
this
month once again control bags showed no primordia but in 50%(believe me
this
is gigantic improvement) of the bags with two species have nicely
developing
Grifola fruitbodies! Curiously the second species is not producing
fruitbodies?  I am a scientist and know that many more tests need to be
done
and it all could be coincidence. I am currently repeating the tests and
perhaps the results will be different. I am truly hesitant to even send
this
note because of my academic upbringing but wouldn't mind if somebody tried
repeating this on a small scale if they have the time or inclination. It
could be of some importance economically for growing both Grifola and even
other species. Some of you full timers must have tried this?  I can't
quite
bring myself to say what the other species are just yet but two worked and
I
have a feeling it is not too critical.  <:) Mike Ziegler


From neubrain@delphi.com Fri Feb 10 22:31:26 EST 1995
Article: 1399 of bionet.mycology
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From: da5id <neubrain@delphi.com>
Newsgroups: bionet.mycology
Subject: Re: Black Bolete
Date: Tue, 7 Feb 95 00:15:46 -0500
Organization: Delphi (info@delphi.com email, 800-695-4005 voice)
Lines: 4
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X-To: MYCOCHEF <mycochef@aol.com>

just curious about whether there there are indeed any boletes growing
in the northeast and if they are whether they are safe?...
or...could you point us in the direction of  somebody  who would know?
shanjks!


From jdkirkla@prairienet.org Fri Feb 10 22:31:27 EST 1995
Article: 1400 of bionet.mycology
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From: jdkirkla@prairienet.org (Justin D. Kirkland)
Newsgroups: bionet.mycology
Subject: Pluerotus Slide Show
Date: 7 Feb 1995 15:31:54 GMT
Organization: University of Illinois at Urbana
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In my previous posting I said I needed Pluerotus slides for 
a presentation.  I have had a few responses, but I feel I need
to clarify that I am looking for projector slides for a slide
show on Oyster Mushroom production and not microscope slides.
Any help would be appreciated.  Thanks-Justin
-- 


From pwc@MATIES.SUN.AC.ZA Fri Feb 10 22:31:29 EST 1995
Article: 1401 of bionet.mycology
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From: pwc@MATIES.SUN.AC.ZA (Dr PW Crous, Plantpatologie, Tel 4796)
Newsgroups: bionet.mycology
Subject: Hyphomycetes
Date: Tue, 7 Feb 1995 16:47:49 GMT
Organization: University of Stellenbosch, South Africa
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Does anyone know of a good paper illustrating/discussing the mode of 
conidiogenesis of the hyphomycete genera (conidial fungi) Gyrothrix Corda 
and Circinotrichum Nees.  I think several papers suggest they have 
annellations near the apices of the conidiogenous cells.  However, could 
these not be small denticles?  (I ask this because of a weird strain I have 
isolated that appears to have the latter).

On a similar note, does anyone know who erected (and where) the genus 
Vermiculariopsiella.  I suspect its Castaneda & Kendrick (its also recent).

Yours in anticipation
Pedro Crous
PWC@MATIES.SUN.AC.ZA


From stewart@bud.peinet.pe.ca Fri Feb 10 22:31:30 EST 1995
Article: 1402 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!swrinde!howland.reston.ans.net!torn!news.unb.ca!peinet.pe.ca!peinet.pe.ca!not-for-mail
From: stewart@bud.peinet.pe.ca (Paul Stewart)
Newsgroups: bionet.mycology
Subject: cloning from storebought
Date: 7 Feb 1995 15:30:33 -0400
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Keywords: shiitake, oyster

Has anyone successfully established mycelia from grocery store shiitakes 
or oysters?  Nobody on PEI seems to have mycelia in culture (if I'm wrong 
please contact me), and so far I'm too cheap to buy spawn commercially. 
Also, I'm trying to keep it simple so as to eventually sell kits for home 
kitchens using local waste material.  I have managed to start yellow morel 
and chanterelle mycelia from spores, but don't expect to fruit them this 
century :-} . I have just put some oyster stipe inner tissue into malt yeast 
agar with poplar sawdust added...about 50 slants.  So while I'm crossing 
fingers against contamination, please let me know if anybody has tried this.

Seeking not to reinvent the wheel, just grease it...Paul

ABIOGEN c/o Paul Stewart   <stewart@bud.peinet.pe.ca>
RR #2 Vernon Bridge
Prince Edward Island
CANADA   C0A 2E0
(902) 651-3163




From mspear@telerama.lm.com Fri Feb 10 22:31:32 EST 1995
Article: 1403 of bionet.mycology
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From: mspear@telerama.lm.com (mspear)
Newsgroups: bionet.mycology
Subject: Re: Morphology in submerged culture
Date: 6 Feb 1995 11:15:13 -0500
Organization: Telerama Public Access Internet, Pittsburgh, PA
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James E Gannon (bact@selway.umt.edu) wrote:
> I have just begun working with submerged (liquid grown) cultures of 
> Rhizopus.  Form the same spore prep, some times I get discrete bead like 
> morphology and others I get an "uncontrolled" filamentous mass?  What 
> specifically controls morphological type under submerged conditions?  
> <bact@selway.umt.edu).



 
In some species the speed of agitation or ammount of bubbling can have
an effect like you discribe.

    ---- Mark


From stella3771@aol.com Fri Feb 10 22:31:33 EST 1995
Article: 1404 of bionet.mycology
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From: stella3771@aol.com (Stella3771)
Newsgroups: bionet.mycology
Subject: Textbook reviewers wanted (paid)
Date: 7 Feb 1995 16:52:02 -0500
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I work for a science textbook publishing company (Benjamin/Cummings), and
I am looking for college professors who can do some reviewing.  This
involves taking a look at what is in the current textbook and filling out
a couple of forms about it.  I am most interested in having professors who
can review the evolutionary diversity chapters of an introductory biology
book for nonmajors, although I have some openings in other units (plant
physiology, animal physiology, and genetics.)  I have been told by
professors that the average chapter takes about two hours to review, and
we pay $80.00/ chapter.  If you are interested, please contact me by phone
at (800) 950-2665 ext. 887, or by e-mail at TabindaK@bc.aw.com.  *Please*
don't respond to the e-mail address that this message came from.  Thanks.


From mycochef@aol.com Fri Feb 10 22:31:34 EST 1995
Article: 1405 of bionet.mycology
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From: mycochef@aol.com (MYCOCHEF)
Newsgroups: bionet.mycology
Subject: Re: Honey Mushrooms are Tree killers?
Date: 6 Feb 1995 22:43:23 -0500
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Hi,
  go out and harvest those puppies, heat up a pan, separate the stems from
the caps, strip the tough outer layer from the stems and slice the caps,
add some good olive to the pan and saute those shrooms until a little
crispy.
   now enjoy them while watching your trees die.  look under the bark and
you'll see the mycelium threads clinging to the tree and eating three
squares a day.
   some folks get sick at that sight and some just get sick from the
mushroom so be careful when serving large groups.
  as always,
mycochef


From pinto@sfu.ca Fri Feb 10 22:31:35 EST 1995
Article: 1406 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!howland.reston.ans.net!torn!news.bc.net!newsserver.sfu.ca!moorelab.biol.sfu.ca!pinto
From: Linda Pinto <pinto@sfu.ca>
Newsgroups: bionet.mycology
Subject: Blocking protein synthesis
Date: 8 Feb 1995 01:02:12 GMT
Organization: Simon Fraser University
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I am working with a wild type (from soil) Penicillium strain which is
resistant to cycloheximide (500 micrograms/ml). I would like to inhibit
protein synthesis (in liquid culture) to see whether a certain enzyme is
induced. Do you know of an appropriate antibiotic that works with
filamentous fungi, and could you suggest an appropriate concentration?
Thanks for any information! Linda


From VPGH51A@prodigy.com Fri Feb 10 22:31:36 EST 1995
Article: 1407 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!swiss.ans.net!prodigy.com!usenet
From: VPGH51A@prodigy.com (Jim Berlstein)
Newsgroups: bionet.mycology
Subject: Re: Black Bolete
Date: 7 Feb 1995 18:48:45 GMT
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Sure there boletes there.
Some are good, some are bad.
Like always, it is a species by species thing.

I have found boletus edulis in the NE. (once)
Also, I think Boletus bicolor was common and OK.
There are lots of Tylopolus genus boletes, the bitter bolete, not good.

July and August mostly, if I remember correctly



From rpeter@nmsu.edu Fri Feb 10 22:31:37 EST 1995
Article: 1408 of bionet.mycology
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From: rpeter@nmsu.edu (Peter Herman x5495)
Newsgroups: bionet.mycology
Subject: Re: infection of fungi by protozoa
Date: 7 Feb 1995 18:44:34 GMT
Organization: New Mexico State University
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In article <Louis.bernier-060295154722@ophiostoma.rsvs.ulaval.ca> Louis.bernier@sbf.ulaval.ca (Louis Bernier) writes:
>I was recently contacted by a graduate student in soil microbiology. She is
>
>working on Penicillium spp. she has isolated from the soil and she has
>found 
>that all her cultures are contaminated with protozoa (probably amebas, she 
>believes). She is looking for information on how to get rid of these little
>
>beasts (the protozoa of course, not the fungi !). If any of you has some 
>helpful hints, please contact me and I will pass the information on to the 
>student.
>

	There are 2 good ways

1)	Get the cultures sporulating.  Suspend many spores in a
surfactant (like 0.05% Tween 20).  Do serial dilutions of the
spores.  Put a drop on drop of suspension on the plate and spread or
run it around to single spore.  Some of the colonies will be amoeba
free

2)	Make a metal or glass ring with feet (I use 0.5 cm slices of
20 mm pyrex test tubes with 3 mm glass beads welded on to the ring in
3 spots).  Put sterile ring in sterile petri dish.  Pour in agar so
that the agar level is over the feet and about mid-ring.  Inoculate
contaminated culture in the ring when the agar is completely set and
plates are dried. (you have to watch out for condensate).  The
fungus will grow down through the agar and out from under the ring.
cut a bit of the growing front when it is 2-3 cm beyond the ring.
You may need to repeat the process.  Bacteria and protozoa stay
inside the ring because they cannot penetrate the agar.

I use this to clean up field isolates of watermolds all the time and
it works like a charm.  Thank yous to John R. Raper who thought of
this technique eons ago!

Hope this helps


*   Peter Herman, Dept. of Biology	Phone: +1 505 646 4532    *
*   New Mexico State University		Fax:   +1 505 646 5665    *
*   Box 30001, Dept. 3AF					  *
*   Las Cruces, NM 88003 USA		e-mail rpeter@nmsu.edu    *


From rwk@sylvanres.com Fri Feb 10 22:31:38 EST 1995
Article: 1409 of bionet.mycology
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From: Richard W. Kerrigan <rwk@sylvanres.com>
Newsgroups: bionet.mycology
Subject: Re: mycorhizal fungi
Date: 7 Feb 1995 12:20:03 -0500
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Paul Stewart (stewart@bud.peinet.pe.ca) wrote:
: My sincere thanks to all who have responded so far to my inquiries into 
: mycorrhizal culture...

[ETC.]

I didn't see the following info posted, so:  The research lab 'Station 
Champignons' at INRA-Bordeaux has pioneered this area very successfully 
for more than 15 years.  Contact Dr. J.-M. Olivier at the station, BP 81, 
33883 Villenave d'Ornon, FRANCE.  They wrote the book on Truffle culture 
and have had other successes, e.g. Lactarius spp.

You might also want to contact Dr. Makoto Ogawa of Japan, to learn about 
unsuccessful work on Matsutake.  I don't have his address at hand but 
could look it up if you e-mail me directly at rwk@sylvanres.com.

Good luck! -- Rick
-- 
Richard W. Kerrigan, Research Department, Sylvan Spawn Laboratory, Inc.		
               1163 Winfield Rd., Cabot, PA  16032  USA
e-mail:	rwk@sylvanres.com      phone: 412-352-1521      fax: 412-352-4062
	"Success has a thousand parents; failure is an orphan"


From tjvolk@facstaff.wisc.edu Fri Feb 10 22:31:40 EST 1995
Article: 1410 of bionet.mycology
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From: tjvolk@facstaff.wisc.edu (Tom Volk)
Newsgroups: bionet.mycology
Subject: Re: Honey Mushrooms are Tree killers?
Date: Wed, 8 Feb 1995 16:10:24 GMT
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Distribution: na
Message-ID: <tjvolk.73.2F38ECEF@facstaff.wisc.edu>
References: <3grrvk$ltt@onramp.arc.nasa.gov>
NNTP-Posting-Host: f181-170.net.wisc.edu

Hi John. This is with regard to your Armillaria problem.

In article <3grrvk$ltt@onramp.arc.nasa.gov> watson@pioneer.arc.nasa.gov (John S. Watson - FSC) writes:

>Recently the brich in front of my parents house all died,
>and around them were a bunch of small muchrooms that looked like
>honey mushrooms, ( Armillariella [= Armillaria] mella ).
>At a friends house near here I recently found much bigger
>honey mushrooms all around 3 of his tree. 
>Acording to David Aura's book "Mushroom's Demystified",
>honey mushrooms are parasite for which there is no cure,
>and which eventually kills the host tree.
>So does anyone know anymore about these guys?  Is there
>anything that can be done except sit back and enjoy the mushrooms?
>So, are all these trees doomed?  How long does it take to kill them?
>Should trees killed by them be replaced in the same area, 
>and and should we wait a while to do so?

As you probably know, what we used to call Armillaria mellea has been split 
up into 9-10 species of Armillaria in this country (aboutt 35-40 species 
worldwide).  These are defined by matings as biological species.  This is 
certaily not a purely academic exercise fromt he standpoint of patholoy.  
The different species vary widely in their pathogenicity and many are specif 
to hardwood or conifer.  For example true Armilllaria mellea (Vahl:Fr) 
Kummer can be e sever pathogen of hardwoods, and doesn't occur much on 
conifer.  Armillaria gallica Marxm. & Romagn (= A.bulbosa Velen.) is 
probably the most common species in the east, and is not very pathogenic.  
Evidence indicate it is probably just saprophytic.  Armillaria ostoyae 
(Romagn.) Herink is normally a severe pathogen of conifers, but also occurs 
nearly half the time on hardwoods.
I can't tell for sure from your post, but I think you;re in California.  One 
study in California has addressed this problem.
Jacobs, K.A. et al  Identification of Armillaria species in California.  
Mycologia 86:113-116, 1994
Much more work needs to be done on pathogenicity of the various species in 
different parts of the country.
What is clear, however, is that your birch tree is doomed.  Once Armillaria 
takes hold, the rhizomorps and mycelial fans penetrate under the bark of the 
roots and the "butt " of the tree and destroy the cambial layer, everntually 
girdling the tree.
As MycoChef pointed out, though, they are delicious.  I have noted no real 
difference in flavor in any of the species, although you certainly want to 
cook them *very* well in any case.  there are several reports of illness 
from eating undercooked Armillarias.
This may be much more than you wanted to know, but it's kind of interesting 
to me-- I am in volved in writing a monograph of the North temperate species 
of Armillaria at present.  Fell free to write if you have queestions.
---Tom
Tom Volk, Center for Forest Mycology research, Forest Products Lab, Forest 
Service, Madison Wisconsin   tjvolk@facstaff.wisc.edu


From REA105@psuvm.psu.edu Fri Feb 10 22:31:40 EST 1995
Article: 1411 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!rutgers!gatech!newsfeed.pitt.edu!hudson.lm.com!news.pop.psu.edu!news.cac.psu.edu!psuvm!rea105
Organization: Penn State University
Date: Tue, 7 Feb 1995 11:08:18 EST
From: Klamachpin Achgeketum <REA105@psuvm.psu.edu>
Message-ID: <95038.110818REA105@psuvm.psu.edu>
Newsgroups: bionet.mycology
Subject: United Kingdom and Deutschland
Lines: 8

     I am a University Scholar at Penn State University.  I am currently workin
g on a VCG analysis of V. fungicola.  I am also in the initial stages of fillin
g out the applications for the Marshall(UK) and the Fulbright(Deut.) scholarshi
ps.  Eventually I will need to decide at which institution I would like to cont
inue my studies.  It is my hope that I will be able to find a location where my
 skills may be of use.  If you have any suggestions, or would like to here more
 information, please contact me by e-mail at REA105@psuvm.psu.edu.  Many Thanks
Ryan Argot


From kcowing@clark.net Fri Feb 10 22:31:42 EST 1995
Article: 1412 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!howland.reston.ans.net!news.sprintlink.net!news.clark.net!kcowing-ppp.clark.net!user
From: kcowing@clark.net (Keith L. Cowing)
Newsgroups: bionet.biology.grasses,bionet.genome.arabidopsis,bionet.mycology,bionet.photosynthesis,bionet.plants
Subject: UPDATE: Call for Proposals: NASA Ground-Based and Small Payloads Research in Space Life Sciences
Date: Wed, 08 Feb 1995 11:48:59 -0500
Organization: American Institute of Biological Sciences
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Xref: bigblue.oit.unc.edu bionet.biology.grasses:62 bionet.genome.arabidopsis:2761 bionet.mycology:1412 bionet.photosynthesis:530 bionet.plants:5120

UPDATE: NASA NRA OLMSA 95-01 Now Available For Downloading

The following NASA Research Announcement has been mailed (1/26/95) to a
standard NASA distribution list.  This NRA can be obtained by contacting
the NASA officials at the email or postal addresses listed below.  

You can now download this entire NRA from the NASA OLMSA Life Sciences
Gopher at NASA Headquarters (for Mac and PC) by selecting "Division Wide
NRA" at either:

       lifegopher.hq.nasa.gov

or via WWW at:

       gopher://lifegopher.hq.nasa.gov/



+++++++++++++

NASA OMB Approval No. 2700-0042
NRA 95 OLMSA-01
NASA Research Announcement Soliciting Proposals for 
Ground-Based and Small Payloads Research in Space Life Sciences

Life and Biomedical Sciences and Applications Division
Office of Life and Microgravity Sciences and Applications
National Aeronautics and Space Administration

Date NRA Issued:  January 26, 1995
Letters of Intent Due:  March 24, 1995
Proposals Due:  April 21, 1995

This National Aeronautics and Space Administration (NASA) Research
Announcement (NRA) solicits proposals to participate in Ground-Based and
Small Payloads Research in Space Life Sciences.  This is a broad-based
announcement that solicits research proposals for all of the major
programs of the Life and Biomedical Sciences and Applications Division. 
The specific programs that are included in this announcement are Space
Biology, Space Physiology and Countermeasures, Environmental Health, Space
Radiation Health, Space Human Factors, Advanced Life Support, Advanced
Extravehicular Activity Systems, Advanced Technology Development and Data
Analysis.  Proposals submitted in response to this Announcement may be
either for standard ground-based research investigations or for small
payload flight experiments or for both.  Proposal to develop experiments
aiming at space station utilization are particularly encouraged.

Subsequent announcements similar to and based upon this Announcement will
be updated and issued annually and will be the primary means of obtaining
research proposals form the scientific community for ground and small
payloads research in the space life sciences.  Although this NRA is
broad-based, it is restricted to the science programs named above and
described in detail in Appendix A.  The potential proposer should read the
program descriptions that are of interest with care and attempt to focus
the proposal on the specific research emphases defined in this
Announcement.

Participation in the Ground-based and Small Payloads Research in Space
Life Sciences is open to all individuals and all categories of domestic
and foreign organizations, industry, educational institutions, other
nonprofit organizations, NASA laboratories, and other U.S. Governmental
agencies.  Proposals which will enhance or complement the scientific
return from research currently being supported by the National Institutes
of Health, National Science Foundation, or other Government agencies are
encouraged.  

In addition, proposals to advance technology and develop practical
applications of technology are sought under this Announcement.  Because of
limitations of access to flight opportunities, it is expected that the
majority of proposals approved in response to this Announcement would be
for ground-based research.  

A letter of Intent to Propose is requested by March 24, 1995.  Proposals
may be submitted at any time up to April 21, 1995.  Proposals will be
evaluated for scientific/technical value, soundness, intrinsic
scientific/technical value, relevance, implementation feasibility, and
cost.  A selection announcement will be made between August and October of
1995.  Funding of selected proposals will begin sometime between October
1995 and September 1996.


