Subject: subcellular localization of DNAses

From: [email-masked] (Robert Kuhelj, IJS)
Date: 2 Dec 93 14:31:16 +0100

Hi there,

has anyone got a clue about subcellular localization of mammalian DNAses, i.e.
which organelles contain these enzymes. Are any DNAses to be found in nucleus
as well?  What about the cytosol?

If one freezes a tissue sample and afterwards lets it thaw overnight, the
genomic DNA will be degraded. Are only lysosomal DNAses (lysosomes break
during freezing & thawing) responsible?

Thanks, Robert