Subject: (none)

From: [email-masked]
Date: 18 Jun 1996 10:47:41 -0700

Aspergillus nidulans Chromosome II - Version 2 - Aligned with Genetic Map - 105 Probe Pools
                                                                                                            MINIMAL MAP
                                                                                                        Estimated Coverage = 80%
                                                                                                        Estimated Physical Size = 3350 kb
KB            LOCUS     CLONE/CHR         CLONE                10        20         30        40        50         60        70        80        90        100        
                        HYBR.             NAME         .........|.........|.....|....|.........|.........|...|......|.........|.........|.........|...|......|.....
                        12345678          ---------------------------------------------------------------------------------------------------------------------------
|-0                      L              * SW14BO2      1........................|............................|........................................|............
|             cysB       H                SL21B1O      11.......................|............................|........................................|............
|-50                     H              * SLO5F11      .1.......................|............................|........................................|............
|                        MMH  MH          RW12H11      .11..1.....1....111......|............................|...................1..............1.....|..1.........
|-100                    M              * SW28BO9      ..1......................|............................|........................................|............
|             cen       HMHHHMMH          OWO2CO4      .111.1........11.........|............................|........................................|......1.....
|-150                    M              * SW23BO6      ...1.....................|............................|........................................|............
|                        M                SWO7CO4      ...111...................|............................|........................................|............
|                        M              * SW22EO4      ....11...................|............................|........................................|............
|-200         CEN        M              * SWO7H11      .....1...................|............................|........................................|............
|             cen       HHHHHHMH          OW19HO2      .....11..................|............................|........................................|............
|-250                    M              * SW23HO9      ......1..................|............................|........................................|............
|                        HHHHMML          RL15CO2      ......11.................|............................|........................................|............
|-300                    M              * SW23CO1      .......1.................|............................|........................................|............
|                       MHHMH M           RWO9C12      ......111................|............................|........................................|............
|-350                    M              * SW14E12      ........1................|............................|........................................|............
|                        H                SLO5GO4      ........11...............|............................|........................................|............
|-400                    M              * SW11FO1      .........1...............|............................|........................................|............
|                        L     L          RL15B11      .........11..............|............................|........................................|............
|-450                    M              * SW3OGO6      ..........1..............|............................|........................................|............
|                        MHMLHMM          RW12F1O      ..........11.............|............................|....1...................................|............
|-500                    M              * SW21GO7      ...........1.............|............................|........................................|............
|                        HMMHHMM          RL29GO8      ...........111...........|............................|....................................1...|............
|-550                    M              * SW3OGO8      ............1............|............................|........................................|............
|                        HMHHHL           RWO5FO8      ............11...........|............................|........................................|............
|                        M              * SW23G12      .............1...........|............................|........................................|............
|                        H              * SLO7HO4      .............11..........|............................|........................................|............
|-600                    M              * SW1OF1O      ..............11.........|............................|........................................|............
|                        MLH  MM          RWO2BO2      .111.11.......111........|.........................1..|......................................1.|.......11...
|-650                    L              * SL21FO8      ................1........|............................|........................................|............
|                        L     L          RW17HO8      ................11.......|................1...........|...........11........1..................|1......1....
|-700                    L              * SW18BO4      .................1.......|............................|........................................|............
|             cen       MHMMMHHM          OW12A11      ..1........111...11......|.......1....................|...........1......1.1...................|.......1....
|                       LM  MM L          RL16C12      ..................1......|............................|........................................|............
|                        M    MH          RW13F11      ..................11.....|............1...............|...1............11.......11.............|............
|-750                    H              * SLO7CO2      ...................111...|............................|........................................|............
|                        H                SL18CO3      ...................111...|............................|........................................|............
|                        M              * SL12CO7      ....................11...|............................|........................................|............
|                        H              * SL31DO6      .....................1...|............................|........................................|............
|-800                    H              * SL28G12      .....................11..|............................|........................................|............
|                        M M  LH          RW21AO4-d    1....1.1......11......111|......1.............111..1..|11...11....1111............111...11.111.|1....111111.