Proposals due:   April 21, 1995
Letters of Intent Due:  March 24, 1995

Number of copies Requested:  20

NASA Selecting official:

     Dr. Joan Vernikos
     Director, Life and Biomedical Sciences and Applications   
     Division/ Code ULR
     NASA Headquarters
     Washington, DC 20564-0001

Obtain additional information from the appropriate Science Program
manager, as indicated below, at the following address:

     UL/ Life and Biomedical Sciences 
     and Applications Division
     NASA Headquarters
     Washington, DC 20546-0001
     Telephone: 202-358-2530
     Fax: 202-358-4168

Program: Program Manager/Email

Space Biology: 
  Dr. Mary Anne Frey
  MAFrey@smtpgmgw.olmsa.hq.nasa.gov

Space Physiology and Countermeasures:
  Dr. Victor S. Schneider
  vschneider@smtpgmgw.olmsa.hq.nasa.gov

Environmental Health:
  Dr. Victor S. Schneider
  vschneider@smtpgmgw.olmsa.hq.nasa.gov

Space Radiation Health:
  Dr. Frank M. Sulzman 
  fsulzman@smtpgmgw.olmsa.hq.nasa.gov

Space Human Factors:
  Dr. Guy C. Fogleman
  GCFogleman@smtpgmgw.olmsa.hq.nasa.gov

Advanced Life Support:
  Dr. Maurice M. Averner
  maverner@smtpgmgw.olmsa.hq.nasa.gov

Advanced Extravehicular Activity Systems:
  Mr. Robert K. Callaway
  RCallaway@smtpgmgw.olmsa.hq.nasa.gov

Advanced Technology Development:
  Mr. Gregory K. Schmidt
  gschmidt@smtpgmgw.olmsa.hq.nasa.gov

Data Analysis:
  Dr. Ronald J. White
  RJWhite@smtpgmgw.olmsa.hq.nasa.gov

Small Payloads Program:
  Mr. Marc Shepanek
  mshepanek@smtpgmgw.olmsa.hq.nasa.gov


Proposals and Letters of Intent mailed through the U.S. Postal Service by
express, first class, registered, or certified mail are to be sent to the
following address:

    Ronald J. White, PhD.
    Life and Biomedical Sciences and Applications   
       Division/ Code UL
    NASA Headquarters
    Washington, DC 20546-0001

Proposals and Letters of Intent hand delivered or sent by commercial
delivery or courier services are to be delivered between the hours of 8 AM
and 4:30 PM:

    Ronald J. White, PhD.
    Life and Biomedical Sciences and Applications   
      Division/ Code UL
    National Aeronautics and Space Administration
    ATTN: Receiving and Inspection (Rear of Building)
    300 E street, SW
    Washington, DC 20024-3210

The telephone number 202-488-2940 may be used when required for reference
by delivery services.  NASA cannot receive deliveries on Saturdays,
Sundays, or federal Holidays.

Special instructions apply to foreign (non-U.S.) proposals.


From nora@molbio.cbs.umn.edu Fri Feb 10 22:31:43 EST 1995
Article: 1413 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!news-server.ncren.net!news.duke.edu!godot.cc.duq.edu!newsfeed.pitt.edu!gatech!taco.cc.ncsu.edu!biosci!MANI.CBS.UMN.EDU!npv
From: npv@MANI.CBS.UMN.EDU (Nora Plesofsky-Vig)
Newsgroups: bionet.mycology
Subject: calcium in fungi
Date: 7 Feb 1995 12:54:08 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
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I would appreciate some help in designing/assessing experiments to  
determine cytoplasmic calcium levels in Neurospora crassa or another  
fungus. Specific questions are: 

	(1) Does the esterified fura-2 work in Neurospora for  
fluorescent measurements of calcium, or what are the drawbacks to its  
use in fungi? 

	(2) Does the calcium ionophore ionomycin work in Neurospora,  
or is there a better alternative?

Any handy literature references would also be appreciated.

Nora Plesofsky-Vig  


From rwinder@PFC.Forestry.CA Fri Feb 10 22:31:44 EST 1995
Article: 1414 of bionet.mycology
Newsgroups: bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!howland.reston.ans.net!vixen.cso.uiuc.edu!news.uoregon.edu!netnews.nwnet.net!news.pfc.forestry.ca!PFC.Forestry.CA!RWINDER
From: rwinder@PFC.Forestry.CA (Richard Winder)
Subject: Re: Companion culture?
X-Nntp-Posting-Host: pfc.pfc.forestry.ca
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Sender: news@news.pfc.forestry.ca (0000-news(0000))
Reply-To: rwinder@PFC.Forestry.CA
Organization: Forestry Canada (Pacific Forestry Centre)
References: <3h6jc6$mbh@newsbf02.news.aol.com>
Date: Tue, 7 Feb 1995 22:30:20 GMT
Lines: 46

In article <3h6jc6$mbh@newsbf02.news.aol.com>, 
mzieg1234@aol.com (MZieg1234) writes:
>Mushroom cultivators are supposed to be secretive about successes but I
>would like to report something interesting. About a month ago I wrote about the
>possibility of combining two different mushroom types in the same bag and
>called it "companion culture." The idea was that the combination of an
>easy fruiter and difficult fruiter would be -well- fruitful.  The presence of
>competition or transfer of chemical/physical factors might induce
>primordia in the recalcitrant species. Nobody responded and I have not seen anything
>in the literature but it seemed a reasonable hypothesis. I have had no luck
>with Grifola frondosa in the past- an occasional aborted primordia. In tests
>this month once again control bags showed no primordia but in 50%(believe me
>this is gigantic improvement) of the bags with two species have nicely
>developing Grifola fruitbodies! Curiously the second species is not producing
>fruitbodies?  I am a scientist and know that many more tests need to be
>done and it all could be coincidence. I am currently repeating the tests and
>perhaps the results will be different. I am truly hesitant to even send
>this note because of my academic upbringing but wouldn't mind if somebody tried
>repeating this on a small scale if they have the time or inclination. It
>could be of some importance economically for growing both Grifola and even
>other species. Some of you full timers must have tried this?  I can't
>quite bring myself to say what the other species are just yet but two worked and
>I have a feeling it is not too critical.  <:) Mike Ziegler

    I imagine Paul Stamets will have something to say about this.  It is
 pretty well known that bacteria, when introduced at the proper time, can help
 promote fruiting body development of cultivated mushrooms.  The fungal
 yeast Rhodotorula glutinus has been used to trigger basidiospore
 germination for various mushrooms.  Bacteria have been implicated as
 symbionts with Chantrelles.  There are people I know who hope, some day,
 to study the whole succession of fungi that attack tree stumps, and how they
 interact with each other.  So your findings aren't out of line- there are 
 probably all kinds of interactions going on.  Many multiple-fungus cultures 
 exhibit various degrees of antagonism- and competitive stress
 leading to the formation of sclerotia or fruiting bodies is one of the
 effects.  In producing morel primordia on agar plates, I have noticed
 that contamination by the fungus Fusarium, if it occurs at such a point
 that the whole culture is not swamped with contamination, seems to
 enhance the development of larger sclerotia and primordia.  I know you were 
 hoping for mushrooms for both fungi, but I think its great you've got at 
 least one of them going.
 -RSW

  RICHARD WINDER                    Title: Research Scientist
  Canadian Forest Service           Phone: (604) 363-0773
  Victoria, B.C.                    Internet: RWINDER@A1.PFC.Forestry.CA


From mattman@LELAND.STANFORD.EDU Fri Feb 10 22:31:45 EST 1995
Article: 1415 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!news-server.ncren.net!news.duke.edu!godot.cc.duq.edu!hudson.lm.com!news.pop.psu.edu!news.cac.psu.edu!howland.reston.ans.net!spool.mu.edu!olivea!biosci!LELAND.STANFORD.EDU!mattman
From: mattman@LELAND.STANFORD.EDU (Matt Springer)
Newsgroups: bionet.mycology
Subject: Submerged culture
Date: 6 Feb 1995 12:10:27 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 10
Sender: daemon@net.bio.net
Distribution: world
Message-ID: <199502062010.MAA10779@elaine17.Stanford.EDU>
NNTP-Posting-Host: net.bio.net

Regarding morphology in submerged culture:

I don't know about Rhizopus specifically, but in Neurospora, the
concentration of either carbon or nitrogen source can make the difference
between mycelial growth and short, stunted conidiophore formation
(microcycle conidiation).  Temperature shifts and light can also have an
effect.  A computer search of "microcycle" will give several of these
references.

-Matt Springer


From akiyama@MOL.F.U-TOKYO.AC.JP Fri Feb 10 22:31:46 EST 1995
Article: 1416 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!howland.reston.ans.net!spool.mu.edu!olivea!biosci!MOL.F.U-TOKYO.AC.JP!akiyama
From: akiyama@MOL.F.U-TOKYO.AC.JP
Newsgroups: bionet.mycology
Subject: Expression of bigger protein than expected in E. coli
Date: 8 Feb 1995 19:37:55 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 15
Sender: daemon@net.bio.net
Distribution: world
Message-ID: <199502090337.MAA08211@mol.f.u-tokyo.ac.jp>
NNTP-Posting-Host: net.bio.net

Has anyone ever experienced the production of bigger polypeptide than
expected while expressing protein in E. coli ?
I expressed a plant enzyme in E. coli using pET system. I ligated my cDNA
into the vector pET-3d just at the point of initiation codon, but E. coli
horboring this plasmid produced two different polypeptide. They differ in
size by about 1-2 kDa. Both react to the antibody against the original
plant protein. 
First, I thought this was caused by protease (i.e. the bigger one is the
native form). But I found that the "smaller" one has catalytic activity and
the "bigger" doesn't. Now I assume the presence of  additional unexpected
amino acid sequence before the N-terminus or after the C-terminus.
Has anyone encountered or heard of such situation ? If you have, please
give me a good advise. (I am going to determine the N-terminal amino acid
sequence of both polypeptide in the near future.)



From okellyc@megasun.BCH.UMontreal.CA Fri Feb 10 22:31:47 EST 1995
Article: 1417 of bionet.mycology
Newsgroups: bionet.mycology,bionet.parasitology,bionet.plants,bionet.biology.grasses
Path: bigblue.oit.unc.edu!concert!rutgers!uwm.edu!vixen.cso.uiuc.edu!howland.reston.ans.net!news.sprintlink.net!uunet!newsflash.concordia.ca!CC.UMontreal.CA!megasun!okellyc
From: okellyc@megasun.BCH.UMontreal.CA (Charles J. O'Kelly)
Subject: Taxonomic Research - PEET White Paper Available on WWW
Message-ID: <D3pIIK.D8z@cc.umontreal.ca>
Followup-To: bionet.protista
Keywords: taxonomy, systematics, NSF, funding, PEET
Sender: okellyc@megasun (Charles J. O'Kelly)
Organization: OGMP- Organellar Genome Megasequencing Project
Date: Thu, 9 Feb 1995 00:25:31 GMT
Lines: 79
Xref: bigblue.oit.unc.edu bionet.mycology:1417 bionet.parasitology:459 bionet.plants:5134 bionet.biology.grasses:63

==============================================================================

GUIDELINES FOR MONOGRAPHIC RESEARCH PROPOSALS TO THE PEET PROGRAM OF NSF
                 AVAILABLE ON WORLD WIDE WEB

======================================================================

The National Science Foundation has released a White Paper on the PEET
(Partnerships for Enhancement of Expertise in Taxonomy) program.

The report is titled:


            TAXONOMIC EXPERTS, SYSTEMATIC MONOGRAPHS

         REPORT OF A PANEL-WORKSHOP, 29-30 NOVEMBER 1994
        ON PRACTICES AND DIRECTIONS IN MONOGRAPHIC RESEARCH


and contains a number of specific comments and recommendations for
both writers and reviewers of PEET proposals.

The document may be found at the following URL address:

    http://megasun.bch.umontreal.ca/isep/peet.html

and it may also be accessed from the ISEP home page

    http://megasun.bch.umontreal.ca/isep/isep.html

by selecting the line "PEET Report".

The report is also available by email from okellyc@bch.umontreal.ca
(Charles J. O'Kelly), if you don't have access to the World Wide Web.

If you are not familiar with the PEET initiative, the full description 
may be found in document NSF 94-109 from the Directorate
of Biological Sciences, Division of Environmental Biology, (US)
National Science Foundation. 

"The National Science Foundation (NSF), in partnership with
academic institutions, botanical gardens, freshwater and marine
institutes, and natural history museums, seeks to enhance and
stimulate taxonomic research ... NSF announces a special
competition ... to support competitively reviewed research
projects that target groups of poorly known organisms. ...  Three
major elements are required of a project submitted ...: 1.
Monographic Research; 2. Training; and 3. Computer
Infrastructure.

The deadline for receipt of proposals is 1 March 1995.

Further information:
     Division of Environmental Biology (PEET)
     National Science Foundation
     4201 Wilson Boulevard, suite 635
     Arlington, VA  22230
     USA
          tel: (1) 703 306 1481
          fax: (1) 703 306 0367
          email: sysrev@nsf.gov

To get the whole document by electronic mail, mail to:
     
     stisserve@nsf.gov

Leave the subject line blank.

Send the following message:

     get nsf94109

==========================

charley o'kelly
mad protistologist
okellyc@bch.umontreal.ca




From wylie@CSUVAX1.MURDOCH.EDU.AU Fri Feb 10 22:31:48 EST 1995
Article: 1418 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!hearst.acc.Virginia.EDU!portal.gmu.edu!europa.eng.gtefsd.com!news.umbc.edu!haven.umd.edu!purdue!gatech!howland.reston.ans.net!spool.mu.edu!olivea!biosci!CSUVAX1.MURDOCH.EDU.AU!wylie
From: wylie@CSUVAX1.MURDOCH.EDU.AU (Steve Wylie)
Newsgroups: bionet.mycology
Subject: PAL induced by R. solani
Date: 8 Feb 1995 21:30:54 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 11
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Distribution: world
Message-ID: <Pine.3.87.9502091321.A1158-0100000@csuvax1>
NNTP-Posting-Host: net.bio.net

I have tested several methods to analyse PAL activity from whole 
plants(tissue cultured tobacco) whose defense mechanisms have been 
induced by the eliciter Rhizoctonia solani.I have observed different PAL 
activity in the plants when inoculated with different eliciter 
concentrations. However, I have been unable to quantify PAL activity
by any of the means tested. The methods I have tried are those of 
Bhattacharyya & Ward(1988) and Hillis & Ishikura(1970). Any suggestions 
would be most welcome.
Steve Wylie
wylie@murdoch.edu.au



From S.Leangon@massey.ac.nz Fri Feb 10 22:31:49 EST 1995
Article: 1419 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!howland.reston.ans.net!spool.mu.edu!olivea!biosci!massey.ac.nz!S.Leangon
From: S.Leangon@massey.ac.nz ("S. Leangon")
Newsgroups: bionet.mycology
Subject: How to break fungal mycelium
Date: 8 Feb 1995 22:51:49 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 15
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Distribution: world
Message-ID: <9502090650.AA02832@cc-server4.massey.ac.nz>
NNTP-Posting-Host: net.bio.net

I have 2 questions, any suggestions would be great appreciated.

1.   Does anybody know the good procedure to break the fungal mycelium ?

I  am  working with Aspergillus niger.  I grow it on the surface of a semi-
solid  medium  prepared  from sweet potato root.  The mycelium sheet of the 
old culture is rather hard to break in the porcelain mortar.

2.    I  am  trying  to  assay ATP, AMP, NAD and NADH in this fungus.  Does 
anyone  ever  experienced with this assays (especially in fungal mycelium).  
At  the moment I follow the procedures from "Bergmeyer Methods of Enzymatic 
Analysis" but I am wondering if there are any modifications or cautions.

Please contact me at :
"S.Leangon@massey.ac.nz"


From madamso2@metz.une.edu.au Fri Feb 10 22:31:50 EST 1995
Article: 1420 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!swrinde!howland.reston.ans.net!news.cac.psu.edu!news.tc.cornell.edu!caen!msunews!harbinger.cc.monash.edu.au!news.cs.su.oz.au!metro!news.une.edu.au!usenet
From: Michael Adamson <madamso2@metz.une.edu.au>
Newsgroups: bionet.mycology
Subject: Looking for pathogenic Pleurotus
Date: 9 Feb 1995 00:21:50 GMT
Organization: UNE
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Message-ID: <3hbn6u$kdm@grivel.une.edu.au>
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X-UserAgent: Nuntius v1.0

I recently heard from a FOAF that someone had isolated a Pleurotus from
rotting tubers of Cyperus sp.
Anyone have any idea what species of Pleurotus might be responsible?


From gsmay@bcm.tmc.edu Fri Feb 10 22:31:51 EST 1995
Article: 1422 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!howland.reston.ans.net!agate!biosci!bcm!usenet
From: gsmay@bcm.tmc.edu (Gregory May)
Newsgroups: bionet.mycology
Subject: Abstract
Date: 9 Feb 1995 14:54:21 GMT
Organization: Baylor College of Medicine
Lines: 41
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The following is an abstract of a paer that is in press.  It will appear in
the Journal of Cell Biology Volume 128, No. 4, February 1995.

myoA of Aspergillus nidulans encodes an essential myosin I 
required for secretion and polarized growth.

Carol A. McGoldrick, Carol Gruver and Gregory S. May

ABSTRACT 
	We have identified and cloned a novel essential myosin I in 
Aspergillus nidulans, called myoA. The 1249 amino acid predicted 
polypeptide encoded by myoA is most similar to the amoeboid myosins
 I. Using affinity purified antibodies against the unique myosin I carboxyl
 terminus, we have determined that MYOA is enriched at growing 
hyphal tips. Disruption of myoA, by homologous recombination, resulted
 in a diploid strain heterozygous for the myoA gene disruption. We can 
recover haploids with an intact myoA gene from these strains but never 
haploids that are myoA disrupted. These data indicate that myoA 
encodes an essential myosin I and this has allowed us to use a unique 
approach to studying myosin I function. We have developed conditionally 
null myoA strains in which myoA expression is regulated by the alcA 
alcohol dehydrogenase promoter. A conditionally lethal strain germinated 
on inducing medium grows as wild type, displaying polarized growth by 
apical extension. However, growth of the same myoA mutant strain on 
repressing medium results in enlarged cells incapable of hyphal extension 
and these cells eventually die. Under repressing conditions, this strain 
also displays reduced levels of secreted acid phosphatase. The mutant 
phenotype indicates that myoA plays a critical role in polarized growth 
and secretion.

===============================================
  Gregory S. May			      	
  Department of Cell Biology			
  Baylor College of Medicine			
  One Baylor Plaza				
  Houston, TX  77030				
  Tel.  713-798-4756				
  FAX.  713-798-7799				
  E-mail gsmay@bcm.tmc.edu			
===============================================


From bmiller@uidaho.edu Fri Feb 10 22:31:52 EST 1995
Article: 1423 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!swrinde!howland.reston.ans.net!vixen.cso.uiuc.edu!news.uoregon.edu!gaia.ucs.orst.edu!news.uidaho.edu!xslip07.csrv.uidaho.edu!user
From: bmiller@uidaho.edu (Bruce L. Miller)
Newsgroups: bionet.mycology
Subject: Faculty Position Available
Date: Fri, 03 Feb 1995 10:14:00 -0800
Organization: University of Idaho
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Assistant Professor of Molecular Biology and Biochemistry:  The University
of Idaho is seeking to fill a tenure track position for an assistant
professor.  We are interested in candidates capable of establishing a
nationally competitive research program to study the molecular biology of
plants or fungi in one of two areas: 1) molecular mechanisms of
development  or 2) responses to abiotic and biotic stimuli.  The research
program should complement existing departmental programs in plant
molecular biology and biochemistry, microbial developmental genetics,
medical/pathogenic microbiology and environmental microbiology.  The
successful candidate must have a strong commitment to teaching excellence
and will be responsible for a one semester undergraduate
lecture/laboratory course in basic biochemistry to be taught each year and
a graduate level course in his/her area of expertise in alternate years. 
Applicants should have a Ph.D. in Molecular Biology, Biochemistry or
related field and postdoctoral research experience using contemporary
molecular techniques.  Send CV, statement of research interests and three
letters of reference by March 24, 1995 to Bruce L. Miller, Search
Committee Chair, Department of Microbiology, Molecular Biology and
Biochemistry, Life Sciences Bldg., University of Idaho, Moscow, ID 83844.
FAX (208) 885-6518.  AA/EOE

-- 
Bruce L. Miller
Dept. of Microbiology, Molecular Biology & Biochemistry
University of Idaho
Moscow, ID  83843


From spencal@NEXTLAB.CALSTATELA.EDU Fri Feb 10 22:31:53 EST 1995
Article: 1424 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!howland.reston.ans.net!spool.mu.edu!olivea!biosci!NEXTLAB.CALSTATELA.EDU!spencal
From: spencal@NEXTLAB.CALSTATELA.EDU (Steve Pencall)
Newsgroups: bionet.mycology
Subject: Comment on Forest Service Collecting policy
Date: 9 Feb 1995 12:36:24 -0800
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TO:   ALL AMATEUR AND PROFESSIONAL MYCOLOGISTS
SUBJECT:  Forest Service Comment Period to close February 15!

