Table 1. Migration Rate Table Oligonucleotide size Polyacrylamide concentration (%) ______________________________________________________________________ <25 20 25-50 15 50-90 12 >90 8
Table 2. Recipes for Polyacrylamide Gels
Gel Thickness Total vol. %gel 40%acryl. Urea 10XTBE APS TEMED (mm) (mL) (%) (mL) (g) (mL) (mL) (µL) ________________________________________________________________________ 0.4 100 8 20 42 10 4.4 60 12 30 42 10 4.4 60 20 50 42 10 4.4 60 _________________________________________________________________________ 0.8 150 8 30 63 15 6.6 100 12 45 63 15 6.6 100 20 50 63 15 6.6 100 _________________________________________________________________________ 1.6 250 8 50 105 25 11.0 150 12 75 105 25 11.0 150 20 125 105 25 11.0 150 _________________________________________________________________________ Recipes are for 30 x 40cm gels.