|-850                    M              * SWO3C12      .........1.............1.|............................|........................................|............
|                        M M  LH          RW21AO4      1....1.1......11......111|......1.............111..1..|11...11....1111............111...11.111.|1....111111.
|-900                    L  L             RWO4G11      ........................1|............................|........................................|............
                                                       ------------------------------------------------------------------------------------------------------------
|-0                      H              * SL21GO4      .........................|11..........................|........................................|............
|                        M              * SW31CO9      .........................|.1..........................|........................................|............
|                        HM               RL26EO9      ..1......................|.11.1..1....................|.....................................1..|............
|-50                     H              * SL14FO2      .........................|..1.........................|........................................|............
|                        M  H             RLO9HO1      .........................|.111........................|........................................|............
|-100                    H              * SW3OHO3      .........................|...1........................|........................................|............
|                        M                SL11C11      .........................|...11.......................|........................................|............
|-150                    L              * SW12CO1      .........................|....1.......................|........................................|............
|                        MLM              RL12AO2      .........................|....11.....1................|........................................|......1.....
|-200                    L              * SWO9D1O      .........................|.....1......................|........................................|............
|                        M     H          RLO9BO5      .........................|.....11.....................|........................................|............
|-250                    M              * SW11CO2      .........................|......1.....................|........................................|............
|                        H                SL12BO5      .........................|......11....................|........................................|............
|                        H              * SL13AO3      .........................|.......11...................|........................................|............
|-300                    H              * SL13H1O      .........................|.......11...................|........................................|............
|                       HM    M           RLO9FO7      .........................|........11..................|........................................|............
|-350                    M              * SWO7HO4      .........................|.........1..................|........................................|............
|                        M   H            RL21BO8      ..1......................|.........111................|........................................|............
|                        M              * SWO4A12      .........................|..........1.................|........................................|............
|-400                    H              * SL22DO7      .....................1...|..........11................|........................................|............
|                       MHHLH L           RW13FO5      .........................|1..........11...............|........................................|............
|-450                    H              * SL2OAO3      .........................|............1...............|.......................1................|............
|                       MLHMHL            RWO2F1O      .....1...................|............11............1.|........................................|............
|-500                    M              * SL15CO4      .........................|.............1..............|........................................|............
|                        M                SWO1F11      .........................|.....1.....1.11.............|.......................1................|............
|                        L                SLO8BO2      ....................1....|..............111...11......|........................................|............
|                        H              * SL3OF12      .........................|...............1............|........................................|............
|-550                    H              * SW21GO2      .........................|...............11...1.......|........................................|............
|                        H                SL23AO9      .........................|................11..........|........................................|............
|                        H              * SL25BO2      .........................|.................11.1.......|........................................|............
|-600                    L              * SW31E1O      .........................|..................1.........|........................................|............
|                        M                SLO4AO5      .........................|.................111........|........................................|............
|                        L              * SW23HO6      .........................|...................11.......|........................................|............
|                        H              * SL12DO1      .........................|....................11......|........................................|............
|                        M              * SL27EO7      .........................|.....................1......|........................................|............
|-650                    M              * SL23CO8      .........................|.....................11.....|........................................|............
|                        L                SW15EO3      ..1.......1..............|......111....11......111....|............111....11...1...11.1...1111.|.....111....
|-700                    M              * SWO5D12      .........................|.......................1....|........................................|............
|                        M                SW29CO3      ..............1..........|.............1........111...|..1.......1............1............1...|............
|-750                    H              * SL26DO1      .........................|........................1...|........................................|............
|                        M                SW27HO9      .......1.................|........................11..|........................................|............
|                        M              * SL25A12      .........................|.........................1..|........................................|............
|-800                    M              * SW17FO8      .........................|.........................11.|........................................|............
|                       MH    L           RW1OEO7      .........................|............1.............11|........................................|............
