Subject: Synchronizing ES cells

From: [email-masked] (K Lee)
Date: Sun, 19 Sep 1993 10:51:14 GMT

Can anyone give me advice on synchronizing embryonic stem (ES) cells?  Thymidine
and hydroxyurea aren't very effective.  I've also tried doing a reversible
colcemid block followed by a mitotic shake off but got a pathetic yield
of mitotic cells.  Thanks in advance.

Kenneth Lee
AFRC Center for Genome Research
West Mains Road
Edinburgh EH9 3JQ

(E-mail:[email-masked])