The COMMENT PERIOD on the U.S. Forest Service's Draft Strategy For  
Special Forest Product Resources closes on February 15. The Draft  
Strategic Plan on Special Forest Product Resources is expected to  
become a guideline for local Forest policies on the collection of  
mushrooms and a host of other non-timber forest products.
 Therefore, it is vitally important for all mushroom collectors who  
collect in National Forests, which is most of us, to have their say  
as to how mushroom picking will be regulated.  If we don't speak up  
on our own behalf, we risk having the policy being determined by a  
few anti-picking extremists.
Unfortunately, throughout the entire development of this Plan, the  
Forest Service has made little or no effort to involve the public in  
formulation of the Plan, including the tens of thousands nationwide  
who gather and use "Special Forest Products".  I am unaware of any of  
the approximately 75 mycological societies having been contacted  
about the Plan, nor any individual members of those societies, nor  
any of the publications which address this audience.  Nor am I aware  
of any other affected groups that have been contacted either.  The  
Plan has not even been published in The Federal Register!
The draft Special Forest Products Plan was finally published on  
October 27 with an allotted comment period of only 27 days!--far too  
little time for most of the affected groups to inform most of their  
members of the opportunity to comment on the policy.  It is difficult  
to escape the conclusion that the reason for the actions that I have  
related is to suppress public input to the Plan.  These actions are  
in direct contradiction of the avowed goals of Forest Service  
"reinvention".
It seems unlikely that anyone in the mycological community would be  
aware of the Plan even today, were it not for a chance remark made to  
a member of the Mycological Society of San Francisco by a staffer in  
the Regional Office of the Forest Service in San Francisco.  I wonder  
if the Forest Service will attempt to discover and punish the person  
who "leaked" word of this Plan?
At the time the Plan was first published in October, the public  
comment period was a mere 27 days!  Now due to protests by this  
writer and others, the comment deadline has been extended an  
additional 12 weeks, to February 15, 1995.
You are urged to write or fax the Forest Service and tell them that  
the Special Forest Products Plan needs additional changes to fairly  
address the concerns of amateur mushroom collectors.  For a list of  
specific points to include in your letter, please contact this  
writer.

Mr. Mike Higgs
USDA Forest Service, CF
4SE AUD
P.O. Box 96090
Washington, DC 20090
Phone: (202) 273-4754
Fax: (202) 205-0975
Office hours are 8 am to 5 pm Eastern Time
Please state that you are commenting on the Draft Strategic Plan for  
Managing Special Forest Product Resources.  You should also request a  
copy of the Plan.


From jolivas@CYBERGATE.COM Fri Feb 10 22:31:55 EST 1995
Article: 1425 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!swrinde!howland.reston.ans.net!spool.mu.edu!olivea!biosci!CYBERGATE.COM!jolivas
From: jolivas@CYBERGATE.COM (John L. Olivas)
Newsgroups: bionet.mycology
Subject: Re: Taxus
Date: 9 Feb 1995 14:07:18 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
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          I have information on Taxus and Arctostaphylos for the people that 
were interested.  However, the address I have is incorect. Email me back 
with a deliverable address.

John Olivas
jolivas@cybergate.com



From claudes@ESSEX.HSC.COLORADO.EDU Fri Feb 10 22:31:56 EST 1995
Article: 1426 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!howland.reston.ans.net!spool.mu.edu!olivea!biosci!ESSEX.HSC.COLORADO.EDU!claudes
From: claudes@ESSEX.HSC.COLORADO.EDU (Claude Selitrennikoff)
Newsgroups: bionet.mycology
Subject: Re: <ad> GUARANTEED CREDIT REPAIR BY LAW FIRM
Date: 9 Feb 1995 16:06:14 -0800
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Dear Credit Advocates:

I object strongly to your advertising on this network.  This is not 
appropriate and I find this behavior highly objectionable.

Claude P. Selitrennikoff, Ph.D.
Professor


From MZieg1234@aol.com Fri Feb 10 22:31:57 EST 1995
Article: 1427 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!howland.reston.ans.net!spool.mu.edu!olivea!biosci!aol.com!MZieg1234
From: MZieg1234@aol.com
Newsgroups: bionet.mycology
Subject: re:cloning from storebought
Date: 9 Feb 1995 20:37:26 -0800
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Paul- It certainly is possible to clone from storebought! My first Shitake
cultures were from the grocery and fruited just fine on sawdust substrate.
Shitakes keep well so I think your chances are better with them than Oysters.
The Oysters I find in the stores are usually slimy and ugh.  Although
obtaining cultures this way is fine you may determine later that you want to
know more about the fruiting characteristics prior to innoculating lots of
logs. Multiyear or commercial efforts are worth the few dollars it would cost
for spawn and the companies out there do all the research for you for very
little money considering.  I suppose legally the storebought can be expanded
for sale but you might need to check with a lawyer on that.  If you are just
fooling around with a few bags of sawdust-go for it!!  Mike Ziegler


From cina@aimnet.com Fri Feb 10 22:31:58 EST 1995
Article: 1428 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!swrinde!cs.utexas.edu!news.sprintlink.net!aimnet1.aimnet.com!aimnet.aimnet.com!not-for-mail
From: cina@aimnet.com (Chinese Information and Networking Association)
Newsgroups: bionet.immunology,bionet.biology,tropical,bionet.mycology
Subject: SOS, help save the Chinese girl's life
Date: 9 Feb 1995 21:36:25 -0800
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Xref: bigblue.oit.unc.edu bionet.immunology:2929 bionet.mycology:1428



     Call For Help!

Non-infection Necrosis? Infectious necrosis? or
Localized menifestation of an unknown systemic disease?

 ---  A young Chinese girl is slowly being eaten away by
a strange disease.

A young  Chinese girl suffering from a mysterious infection
that causes progressive necrosis of her right forearm and
left hand is arousing great concern in the whole country.
After fighting the disease for nearly four months without
any success, doctors in Beijing are appealing for emergency
help from colleagues arround the world.

The thirteen year old girl, Yang Xiaoxia comes from
a poor farm family in Shangdong province in east China.
The girl has been tortured by the mysterious disease
for 8 months. The disease first began as a black spot
under a finger nail of her right hand suspected by a
local clinic as a stab wound caused by small stick or barb.
A local doctor tried to get something out of the black spot,
but thing got worse. She began to suffer from paronychiais
and the infection soon spread to the whole right forearm
and the mid-finger on her left hand.

After treatments in five different hospitals in provincial
capital Jinan had all failed, the girls parents took
her to Beijing. She has hospitalized in a Beijing military
hospital since early November, but the disease
continued to progress despite all out efforts by the
city's most elite doctors. The doctors have to amputate
one third of her right forearm and three fingers of
her left hand. Although the spreed of the infection
seems a little bit slower, still there was no sign the
infection will stop.

What puzzled the whole medical community is that
from the appearance of the affected surfaces it looks
like an infection, but the infection have
failed to response to any exsiting antibiotics
and hyperbaric oxgen treatment. Bacterior cultures
have always been negtive.

Officals of the city public health bureau have
invited experts to probe into the mystirous disease
and welcomes any suggestions from doctors
around the world.

We will continue to update information on
Yang Xiaoxia's condition.  If you have any
suggestions, please send e-mail to the
following address:

Huangxd@BEPC2.IHEP.AC.CN

Dr. Xiangdong Huang

Institute of Medical Information,
27 Taiping Road,
Beijing 100039,
China.
Tel:86-1-8215603
Fax:86-1-8213044






From MZieg1234@aol.com Fri Feb 10 22:31:59 EST 1995
Article: 1429 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!swrinde!howland.reston.ans.net!news.sprintlink.net!news.clark.net!rahul.net!a2i!olivea!biosci!aol.com!MZieg1234
From: MZieg1234@aol.com
Newsgroups: bionet.mycology
Subject: More on Companion Cultures
Date: 9 Feb 1995 22:07:43 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 47
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 Ralph Arnold suggested I expand on my note of the other day
concerning primordia formation in Grifola frondosa(GF)in
"companion" cultures. The substrate conditions were similar to
those I have used for growth of many other wood-inhabiting fungi.
Grifola was expanded from MEA agar to winter wheat to sawdust
fruiting substrate. 500ml volume of wheat spawn was used per 5lb
bag. Substrate is 94% sawdust (8/2 oak sawdust/maple chips), 5%
organic wheat bran, 1% gypsum. Moisture was to the point of a
few drops when squeezed hard. Each bag also had about 1 Tbs.
blackstrap molasses. Autoclave.... Incubation is in the dark at 72F. 
Experimental bags had additional volume of companion fungus
added at the beginning of  incubation.  Bags were sealed and
agitated to give through-spawn conditions.  One species was added
at equivolume wheat spawn. The other was added as50mls liquid
innoculum.  Incubation was for 40 days, then star shaped
holes(2)were made at the top corners of the bag and all gasses
expelled and the top of the bag tied down per suggestion of 
Annette at Northwest Myc Cons. Temperature was dropped to 60
degrees at 85-95% humidity. I have heard that Asian cultivators
give GF a real cold shock-40's for 24-48 hours but I haven't tried
this .  

Both positive species interact very little with GF, that is when incubated
together the mycelium grow through one another without forming interaction
zones as do some species e.g. trichoderma.  One species that gave negative
results was sulfur shelf. Interaction zones did form here and hard raised
orange plateaus resulted( I had Fall
conditions and might try Summer instead here to mimic sulfur shelf requirement
s next time)! Both species which gave positive results were cool weather
fruiters-one Spring and one Fall. As I noted before both sets of bags gave
primordia of GF only even though control bags grew and fruited nicely.  I
have been trying dual Morel cultures(I know it doesn't make any sense
considering the published lifecycle but what the heck, this is only a weird
hobby)  but find that it overgrows so quickly that companion mycelium get esta
blished only minimally.  I will be adjusting innoculum accordingly in future
tries.

As I said before these results are PRELIMINARY and may not pan out, but given
my singular failings with GF in the past they certainly took me by surprise.
Since there is nothing patentable here (anybody is free to throw two
mushrooms in a bag thank you) I feel OK sharing this much and hope that
anyone can grow any mushroom he or she
pleases for both pleasure and profit and anything I can do to help I will.

 I would be happy to receive any comments on the above. Happy growing to all
who try.
Mike Ziegler from SNOWY Vermont.


From jolivas@CYBERGATE.COM Fri Feb 10 22:32:00 EST 1995
Article: 1430 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!howland.reston.ans.net!news.moneng.mei.com!uwm.edu!biosci!CYBERGATE.COM!jolivas
From: jolivas@CYBERGATE.COM (John L. Olivas)
Newsgroups: bionet.mycology
Subject: Alginate
Date: 10 Feb 1995 10:41:26 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
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Fellow Netters,
        I am looking for a copy of the following article:

                AU: Strullu,-D.G.; Plenchette,-C.
                TI: The entrapment of Glomus sp. in alginate beads and their 
use                         in root inoculum.
                SO: Mycol-Res. Cambridge : Cambridge University Press. Oct   
                      1991. v. 95 (pt.10) p. 1194-1196
                CN: DNAL QK600.M82

        Any assistance or advise would be greatly appriciated.

Regards,

John Olivas
BioScientific, Inc.
3574 W. Escalon Ave.
Fresno, CA. 93711
Ph. 209-269-1152
Fax. 209-439-8639
Email jolivas@cybergate.com



From brianmc@aol.com Mon Feb 13 23:59:58 EST 1995
Article: 1431 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!swrinde!cs.utexas.edu!news.sprintlink.net!uunet!in1.uu.net!newstf01.news.aol.com!newsbf02.news.aol.com!not-for-mail
From: brianmc@aol.com (BrianMc)
Newsgroups: bionet.mycology
Subject: Re: Honey Mushrooms are Tree killers?
Date: 11 Feb 1995 00:03:49 -0500
Organization: America Online, Inc. (1-800-827-6364)
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Tom Volk is the current "Expert of Note" on the topic of Armillaria.  If
he says it's so, I'm certainly not about to disagree.  Armillaria has been
something of a running topic of late, and I've picked up on a few threads
here for inclusion in my e-mail newsletter, MycoInfo.

If the participants don't mind,  I'd like to cross-post this discussion as
well.

Reports from 1992 of "Humoungous Fungus" from upper Michigan, and SW
Washington state, were both species of Armillaria.  It definitely one of
the more ubiquitous fleshy fungi.
----------------------------------------------------------
| Brian McNett                  e-mail: <brianmc@aol.com>
| Editor: MycoInfo         Submissions: <mycoinfo@aol.com> 
|        Promoting Mycology in the Online Community 


From rpeter@nmsu.edu Tue Feb 14 00:00:00 EST 1995
Article: 1432 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!swrinde!howland.reston.ans.net!vixen.cso.uiuc.edu!uwm.edu!caen!newsxfer.itd.umich.edu!jobone!lynx.unm.edu!dns1.NMSU.Edu!bilbo!rpeter
From: rpeter@nmsu.edu (Peter Herman x5495)
Newsgroups: bionet.mycology
Subject: Re: calcium in fungi
Date: 10 Feb 1995 20:07:45 GMT
Organization: New Mexico State University
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In article <9502072052.AA01079@mani.cbs.umn.edu> nora@molbio.cbs.umn.edu writes:
>I would appreciate some help in designing/assessing experiments to  
>determine cytoplasmic calcium levels in Neurospora crassa or another  
>fungus. Specific questions are: 
>
>	(1) Does the esterified fura-2 work in Neurospora for  
>fluorescent measurements of calcium, or what are the drawbacks to its  
>use in fungi? 
>
>	(2) Does the calcium ionophore ionomycin work in Neurospora,  
I have had some luck in using it in the watermold Achlya.  My
problem had to do with keeping the mycelium in place when we exposed
it to the growth regulator we were testing.  Even thin agar didn't
allow quick diffusion of the regulator and gave messy backgrounds.


*   Peter Herman, Dept. of Biology	Phone: +1 505 646 4532    *
*   New Mexico State University		Fax:   +1 505 646 5665    *
*   Box 30001, Dept. 3AF					  *
*   Las Cruces, NM 88003 USA		e-mail rpeter@nmsu.edu    *


From VPGH51A@prodigy.com Tue Feb 14 00:00:02 EST 1995
Article: 1433 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!swiss.ans.net!prodigy.com!usenet
From: VPGH51A@prodigy.com (Jim Berlstein)
Newsgroups: bionet.mycology
Subject: Early Seattle mushrooms
Date: 11 Feb 1995 10:09:45 GMT
Organization: Prodigy Services Company  1-800-PRODIGY
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     While strolling through some local woods and ball fields with my 
fungus hunting budies (Great Danes, of course) I spotted some spring 
entalomas (in grass) and and some Amianita Pantherinas (in woods).  
Apparently winter is coming on sunday, but I was wondering if others have 
seen any extremely early fruitings.  Also, does anyone think that this 
weird weather will bring on morel season early as well? Anyone find any 
verpas yet? Some nice morels appeared last year were the city did some 
digging by the "mailbox rack" in front of my neihbors house.  As he has 
graded his trailer parking area only twenty feet away from there, I'm 
expecting BIG things this year!

Waiting for spring in Seattle

-
  JIM BERLSTEIN  VPGH51A@prodigy.com




From VPGH51A@prodigy.com Tue Feb 14 00:00:04 EST 1995
Article: 1434 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!news-server.ncren.net!news.duke.edu!solaris.cc.vt.edu!swiss.ans.net!prodigy.com!usenet
From: VPGH51A@prodigy.com (Jim Berlstein)
Newsgroups: bionet.mycology
Subject: Re: <ad> GUARANTEED CREDIT REPAIR BY LAW FIRM
Date: 9 Feb 1995 15:14:33 GMT
Organization: Prodigy Services Company  1-800-PRODIGY
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How dare you post this crap in the bionet.mycology section?
You should be ashamed of yourselves.
You will only succeed in convincing people that you are slime.
Credit repair? 
We don't need no stinkin credit repair.





From peburnsx@prairienet.org Tue Feb 14 00:00:06 EST 1995
Article: 1435 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!howland.reston.ans.net!vixen.cso.uiuc.edu!prairienet.org!peburnsx
From: peburnsx@prairienet.org (Pamela E. Burns-Balogh)
Newsgroups: bionet.mycology
Subject: Natural History BBS
Date: 11 Feb 1995 13:06:33 GMT
Organization: University of Illinois at Urbana
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NNTP-Posting-Host: firefly.prairienet.org


Our mycology catalog is now available for downloading off our
direct dial BBS, modem number is 1-217-355-4532, set your your
screen to ANSI, download protocol to ZMODEM, takes about
20 seconds to see the   please wait.... remark. other subject area
catalogs also available.
koeltz Scientific Books
-- 
Pamela Burns-Balogh
Koeltz Scientific Books USA
Champaign, IL 61821


From kasplash@crl.com Tue Feb 14 00:00:08 EST 1995
Article: 1436 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!swrinde!howland.reston.ans.net!nntp.crl.com!crl.crl.com!not-for-mail
From: kasplash@crl.com (Bob Galvan)
Newsgroups: bionet.mycology
Subject: Re: Early Seattle mushrooms
Date: 11 Feb 1995 09:29:14 -0800
Organization: CRL Dialup Internet Access	(415) 705-6060  [login: guest]
Lines: 5
Message-ID: <3his5a$hrl@crl.crl.com>
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NNTP-Posting-Host: crl.com

Here in Marin county, California Amanita pantherina has been common as
cockroaches all January, and fading fast now as Amanita velosa pops up by
every Coast Live Oak.  Lots of A. rubescens too.  Poor year for
chanterelles here, though there have been good reports 50-100 miles south. 



From s9104735@bf.rmit.edu.au Tue Feb 14 00:00:10 EST 1995
Article: 1437 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!rutgers!gatech!newsxfer.itd.umich.edu!zip.eecs.umich.edu!caen!msunews!harbinger.cc.monash.edu.au!aggedor.rmit.EDU.AU!s9104735
From: s9104735@bf.rmit.edu.au (Rosemary Waghorn)
Newsgroups: bionet.mycology
Subject: Info on metal uptake by fungi wanted
Date: 13 Feb 1995 01:39:52 GMT
Organization: Royal Melbourne Institute of Technology, Melbourne, Australia.
Lines: 14
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Summary: metal uptake by fungi
Keywords: help fungi metal
X-Newsreader: NN version 6.5.0 (NOV)

Does anyone know of any information about heavy metal (especially lead) 
uptake of dried preparations of fungi, in heavy metal contaminated soils 
or streams?

Any book or journal references would be appreciated.

Or about anything related.      Thanks

..Rosemary..

--
--------------------------------------------------------------------------
Ladyhawke                        Practise random kindness and sensless acts      
s914735@otto.bf.rmit.oz.au       of beauty.


From qwu@ag.arizona.edu Tue Feb 14 00:00:12 EST 1995
Article: 1438 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!howland.reston.ans.net!news.sprintlink.net!uunet!CS.Arizona.EDU!news.Arizona.EDU!nectria.agmarley.arizona.edu!user
From: qwu@ag.arizona.edu (Qindong Wu)
Newsgroups: bionet.mycology
Subject: phyophthora DNA
Followup-To: bionet.mycology
Date: Mon, 13 Feb 1995 17:07:11 +0100
Organization: University of Arizona
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NNTP-Posting-Host: nectria.agmarley.arizona.edu

A grad student in our department wants to bring samples of phytophthora
into
the states from Mexico, but realizes that live samples will not be
permitted.
Due to the nature of the project he is conducting, it will not be possible
to
extract DNA while in Mexico.  What we have discussed, and what I am hoping
to
find out by making this posting, is whether it is possible to preserve
tissue, grown axenically, in ethanol and bring it back for DNA extraction.
Any advice would be greatly appreciated and can be forwarded to me or
posted here.
Thanks, Kevin McCluskey
mccluske@ag.arizona.edu


From cmody@acs.ucalgary.ca Tue Feb 14 00:00:14 EST 1995
Article: 1439 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!howland.reston.ans.net!news.sprintlink.net!uunet!in1.uu.net!olivea!nntp-hub.barrnet.net!decwrl!tribune.usask.ca!quartz.ucs.ualberta.ca!rover.ucs.ualberta.ca!news.ucalgary.ca!pc12.mid.ucalgary.ca!user
From: cmody@acs.ucalgary.ca (Dr. C. Mody)
Newsgroups: bionet.mycology
Subject: Do I have cell wall fraction of yeast
Date: Mon, 13 Feb 1995 18:13:28 -0700
Organization: MID
Lines: 17
Message-ID: <cmody-1302951813280001@pc12.mid.ucalgary.ca>
NNTP-Posting-Host: pc12.mid.ucalgary.ca

Can anybody tell me how to be resonably sure that I have separated the
cell wall and cytosolic fractions of Cryptococcus neoformans. C.
neoformans was grown in Sabs broth, killed with heat (autoclaved), washed
and about 10^9 organisms placed in a glass bead mill [Bead Beater®]
(volume of organisms 75 ml, beads 100 ml, buffer to fill chamber). Rotated
one minute on, one minute off for 60 minutes on ice.  Disruption was
complete by microscopic exam. A slow spin was done to remove debris.  The
preparation was spum at 13,000 xg for 30 minutes, and the pellet and
supernatant was collected. My reading would indicate that the pellet
should contain the cell wall, the supernatant the cytosol.  How can I be
more sure? Is there a simple assay that can be done to tell that my cell
wall preparation has minimal contamination with cytosolic proteins and
vice versa?

Thanks
Chris Mody
cmody@acs.ucalgary.ca


From hkilpatr@osf1.gmu.edu Tue Feb 14 00:00:16 EST 1995
Article: 1440 of bionet.mycology
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From: hkilpatr@osf1.gmu.edu (HENRY E. KILPATRICK JR.)
Newsgroups: bionet.mycology
Subject: Re: Cooking Mushrooms?
Date: 13 Feb 1995 23:00:03 GMT
Organization: George Mason University, Fairfax, Virginia, USA
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Steven H. Weiss (stweiss@cello.gina.calstate.edu) wrote:
: Does anyone have any suggestions for cookbooks that specialize 
: in cooking wild mushrooms?  Also, are there recipes anywhere 
: out there on the Net?
:  
: Thanks,
: Steve Weiss  

--
The best one I've seen is written by Jack Czarnecki (sp?), who own's 
Joe's Restaurant in Reading, PA.  I think the name of the book is something 
like Joe's Book of Mushroom Cookery.  I have the book at home, so e-mail 
me if you need the exact name.

