Subject: GUS staining

From: [email-masked] (Qiudeng Que)
Date: 2 Aug 1995 17:25:08 GMT

Dear Netter; 

I am trying to do some GUS staining of Petunia corollas. Usually the tube
stains well, but the limb is barely stained. The problem is probably
caused by poor penetration of X-gluc due to the presence of cuticle layers
on the surface. Could someone suggest a protocol for that? I did vacuum
infiltration briefly, it did not work well. 

Thanks. 

Qiudeng Que 
Department of Environmental Horticulture
Univ.of California
Davis, CA 95616
[email-masked]