Subject: re: calcium in fungi

From: [email-masked] (Nick Read)
Date: 17 Feb 1995 11:31:41 -0800

In our experience, Fura-2 cannot be ester-loaded into 
Neurospora hyphae.  If it is microinjected as its free 
acid, it is rapidly sequestered within organelles 
making measurements of cytosolic free calcium 
impossible.  We have documented the problems of using 
calcium dyes with fungi in some detail 
in Knight et al. (1993).  Mycol. Res. 97: 1505 and in 
Read et al. (1992).  J. Microsc. 166: 57.

We haven't used ionomycin but have had success with 
bromo-A23187 (see Knight et al., 1993).

Nick Read