Buddy Kilpatrick


From staben@seqanal.mi.uky.edu Tue Feb 14 00:00:18 EST 1995
Article: 1441 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!news-feed-1.peachnet.edu!ukma!seqanal.mi.uky.edu!staben
From: staben@seqanal.mi.uky.edu (Chuck Staben)
Newsgroups: bionet.mycology
Subject: G-protein effectors
Date: 14 Feb 1995 02:37:57 GMT
Organization: University of Kentucky, Dept. of Math Sciences
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I wonder whether contributors to this forum might know which G-protein
effectors are effective in either activating or inactivating the
G-alpha signal transduction pathway in intact fungi.  Although there are
a million inhibitors used with G-proteins,
 and a lot of genetic work on S. cerevisiae, it is
not clear to me how best to find this specific info.
P.S.  This is not a Neurospora-related question, but I have not deserted
the one true organism--as readers of previous queries have queried


From gyetter@PANIX.COM Tue Feb 14 00:00:20 EST 1995
Article: 1442 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!howland.reston.ans.net!news.sprintlink.net!uunet!olivea!biosci!PANIX.COM!gyetter
From: gyetter@PANIX.COM (Gene Yetter)
Newsgroups: bionet.mycology
Subject: The Mycologia Index, Vols. 1-58
Date: 13 Feb 1995 20:24:49 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
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Regular mycology newsgroup readers may recall the announcement I posted in
December 1994 in which I sought volunteers to help transcribe the
Mycologia Index, Vols. 1-58, to disk so that it could be made available
online at the Telnet site, fungi.ars-grin.gov.  I will scan the 1100-page
Index and farm out to volunteers the task of cleaning-up and proofing of
the scanner output. 

The project is still on track.  Of the entire index, the first 
section, Fungus Taxa, through p. 514, has been scanned, broken up 
into blocks of pages and assigned to volunteers.  About half of 
that number of pages have been cleaned up and e-mailed back to me.  We're 
about a quarter of the way home.

The individuals who have returned cleaned-up scanner output to 
date include: Felix (fgrun@mail.med.cornell.edu>), Kathie Hodge 
(Cornell University), Fred Rhoades (Bellingham, WA), Leo J. Van 
Griensven (The Netherlands), Brian McNett (Bremerton, WA), Richard 
Woodside (Canada) and Eugene Varney, Ph.D. (Rutgers University).  
Leo is the runaway leader with 120 pages returned.  Quite an 
international cooperative effort!

Several individuals have agreed to assist who have not yet been 
sent scanner output.  This situation will be corrected as soon as 
I can devote more time to scanning.  Additional volunteers will be 
needed as we swing into the Host Index and Subject Index sections.

Many thanks to those who have participated or are still working on 
their sections.  Please communicate with me if you missed the earlier 
posting and would like to know more.

Gene Yetter
New Jersey Myc. Assoc. & New York Myc. Society

Reply to: gyetter@panix.com





From gen066@nof.abdn.ac.uk Tue Feb 14 09:20:11 EST 1995
Article: 1443 of bionet.mycology
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From: gen066@nof.abdn.ac.uk (b.morris)
Newsgroups: bionet.mycology
Subject: Re: calcium in fungi
Date: 14 Feb 1995 13:23:32 GMT
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<9502072052.AA01079@mani.cbs.umn.edu> nora@molbio.cbs.umn.edu writes: 
: >I would appreciate some help in designing/assessing experiments to 
: >determine cytoplasmic calcium levels in Neurospora crassa or another 
: >fungus.  Specific questions are: : > 
: > (1) Does the esterified fura-2 work in Neurospora for :..............



I can't help with the specifics but you may find some food for thought in the
following papers :-

Knight, H. et al. (1993) 
Confocal microscopy of living fungal hyphae microinjected with Ca2+-sensitive 
fluorescent dyes. Mycological Research 97(12), 1505-1515

Read, N.D. et al. (1992)
Imaging and measurement of cytosolic free calcium in plant and fungal cells.
Journal of Microscopy Vol 166, Part 1 April 1992, 57-86

Mike Morris @ Aberdeen, Scotland


From stweiss@cello.gina.calstate.edu Fri Feb 17 22:24:38 EST 1995
Article: 1444 of bionet.mycology
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From: stweiss@cello.gina.calstate.edu (Steven H. Weiss)
Newsgroups: bionet.mycology
Subject: Cooking Mushrooms?
Date: 13 Feb 1995 07:36:28 -0800
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Does anyone have any suggestions for cookbooks that specialize 
in cooking wild mushrooms?  Also, are there recipes anywhere 
out there on the Net?
 
Thanks,
Steve Weiss  


From kasplash@crl.com Fri Feb 17 22:24:40 EST 1995
Article: 1445 of bionet.mycology
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From: kasplash@crl.com (Bob Galvan)
Newsgroups: bionet.mycology
Subject: Re: Cooking Mushrooms?
Date: 13 Feb 1995 19:54:39 -0800
Organization: CRL Dialup Internet Access	(415) 705-6060  [login: guest]
Lines: 20
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NNTP-Posting-Host: crl2.crl.com

I know of two good books on wild mushroom cookery.   

1> Wild About Mushrooms
   Cook book of the San Francisco Mycological Society
   I forget the author, (if even listed) and it is out of print,
   but I found it in my local library.     

2> A Passion for Mushrooms
   kinda guessing on the author's name here - Paolo Ciccini
   he has several other books out, Passion for Pasta, Passion for etc...

   This book is loaded with beatiful, inspiring photos of finished 
   mushroom dishes in addition to photos (some obviously staged) of wild 
   mushrooms growing in the forest.   Highly recommended.  Recipes too.
   European perspective.   Seasons will not match ours at all.


Bob Galvan
kasplash@crl.com



From pb019@CSC.ALBANY.EDU Fri Feb 17 22:24:42 EST 1995
Article: 1446 of bionet.mycology
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From: pb019@CSC.ALBANY.EDU (Partha Banerjee)
Newsgroups: bionet.mycology
Subject: (none)
Date: 14 Feb 1995 08:49:13 -0800
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It would be very nice if we could start a mycology job bulletin board on
our newsgroup.  It would also be worthwhile if we could start a column on
why mycology jobs are so scarce and how we can create new jobs in mycology.
The potential role of MSA and other like organizations and our schools and
faculties in job-related matters should also be discussed.





From stewart@bud.peinet.pe.ca Fri Feb 17 22:24:43 EST 1995
Article: 1447 of bionet.mycology
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From: stewart@bud.peinet.pe.ca (Paul Stewart)
Newsgroups: bionet.mycology
Subject: re: metal uptake by fungi
Date: 13 Feb 1995 08:47:21 -0400
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Lines: 32
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Dear Rosemary,

	In response to your request for references on heavy metal uptake,
first assume that the average water content of a fresh mushroom is 85%.  
This means that dried preparations will have about 10X the amount of 
contaminant reported for fresh.  However, most tox. data on heavy metals 
is expressed as %dry matter.  Here are a few references that may be helpful:
(refs marked by a "*" are in my files...if you can't find a copy I can 
mail you one, if you don't mind the wait!)

* Yasui, A., Tsutsumi, C., Takasaki, M., and T. Mori, 1988.  (text in 
Japanese, abstract and tables in english)  Absorption of elements from 
heavy-metals-containing culture media by oyster mushroom (P. ostreatus)
NIPPON SHOKUHIN KOGYO GAKKAISHI (J.Jap.Soc.FoodSci.Tech)
35(3):160-165. (Cu, Cd, Pb, Ge...only Cd 
was uptaken, higher in cap than stem)(Nat Food Res Inst, Min Agri, For, & 
Fish, 2-1-2, Kannondai, Tsukuba-shi, Ibaraki, 305, Japan)

  Strmiskova, G., Strimiska, F., and J. Dubravicky, 1992. Mineral 
composition of oyster mushroom, NAHRUNG: FOOD 36(2):210-212. (G. Strm. @ 
Slovak Tech Univ, Fac Chem Technol, Radlinskeho Ul 9, CS-81237 
Bratislava, Czechoslovakia)

 Mandic, ML, Grgic, J, Grgic, Z., and M. Seruga, 1992. The natural levels 
of aluminum, cadmium, and lead in wildlife mushrooms in Eastern Croatia, 
DEUTSCHE LEBENSMITTEL - RUNDSCHAU 88(3):76-77 (ML Mandic, Univ. Osijek, 
Fac Food Technol, YU-54000, Osijek, Yugoslavia

Hope these are of some help. There are probably more recent ones (ie-from 
countries that still exist!); try looking in Food Science and Technology 
Abstracts, or contact Leatherhead in England for a complete search 
(they're pricy though thorough!)...Paul From The Great White Northeast. 


From wharrison@aol.com Fri Feb 17 22:24:44 EST 1995
Article: 1448 of bionet.mycology
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From: wharrison@aol.com (WHARRISON)
Newsgroups: bionet.mycology
Subject: Re: Growing mushrooms on Internet
Date: 11 Feb 1995 17:46:12 -0500
Organization: America Online, Inc. (1-800-827-6364)
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Reply-To: wharrison@aol.com (WHARRISON)
NNTP-Posting-Host: newsbf02.mail.aol.com

You can also subscribe to the newsletter of the Colorado Mycological
Society through the internet.  It's free, and chuck full of interesting
articles, if I do say so myself.  I'm the editor.


   _____________________   
  |  ~        ^         |  
  |      ^   /_\   ^  ~ |  
  |     /_\ /   \ / \   |  
  | /\ /   \  ^  \ __\/\|  
  |/  /     \/|\  \   \ |  
  |  / ^     /|\  ^    \|  
  | / /|\  ^  |  /|\  ^ |  
  |/  /|\ /|\    /|\ /|\|  
  |    |   |      |   | |  
  |_____________________|  
       Wayne Harrison
       Pine, Colorado
     elevation 8,500 ft.
      (way up there)


From gren@gram.demon.co.uk Fri Feb 17 22:24:46 EST 1995
Article: 1449 of bionet.mycology
Newsgroups: bionet.mycology
Path: bigblue.oit.unc.edu!concert!rutgers!gatech!howland.reston.ans.net!swrinde!cs.utexas.edu!news.sprintlink.net!peernews.demon.co.uk!demon!gram.demon.co.uk!user
From: gren@gram.demon.co.uk (Gareth Renowden)
Subject: Re: Cooking Mushrooms?
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References: <3hnu9s$jvp@cello.gina.calstate.edu> <3hoo9j$666@portal.gmu.edu> <3hp9hv$5i7@crl2.crl.com>
Date: Tue, 14 Feb 1995 16:07:24 GMT
Lines: 18

In article <3hp9hv$5i7@crl2.crl.com>, kasplash@crl.com (Bob Galvan) wrote:


> 2> A Passion for Mushrooms
>    kinda guessing on the author's name here - Paolo Ciccini
>    he has several other books out, Passion for Pasta, Passion for etc...

He's called Antonio Carluccio. And yes, it is very good.

Another good one - over here, anyway - is The Mushroom Feast by Jane
Grigson, a very famous UK cookery writer.

-- 
Gareth Renowden                      l
Publishing & marketing consultant    l  "I don't know what I want,
Voice +44 (0)181 241 4852            l     but I want it NOW!"
Fax +44 (0)181 943 4294              l    Sir Henry Rawlinson
e-mail gren@gram.demon.co.uk         l     (c) Viv Stanshall


From bradbury@ACS.UCALGARY.CA Fri Feb 17 22:24:47 EST 1995
Article: 1450 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!news-server.ncren.net!news.duke.edu!godot.cc.duq.edu!newsfeed.pitt.edu!gatech!howland.reston.ans.net!spool.mu.edu!olivea!biosci!ACS.UCALGARY.CA!bradbury
From: bradbury@ACS.UCALGARY.CA
Newsgroups: bionet.mycology
Subject: RAPD primers for ecto's
Date: 13 Feb 1995 13:32:46 -0800
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Can anyone advise me as to which RAPD primers maybe useful for amplifying
fungal DNA from colonized ectomycorrhizal root tips (Lodgepole pine)?
Any advice will be greatly appreciated.  Thanks.


From schatzr@mail.firn.edu Fri Feb 17 22:24:48 EST 1995
Article: 1451 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!howland.reston.ans.net!news.sprintlink.net!news.packet.net!usenet
From: 204.96.208.11 (Bob Schatzow)
Newsgroups: bionet.mycology
Subject: Rare Fungi
Date: 15 Feb 1995 15:51:42 GMT
Organization: 204.96.208.1
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I work at Hillsborough Community College and a professor here is very ill with a rare fungal infection.

The doctor has diagnosed Trichosporans Polulans.  We have found Sporothrix Schenkii which has similar symptoms.

If anyone can help us with any information on these fungi it would be greatly appreciated.  Information may help save this professors life.

Please send any information to :
Bob Schatzow 
D. P. Lab Supervisor
Ybor campus
Hillsborough Community College
P.O. Box 5096
Tampa, Florida 33675-5096
schatzr@mail.firn.edu
Phone: (813)253-7616
Fax:(813)253-7610

Thanks for your help
Bob


From ninja@cml.com Fri Feb 17 22:24:50 EST 1995
Article: 1452 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!news-server.ncren.net!news.duke.edu!agate!howland.reston.ans.net!news.cac.psu.edu!news.pop.psu.edu!hudson.lm.com!newsfeed.pitt.edu!uunet!uunet.ca!uunet.ca!news.compulink.com!ninja
From: ninja@cml.com (Joe Sommer)
Newsgroups: bionet.mycology
Subject: Mediums for Pleurotus growth
Date: 12 Feb 1995 04:29:56 GMT
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Hello, I am going to conduct an experiment that deals with growing 
Pleurotus ostreatus on different types of substrates ie, cellulose, 
plastic, plywood, tripple mix soil, and so on.  I want to see which 
medium provides the greatest yield measured by net dry/wet weight measures.

Any suggestions would be appreciated in terms of the methodology and 
previous success on the above mentioned substrates.

Joe

 ---------------------------------------------------------------------
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From mspear@telerama.lm.com Fri Feb 17 22:24:51 EST 1995
Article: 1453 of bionet.mycology
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From: mspear@telerama.lm.com (mspear)
Newsgroups: bionet.mycology
Subject: Re: (none)
Date: 14 Feb 1995 18:13:55 -0500
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Partha Banerjee (pb019@CSC.ALBANY.EDU) wrote:
> It would be very nice if we could start a mycology job bulletin board on
> our newsgroup.  It would also be worthwhile if we could start a column on
> why mycology jobs are so scarce and how we can create new jobs in mycology.
> The potential role of MSA and other like organizations and our schools and
> faculties in job-related matters should also be discussed.




As an employer I agree.  We've had trouble recruiting people,
especially at the Bachelor's and Master's level.  Although we are not
currently seeking new co-workers it would be nice to have a resource
like this.

    ---- Mark


From todell@u.washington.edu Fri Feb 17 22:24:53 EST 1995
Article: 1454 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!hearst.acc.Virginia.EDU!portal.gmu.edu!europa.eng.gtefsd.com!newsxfer.itd.umich.edu!srvr1.flint.umich.edu!gmi!msunews!uwm.edu!news.moneng.mei.com!howland.reston.ans.net!news.sprintlink.net!uunet!in1.uu.net!nntp.cac.washington.edu!homer22.u.washington.edu!todell
From: Thomas O'Dell <todell@u.washington.edu>
Newsgroups: bionet.mycology
Subject: Re: RAPD primers for ecto's
Date: Wed, 15 Feb 1995 09:52:28 -0800
Organization: University of Washington
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The problem as I see it is competition from plant DNA in the roots. You 
can amplify specific fungal target sequences from roots (ITS, IGS, etc.) 
in preference to plant DNA, but with arbitrary priming I dont see how 
you'd know if a particular band is of plant or fungal origin (unless you 
want to do southern blotting or some such). If you're trying to identify 
strains of fungi an alternative approach is to sequence some variable 
region such as the ITS1- ITS2 rDNA.


From mycochef@aol.com Fri Feb 17 22:24:54 EST 1995
Article: 1455 of bionet.mycology
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From: mycochef@aol.com (MYCOCHEF)
Newsgroups: bionet.mycology
Subject: Re: Cooking Mushrooms?
Date: 14 Feb 1995 23:57:05 -0500
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yes, i know a bit about that subject
 e-mail me.

mycochef


From wharrison@aol.com Fri Feb 17 22:24:55 EST 1995
Article: 1456 of bionet.mycology
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From: wharrison@aol.com (WHARRISON)
Newsgroups: bionet.mycology
Subject: Re: Cooking Mushrooms?
Date: 15 Feb 1995 13:17:42 -0500
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The best wild mushroom cookbook I've come across is Hope's Mushroom
Cookbook by Hope Miller.  She is the wife of Orson Miller and if you don't
know who he is you don't know wild mushrooms.  It is published by Mad
River Press inc, 141 Carter Lane, Eureka California 95503.  I think it is
the mushroom cookbook by which others will be judged.
As for recipies on the net, subscribe to some of the myco info services
available...  Some of the online mushroom newsletters, like mine, also
publish recipies...


 ___________________________
|   _____________________   |
|  |  ~        ^         |  |
|  |      ^   /_\   ^  ~ |  |
|  |     /_\ /   \ / \   |  |
|  | /\ /   \     \ __\/\|  |
|  |/  /     \     \   \ |  |
|  |  /       (^)       \|  |
|  | / (^)     |  (^)    |  |
|  |/   |  (^)     |  (^)|  |
|  |        |          | |  |
|  |_____________________|  |
|     S  P  O  R  E  S      |
|       A  F  I  E  L  D   |
|            the            |
|         newsletter        |
|          of the           |]---
|         Colorado          |    \
|        Mycological        |     \
|          Society          |     /
|        via Internet       |    /
|     <sporescms@aol.com>   |    \
 ___________________________     _\__
|  |  |  |  |  |  |  |  |  |    ||  ||
 -- -- -- -- -- -- -- -- --     ||  ||
|  |  |  |  |  |  |  |  |  |     ----
 -- -- -- -- -- -- -- -- --
   Wayne Harrison, Editor
       Pine, Colorado
    elevation 8,500 feet
       (way up there)


From wharrison@aol.com Fri Feb 17 22:24:57 EST 1995
Article: 1457 of bionet.mycology
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From: wharrison@aol.com (WHARRISON)
Newsgroups: bionet.mycology
Subject: Re: Mediums for Pleurotus growth
Date: 15 Feb 1995 13:19:15 -0500
Organization: America Online, Inc. (1-800-827-6364)
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Get a copy of GROWING GOURMET 7 MEDICINAL MUSHROOMS by Paul Stamets.  It
gives complete info on substrates for Pleaurotus and other mushrooms.


 ___________________________
|   _____________________   |
|  |  ~        ^         |  |
|  |      ^   /_\   ^  ~ |  |
|  |     /_\ /   \ / \   |  |
|  | /\ /   \     \ __\/\|  |
|  |/  /     \     \   \ |  |
|  |  /       (^)       \|  |
|  | / (^)     |  (^)    |  |
|  |/   |  (^)     |  (^)|  |
|  |        |          | |  |
|  |_____________________|  |
|     S  P  O  R  E  S      |
|       A  F  I  E  L  D   |
|            the            |
|         newsletter        |
|          of the           |]---
|         Colorado          |    \
|        Mycological        |     \
|          Society          |     /
|        via Internet       |    /
|     <sporescms@aol.com>   |    \
 ___________________________     _\__
|  |  |  |  |  |  |  |  |  |    ||  ||
 -- -- -- -- -- -- -- -- --     ||  ||
|  |  |  |  |  |  |  |  |  |     ----
 -- -- -- -- -- -- -- -- --
   Wayne Harrison, Editor
       Pine, Colorado
    elevation 8,500 feet
       (way up there)


From dje0282@SUMMA.TAMU.EDU Fri Feb 17 22:24:58 EST 1995
Article: 1458 of bionet.mycology
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From: dje0282@SUMMA.TAMU.EDU (Dan Ebbole)
Newsgroups: bionet.mycology
Subject: the one true organism?
Date: 14 Feb 1995 06:53:04 -0800
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Chuck Staben wrote: 

"P.S.  This is not a Neurospora-related question, but I have not deserted
the one true organism--as readers of previous queries have queried"

Chuck, while I realize this is humor, there are others who actually could
be offended by the "snobbishness" of the comment.  Neurospora has some
wonderful advantages and some disadvantages relative to other fungal
systems for examining various aspects of fungal biology.  I just want to
make sure that everyone understands that Neurosporologists generally have
great respect for other fungal systems.  I know Chuck feels the same way
and I'd hate for his comment to be misunderstood.  It is most distasteful
to send a message about politically correct statements regarding fungi-but
here it is.
------------------------
Dr. Daniel Ebbole
dje0282@summa.tamu.edu
Dept. of Plant Pathology and Microbiology
Texas A&M University
College Station, TX 77843-2132



From dje0282@SUMMA.TAMU.EDU Fri Feb 17 22:25:00 EST 1995
Article: 1459 of bionet.mycology
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From: dje0282@SUMMA.TAMU.EDU (Dan Ebbole)
Newsgroups: bionet.mycology
Subject: Calcium measurement in fungi
Date: 16 Feb 1995 14:10:12 -0800
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Someone asked about measuring Ca2+ levels in fungi.  This reference would
be of interest.

