Subject: (none)

From: [email-masked]
Date: 18 Jun 1996 10:47:42 -0700

Aspergillus nidulans Chromosome III - Version 2 - Aligned with Genetic Map - 109 Probe Pools
                                                                                                            MINIMAL MAP
                                                                                                        Estimated Coverage = 100%
                                                                                                        Estimated Physical Size = 3600 kb
KB            LOCUS     CLONE/CHROM       CLONE                10        20        30         40        50         60        70        80         90        100        
                        HYBR.             NAME         .........|.........|.........|.|........|.........|........|.|.........|.........|...|......|.....|....|..|.......
                        12345678                       ------------------------------------------------------------------------------------------------------------------
|-0                       M             * SW28CO7      1..............................|...........................|.........................|............|.......|.......
|                        HHMH L           RWO8CO9      11.............................|...........................|.........................|............|.......|.......
|                         H             * SW29DO2      .11............................|...........................|.........................|............|.......|.......
|-50                      L             * SW19FO6      .11............................|...........................|.........................|............|.......|.......
|                         L   M           RL3OHO1      ..11.........................1.|...........................|.........................|............|.......|.......
|                         M             * SL25DO3      ...11..........................|...........................|.........................|............|.......|.......
|-100                     L             * SW22B1O      .1.11..................1.......|...........................|.........................|............|.......|.......
|                       L L  M            RWO2DO2      ....11.........................|...........................|.........................|............|.......|.......
|-150                     L             * SW3OA11      .....1.........................|.............1.............|......1..................|............|.......|.......
|                         M               SLO8HO9      .....11........................|...........................|.........................|............|.......|.......
|                         H             * SWO8BO8      ......111......................|...........................|.........................|............|.......|.......
|                         H             * SLO9H12      .......1.......................|...........................|.........................|............|.......|.......
|-200                     L             * SL13HO3      ......111......................|...........................|.........................|............|.......|.......
|                         M  MHL          RW14GO6      ........11.....................|...........................|.........................|............|.......|...1...
|-250         samB        M             * SL19BO5      .......1.1.....................|...........................|.........................|............|.......|.......
|                       HMMMML M          RW19HO5      .........11....................|.......................1...|.........................|............|.......|.......
|-300                     M             * SW25GO9      ..........1....................|...........................|.........................|............|.......|.......
|                         M MH            RL29AO2      ..........11...................|...........................|.........................|............|.......|.......
|-350                     H             * SLO7G12      ...........1...................|...........................|.........................|............|.......|.......
|                         M               SL11EO7      ...........11..................|...........................|.........................|............|.......|.......
|-400                     M             * SL28AO5      ............1..................|...........................|.........................|............|.......|.......
|                         M  H            RW3OHO5      ........1...11.................|...........................|.........................|............|.......|.......
|                         MM H L          RW26CO6      .............1.................|...........................|.........................|............|.......|.......
|                         LM              RLO7GO7      .............11................|...........................|.........................|............|.......|.......
|-450                     M             * SL11DO2      ..............1..1.............|...........................|.........................|............|.......|.......
|                         H H L           RL14AO4      ..............111..............|..................1........|.........................|...1........|.....1.|.......
|                         H             * SL27BO1      ...............1...............|...........................|.........................|............|.......|.......
|-500                     M             * SLO8D12      ...............11..............|...........................|.........................|............|.......|.......
|                        LHH H            RL32AO2      ................11.............|...........................|.........................|.........1..|.......|.......
|                         H             * SLO3D1O      ........1........11............|...........................|.........................|............|.......|.......
|-550                     M             * SL25DO5      ..................1............|...........................|.........................|............|.......|.......
|                         LLH             RW19FO2      ...........1.....1111.........1|...........................|1........................|............|1......|.......
|                       HLL H             RW18HO7      ..................111..........|...........................|1........1...............|............|.......|.......
|-600                     M             * SLO5BO1      ....................1..........|...........................|.........................|............|.......|.......
|                         L               SWO9BO6      ....................111........|...........................|.........................|............|.......|.......
|                         M             * SL24F12      .....................1.........|...........................|.........................|............|.......|.......
|-650                     H             * SW23D12      .....................11........|...........................|.........................|............|.......|.......
|                        HMHHHL           RWO5FO8      ......................11.......|...........................|.........................|............|.......|.......
|                         M             * SL22C1O      .......................1.......|...........................|.........................|............|.......|.......
|-700                     M             * SLO7AO1      .......................11......|...........................|.........................|............|.......|.......