|-850                    L              * SWO6GO7      .........................|...........................1|........................................|............
                                                       ------------------------------------------------------------------------------------------------------------
|-0                      L              * SW3OD12      .........................|............................|1.......................................|............
|                        L                SW28B12      .........................|............................|11......................................|............
|-50                     H              * SW27H12      .........................|............................|.1......................................|............
|                        M                SWO8GO5      .........................|............................|.111....................................|............
|                        M              * SWO9DO6      .........................|............................|..1.....................................|............
|-100                    H              * SLO5EO9      .........................|............................|.111....................................|............
|                        M                SW1ODO6      .........................|............................|..11....................................|............
|                        L                SW3OG11      .........................|............................|...11...................................|............
|-150                    H              * SLO8EO7      .........................|............................|....1...................................|............
|                        HLL  MM          RW27DO1      .........................|............................|....11..................................|............
|-200                    L              * SWO9E12      .........................|............................|.....11.................................|............
|                       HM                RLO8H1O      .........................|............................|......11................................|............
|-250         (nimE)     M              * SW21E1O      .........................|............................|.......1................................|............
|                        M  H             RL27HO9      .........................|............................|.......11...............................|............
|-300                    M              * SL25BO7      .........................|............................|........1...............................|............
|                        LH H             RW15HO4      .........................|1.......1...................|........11..............................|............
|                        L              * SW12FO4      ....................1....|............................|.........11.............................|............
|                        H              * SL17BO4      .........................|............................|..........11............................|............
|-350                    M              * SW12B11      .........................|............................|..........11............................|............
|                        L                SWO1DO3      .........................|............1...............|..1........11...........................|.........1..
|-400                    H              * SLO4GO9      .........................|..............1.............|............1...........................|............
|                        M    L           RWO8DO5      .............111..1......|...........1................|............11..........................|............
|-450                    H              * SL19DO7      .........................|............................|.............1..........................|............
|                       ML                RWO7HO7      ....1....................|............................|.............11.........................|............
|                        H              * SL18DO2      .........................|............................|..............1.........................|............
|-500                    M              * SL17G11      .........................|............................|..............11........................|............
|                       LH                RLO6G12      ...........1............1|..............1.............|..............111......1................|............
|-550                    H              * SLO8GO4      .........................|............................|..............1.1.......................|............
|                       MMH MMLM          RW13A1O      .........................|...........11...............|........1......111......................|............
|                        H              * SL13EO1      .........................|............................|.................111....................|............
|                        M              * SW1OEO1      .........................|............................|..................11....................|............
|-600                    H              * SL2OAO1      .........................|............................|...................1....................|............
|                        MLMHHLH          RL19AO6      ....11...................|............................|...................11...................|............
|-650                    M              * SL16D1O      .........................|............................|....................1...................|............
|                       MLMMHMMM          OL18A11      .....1...................|............................|....................11..................|............
|                        H    H           RL28H1O      .........................|............................|.....................1..................|............
|                       LML HL M          RW26AO8      ....11...................|............................|....................111.................|............
|-700                    H              * SL32FO9      .........................|............................|......................1................1|............
|                        H                SL11B1O      .........................|............................|......................11................|............
|-750                    M              * SL15EO3      .........................|............................|.......................1................|............
|                        M                SW21C12      .........................|............................|.......................111..............|............
|-800                    H              * SL31EO8      .........................|............................|........................1...............|............
|                        H                SLO6B1O      .........................|............................|........................11..............|............
|                        H              * SLO3C12      ..1......................|............................|.........................11.............|............
|                        M              * SL11DO8      .........................|............................|..................1.......11............|............