Pitt, D. and J.C. Barnes. 1993.  Calcium homeostasis, signalling and
protein phosphorylation during calcium-induced conidiation in Penicillium
notatum.  J. Gen. Microbiol. 139:3053-3063.  

------------------------
Dr. Daniel Ebbole
dje0282@summa.tamu.edu
Dept. of Plant Pathology and Microbiology
Texas A&M University
College Station, TX 77843-2132



From CXJ06603@NIFTYSERVE.OR.JP Fri Feb 17 22:25:01 EST 1995
Article: 1460 of bionet.mycology
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From: CXJ06603@NIFTYSERVE.OR.JP ("CXJ06603@niftyse")
Newsgroups: bionet.mycology
Subject: Re: Hyphomycetes
Date: 16 Feb 1995 10:07:14 -0800
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You can find bibliographical informations in some books or series: Dictionary 
of the Fungi, Genera of Hyphomycetes, Bibliography of Systematic Mycology and 
Index of Fungi.

Gyrothrix Corda and Circinotrichum Nees

  Pirozynsky, K. A.: CMI Mycol. Pap. 84: 3-27, 1962.
  Pirozynsky, K. A. & S. D. Patil: Some setose Hyphomycetes of leaf litter
     in south India. Can. J. Bot. 48: 567-581, 1970.
  Pirozynsky, K. A. & C. S. Hodges: New hyphomycetes from South Carolina. 
     Can. J. Bot. 51: 157-173, 1973.
  Cunningham, J. L.: A new Gyrothrix in culture and a key to species. 
     Mycologia 66: 122-129, 1974.

Vermiculariopsiella Bender

  Bender: Mycologia 24-42, 1932
  Nag Raj, T. R.: Vermiculariopsiella Bender, an earlier name of Oramasia 
     Urries. Mycotaxon 18: 159-163, 1983.

Yasuhisa Tsurumi
CXJ06603@niftyserve.or.jp


From Louis.bernier@sbf.ulaval.ca Fri Feb 17 22:25:03 EST 1995
Article: 1461 of bionet.mycology
Newsgroups: bionet.mycology
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From: Louis.bernier@sbf.ulaval.ca (Louis Bernier)
Subject: infection of fungi by protozoa-thanks
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My previous query for information on how to rid fungal cultures of protozoa
elicited an unexpectedly high number and diversity of answers from members
of the mycology and forest pathology electronic networks. My thanks to all
of you who contributed information. I will post the results we have
obtained following application of the various protocols. Stay tuned.

Louis Bernier


From KIRTIKARA@AESOP.RUTGERS.EDU Fri Feb 17 22:25:04 EST 1995
Article: 1462 of bionet.mycology
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From: KIRTIKARA@AESOP.RUTGERS.EDU
Newsgroups: bionet.mycology
Subject: test
Date: 17 Feb 1995 12:30:11 -0800
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test



From koolaid@vircom.com Fri Feb 17 22:25:06 EST 1995
Article: 1463 of bionet.mycology
Newsgroups: bionet.mycology
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From: koolaid@vircom.com (Jeff Haynes)
Subject: My bro helped with Highlander series!
Organization: The GameMaster, Montreal, Canada.
Date: Fri, 17 Feb 1995 02:47:39 GMT
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Hi!

My brother was a Dolly Grip in the production of the HighLander series.
Anyone that post to this newsgroup or read it work on it too?

Get back to me via E-Mail please.

THanks.

Ryan



From seancoo@wam.umd.edu Sun Feb 19 12:13:04 EST 1995
Article: 1464 of bionet.mycology
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From: sean kevin cooney <seancoo@wam.umd.edu>
Newsgroups: bionet.mycology
Subject: How to clone mushrooms?
Date: Thu, 16 Feb 1995 11:07:19 -0500
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	I was hoping someone out there could explain to me how to clone 
a mushroom or point me to some literature that would explain the process 
to me.  And furthermore is the process species specific.  I'm a poor 
undergraduate who loves shiitake mushrooms and other wild species, but can't 
really afford them or get out enough to collect them as often as I'd 
like.  I have access to a lab and autoclave (etc.), in which I work, so I 
have everything I need (I think) at my finger-tips but the know-how.  Any 
help would be greatly appreciated!!
				thanks,
					Sean (seancoo@wam.umd.edu)




  

















From s_hope@cix.compulink.co.uk Sun Feb 19 12:13:05 EST 1995
Article: 1465 of bionet.mycology
Newsgroups: bionet.mycology
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From: s_hope@cix.compulink.co.uk ("Simon Hope Incode")
Subject: Re: Rare Fungi
Message-ID: <D468vz.Ep6@cix.compulink.co.uk>
Organization: Compulink Information eXchange
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Date: Sat, 18 Feb 1995 01:16:47 GMT
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You have mail on S. Schenkii. 

Simon Hope.


From VPGH51A@prodigy.com Sun Feb 19 12:13:08 EST 1995
Article: 1466 of bionet.mycology
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From: VPGH51A@prodigy.com (Jim Berlstein)
Newsgroups: bionet.mycology
Subject: Re: My bro helped with Highlander series!
Date: 18 Feb 1995 07:55:27 GMT
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Is it true that Duncan Macloud has a real taste for boletus edulis?
And His friend Ritche prefers Cantherellus Subalbidus?
I here rumors that Joe, the Watcher who owns the bar indulges in the 
Psilocybes a bit too freely.
And Macloud's old Girlfriend, Tess, died from eating Aminita phalloides, 
right?
Isn't it amazing how much mycology there is in this sci-fi TV show!

Or, perhaps I'm mistaken and there is no reason why a TV show should 
come up in the mycology topic.

Could be worse, could be Lawyers advertising Credit Repair.
What can you do?

Is celluloid a good medium for growing pleurotus?

Silly Jim

-
  JIM BERLSTEIN  VPGH51A@prodigy.com





From pub907@IDPTV.IDBSU.EDU Sun Feb 19 12:13:09 EST 1995
Article: 1467 of bionet.mycology
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From: pub907@IDPTV.IDBSU.EDU (Warren Rekow)
Newsgroups: bionet.mycology
Subject: Re: How to clone mushrooms?
Date: 17 Feb 1995 14:33:23 -0800
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On 16 Feb 1995 Sean Cooney wrote:
>        I was hoping someone out there could explain to me how to clone 
>a mushroom or point me to some literature that would explain the process
>to me.  ....

Sean, a lab and autoclave may provide you with the the essentials for doing
tissue culture or spore germinations on agar, but making the transition
from petri dishes to fruited mushrooms is another matter.  Your first task
should be buying a copy of and studying the book "The Mushroom Cultivator"
authored by Paul Stamets & J.S. Chilton.  Paul can be reached online at the
following address:  mycomedia@aol.com.  Be sure to also get the
Fungi Perfecti catalog from him.

You may also want to subscribe to the FUNGUS mail list, which is for people
interested in mushroom cultivation.  The moderator, Ralph Arnold, can be
contacted at:  rarnold@teleport.com.

-- 
Warren Rekow (pub907@idptv.idbsu.edu)
HC 79, Box 104, Melba  ID  83641
Phone or fax: 208-896-4908


From JHARRIS@LABA.TDH.TEXAS.GOV Sun Feb 19 12:13:10 EST 1995
Article: 1468 of bionet.mycology
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From: JHARRIS@LABA.TDH.TEXAS.GOV ("Jim Harris")
Newsgroups: bionet.mycology
Subject: teleconference
Date: 17 Feb 1995 14:40:39 -0800
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Medical Mycology Teleconference ("Old and New Approaches to Medical 
Mycology") directed at clinical laboratory scientists on the Teleconference 
Network of Texas is scheduled for March 28, 1995 at 1:30-2:30 CST.  
Contact the Teleconference Network at 1-800-982-8868 and ask for William 
Salada to get more information
jharris@LABA.tdh.texas.gov


From NREAD@SRV0.BIO.EDINBURGH.AC.UK Sun Feb 19 12:13:12 EST 1995
Article: 1469 of bionet.mycology
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From: NREAD@SRV0.BIO.EDINBURGH.AC.UK (Nick Read)
Newsgroups: bionet.mycology
Subject: re: calcium in fungi
Date: 17 Feb 1995 11:31:41 -0800
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In our experience, Fura-2 cannot be ester-loaded into 
Neurospora hyphae.  If it is microinjected as its free 
acid, it is rapidly sequestered within organelles 
making measurements of cytosolic free calcium 
impossible.  We have documented the problems of using 
calcium dyes with fungi in some detail 
in Knight et al. (1993).  Mycol. Res. 97: 1505 and in 
Read et al. (1992).  J. Microsc. 166: 57.

We haven't used ionomycin but have had success with 
bromo-A23187 (see Knight et al., 1993).

Nick Read 


From stewart@bud.peinet.pe.ca Sun Feb 19 12:13:13 EST 1995
Article: 1470 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!howland.reston.ans.net!torn!news.unb.ca!peinet.pe.ca!peinet.pe.ca!not-for-mail
From: stewart@bud.peinet.pe.ca (Paul Stewart)
Newsgroups: bionet.mycology
Subject: Re: microwave
Date: 18 Feb 1995 13:18:45 -0400
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To the person attempting microwave media sterilization:

I asked for info on this on this newsgroup a couple of months ago, and 
got a wide range of replies; everything from, "forget it, it won't work"
to, "our lab has never had contamination in years wih our microwaved 
instruments".  The general consensus seems to be that it is chancy at 
best. I have had good luck with liquid media by nuking for 30 minutes on 
High.  You need to have lots of headroom in the bottle, since it will 
boil over otherwise. I put a couple of coffee mugs full of water and that 
reduces the loss of water from the media. You will still lose about 
10-20%, so make the media up with extra water to allow for this. 
	My guess is you should presoak the grain, straw or woodchips, and 
include a fair amount of water when you nuke it. I had to nuke only three 
bottles of straw/grain at a time for about an hour. No contamination yet, 
but I feel microwave works best on small volumes of liquid, where 
hot/cold spots matter less due to mixing.
	Maybe your inquiry will spark discussion on this. I feel the 
topic is far from having reached a consensus. Wadda ya say experts?
......Paul from the Great White Northeast.


From puchalsk@k12.oit.umass.edu Sun Feb 19 12:13:14 EST 1995
Article: 1471 of bionet.mycology
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From: puchalsk@k12.oit.umass.edu (Joshua Puchalski (GCC/TEME))
Newsgroups: bionet.mycology
Subject: microwave
Date: 18 Feb 1995 16:19:17 GMT
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I am interested in experimenting with microwave steralization. I have a few
questions. Using a 700 watt microwave, how many quart sized jars would I do
at once? Second, how long should I cook them for? Should the grain be
precooked, or should I add dry grain and water to the jars, and put them
directly in the microwave oven? Also most importantly, what should I cover
the jars with?

Thank you for any help you may give.
                                                     Joshua Puchalski



From dje0282@SUMMA.TAMU.EDU Sun Feb 19 12:13:15 EST 1995
Article: 1472 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!usenet.ins.cwru.edu!howland.reston.ans.net!spool.mu.edu!olivea!biosci!SUMMA.TAMU.EDU!dje0282
From: dje0282@SUMMA.TAMU.EDU (Dan Ebbole)
Newsgroups: bionet.mycology
Subject: For Neurosporologists only
Date: 18 Feb 1995 09:12:56 -0800
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There are few mutants in the FGSC that are specifically blocked in
conidiation. Nine alleles of fluffy, and one each of fluffyoid, acon-2 and
acon-3.  David Perkins has suggested that aconidials are very frequently
observed spontaneously but that people do not routinely save these
aconidial strains.  It would really help us if anyone finding an aconidial
strain that appears to grow at near wild type rate would pass the strain
along to me.  Finding such mutants by screening mutagenized conidia
certainly can be done. A screen of 3500 survivors of EMS treatment yielded
1 csp strain (Selitrennikoff, Nelson and Siegel. 1974. Genetics
78:679-690). Matsuyama, Nelson, and Seigel. 1974. Dev. Biol.41:278-287
reported that in screening 1 x 10^5 colonies they found 10^3 aconidials of
which a total of 2 (acon-2 and acon-3) were found to be unaffected in
vegetative growth (although acon-3 is female sterile).  

Please, if you find aconidials with good growth characteristics, please
characterize them yourself or send them along. Thanks.
------------------------
Dr. Daniel Ebbole
dje0282@summa.tamu.edu
Dept. of Plant Pathology and Microbiology
Texas A&M University
College Station, TX 77843-2132



From intron@eskimo.com Sun Feb 19 12:13:16 EST 1995
Article: 1473 of bionet.mycology
Newsgroups: bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!usenet.ins.cwru.edu!howland.reston.ans.net!news.moneng.mei.com!uwm.edu!news.alpha.net!mvb.saic.com!eskimo!tia1.eskimo.com!user
From: intron@eskimo.com (Dr. Karl Goldkamp)
Subject: doing research on Ganoderma lucidium immunology paper
X-Nntp-Posting-Host: tia1.eskimo.com
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  Does any one have any information on Ganoderma alias Lingzhi, or
Reishi.  I'm doning a Immunology paper  on it and have done medline
search.  Im a student at a Naturopathic Medical school here in Seattle. 
Though I know ganoderma is used for cancer in Japan I need to find studies
that bring a western approach to it.  So far LZ-8 appears to be a isolated
polysacchride of interest.  Any timps or leads or greatly appreciated.

E-mail: intron@eskimo.com


From jvpotter@aol.com Sun Feb 19 12:13:18 EST 1995
Article: 1474 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!bloom-beacon.mit.edu!spool.mu.edu!howland.reston.ans.net!swiss.ans.net!newstf01.news.aol.com!newsbf02.news.aol.com!not-for-mail
From: jvpotter@aol.com (JVPotter)
Newsgroups: bionet.mycology
Subject: Re: More on Companion Cultures
Date: 18 Feb 1995 17:37:50 -0500
Organization: America Online, Inc. (1-800-827-6364)
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I was suprised to find ajar of my morel sclerotia producing small
sporocarps after a few months of incubation in darkness . Interestingly,
the jar was contaminated with Rhizopus and Trichoderma . Subsequent
attempts to repeat the success have failed but the companion species may
well have contributed in some way. I would be interested in others doing
additional trials using what would otherwise be considered contaminants.

Bruce Potter
Willits,CA


From harlock999@aol.com Mon Feb 20 22:56:26 EST 1995
Article: 1475 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!willis.cis.uab.edu!ddsw1!godot.cc.duq.edu!news.duke.edu!solaris.cc.vt.edu!news.alpha.net!uwm.edu!cs.utexas.edu!howland.reston.ans.net!pipex!oleane!jussieu.fr!fdn.fr!uunet!in1.uu.net!newstf01.news.aol.com!newsbf02.news.aol.com!not-for-mail
From: harlock999@aol.com (Harlock999)
Newsgroups: bionet.mycology
Subject: Boletus info requested
Date: 18 Feb 1995 20:08:32 -0500
Organization: America Online, Inc. (1-800-827-6364)
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I am trying to find any information regarding Boletus edulis.  This
includes possible innoculum sources, tips on culture, etc. etc. etc.

Any information regarding this would be greatly appreciated.
Harlock999@aol.com


From ndpm@aol.com Mon Feb 20 22:56:28 EST 1995
Article: 1476 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!howland.reston.ans.net!news.sprintlink.net!uunet!newstf01.news.aol.com!newsbf02.news.aol.com!not-for-mail
From: ndpm@aol.com (NDPM)
Newsgroups: bionet.mycology
Subject: Re: microwave
Date: 19 Feb 1995 13:25:35 -0500
Organization: America Online, Inc. (1-800-827-6364)
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NNTP-Posting-Host: newsbf02.mail.aol.com

You can do one jar at a time.
It needs to be wet.
You will nedd at least a 10 minute treatment, probably more.
This method is not effecient unless you are extremely small scale.

send SASE to Phil McIntosh, 7205 glowworm Cr. Austin TX for a reprint of a
paper I wrote on the subject.  Good luck!


From bjorn@kjemi.tih.no Mon Feb 20 22:56:29 EST 1995
Article: 1477 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!rutgers!uwm.edu!math.ohio-state.edu!howland.reston.ans.net!pipex!sunic!trane.uninett.no!astfgl.edb.tih.no!pc013.kjemi.tih.no!bjorn
From: bjorn@kjemi.tih.no (Bjorn Ness)
Newsgroups: bionet.plants,bionet.general,bionet.mycology,bionet.agroforestry,sci.bio,sci.bio.tecnology
Subject: Mycorrhiza - production of plant growt/development hormones ???
Date: Sat, 18 Feb 1995 16:02:52
Organization: institutt for Kjemi
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Xref: bigblue.oit.unc.edu bionet.plants:5320 bionet.general:12588 bionet.mycology:1477 bionet.agroforestry:1205 sci.bio:21419

I searche for information about possible mycorrhizal production of plant 
growth hormones  (auxins, gibberrelin, cytokins).

I am well awere of the role mycorrhiza play in suporting the trees (/plants) 
with mineral components from the soil. But someone once wrote about plant 
hormone production as well.  Do anyone have som information on this ??


Please give me info bye E.mail: Bjorn@ik.hist.no

Thank You!!

Bjoern Arne Naess
Soer-Troendelag College 
dep. Chemistry, Chemical engineering/biotechnology 


From harris@bhc.com Mon Feb 20 22:56:30 EST 1995
Article: 1478 of bionet.mycology
Newsgroups: bionet.mycology
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From: harris@bhc.com (Bob Harris)
Subject: Re: Early Seattle mushrooms
Message-ID: <D43psJ.3K9@nbn.com>
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References: <3hi2d9$uj2@usenetw1.news.prodigy.com>  
 <3his5a$hrl@crl.crl.com>
X-Authenticated: harris on POP host miwok.nbn.com
Date: Thu, 16 Feb 1995 16:29:06 GMT

In article <3his5a$hrl@crl.crl.com>
kasplash@crl.com (Bob Galvan) writes:

> Here in Marin county, California Amanita pantherina has been common as
> cockroaches all January, and fading fast now as Amanita velosa pops up by
> every Coast Live Oak.  Lots of A. rubescens too.  Poor year for
> chanterelles here, though there have been good reports 50-100 miles south. 

Are you positive it is pantherina and not P. aspera which looks like it
and is more common here in Marin? Agree on Chants and boletes as well.

Bob Harris    harris@bhc.com
       
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       +++


From harris@bhc.com Mon Feb 20 22:56:32 EST 1995
Article: 1479 of bionet.mycology
Newsgroups: bionet.mycology
Path: bigblue.oit.unc.edu!concert!news-server.ncren.net!news.duke.edu!agate!overload.lbl.gov!emf.emf.net!hilbert.dnai.com!nbn!news
From: harris@bhc.com (Bob Harris)
Subject: Re: Growing mushrooms on Internet
Message-ID: <D43pu7.3M7@nbn.com>
X-Posted-From: InterNews 1.0.6@ncs-27.nbn.com
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References: <D3C95C.L2y@news.pfc.forestry.ca>  
 <3hjenk$6tu@newsbf02.news.aol.com>
X-Authenticated: harris on POP host miwok.nbn.com
Date: Thu, 16 Feb 1995 16:30:05 GMT

In article <3hjenk$6tu@newsbf02.news.aol.com>
wharrison@aol.com (WHARRISON) writes:

> You can also subscribe to the newsletter of the Colorado Mycological
> Society through the internet.  It's free, and chuck full of interesting
> articles, if I do say so myself.  I'm the editor.

Ok So how does one do that?

Bob Harris    harris@bhc.com
       
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From margolis@CMGM.STANFORD.EDU Mon Feb 20 22:56:33 EST 1995
Article: 1480 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!bloom-beacon.mit.edu!spool.mu.edu!agate!biosci!CMGM.STANFORD.EDU!margolis
From: margolis@CMGM.STANFORD.EDU (Peter Margolis)
Newsgroups: bionet.mycology
Subject: Mo bio question: Selection of bleo resistance
Date: 19 Feb 1995 13:12:10 -0800
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I'm hoping some mo bio type in this group can advise me on selection of
bleomycin resistance in Neurospora. Whenever I transform _N. crassa_ with
plasmids encoding bleomycin resistance (e.g. pAB520), I get heavy
background lawns, even though I include the drug in both top and bottom
agar, can confirm that the selection "works" (untransformed recipient is
indeed bleo sensitive), have checked the pH of the media, am following the
suggestions of the original paper on this selection. Anyone have experience
with this selection working? Thanks in advance. -Peter
P.S. Contrary to rumor, my brother did _not_ work on the set of The Highlander.