|                         LM              RW24FO4      ........................11.....|...........................|.........................|............|.......|.......
|                         H             * SWO1AO6      .........................11....|...........................|.........................|............|.......|.......
|                         M             * SW26FO4      ..........................11...|...........................|.........................|............|.......|.......
|-750                     M             * SL3OE11      ...........................1...|...........................|.........................|............|.......|.......
|                         M               SLO9BO2      ...........................11..|...........................|.11......................|............|.......|.......
|-800                     M             * SLO7FO2      ............................1..|...........................|.........................|............|.......|.......
|                       H L   L           RWO3AO8      ............................11.|...........................|.........................|............|.......|1.....1
|-850                     M             * SL32AO5      .............................1.|...........................|.........................|............|.......|.......
|                         M               SL22E11      ...........................1.11|...........................|.........................|............|.......|.......
|-900                     M             * SLO6A12      ..............................1|...........................|.........................|............|.......|.......
                                                       ------------------------------------------------------------------------------------------------------------------
|-0                       M             * SL21CO4      ...............................|1..........................|.........................|............|.......|.......
|             cen       MHMMMHHM          OW12A11      ..................1...11.......|11...1.............1....1..|..........1.......1......|............|.....1.|.......
|-50     (phytochrome)    M             * SW19GO3      ............................1..|.1.........................|.........................|............|.......|.......
|                         L H             RL14HO6      ...............................|.11........................|.........................|............|.......|.......
|-100                     M             * SW19DO5      ...............................|..1........................|.........................|............|.......|.......
|                         L               SW1OA1O      ...............................|..11.......................|.........................|............|.......|.......
|-150                     L             * SWO5CO6      .....................1.........|...1.......................|.........................|1...........|.......|.......
|                         H    M          RW18AO9      ...............................|1..111.........1...........|........................1|..1....11...|.......|....1..
|-200                     M             * SL24F1O      ...............................|.....1.....................|.........................|............|.......|.......
|                         M               SW27HO4      ...............................|.....11....................|.........................|............|.......|.......
|-250                     H             * SWO5CO2      .......................1.......|......1....................|.........................|............|.......|.......
|                         LHHHLL          RWO2CO6      ...............................|......11...............1...|.........................|............|.......|.......
|                         M             * SL25GO2      ...............................|.......11..................|.........................|............|.......|.......
|-300                     M             * SL26F1O      ...............................|........1..................|.........................|............|.......|.......
|                         LL              RL12EO4      ...............................|........11.................|.........................|............|.......|.......
|-350                     M             * SW18H12      ...............................|.........1.................|.........................|............|.......|.......
|                        HL   M           RL11GO3      ...............................|.........11................|.........................|............|.......|.......
|-400                     L             * SL24GO2      ...............................|..........1................|.........................|............|.......|.......
|                        MM               RW29CO5      ...............................|..........11...............|.........................|............|.......|.......
|             cen       HHHMHMLH          OWO1GO4      .......1.......................|...........11..........1...|.........................|............|.......|.......
|-450                     L             * SL2OH11      ...............................|............1..............|.........................|............|.......|.......
|                         LM M            RW3OEO4      ...............................|............11.............|.........................|..........1.|.....1.|.......
|-500                     M             * SLO7BO6      ...............................|.............1.............|.........................|............|.......|.......
|                         L               SW14AO1      ...............................|.............11............|.........................|............|.......|.......
|-550                     M             * SWO2DO3      ...............................|..............1............|.........................|............|.......|.......
|             cen       HMHHHMMH          OW14AO2      1..1................1..........|11............111...1..1...|........................1|............|11.....|.......
|-600                     M             * SW25HO1      ...............................|...............1...........|.........................|............|.......|.......
|             samB        M   HM          RL31GO8      ........1.....1..1...1..1..11..|..1...1........11..........|......1...1.........11...|............|1......|..1....
|                         LLMM L          RWO7AO2      .........1.....................|................1..........|.........................|............|.......|.......
|                        LMM M L          RW23CO9      ...............................|................111........|...............11........|............|.......|.......
|-650                     M             * SL16C1O      ...............................|.................1.........|.........................|............|.......|.......
|                        HMM MMM          RW23B12      ...............................|................111........|.........................|............|.......|.......
|-700                     H             * SLO5G11      ...............................|..................1........|.........................|............|.......|.......
|                        MLMHM            RL32HO8      1..............................|..................11.......|..............1..........|............|..1....|.......
|-750                     H             * SW29EO9      ...............................|...................1.......|.........................|............|.......|.......
