Table 2. Recipes for Polyacrylamide Gels
Gel Thickness Total vol. %gel 40%acryl. Urea 10XTBE APS TEMED
(mm) (mL) (%) (mL) (g) (mL) (mL) (µL)
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0.4 100 8 20 42 10 4.4 60
12 30 42 10 4.4 60
20 50 42 10 4.4 60
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0.8 150 8 30 63 15 6.6 100
12 45 63 15 6.6 100
20 50 63 15 6.6 100
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1.6 250 8 50 105 25 11.0 150
12 75 105 25 11.0 150
20 125 105 25 11.0 150
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Recipes are for 30 x 40cm gels.
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