From coch@chuma.cas.usf.edu Mon Feb 20 22:56:34 EST 1995
Article: 1481 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!news-server.ncren.net!news.duke.edu!convex!darwin.sura.net!mother.usf.edu!chuma!coch
From: "Bruce Cochrane (BIO)" <coch@chuma.cas.usf.edu>
Newsgroups: bionet.mycology
Subject: Asst. Prof, Environmental Plant Biology
Date: Thu, 16 Feb 1995 16:31:18 -0500
Organization: University of South Florida
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                       Assistant Professor Position
                        Environmental Plant Biology
                        University of South Florida
                              Tampa, Florida

Department of Biology, University of South Florida.  9
month tenure-track position in Environmental Plant Biology
for Fall 1995.  Contingent upon funding.  Duties
include: teaching undergraduates in such areas as
ecology, evolution, physiology; supervising M.S. and
Ph.D students; graduate courses in specialty area.  We
seek a broadly-based person with interest in
terrestrial or wetlands ecosystem processes. 
Applicants with a Ph.D and training in ecology,
evolutionary biology, plant physiology, biochemistry,
or molecular biology are sought.  Needed by March 31,
1995: curriculum vitae, statement of teaching and
research interests, reprints, 3 letters of
recommendation.  Send to Search Committee, Department
of Biology, University of South Florida, 4202 E. Fowler
Avenue, Tampa, FL 33620-5150.  USF is an equal
opportunity/affirmative action/equal access
institution.  For disability accommodation, please
contact the Search Committee Chair five days in advance
(813-974-3250).

For Further Information, please contact Bruce J.
Cochrane, Ph. D. by email at coch@chuma.cas.usf.edu or
by phone at (813) 974-2087
	



From jmicales@facstaff.wisc.edu Mon Feb 20 22:56:35 EST 1995
Article: 1482 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!news-server.ncren.net!taco.cc.ncsu.edu!gatech!howland.reston.ans.net!news.sprintlink.net!uunet!in1.uu.net!caen!night.primate.wisc.edu!news.doit.wisc.edu!F180-080.net.wisc.edu!jmicales
From: jmicales@facstaff.wisc.edu (Jessie Micales)
Newsgroups: bionet.mycology
Subject: Re: Do I have cell wall fraction of yeast
Date: Thu, 16 Feb 1995 16:24:32
Organization: U.S. Forest Service
Lines: 31
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References: <cmody-1302951813280001@pc12.mid.ucalgary.ca>
NNTP-Posting-Host: f180-080.net.wisc.edu

>Can anybody tell me how to be resonably sure that I have separated the
>cell wall and cytosolic fractions of Cryptococcus neoformans. C.
>neoformans was grown in Sabs broth, killed with heat (autoclaved), washed
>and about 10^9 organisms placed in a glass bead mill [Bead Beater®]
>(volume of organisms 75 ml, beads 100 ml, buffer to fill chamber). Rotated
>one minute on, one minute off for 60 minutes on ice.  Disruption was
>complete by microscopic exam. A slow spin was done to remove debris.  The
>preparation was spum at 13,000 xg for 30 minutes, and the pellet and
>supernatant was collected. My reading would indicate that the pellet
>should contain the cell wall, the supernatant the cytosol.  How can I be
>more sure? Is there a simple assay that can be done to tell that my cell
>wall preparation has minimal contamination with cytosolic proteins and
>vice versa?

>Thanks
>Chris Mody
>cmody@acs.ucalgary.ca

You may want to redisperse and wash the pellet in buffer several times to 
remove any contaminating cytosolic material.  As for assays, good 
question!!!  There are lots of commercial protein assays available (check 
BioRad, for example), but I believe that cell walls can also contain some 
protein on their own (the concentration would probably be fairly low, 
however).  Many fungi also produce an extracellular mucilage (usually 
glucan) that can contain many extracellular proteins.  This glucan is 
actually an extension of the cell wall.  It can be removed from the cells by 
washing them in buffer before you break open the cells.  A little detergent 
in the buffer (such as Triton X-100) can also help remove the mucilage.

Good luck.  Please let me know if anyone e-mails you any better 
protocols!


From stamets1@aol.com Mon Feb 20 22:56:36 EST 1995
Article: 1483 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!howland.reston.ans.net!news.sprintlink.net!uunet!in1.uu.net!newstf01.news.aol.com!newsbf02.news.aol.com!not-for-mail
From: stamets1@aol.com (Stamets 1)
Newsgroups: bionet.mycology
Subject: Re: doing research on Ganoderma lucidium immunology paper
Date: 19 Feb 1995 20:13:32 -0500
Organization: America Online, Inc. (1-800-827-6364)
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Reply-To: stamets1@aol.com (Stamets 1)
NNTP-Posting-Host: newsbf02.mail.aol.com

RE: Ganoderma lucidum. 

At the last International Mycological Congress in August in Vancouver,
BC, a paper was presented by a group of Korean Researchers (physicians &
mycologists) who isolated a sub-fraction of a polysaccarhide of Ganoderma
lucidum, which, when exposed to HIV infected lymphocytes allowed for
prolongerd cell survival whereas those cells not exposed shortly died. 

This is particularly interesting to me because in dealing with NCI - the
positive results they have had are discarded as unapplicable due to the
size of the polysaccaharide molecules.  The prevailing belief is that
these compounds 
 can't be introduced into the cell without causing damage to
the cell wall membrane. The Korean work has isolated a  lower molecular
compound which obviously - in the case of lymphocytes - can be assimilated
and extends life of these immune cells. The testing procedure they used
was designed and approved by NCI, according to them. 

Their paper has not yet been published but will be summarized when the
proceedings are printed. 

Their path of isolation of these subfractions could easily be applied to
many
different Polypores.  I look forward to the results that these
subfractions and/or their combinations will have in  -in vivo-  studies in
the near future. The research in this subject is becoming much more
scientific credible as time goes on. 

Of course,  my company  has  lots of information in the forms of
books,etc.. 
and my lastest book Growing Gourmet & Medicinal Mushrooms is the first
book  (that I know of) to describe the cultivation of this species in
detail. 


paul stamets   mycomedia@aol.com


From bmtyler@UCDAVIS.EDU Mon Feb 20 22:56:37 EST 1995
Article: 1484 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!swrinde!howland.reston.ans.net!spool.mu.edu!olivea!biosci!UCDAVIS.EDU!bmtyler
From: bmtyler@UCDAVIS.EDU (Brett Tyler)
Newsgroups: bionet.mycology
Subject: Re: Mo bio question: Selection of bleo resistance
Date: 19 Feb 1995 14:28:49 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 33
Sender: daemon@net.bio.net
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Message-ID: <199502192229.OAA03917@chip.ucdavis.edu>
NNTP-Posting-Host: net.bio.net

>I'm hoping some mo bio type in this group can advise me on selection of
>bleomycin resistance in Neurospora. Whenever I transform _N. crassa_ with
>plasmids encoding bleomycin resistance (e.g. pAB520), I get heavy
>background lawns, even though I include the drug in both top and bottom
>agar, can confirm that the selection "works" (untransformed recipient is
>indeed bleo sensitive), have checked the pH of the media, am following the
>suggestions of the original paper on this selection. Anyone have experience
>with this selection working? Thanks in advance. -Peter
>P.S. Contrary to rumor, my brother did _not_ work on the set of The Highlander.

If you send me a detailed write up of your transformation and selection
procedure, I will have a look through it to see if there is anything I can
spot which may improve the selection.

In the meantime, you could simply try increasing the bleomycin
concentration and/or lowering the density of spheroplasts you are plating.
You can actually eliminate the bottom agar altogther if you want to save on
bleomycin.  Are you doing any outgrowth of the transformed sperplasts prior
to plating - this will make them much more resistant.





****************
Brett Tyler
Professor
Department of Plant Pathology
University of California, Davis
bmtyler@ucdavis.edu
****************




From lpoli@cello.gina.calstate.edu Mon Feb 20 22:56:39 EST 1995
Article: 1485 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!willis.cis.uab.edu!ddsw1!godot.cc.duq.edu!hudson.lm.com!news.pop.psu.edu!psuvax1!uwm.edu!spool.mu.edu!howland.reston.ans.net!usc!nic-nac.CSU.net!cello.gina.calstate.edu!swrl47.slip.csu.net!lpoli
From: Len A. Poli <lpoli@cello.gina.calstate.edu>
Newsgroups: bionet.mycology
Subject: Re: Boletus info requested
Date: 20 Feb 1995 16:55:10 GMT
Organization: NULL
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References: <3i65mg$paj@newsbf02.news.aol.com>
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Subject: Boletus info requested
From: Harlock999, harlock999@aol.com
Date: 18 Feb 1995 20:08:32 -0500
In article <3i65mg$paj@newsbf02.news.aol.com> Harlock999,
harlock999@aol.com writes:
>I am trying to find any information regarding Boletus edulis.  This
>includes possible innoculum sources, tips on culture, etc. etc. etc.
>
>Any information regarding this would be greatly appreciated.
>Harlock999@aol.com

You might try Mr. Paul Koski at Washington High School (Geary Blvd at 32
nd Ave.) in San Francisco CA, 94100. Paul is an officer in the San
Francisco Mycology Society and has participated in forays for Boletus.


From r3603207@CC.NTU.EDU.TW Mon Feb 20 22:56:40 EST 1995
Article: 1486 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!udel!news.sprintlink.net!howland.reston.ans.net!agate!biosci!CC.NTU.EDU.TW!r3603207
From: r3603207@CC.NTU.EDU.TW (r3603207)
Newsgroups: bionet.mycology
Subject: where is the good manual of fungi (molds)?
Date: 20 Feb 1995 06:09:51 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 2
Sender: daemon@net.bio.net
Distribution: world
Message-ID: <199502201411.WAA22196@ccsun10.cc.ntu.edu.tw>
NNTP-Posting-Host: net.bio.net

as title! Can anyone tell me where can I have a good exp handbook
of fungi or mold! Thanks!


From wharrison@aol.com Mon Feb 20 23:32:04 EST 1995
Article: 1487 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!howland.reston.ans.net!swiss.ans.net!newstf01.news.aol.com!newsbf02.news.aol.com!not-for-mail
From: wharrison@aol.com (WHARRISON)
Newsgroups: bionet.mycology
Subject: Re: Growing mushrooms on Internet
Date: 20 Feb 1995 09:48:46 -0500
Organization: America Online, Inc. (1-800-827-6364)
Lines: 19
Sender: root@newsbf02.news.aol.com
Message-ID: <3iaa4e$i9l@newsbf02.news.aol.com>
References: <D43pu7.3M7@nbn.com>
Reply-To: wharrison@aol.com (WHARRISON)
NNTP-Posting-Host: newsbf02.mail.aol.com

Bob,

Send email to sporescms@aol.com to subscribe.

I'd use my nifty Spores Afield signature but Otto Lang sent me the
following message complaing about it:

>Your .sig is getting a bit out of hand, Wayne, don't you think?
>We should all keep in mind by and for whom this newsgroup was
>originally intented and created. We amateurs are tolerated here
>and, I imagine, welcomed if we can keep our enthusiasm a bit more
>under control.

>Otto-- 
>Otto Lang, Mycological Society of Toronto <MST1@io.org> Fidonet
>1:250/228
>OR <ottolang@freenet.toronto.on.ca>

It's the first time the sig police have come after me....


From rwinder@PFC.Forestry.CA Tue Feb 21 13:37:17 EST 1995
Article: 1488 of bionet.mycology
Newsgroups: bionet.plants,bionet.general,bionet.mycology,bionet.agroforestry,sci.bio,sci.bio.tecnology
Path: bigblue.oit.unc.edu!concert!gatech!darwin.sura.net!spool.mu.edu!news.clark.edu!netnews.nwnet.net!news.pfc.forestry.ca!PFC.Forestry.CA!RWINDER
From: rwinder@PFC.Forestry.CA (Richard Winder)
Subject: Re: Mycorrhiza - production of plant growt/development hormones ???
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Reply-To: rwinder@PFC.Forestry.CA
Organization: Forestry Canada (Pacific Forestry Centre)
References: <bjorn.34.00100CBE@kjemi.tih.no>
Date: Mon, 20 Feb 1995 17:30:19 GMT
Lines: 22
Xref: bigblue.oit.unc.edu bionet.plants:5336 bionet.general:12604 bionet.mycology:1488 bionet.agroforestry:1207 sci.bio:21450

In article <bjorn.34.00100CBE@kjemi.tih.no>, bjorn@kjemi.tih.no (Bjorn Ness) writes:
>I searche for information about possible mycorrhizal production of plant 
>growth hormones  (auxins, gibberrelin, cytokins).
>
>I am well awere of the role mycorrhiza play in suporting the trees (/plants) 
>with mineral components from the soil. But someone once wrote about plant 
>hormone production as well.  Do anyone have som information on this ??
>
Most standard plant physiology texts will give you some basic information
on this- my dated copy of Salisbury & Ross, for example, explains that auxins,
as well as the cytokinins zeatin and zeatin riboside stimulate ectomycorrhizae
formation.  Plant hormone production by the fungal symbiont plays a role in
determining the degree of branching in a mycorrhizal root (increased branching
= more surface area for infection = greater transfer of nutrients, etc.)  If 
you need more info., there are many books on the general topic (and not a few
lurkers in this group that probably know a lot more about this).
-RSW


  RICHARD WINDER                    Title: Research Scientist
  Canadian Forest Service           Phone: (604) 363-0773
  Victoria, B.C.                    Internet: RWINDER@A1.PFC.Forestry.CA


From dgaunt@franklin.uhl.uiowa.edu Tue Feb 21 22:27:29 EST 1995
Article: 1489 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!news-server.ncren.net!news.duke.edu!godot.cc.duq.edu!newsfeed.pitt.edu!uunet!in1.uu.net!news.uiowa.edu!franklin.uhl.uiowa.edu!dgaunt
From: dgaunt@franklin.uhl.uiowa.edu (Dennis Gaunt)
Newsgroups: bionet.mycology
Subject: Re: My bro helped with Highlander series!
Date: 17 Feb 1995 20:01:38 GMT
Organization: University of Iowa, Iowa City, IA, USA
Lines: 20
Distribution: world
Message-ID: <3i2vb2$oml@nexus.uiowa.edu>
References: <D44IFG.Hx3@rco.qc.ca>
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X-Newsreader: TIN [version 1.2 PL2]

Jeff Haynes (koolaid@vircom.com) wrote:
: Hi!

: My brother was a Dolly Grip in the production of the HighLander series.
: Anyone that post to this newsgroup or read it work on it too?

: Get back to me via E-Mail please.

: THanks.

: Ryan

Boy, are you in the wrong place!
--
----------------------------------------------------------------------
| Dennis D. Gaunt                   | Internet: dgaunt@uhl.uiowa.edu | 
| The University of Iowa            |    Voice: (319) 335-4500       |
| Oakdale Research Campus           |      FAX: (319) 335-4555       |
| Iowa City, Iowa  52242            |                                |
----------------------------------------------------------------------


From mycochef@aol.com Tue Feb 21 22:27:30 EST 1995
Article: 1490 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!rutgers!gatech!udel!news.sprintlink.net!cs.utexas.edu!uunet!newstf01.news.aol.com!newsbf02.news.aol.com!not-for-mail
From: mycochef@aol.com (MYCOCHEF)
Newsgroups: bionet.mycology
Subject: Re: Growing mushrooms on Internet
Date: 20 Feb 1995 18:38:28 -0500
Organization: America Online, Inc. (1-800-827-6364)
Lines: 1
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NNTP-Posting-Host: newsbf02.mail.aol.com

just curious--are you bob harris of marin?


From mst1@io.org Tue Feb 21 22:27:31 EST 1995
Article: 1491 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!willis.cis.uab.edu!news.ecn.bgu.edu!psuvax1!uwm.edu!cs.utexas.edu!howland.reston.ans.net!pipex!bnr.co.uk!bmdhh222.bnr.ca!bcarh8ac.bnr.ca!bcarh189.bnr.ca!nott!torn!uunet.ca!uunet.ca!io.org!nobody
From: mst1@io.org (Otto Lang)
Newsgroups: bionet.mycology
Subject: Re: doing research on Ganoderma lucidium immunology paper
Date: 20 Feb 1995 08:46:07 -0500
Organization: Internex Online (io.org) Data: 416-363-4151  Voice: 416-363-8676
Lines: 29
Message-ID: <3ia6ev$fo4@ionews.io.org>
References: <intron-1802951527530001@tia1.eskimo.com>
NNTP-Posting-Host: grin.io.org

In article <intron-1802951527530001@tia1.eskimo.com>,
Dr. Karl Goldkamp <intron@eskimo.com> wrote:
>  Does any one have any information on Ganoderma alias Lingzhi, or
>Reishi.  I'm doning a Immunology paper  on it and have done medline
>search.  Im a student at a Naturopathic Medical school here in Seattle. 
>Though I know ganoderma is used for cancer in Japan I need to find studies
>that bring a western approach to it.  So far LZ-8 appears to be a isolated
>polysacchride of interest.  Any timps or leads or greatly appreciated.
>
>E-mail: intron@eskimo.com


Dr. Alice W. Chen, who describes herself as Ganoderma Mushroom Specialist,
gave a presentation to the Mycological Society of Toronto (MST) on Nov.21,
1994 on "Cultivation and Biomedical Importance of~Ganoderma lucidum~.

She is *not* connected ekectronically. Write her for information:

Alice W. Chen, Ph.D.
Specialty Mushroom Consultation
1730 Penfield Road
Penfield, N.Y. 14526, USA
Tel:(716) 381-0804

Hope this helps
Otto
-- 
Otto Lang, Mycological Society of Toronto <MST1@io.org> Fidonet 1:250/228
OR <ottolang@freenet.toronto.on.ca>


From biohelp Wed Feb 22 21:27:50 EST 1995
Article: 1493 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!rutgers!uwm.edu!cs.utexas.edu!howland.reston.ans.net!agate!biosci!internet!biosci!not-for-mail
From: biohelp (BIOSCI Administrator)
Newsgroups: bionet.mycology
Subject: UNSUBSCRIBING, BIOSCI ARCHIVES, ADDRESS DATABASE & BIOSCI FAQ
Date: 21 Feb 1995 02:00:27 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
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MAILING LIST NAME          USENET Newsgroup Name
-----------------          ---------------------
ACEDB-SOFT                 bionet.software.acedb
AGEING                     bionet.molbio.ageing
AGROFORESTRY               bionet.agroforestry
ARABIDOPSIS                bionet.genome.arabidopsis
ASCB                       bionet.prof-society.ascb
BIOCAN                     bionet.prof-society.cfbs
BIOFORUM                   bionet.general
BIO-INFORMATION-THEORY     bionet.info-theory
BIONAUTS                   bionet.users.addresses
BIONEWS                    bionet.announce
BIO-JOURNALS               bionet.journals.contents
BIO-MATRIX                 bionet.molbio.bio-matrix
BIOPHYSICAL-SOCIETY        bionet.prof-society.biophysics
BIOPHYSICS                 bionet.biophysics
BIO-SOFTWARE               bionet.software
BIOTHERMOKINETICS          bionet.metabolic-reg
BIO-WWW                    bionet.software.www
CARDIOVASCULAR-RESEARCH    bionet.biology.cardiovascular
CELEGANS                   bionet.celegans
CELL-BIOLOGY               bionet.cellbiol
CHLAMYDOMONAS              bionet.chlamydomonas
CHROMOSOMES                bionet.genome.chromosomes
COMPUTATIONAL-BIOLOGY      bionet.biology.computational
CSM                        bionet.prof-society.csm
CYTONET                    bionet.cellbiol.cytonet
DROSOPHILA                 bionet.drosophila
EMBL-DATABANK              bionet.molbio.embldatabank
EMF-BIO                    bionet.emf-bio
EMPLOYMENT                 bionet.jobs
EMPLOYMENT-WANTED          bionet.jobs.wanted
FASEB                      bionet.prof-society.faseb
GDB                        bionet.molbio.gdb
GENBANK-BB                 bionet.molbio.genbank
GENETIC-LINKAGE            bionet.molbio.gene-linkage
GRASSES-SCIENCE            bionet.biology.grasses
HIV-MOLECULAR-BIOLOGY      bionet.molbio.hiv
HUMAN-GENOME-PROGRAM       bionet.molbio.genome-program
IMMUNOLOGY                 bionet.immunology
INFO-GCG                   bionet.software.gcg
JOURNAL-NOTES              bionet.journals.note
METHODS-AND-REAGENTS       bionet.molbio.methds-reagnts
MICROBIOLOGY               bionet.microbiology
MOLECULAR-EVOLUTION        bionet.molbio.evolution
MOLECULAR-MODELLING        bionet.molec-model
MOLLUSC-MOLECULAR-NEWS     bionet.molbio.molluscs
MYCOLOGY                   bionet.mycology
NEUROSCIENCE               bionet.neuroscience
N2-FIXATION                bionet.biology.n2-fixation
PARASITOLOGY               bionet.parasitology
PHOTOSYNTHESIS             bionet.photosynthesis
PLANT-BIOLOGY              bionet.plants
POPULATION-BIOLOGY         bionet.population-bio
PROTEIN-ANALYSIS           bionet.molbio.proteins
PROTEIN-CRYSTALLOGRAPHY    bionet.xtallography
PROTISTA                   bionet.protista
RAPD                       bionet.molbio.rapd
SCIENCE-RESOURCES          bionet.sci-resources
STADEN                     bionet.software.staden
STRUCTURAL-NMR             bionet.structural-nmr
TROPICAL-BIOLOGY           bionet.biology.tropical
URODELES                   bionet.organisms.urodeles
VIROLOGY                   bionet.virology
WOMEN-IN-BIOLOGY           bionet.women-in-bio
YEAST                      bionet.molbio.yeast
ZBRAFISH                   bionet.organisms.zebrafish

Listing newsgroups on the comment: line is optional, of course.