|                         M               SL1OEO4      ...............................|...................11......|.........................|............|.......|.......
|-800                     M             * SL25HO5      ...............................|....................1......|.........................|............|.......|.......
|                         H               SL1OAO4      ...............................|....................11.....|.........................|............|.......|....1..
|                         M             * SLO8CO2      ...............................|.....................11....|.........................|............|.......|.......
|-850                     M             * SW24H12      ...............................|......................1....|.........................|............|.......|.......
|                         LM  LM          RW22GO9      .....1..............1..........|1.....................111..|...............1111.....1|1.....1.1...|1....11|.......
|-900         CEN         M             * SLO9DO9      ...............................|.......................1...|.........................|............|.......|.......
|                       MLMMHMMM          OL18A11      ...............................|.......................11..|.........................|............|.......|1......
|                         L             * SLO7AO7      ...............................|........................11.|.........................|............|.......|.......
|                         H             * SW21HO7      ...............................|.............1...........1.|.........................|............|.......|.......
|-950                     M             * SL24EO7      ..1...........................1|.........................11|.......1.................|............|....1..|.......
|                        LL               RW15GO3      ...............................|..........................1|.........................|............|.......|.......
                                                       ------------------------------------------------------------------------------------------------------------------
|-0                       M             * SWO5G1O      ...............................|...........................|1........................|............|.......|.......
|             rodA        H               SW21HO6      ...............................|...........................|11.......................|............|.......|.......
|-50                      L             * SW21A12      ...............................|...........................|.1..................1....|............|.......|.......
|                        LMMH             RW18CO2      1...........1..................|.....11.......111.........1|.11............1.........|............|.......|....11.
|-100                     L             * SL15BO6      ...............................|...........................|..1......................|............|.......|.......
|                         M   M           RL28E1O      ...............................|...........................|..111....................|............|.......|.......
|      (amdSI,bimB)       M             * SWO4EO6      ...............................|...........................|...11....................|............|.......|.......
|-150                     M             * SW29A12      ...............................|...........................|....1....................|............|.......|.......
|                       MLM               RW18EO4      ..........................1....|...........................|....11...................|............|.......|.......
|-200                     M             * SLO4EO2      ...............................|...........................|.....1...................|............|.......|.......
|                         H               SW1OAO8      ...............................|...........................|.....1111................|............|.......|.......
|                         M             * SL18CO4      ...............................|...........................|......11.................|............|.......|.......
|-250                     M             * SL23D11      ...............................|...........................|......11.................|............|.......|.......
|                         M               SWO8EO8      ...............................|...........................|......111................|............|.......|.......
|-300                     M             * SL21H11      ...............................|...........................|.......11................|............|.......|.......
|                         MH M            RWO2E1O      .................1......1...11.|...........................|........11...............|............|.......|.......
|-350                     H             * SL2OGO7      ...............................|...........................|.........1.............11|............|.......|.......
|                         L               SW13AO9      ........1.......1..............|...........................|........11...........1...|............|.......|.......
|                         H               SW19F12      .......1.......................|.................1.........|.........11.............1|............|.......|.......
|-400                     L             * SL16EO2      ...............................|...........................|..........1..............|............|.......|.......
|                         LL M            RW3OAO7      ...............................|...........................|..........11.............|............|.......|.......
|-450                     H             * SWO8DO6      ...............................|...........................|...........1.............|............|.......|.......
|                         M               SL23BO2      .....................1.........|...........................|..1........11.....1......|..1...1.1...|..1....|.......
|                         L             * SW14EO4      ...............................|...........................|............1............|............|.......|.......
|                         H             * SLO7EO1      ...............................|...........................|............111..........|...........1|.......|.......
|                         M             * SW25GO6      ...............................|...........................|.............1...........|............|.......|.......
|-500                     M             * SL24BO2      ...............................|...........................|.............1...........|............|.......|.......
|                         M               SL22FO4      ...............................|...........................|.............11..........|............|.......|.......
|                        LHL              RW31B12      .............................1.|..................11...1...|..............11.........|.1.......1..|.......|.......
|-550                     M             * SL2OE11      ...............................|...........................|...............1.........|............|.......|.......
|                         H               SWO2DO9      ...............................|...........................|...............11........|............|.......|.......
|                         M             * SL12CO3      ...............................|...........................|................1........|............|.......|.......
|-600                     H             * SW13DO1      ...............................|...........................|................11.......|............|.......|.......