|                        M              * SW16BO7      .........................|............................|...........................11...........|............
|                        L              * SW16A12      .........................|............................|............................1.1.........|............
|-850                    H              * SLO6A11      .........................|............................|............................1...........|............
|                        L                SW21HO5      .........................|............................|............................1111........|............
|-900                    H              * SL29FO6      .........................|............................|.............................111........|............
|                        M                SL29FO8      .........................|............................|.............................1111.......|............
|-1000   (adC,adD,dewA)  M              * SW18BO1      .........................|............................|................................1.......|............
|             cen       HMHHHMMH          OW14AO2      .111.11........111.......|............................|............1...................111.....|............
|-1050                   L              * SW29BO9      .........................|............................|.................................1......|............
|                        LMMH             RW18CO2      ......1.............1....|....................11....1.|.........11.......1.............111...1.|.....1......
|-1100                   H              * SL15AO8      .........................|............................|..................................1.....|............
|                        M                SWO7G12      .........................|............................|.....................1............11....|............
|-1150                   H              * SLO5CO4      .........................|............................|...................................1....|............
|                        HMMMHM           RL11BO3      .........................|............................|...................1...............11...|............
|                        M              * SL27DO7      ................1........|............................|....................................11..|............
|-1200                   H              * SL19A1O      ................1........|............................|....................................11..|............
|                        M                SLO8DO2      .........................|............................|....................................111.|............
|-1250                   M              * SWO9BO4      .........................|............................|....................................1.1.|............
|                        H                SL1OA11      .........................|............................|......................................11|............
|-1300                   M              * SW22GO5      .........................|............................|.......................................1|............
                                                       ------------------------------------------------------------------------------------------------------------
|-0                      HLH HMM          RW19G12      ............1............|............1...............|...........................1.......11.1.|11......1...
|                        L     L          RL24DO9      ...1.....................|.......11...................|........................................|.11.1.......
|                        M                SW15FO9      .........................|............................|......................................1.|..1.........
|                        L  L             RW27GO1      .........................|............................|........................................|..11........
|-50                     H              * SLO5DO9      .........................|............................|............1...........................|...1........
|                        MMH   L          RWO6FO4      .........................|............................|.................1......................|...11.......
|                        H              * SL13EO9      .........................|............................|........................................|....1.......
|-100                    M              * SW24DO8      .........................|............................|........................................|....11......
|                       MLHM   M          RW22GO1      .........................|............................|........................................|.....11.....
|-150                    H              * SL26EO4      .........................|............................|....1...................................|......1.....
|                        HLHMHL           RW16EO6      ...........1.1...1..1....|.....................1......|....1.......111.........1...1111....1...|......111...
|                        M              * SW13FO2      .........................|............................|........................................|.......1....
|-200                    M              * SL23EO9      .........................|............................|........................................|.......11...
|                        M                SL32DO2      .........................|............................|........................................|........11..
|-250                    M              * SWO8F12      .........................|............................|........................................|.........1..
|                        M    HH          RW16CO5      .........................|............................|........................................|.........11.
|                        H              * SWO9G11      .........................|............................|........................................|..........1.
|-300                    M              * SWO5D1O      .........................|............................|........................................|..........11
|                        H                SWO4GO3      .........................|............................|...................................1....|...........1
                                                       ------------------------------------------------------------------------------------------------------------

In the first column a scale in kilobases (kb) is provided for the chromosome walk.  The chromosome walk above is aligned with the genetic map. 

In the second column Genetic loci assigned to cosmids are found.  Cosmids, which hybridize to another complementing
clone or to a clone, which hybridizes to a gene probe, are given in parantheses.  The parantheses
around the locus name implies that the cosmid listed by name in the same row has not been
shown to complement directly a mutation at the genetic locus or hybridize directly to a gene probe.
CEN denotes the predicted location of the centromere, while cen in lowercase denotes a centromeric repeat.

In the third column hybridization of the clone to each chromosome is scored as high (H), medium (M), or low (L).

In the fourth column the star (*) denotes that the cosmid was used as a probe.

In the fifth column is the clone name.

NOTES ON THE PHYSICAL MAP:

There is considerable uncertainty about the genetic map position of dewA, and so we have resolved
the conflict in favor of its position on the physical map.