Thanks again for your cooperation!



--------------- please cut here and return portion below ---------------

New information or Update to old record (enter N or U): 
date (DD-MM-YY): 
first name: 
middle initial: 
family name: 
job title: 
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FAX number: 
institution: 
address1: 
address2: 
address3: 
city: 
state/province: 
country: 
postal code: 
research interest: 
research interest: 
comment: 
comment: 
comment: 
comment: 
comment: 



From HGW1@psuvm.psu.edu Wed Feb 22 21:27:52 EST 1995
Article: 1494 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!rutgers!gatech!willis.cis.uab.edu!cs.utk.edu!stc06.CTD.ORNL.GOV!fnnews.fnal.gov!overload.lbl.gov!agate!library.ucla.edu!csulb.edu!nic-nac.CSU.net!charnel.ecst.csuchico.edu!olivea!spool.mu.edu!howland.reston.ans.net!news.cac.psu.edu!psuvm!hgw1
Organization: Penn State University
Date: Mon, 20 Feb 1995 13:46:08 EST
From: <HGW1@psuvm.psu.edu>
Message-ID: <95051.134608HGW1@psuvm.psu.edu>
Newsgroups: bionet.plants,bionet.mycology
Subject: Grad. Assistantships Available
Lines: 13
Xref: bigblue.oit.unc.edu bionet.plants:5355 bionet.mycology:1494

Graduate Assistantships.  The Penn State Plant Pathology Department has several
assistantships available for M.S. or Ph.D. students.  Faculty programs include
a diverse range of research in basic and applied plant pathology, including: fi
eld and greenhouse crop pathology, forest pathology, sustainable agriculture, m
olecular fungal taxonomy, mushroom culture and pathology, fungal genetics, epid
emiology, virology, plant responses to environmental stress, air pollution effe
cts to forest trees and ecosystems, root-specific metabolism, root-pathogen int
eractions, host-parasite interactions.  Excellent facilities available for fiel
d work and biochemical/molecular biology research.  Contact Dr. J.E. Ayers, Gra
duate Admissions Committee, Plant Pathology Department, 211 Buckhout Laboratory
, University Park, PA  16802  (Phone 814-865-7069, Fax 814-863-7217, E-mail htr
easte@psupen.psu.edu).  Affirmative Action/Equal Opportunity Employer; women an
d minorities encouraged to apply.


From GJBUNK@ccmail.monsanto.com Wed Feb 22 21:27:54 EST 1995
Article: 1495 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!rutgers!gatech!willis.cis.uab.edu!news.ecn.bgu.edu!psuvax1!gvls1!newsfeed.pitt.edu!godot.cc.duq.edu!news.duke.edu!agate!biosci!ccmail.monsanto.com!GJBUNK
From: GJBUNK@ccmail.monsanto.com
Newsgroups: bionet.mycology
Subject: Phytophthora meetings
Date: 20 Feb 1995 12:08:08 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 3
Sender: daemon@net.bio.net
Distribution: world
Message-ID: <9501207933.AA793317106@ccmail.monsanto.com>
NNTP-Posting-Host: net.bio.net

Recently I saw a message that referred to a Phytophthora meeting in Ireland. 
Can anyone supply me with more details on this meeting? Thanks for your help.



From prdhaas@HUGO.FSUFAY.EDU Wed Feb 22 21:27:56 EST 1995
Article: 1496 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!rutgers!gatech!willis.cis.uab.edu!nntp.msstate.edu!night.primate.wisc.edu!caen!uwm.edu!biosci!HUGO.FSUFAY.EDU!prdhaas
From: prdhaas@HUGO.FSUFAY.EDU
Newsgroups: bionet.mycology
Subject: motor oil decomposition
Date: 20 Feb 1995 15:22:52 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 4
Sender: daemon@net.bio.net
Distribution: world
Message-ID: <95022018114029@hugo.fsufay.edu>
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does anyone know if there are organisms in nature that break down motor oil
given enough time?  As you probably know many people dispose of oil in the
terrestrial environment. What eventualy  happens to this oil.  Will it 
end up in the water supply?


From SEELIGERC@AXE.HUMBOLDT.EDU Wed Feb 22 21:27:57 EST 1995
Article: 1497 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!howland.reston.ans.net!spool.mu.edu!olivea!biosci!AXE.HUMBOLDT.EDU!SEELIGERC
From: SEELIGERC@AXE.HUMBOLDT.EDU
Newsgroups: bionet.mycology
Subject: Re: where is the good manual of fungi (molds)?
Date: 21 Feb 1995 22:28:38 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 22
Sender: daemon@net.bio.net
Distribution: world
Message-ID: <Pine.3.89.9502212235.D539003436-0100000@AXE.HUMBOLDT.EDU>
References: <3ie429$2el@terrazzo.lm.com>
NNTP-Posting-Host: net.bio.net


> Anybody feel like putting a sharp illustrated key onto an
> interactive CD-RO

Yes, actually, I'd been fantasizing about that much of last semester, 
when I was taking a class in the microfungi.  It seems an anachronism to 
have to paw through a key when one is looking at an unfamiliar fungus 
with odd features.  If I'm looking at a fungus with phialophores which 
produce scolecospores, then I know that there can't be more than a very 
few genera that do this.  It would be a great improvement to simply click 
on 'type of conidiogenous cell' and 'spore/conidia type', and let the 
computer present me with a list of matches.  This list could then be 
searched manually (if short enough), or pared down by specifying more 
traits, or perhaps a key could be generated for the remaining taxa.

As a former (reformed?) software engineer, I think this would not be 
difficult (well, the key generation might be).  Is there a good source of 
digitized microphotographs around??

Curt
SeeligerC@Axe.Humboldt.Edu



From burns@sasa.gov.uk Wed Feb 22 21:27:59 EST 1995
Article: 1498 of bionet.mycology
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From: burns@sasa.gov.uk (Robert Burns)
Newsgroups: bionet.mycology
Subject: Phytophthora diagnostics
Date: 21 Feb 1995 09:49:21 -0800
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Hi,
   we are about to start some serological (monoclonal) work on the
detection of Phytophthora species causing soft fruit diseases. We will
be investigating a number of immunisation systems and also several
antigens (processed and native). Anybody working along similar 
lines who would like to correspond?


Robert


Robert Burns

Monoclonal Antibody Unit
Scottish Agricultural Science Agency
East Craigs
Edinburgh
Scotland

burns@sasa.gov.uk




From marcus@radair.win-uk.net Wed Feb 22 21:28:01 EST 1995
Article: 1499 of bionet.mycology
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Newsgroups: bionet.mycology
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Reply-To: marcus@radair.win-uk.net (Marcus King)
From: marcus@radair.win-uk.net (Marcus King)
Date: Mon, 20 Feb 1995 22:27:52 GMT
Subject: information on Psilocybe cult in Mexico
Lines: 8

I'm an undergraduate studying mycology, and am interested in
finding out more along the lines of ethnomycology. I would be
grateful if anyone knows anything about the Mexican mushroom
cults, or can point me in the direction of useful references. 

        Please send any info to bs1cah@bath.ac.uk

Charlotte Hedges

Marcus King                           _______        _     
Bath, Avon, England.                 /       \     o/ \\  
Email marcus@radair.win-uk.net        \  o  /      <\__,\\ 
Phone 01225 428356                       |          ">    |
                                         ^            `          
                                      Paraglide  &  Climb


From mspear@telerama.lm.com Wed Feb 22 21:28:03 EST 1995
Article: 1500 of bionet.mycology
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From: mspear@telerama.lm.com (mspear)
Newsgroups: bionet.mycology
Subject: Re: where is the good manual of fungi (molds)?
Date: 21 Feb 1995 20:29:45 -0500
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r3603207 (r3603207@CC.NTU.EDU.TW) wrote:
> as title! Can anyone tell me where can I have a good exp handbook
> of fungi or mold! Thanks!

I've been looking myself.  I assume you mean a taxonomical key to the
hyphomycetes.  I wish I had a great one to reccomend but I can point
only to a few that are pretty good.

The classic is Burnette, a blue spiral bound book found in a lot of
labs but no longer in print in think.  

I've got "General of the Hyphomycetes" by Carmichael et al.  Nice
drawings but no real key.

Von Arx is ok but has a very difficult key.

We just got a new one with a title something like, "A Pictorial Atlas
of Soil Fungi"  really great microphotography  (mycophotography?)  but
also a very difficult key.

I don't know your technical level but there is a very basic listing of
 the most common contaminats in recreational mushroom growing in
Stamete's first book.  

Hope this helps.  I hope someone posts something else that's really
great.  Anybody feel like putting a sharp illustrated key onto an
interactive CD-RO

    ---- Mark


From VPGH51A@prodigy.com Wed Feb 22 21:28:05 EST 1995
Article: 1501 of bionet.mycology
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From: VPGH51A@prodigy.com (Jim Berlstein)
Newsgroups: bionet.mycology
Subject: Re: Help!
Date: 21 Feb 1995 16:43:35 GMT
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Dustin,
Psilocybe cubensis SPORES are probably legal, as they contain no 
psilocybin.  If you do grow the mushrooms, however, they contain 
psilocybin, a reasonably effective hallucinogen similar to LSD.  Then you 
will be guilty of not only posession of a controled substance, but the 
manufacture of a controled substance.  Under today's draconian and 
misguided drug laws, this could subject you to jail time and the possible 
confiscation of your home, just as if you were operating a large 
methamphetamine factory.  In practice, I do not think the laws against 
possesion of psilocybin are enforced to this absurd level, yet I believe 
it is possible in theory. If all you want out of a mushroom is a good 
meal, I would reccomend some other species, preferbly one not in the 
psilocybe genus.  Best of luck.


-
  JIM BERLSTEIN  VPGH51A@prodigy.com





From SEELIGERC@AXE.HUMBOLDT.EDU Wed Feb 22 21:28:07 EST 1995
Article: 1502 of bionet.mycology
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From: SEELIGERC@AXE.HUMBOLDT.EDU
Newsgroups: bionet.mycology
Subject: Re: motor oil decomposition
Date: 21 Feb 1995 22:11:58 -0800
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On Mon, 20 Feb 1995 prdhaas@HUGO.FSUFAY.EDU wrote:

> does anyone know if there are organisms in nature that break down motor oil
> given enough time?  As you probably know many people dispose of oil in the
> terrestrial environment. What eventualy  happens to this oil.  Will it 
> end up in the water supply?

A grad student here recently worked on bioremediation of diesel spills.  
In greenhouse trials comparing unfertilized soil, fertilized soil, a 
specially engineered bacterium, the special bacteria with fertilizer, and 
a control, she found no significant difference between the non-control 
treatments, and a significant increase in rate of diesel breakdown over a 
three month period.  After three months, the soil that had been 
fertilized had gone from 5000 ppm diesel to under 100 (possibly under 10, 
I forget...)

Hope this helps,
	cur
	seeligerc@axe.humboldt.edu



From pb019@CSC.ALBANY.EDU Wed Feb 22 21:28:09 EST 1995
Article: 1503 of bionet.mycology
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From: pb019@CSC.ALBANY.EDU (Partha Banerjee)
Newsgroups: bionet.mycology
Subject: Possible job workshop at MSA meetings in San Diego
Date: 21 Feb 1995 11:47:21 -0800
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Re:  Comments from mspear@telerama.lm.com (mspear)

I thank you for your comment.  I am trying to organize a workshop on job
matters at the August MSA meetings in San Diego.  Would you be interested
to participate in the workshop as a speaker if I can finally put it
together (at this point, it's very tentative)?  Otherwise, any further
advice on this subject would be highly appreciated.

>As an employer I agree.  We've had trouble recruiting people,
>especially at the Bachelor's and Master's level.  Although we are not
>currently seeking new co-workers it would be nice to have a resource
>like this.

>    ---- Mark




From chuy@westminster.ac.uk Thu Feb 23 11:40:34 EST 1995
Article: 1504 of bionet.mycology
Newsgroups: bionet.mycology
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From: chuy@westminster.ac.uk (Yiuwai Chu)
Subject: Fungal Genetics - Asilomar
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Hi all,

I am going to the Fungal Genetics Meeting on 21st - 26th of March at
Asilomar. I have never been to the States before and would like to visit 
some molecular biology laboratory in the San Fransico area afterwards.
It doesn't have to be fungal. I just want to see how things are run 
and perhaps exchange some ideas. 

I would be very grateful if anyone out there could help!

Please email me directly.

Many thanks in advance.

--
Yiu-Wai CHU (friends call me Alf)
School of Biological and Health Sciences
University of Westminster
115 New Cavendish Street, London W1M 8JS
United Kingdom	tel. 0171-911 500 x3718 fax. 0171-911 5087







From chuy@westminster.ac.uk Thu Feb 23 11:40:37 EST 1995
Article: 1505 of bionet.mycology
Newsgroups: bionet.mycology
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From: chuy@westminster.ac.uk (Yiuwai Chu)
Subject: Looking for Dr Matt Tobin
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Hi all,

Does anyone know the whereabout of Dr Matthew Tobin who used to work
in the Dyson Perrin Laboratories at Oxford?

Please pass this message to him or email me direct.

Many thanks.

--
Yiu-Wai CHU (friends call me Alf)
School of Biological and Health Sciences
University of Westminster
115 New Cavendish Street, London W1M 8JS
United Kingdom	tel. 0171-911 500 x3718 fax. 0171-911 5087







From SEIFERTK@NCCCOT.AGR.CA Thu Feb 23 11:40:39 EST 1995
Article: 1506 of bionet.mycology
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From: SEIFERTK@NCCCOT.AGR.CA
Newsgroups: bionet.mycology
Subject: Computerized mould identification
Date: 22 Feb 1995 07:08:59 -0800
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Recent posts to this newsgroup about problems identifying moulds, and
the need for a good mould identification manual lead me to suggest
several possibilities.

1.  Bryce Kendrick is selling a computerized synoptic key to all known
hyphomycete genera for about $80.  It is based on a database he created
using the free form database program AskSam.  It is not
point and click and the user is expected to memorize approximately ten
descriptor abbreviations and use these in combination with key words.
The thing is that this key is fast, it works, and it is up to date.
You can contact Bryce at us707@freenet.victoria.bc.ca

2.  There are several excellent books available that combine
top quality illustrations and good keys but are not 'monographs' of all
hyphomycete genera.  These include:

Samson, R. A. and E. S. Hoekstra. 1994.  Introduction to Food Borne Fungi.
published by Centraalbureau voor Schimmelcultures, P. O. Box 273, 3740
AG Baarn, the Netherlands.  This book, now in its fourth edition, includes
about 100 species.  The trick is that these are the most common 100 moulds
in most biodeterioration situations.

Wang, C. J. K. and R. A. Zabel. 1990.  Identificaiton manual for Fungi
from Utility Poles in the eastern United States.  published by the
American Type Culture Collection, 12301 Parklawn Drive, Rockville, MD
20852.  More useful than the title might imply.  Includes descriptions
and illustrations of common microfungi (basidiomycetes, ascomycetes and
anamorphic fungi) on wood.

Domsch, K. H., W. Gams and T.-H. Anderson. 1993 (reprint).  Compendium
of soil fungi.  reprinted by IHW-Verlag, Bert-Brecht-Str. 18, D-85386
Eching, Germany.  This classic book (originally published in 1980) 
includes more than 200 species, with brief literature reviews on the
biological and biochemical properties of each species.  Worth the price
for *any* lab working with microfungi.

And for those who must deal with Penicillium and Aspergillus but don't
want to wade through the monographs...

Pitt, J. I. 1988.  A laboratory guide to common Penicillium species.

			and

Klich, M. A. and J. I. Pitt. 1988.  A laboratory guide to common
Aspergillus species and their teleomorphs.

Both available from J. I. Pitt, CSIRO, Division of Food Processing,
P. O. Box 52, North Ryde, NSW 2113, Australia.

3.  If you are serious about contributing to our knowledge of anamorphic
fungi, or to helping develop information resources that can be used by
people with an interest in these fungi, join AnaNet.  This is an 
organization (rather loose...) of about 150 individuals world wide
who share this common interest.  Contact me at seifertk@ncccot.agr.ca
for more information.

Keith Seifert


From stamets1@aol.com Thu Feb 23 11:40:41 EST 1995
Article: 1507 of bionet.mycology
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From: stamets1@aol.com (Stamets 1)
Newsgroups: bionet.mycology
Subject: Re: Help!
Date: 22 Feb 1995 10:07:49 -0500
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I believe viable spores of active Psilocybes
 are clearly ILLEGAL. If the spores can be germinated, 
they will generate psilocybin and/or psilocin, two
indoles specifically restricted by law. (Schedule 1; Title 21).

 


From daka@aloha.com Thu Feb 23 11:40:42 EST 1995
Article: 1508 of bionet.mycology
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From: daka@aloha.com (_doc_)
Newsgroups: bionet.mycology
Subject: FUNGUS Digest
Date: 21 Feb 1995 14:09:32 -1000
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Does anyone know how to unsubscribe from the FUNGUS Digest Mailing List?
If so please mail me or post.

____________________________________________________________________________
| 'And God said, Behold,  | "I don't practice what I preach, because I'm   |
|  I have given you every |  not the kind of person whom I'm preachin'     |
|  herb bearing seed,     |  to!"   --Church of the SubGenius <Praise Bob!>|
|  which is upon the face |                                                |
|  of the earth.'         |      Finger me for my PGP Public Key           |
|          --Genesis 1:29 | Aloha from Hawaii! _doc_ <daka@mango.aloha.com>|
----------------------------------------------------------------------------



From pokerholm@aol.com Thu Feb 23 11:40:44 EST 1995
Article: 1509 of bionet.mycology
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From: pokerholm@aol.com (P Okerholm)
Newsgroups: bionet.mycology
Subject: Re: Cooking Mushrooms?
Date: 21 Feb 1995 19:10:11 -0500
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Steve,
    I have two sources for some of the books mentioned  (and one that
hasn't been)
.
         Fingi Perfecti 
         P.O. Box 7634 
        Olympia, WA 98507
        (206) 426-9292

        Field & Forest Products
        N3296 Kozuzek Road
        Peshtigo, WI 54157
        (715) 582-4997

    FP carries Joes Book of Mushroom Cookery @ $14.95 and Hope's Mushroom
Cookbook @ $19.95.

    F&FP carries Joes...@ $17.50 and "The Shiitake Way" by Snyder @ $8.00.


    "Joes..." and "Hopes..." have already been mentioned. The Shiitake Way
is a health food/vegetarian book and contains some rather interesting
gourmet dishes. My son and I tried one of these recipes; by the time we
got through buying all the ingredients we could have gone out to dinner
for less cash but it was very good. It was a Tofu Stroganoff with a cashew
based paste. I recommend it.  


From mspear@telerama.lm.com Thu Feb 23 11:40:46 EST 1995
Article: 1510 of bionet.mycology
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From: mspear@telerama.lm.com (mspear)
Newsgroups: bionet.mycology
Subject: Re: Boletus info requested
Date: 21 Feb 1995 20:18:13 -0500
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Harlock999 (harlock999@aol.com) wrote:
> I am trying to find any information regarding Boletus edulis.  This
> includes possible innoculum sources, tips on culture, etc. etc. etc.

> Any information regarding this would be greatly appreciated.
> Harlock999@aol.com

B. edulus is an ectomycorrhizal fungus.  To grow it you first need to
prepare a rather exotic medium then inoculate tree seedlings.  Next
you wait 3-6 years while your trees grow and if you are lucky and
skillful you can get mushrooms.

The only successful cultivated bolete patch I've ever seen is at INRA,
in Bordeaux, France.  Check the literature.

To my knowledge nobody has ever gotten commercial success with this
but there is always a first person.  Like as not the person that does
it will be someone who does not listen to the skeptics.

Good luck, let us know if it works.

     ---- Mark


From slmanley@csulb.edu Thu Feb 23 21:15:29 EST 1995
Article: 1511 of bionet.mycology
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From: slmanley@csulb.edu (SLM)
Newsgroups: bionet.mycology
Subject: marine mycology references
Date: 22 Feb 1995 19:47:54 GMT
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would you please relay to me a few recent reviews or papers on marine fungi?

thanks in advance,

---------------------------------------
Steven L. Manley, slmanley@csulb.edu 
Dept. of Biol. Sci., Calif. St. Univ.
Long Beach, Ca USA 
90840-4806 310-985-1568; FAX 310-985-2315



From VPGH51A@prodigy.com Thu Feb 23 21:15:31 EST 1995
Article: 1512 of bionet.mycology
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From: VPGH51A@prodigy.com (Jim Berlstein)
Newsgroups: bionet.mycology
Subject: Re: Help!
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LegaL status of psilocybe spores-

     I talked to my friendly defense lawyer friend last night.  This is 
what I learned:

      Spores, if they contain no psilocybin/psilocin AND they contain no 
restricted chemical precursers to these compounds, then they are not 
illegal, but.......
purchasing them, or having them can probably be used to show an intent to 
manufacture psilocybin; and an overzealous police force, with visions of 
confiscated property and big police parties, can charge you with 
Conspiricy to manufacture a controled substance.  If  you happen to order 
spores and a growing kit from a false company that is really a sting 
operation, then, assuming you used your home phone, your home is a 
location that has facilitated this conspiracy, and is subject to 
confiscation.  Hard to believe but true. So, while the legal status of 
spores is still debatable as far as I know, they can clearly get you into 
trouble either way. I apologize for any inaccurate information I may have 
conveyed.


