Subject: isolation of nucleus of seed cells

From: [email-masked] (consuelo medeiros rodrigues de lima)
Date: 26 Jul 1995 15:12:35 -0700

dear netters
I am working with a leguminous seed protein that seems to occur at 
the nucleus and cytosol. In order to confirm the nuclear localization
I need a very pure nuclear fraction. So I would like very much if 
someone could send me a protocol or references concerning nucleus
purification of seed cells.