-
  JIM BERLSTEIN  VPGH51A@prodigy.com





From jolivas@CYBERGATE.COM Thu Feb 23 21:15:34 EST 1995
Article: 1513 of bionet.mycology
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From: jolivas@CYBERGATE.COM (John L. Olivas)
Newsgroups: bionet.mycology
Subject: Re: where is the good manual of fungi (molds)?
Date: 22 Feb 1995 18:21:51 -0800
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>as title! Can anyone tell me where can I have a good exp handbook
>of fungi or mold! Thanks!
>

I've found a good one for mushrooms, cheap, compact. Excelent color plate 
guide, and anytomical key. Also includes info on habitat, season, range, 
wheather edible and look alikes.....

        The Audubon Society Field Guide To North American Mushrooms
        AU: Gary H. Lincoff (Pres. North American Mycological Assn)
        SO: Random House
        Cost: $18.00

I found mine at CostCo Wholesale for $9.99
* John L. Olivas                             -     Phone 209-269-1152        
         *
* Technical Representative         -     Office 800-USA-BIO1               *
* BioScientific, Inc.                        -    Fax 209-439-8639           
            *
* 3574 W. Escalon Avenue          -    Email jolivas@cybergate.com *
* Fresno, California 93711            -                                      
                    *



From 74557.3134@CompuServe.COM Thu Feb 23 21:15:35 EST 1995
Article: 1514 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!rutgers!uwm.edu!vixen.cso.uiuc.edu!howland.reston.ans.net!news.sprintlink.net!uunet!news.inhouse.compuserve.com!news.production.compuserve.com!news
From: Tim Johnson <74557.3134@CompuServe.COM>
Newsgroups: bionet.biology.tropical,bionet.molbio.bio-matrix,bionet.mycology,bionet.plants,misc.health.alternative
Subject: Database of Plants & Their Uses
Date: 22 Feb 1995 22:01:53 GMT
Organization: Overmind
Lines: 24
Message-ID: <3igc8h$7fa$1@mhade.production.compuserve.com>
Xref: bigblue.oit.unc.edu bionet.biology.tropical:499 bionet.molbio.bio-matrix:500 bionet.mycology:1514 bionet.plants:5404 misc.health.alternative:23512

I just finished the first version of Herbage, a shareware program 
for DOS. This program allows searches of a very large database of 
plants from all around the world. You can search in 10 
categories, including alkaloid and nutrition content, native 
range and habitat, traditional uses for specific illnesses, and 
U.S. mail-order sources. This program is the result of two years 
of research using object-oriented and relational data models. As 
far I as know, this is the first program to make use of this 
technology for the collection of plant information.

It is available: 

1. Compuserve \ New Age Forum \ IBM PC Files
2. Compuserve \ Gardening \ New Uploads
3. Compuserve \ Holistic Health \ Herbs & Plants
4. America Online \ Learning & Reference 
     \ Educational Software \ PC/MS DOS Educational Software 
     \ New Education Files

Within a week or so, it will be available on the FTP site:

oak.oakland.edu \ simtel \ msdos \ food

- Tim Johnson


From mflorian@sun10.inf.unitn.it Fri Feb 24 21:56:10 EST 1995
Article: 1515 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!howland.reston.ans.net!pipex!oleane!jussieu.fr!univ-lyon1.fr!serra.unipi.it!sirio.cineca.it!gopher
From: Marco Floriani <mflorian@sun10.inf.unitn.it>
Newsgroups: bionet.mycology
Subject: Fries's S.M. reprint
Date: 23 Feb 1995 12:11:47 GMT
Organization: Cineca
Lines: 16
Message-ID: <3ihu23$mv0@sirio.cineca.it>
NNTP-Posting-Host: sunc7.inf.unitn.it


   The Mediterranean Mycology European Confederation (C.E.M.M.) has
reprinted some of the first books written by E.M. Fries, specifically
"Observationes Mycologicae", "Systema mycologicum" and "Elenchus fun-
gorum". This reprint consists of three hard-bound volumes, each con-
sisting of 1000 pages about. They can be bought at an extremely nice
price until March 1995 (about 60$!).

   Note that these books are essential for all the mycologists and
that until today they where very difficult to find.

   If you want to know some more, please E-mail me.

Marco Floriani
mflorian@sun10.inf.unitn.it



From lmelvill@uoguelph.ca Fri Feb 24 21:56:11 EST 1995
Article: 1516 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!bloom-beacon.mit.edu!usc!nic-nac.CSU.net!newshub.sdsu.edu!ucsnews!sol.ctr.columbia.edu!proto.ida.org!bah.com!uunet!in1.uu.net!news.sprintlink.net!howland.reston.ans.net!torn!ccshst05.cs.uoguelph.ca!ccshst01.cs.uoguelph.ca!lmelvill
From: lmelvill@uoguelph.ca (Lewis Melville)
Newsgroups: bionet.plants,bionet.general,bionet.mycology,bionet.agroforestry,sci.bio,sci.bio.tecnology
Subject: Re: Mycorrhiza - production of plant growt/development hormones ???
Followup-To: bionet.plants,bionet.general,bionet.mycology,bionet.agroforestry,sci.bio,sci.bio.tecnology
Date: 23 Feb 1995 19:17:10 GMT
Organization: University of Guelph
Lines: 11
Message-ID: <3iimvm$lg0@ccshst05.cs.uoguelph.ca>
References: <bjorn.34.00100CBE@kjemi.tih.no> <D4B7AK.H4s@news.pfc.forestry.ca>
NNTP-Posting-Host: ccshst01.cs.uoguelph.ca
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Xref: bigblue.oit.unc.edu bionet.plants:5412 bionet.general:12670 bionet.mycology:1516 bionet.agroforestry:1221 sci.bio:21523

Richard Winder (rwinder@PFC.Forestry.CA) wrote:
: In article <bjorn.34.00100CBE@kjemi.tih.no>, bjorn@kjemi.tih.no (Bjorn Ness) writes:
: >I searche for information about possible mycorrhizal production of plant 
: >growth hormones  (auxins, gibberrelin, cytokins).

	Try the mycology newsgroup. Also, if you mail a subscription 
request to listserv@uoguelph.ca with the message SUBSCRIBE MICRONET you 
will be added to the micronet mailing list for mycorrhiza researchers 
around the world. You could then ask everyone on the list (150 
researchers). 
good luck . lewis melville.


From mbleeker@euronet.nl Fri Feb 24 21:56:13 EST 1995
Article: 1517 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!bloom-beacon.mit.edu!hookup!news.moneng.mei.com!howland.reston.ans.net!EU.net!sun4nl!news.euro.net!p88.euronet.nl!mbleeker
From: mbleeker@euronet.nl (Marco_Bleeker)
Newsgroups: bionet.biology.tropical,bionet.molbio.bio-matrix,bionet.mycology,bionet.plants,misc.health.alternative
Subject: Re: Database of Plants & Their Uses
Date: Fri, 24 Feb 1995 02:33:10
Organization: Euronet Internet
Lines: 10
Message-ID: <mbleeker.109.00028D9D@euronet.nl>
References: <3igc8h$7fa$1@mhade.production.compuserve.com>
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Xref: bigblue.oit.unc.edu bionet.biology.tropical:503 bionet.molbio.bio-matrix:501 bionet.mycology:1517 bionet.plants:5419 misc.health.alternative:23592

>I just finished the first version of Herbage, a shareware program 
>for DOS. This program allows searches of a very large database of 
>plants from all around the world.

  Hi Tim
  Which very large databases - how do you connect up ?  Is Herbage an 
empty RDBMS, or are there data in it already ?
  Marco




From msachs@ADMIN.OGI.EDU Sun Feb 26 21:39:35 EST 1995
Article: 1518 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!hearst.acc.Virginia.EDU!portal.gmu.edu!europa.eng.gtefsd.com!ra.nrl.navy.mil!news.pop.psu.edu!hudson.lm.com!newsfeed.pitt.edu!gatech!howland.reston.ans.net!spool.mu.edu!olivea!biosci!ADMIN.OGI.EDU!msachs
From: msachs@ADMIN.OGI.EDU (Matthew Sachs)
Newsgroups: bionet.mycology
Subject: Re: Insertional/Tn mutagenesis in Neurospora?
Date: 24 Feb 1995 11:39:41 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
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NNTP-Posting-Host: net.bio.net

>Greetings. In random discussions, several of us Neurosporologists have
>remarked on the challenge presented by cloning genes in this filamentous
>fungus. This stumbling block would be dramatically reduced by insertional
>mutagenesis, but this aspect of the system remains undeveloped. Attempts in
>this lab to apply REMI in Neurospora have been unfruitful, though rumor has
>it that this approach works in "every other" fungus. Has anyone had recent
>luck or insights into using any sort of insertional or (especially)
>transposon mutagenesis in Neurospora or parallel filamentous fungi?
>Hopefully we can come up with some likely approaches and/or confer on this
>topic here and/or at the Fungal Genetics Conference next month. Any
>thoughts...?
>-Peter Margolis

Re:  Insertional mutagenesis in Neurospora:

S. Kang and R. L. Metzenberg (1993), Genetics 133(2): 193-202.
Insertional mutagenesis in Neurospora crassa: cloning and molecular
analysis of the preg+ gene controlling the activity of the transcriptional
activator NUC-1.

Abstract:
The transcriptional activator NUC-1 controls the transcription of the genes
for phosphorus acquisition enzymes, and its activity is regulated by the
negative regulatory factors, PREG and PGOV In this report, we describe the
cloning and molecular analysis of the preg+ gene. In Neurospora crassa, as
in higher eukaryotes, transformation frequently results in nonhomologous
integration of transforming DNA. Insertion of transforming DNA into host
genes mutates the gene and provides a molecular tag for cloning it. We
obtained two mutants that have an insertion in the preg+ and pgov+ genes,
respectively, among 2 x 10(5) transformants. The preg+ gene was cloned by
screening a Neurospora genomic DNA library with DNA sequences flanking the
transforming DNA of the rescued plasmid. Northern analysis showed that the
transcription of the preg+ gene is not regulated by phosphate. The
carboxy-terminal half of PREG shows strong homology with Saccharomyces
cerevisiae PHO80 whose function is analogous to that of PREG. The pregc
mutations are located in the well conserved residues which may directly
interact with the residues in the regulatory domain of NUC-1.




-----------------------------------------------------------
Matthew Sachs
Department of Chemistry, Biochemistry and Molecular Biology
Oregon Graduate Institute of Science and Technology
20000 NW Walker Road
P.O. Box 91000
Portland, OR  97291-1000
503 690-1487 Phone
503 690-1464 Fax
msachs@admin.ogi.edu




From jirelan@darkwing.uoregon.edu Sun Feb 26 21:39:37 EST 1995
Article: 1519 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!howland.reston.ans.net!vixen.cso.uiuc.edu!news.uoregon.edu!darkwing!jirelan
From: jirelan@darkwing.uoregon.edu (Jeffrey T Irelan)
Newsgroups: bionet.mycology
Subject: Re: Insertional/Tn mutagenesis in Neurospora?
Date: 25 Feb 1995 02:43:30 GMT
Organization: University of Oregon
Lines: 11
Distribution: world
Message-ID: <3im5gi$e5h@pith.uoregon.edu>
References: <199502241855.KAA18832@cmgm.Stanford.EDU>
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	In addition to Kang & Metzenberg, see Arganoza et al. Genetics 
137(3):731-742 (July 1994).


*-----------------------------*
Jeff Irelan
Institute of Molecular Biology
University of Oregon
Eugene, OR 97403-1229
jirelan@darkwing.uoregon.edu


From DEYOUNGR@ONRSVI.AGR.CA Mon Feb 27 21:51:48 EST 1995
Article: 1520 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!rutgers!uwm.edu!spool.mu.edu!olivea!biosci!ONRSVI.AGR.CA!DEYOUNGR
From: DEYOUNGR@ONRSVI.AGR.CA
Newsgroups: bionet.mycology
Subject: Re: viral extraction from fungi
Date: 24 Feb 1995 12:11:56 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 8
Sender: daemon@net.bio.net
Distribution: world
Message-ID: <01HNFK91KQCI004092@GW.AGR.CA>
NNTP-Posting-Host: net.bio.net


Hello, we are looking for a 'tried and true' method for purifying viral 
particles from filamentous fungi.  Any suggestions?

	Thank you in advance.

deyoungr@onrsvi.agr.ca



From rwinder@PFC.Forestry.CA Tue Feb 28 11:37:44 EST 1995
Article: 1521 of bionet.mycology
Newsgroups: bionet.mycology
Path: bigblue.oit.unc.edu!concert!news-feed-1.peachnet.edu!news.duke.edu!convex!cs.utexas.edu!howland.reston.ans.net!news.sprintlink.net!alfa02.medio.net!netnews.nwnet.net!news.pfc.forestry.ca!PFC.Forestry.CA!RWINDER
From: rwinder@PFC.Forestry.CA (Richard Winder)
Subject: Re: Boletus info requested
X-Nntp-Posting-Host: pfc.pfc.forestry.ca
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Reply-To: rwinder@PFC.Forestry.CA
Organization: Forestry Canada (Pacific Forestry Centre)
References: <3i65mg$paj@newsbf02.news.aol.com>,<3ie3cl$291@terrazzo.lm.com>
Date: Wed, 22 Feb 1995 19:08:54 GMT
Lines: 42

In article <3ie3cl$291@terrazzo.lm.com>, mspear@telerama.lm.com (mspear) 
writes:
>Harlock999 (harlock999@aol.com) wrote:
>> I am trying to find any information regarding Boletus edulis.  This
>> includes possible innoculum sources, tips on culture, etc. etc. etc.
>
>> Any information regarding this would be greatly appreciated.
>> Harlock999@aol.com
>
>B. edulus is an ectomycorrhizal fungus.  To grow it you first need to
>prepare a rather exotic medium then inoculate tree seedlings.  Next
>you wait 3-6 years while your trees grow and if you are lucky and
>skillful you can get mushrooms.
>
>The only successful cultivated bolete patch I've ever seen is at INRA,
>in Bordeaux, France.  Check the literature.

It is possible to produce basidiocarps of B. edulis in axenic culture.
Use a deep dish or big jar or flask with modified Hagem/Modess medium:

5 g    Glucose
5 g    Malt extract (Difco)
0.5 g  KH2PO4
0.5 g  MgSO4.7H2O
0.5 g  NH4Cl
0.5 ml Ferric citrate (1%)
15 g   Bacto-agar (Difco)
1 l    H2O, distilled

Q.v.:

McLaughlin, David J. 1970. Environmental control of frutibody development in
Boletus rubinellus in axenic culture. Mycologia 62:307-331.

You can also find some related citations in the latest MycoInfo.  -RSW


Good luck

  RICHARD WINDER                    Title: Research Scientist
  Canadian Forest Service           Phone: (604) 363-0773
  Victoria, B.C.                    Internet: RWINDER@A1.PFC.Forestry.CA


From grunwald@netcom.com Tue Feb 28 11:37:48 EST 1995
Article: 1522 of bionet.mycology
Newsgroups: bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!howland.reston.ans.net!ix.netcom.com!netcom.com!grunwald
From: grunwald@netcom.com (Stefan Gruenwald)
Subject: New Biotechnology Discussion Group
Message-ID: <grunwaldD4L6HL.Hn6@netcom.com>
Keywords: Biotechnology, Research, Industry, Academia, Ideas, Jobs
Organization: NETCOM On-line Communication Services (408 261-4700 guest)
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Date: Sun, 26 Feb 1995 02:48:57 GMT
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Sender: grunwald@netcom.netcom.com

Dear colleagues:

A novel international Biotechnology Discussion List (BIZ-BIOTECH) has 
been created. 

Subscription address:        listserv@netcom.com
Biz-biotech owner address:   grunwald@netcom.com
Posting address:             biz-biotech@netcom.com

* How to SUBSCRIBE to biz-biotech:

    Send the following message
        SUBSCRIBE BIZ-BIOTECH
    to
        LISTSERV@NETCOM.COM

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    Send the following message
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One of the major goals the of BIZ-BIOTECH list is to bring together 
scientist and business professionals from industry and academia that work 
on 
biomedical and biotechnical projects. To share information on ideas, 
projects, resources etc., creating a world-wide information and 
communication-forum around BIOTECHNOLOGY and its business applications 
and opportunities.

Topics of BIZ-BIOTECH include discussion about:
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Subscribers to this list may be researcher from academia, industry 
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the topic of biotechnology and its commercial possibilities. Subscribers 
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Personal messages and personal replies should be sent to an individual 
and NOT to the whole list. Otherwise, it might annoy other colleague 
BIZ-BIOTECH subscribers. Obviously, replies of general interest should be 
send to the entire list.

BIZ-BIOTECH is currently an open, unmoderated list. Any postings are 
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grunwald@netcom.com). Subscribers are encouraged to support BIZ-BIOTECH 
actively within their own organizations and personal or professional 
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For further information about BIZ-BIOTECH, please send an e-mail to

Stefan Gruenwald
(grunwald@netcom.com)
BIZ-BIOTECH listowner



From marb1534@mailszrz.zrz.tu-berlin.de Wed Mar  1 09:54:05 EST 1995
Article: 1524 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!howland.reston.ans.net!Germany.EU.net!netmbx.de!zrz.TU-Berlin.DE!q610.lb.tu-berlin.de!user
From: marb1534@mailszrz.zrz.tu-berlin.de (Karin Marbach)
Newsgroups: bionet.mycology
Subject: mito membrane proteinfractions
Date: 24 Feb 1995 07:58:32 GMT
Organization: Mikrobiologie und Genetik
Lines: 16
Message-ID: <marb1534-2402950858090001@q610.lb.tu-berlin.de>
NNTP-Posting-Host: q610.lb.tu-berlin.de

I want to prepare a pure protein fraction of the inner mitochondrial
membrane of the fungus I am working with, to see if I can get my protein
to show up in this membrane. 

Does someone know a method to end up with fractions of proteins of the
outer membrane, the inner membrane and the matrix of mitochondrien? I have
heard that there exists something like that once.

I will be thankfull for references too.

Please contact me over Email if you can help me out.
Thanks!


Elian van Nunen, Technical <university Berlin
Email: nune1534@mailszrz.zrz.TU-Berlin.DE


From eskin@nak.berkeley.edu Wed Mar  1 09:54:06 EST 1995
Article: 1525 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!howland.reston.ans.net!agate!edster.berkeley.edu!user
From: eskin@nak.berkeley.edu (Edster)
Newsgroups: bionet.biology.tropical,bionet.molbio.bio-matrix,bionet.mycology,bionet.plants,misc.health.alternative
Subject: Re: Database of Plants & Their Uses
Date: Sun, 26 Feb 1995 18:33:43 -0700
Organization: Here
Lines: 5
Message-ID: <eskin-2602951833430001@edster.berkeley.edu>
References: <3igc8h$7fa$1@mhade.production.compuserve.com>
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Xref: bigblue.oit.unc.edu bionet.biology.tropical:510 bionet.molbio.bio-matrix:502 bionet.mycology:1525 bionet.plants:5468 misc.health.alternative:23800

Tim,
   I am interested in your database but went to oak.oakland.edu and
couldn't find it.  Is the name of the program Herbage?  Is it there yet.  
Thanks,
Ed


From calmush1@aol.com Wed Mar  1 09:54:07 EST 1995
Article: 1526 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!swrinde!cs.utexas.edu!uunet!in1.uu.net!newstf01.news.aol.com!newsbf02.news.aol.com!not-for-mail
From: calmush1@aol.com (CAL MUSH 1)
Newsgroups: bionet.mycology
Subject: MORAL'S
Date: 26 Feb 1995 19:24:23 -0500
Organization: America Online, Inc. (1-800-827-6364)
Lines: 8
Sender: root@newsbf02.news.aol.com
Message-ID: <3ir63n$sj9@newsbf02.news.aol.com>
Reply-To: calmush1@aol.com (CAL MUSH 1)
NNTP-Posting-Host: newsbf02.mail.aol.com

WoW.  Just found about a 40+ acre cherry orchard that is just booming with
yellow Moral's.  I filled my kind's easter basket in about a 100 yard
walk.
What the best way to identify the edibility of these mushroom's.  Don't
want to eat or sell any bad shrooms to anybody.

Thank's Ray
The more we know the more we grow.


From tprice@tulsa.com Wed Mar  1 09:54:09 EST 1995
Article: 1527 of bionet.mycology
Path: bigblue.oit.unc.edu!concert!gatech!swrinde!news.uh.edu!news.sccsi.com!nuchat!usenet
From: tprice@tulsa.com (James T. Price)
Newsgroups: bionet.mycology
Subject: Re: Oxalic acid and Agaricus
Date: 26 Feb 1995 16:35:30 GMT
Organization: Your Organization
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References: <jmicales.150.2F4E3100@facstaff.wisc.edu>
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	Some human pathogenic Aspergilli can be suspected in tissue by the 
large number of oxalic crystals seen microsco