|                         M               SL1OHO6      ...............................|...........................|...............1111......|............|.......|.......
|                         M             * SW14A12      ...............................|...........................|..................1......|............|.......|.......
|-650                     M             * SW18HO3      ...............................|...........................|..................11.....|............|..11...|.......
|                       MLMLMM L          RW23FO1      ...............................|...........................|...................11....|............|.......|.......
|-700                     M             * SLO4F1O      ...............................|...........................|....................1....|............|.......|.......
|                       MMMHMM            RWO3BO8      ....1..........................|...........................|....1...............11...|............|.......|.......
|-750         nimA        H             * SLO4F11      ...............................|...........................|.....................1...|............|.......|.......
|                         M HM            RLO6HO8      ...............................|...........................|.....................11..|............|.......|.......
|-800                     M             * SL21EO9      ...............................|...........................|......................1..|............|.......|.......
|                         MH L            RLO5H1O      ...............................|.........1.................|.........1....1.......11.|............|.......|.......
|-850                     M             * SW28C1O      ..........1....................|...........................|.......................1.|............|.......|.......
|                         H               SW15DO4      ...............................|...........................|.......................1.|............|.......|.......
|                         M               SL19HO2      ...............................|...........................|.......................11|............|.......|.......
|-900                     L             * SL12GO7      ...............................|...........................|........................1|............|.......|.......
                                                       ------------------------------------------------------------------------------------------------------------------
|-0                     M M               RL28FO2      ...............................|...........................|.........................|1...........|.......|.......
|                         M               SW26GO7      ...............................|...........................|.........................|1111........|.......|.......
|-50                      L             * SW17GO6      ...............................|...........................|.........................|.11.........|.......|.......
|                        MH  L            RL22CO8      ...............................|...........................|.........................|..11........|.......|.......
|-100                     M             * SL23DO4      ............1..................|...........................|.........................|...1........|.......|.......
|                        MH  M            RLO9A12      ...............................|...........................|.........................|...11.....1.|.......|.......
|-150                     H             * SWO9CO4      ...............................|...........................|.........................|....1.......|.......|.......
|                         M               SW19DO8      ...............................|...........................|.........................|....111.....|.......|.......
|                         M             * SL2OAO8      ...............................|...........................|.........................|.....1......|.......|.......
|-200                     M             * SW23DO1      ...............................|...........................|.........................|.....11.....|.......|.......
|                         H               SW19D11      ...............................|...........................|.........................|......11....|.......|.......
|-250                     M             * SL28HO9      ...............................|...........................|.........................|.......1....|.......|.......
|                        MLH  MM          RWO2BO2      1..1...........................|.1..................1..1...|...............1.........|......111...|11...1.|.......
|                         M             * SWO6CO9      ...............................|...........................|.........................|........1...|.......|.......
|-300                     H             * SW14DO9      ...............................|...........................|.........................|........111.|.......|.......
|                         L               SWO9FO6      ...............................|...........................|.........................|.........11.|.......|.......
|-350                     L             * SW22CO1      ...............................|...........................|.........................|..........1.|.......|.......
|                       MHL M             RW22CO4      ...............................|...........................|.........................|..........11|.......|.......
|-400                     H             * SW11BO1      ...............................|...........................|.........................|...........1|.......|.......
|                         L H             RLO9GO3      ...............................|...........................|.........................|...........1|.......|.......
                                                       ------------------------------------------------------------------------------------------------------------------
|-0                       H             * SL25AO1      ...............................|...........................|.........................|............|1......|.......
|                         L H             RL18BO8      ...............................|...........1...............|.........................|............|11.....|.......
|                         MM   M          RL11GO8      ...............................|...........................|.........................|............|.11....|.......
|-50                      H             * SW1OHO7      ...............................|...........................|.........................|............|..1....|.......
|                         L               SW27GO8      ...1.................1.........|...1....1..............1...|....1...1.........111111.|..1.........|..111..|......1
|-100                     L             * SW27CO9      ...............................|...........................|.........................|............|...1...|.......
|                         L M             RW29CO1      .......................1.......|...........................|.........................|......1.....|...11..|.......
|-150                     L             * SW16B11      ...............................|...........................|.........................|.1..........|....1..|.......
|                         L    H          RW19DO6      ...............................|...........................|.........................|............|....11.|.......
|-200                     L             * SWO4BO7      ...............................|...........................|.........................|............|.....1.|.......
|                        HLHMHL           RW16EO6      ..1.......1....1....11.1...1...|1.1...............11.......|....................1..11|.1....1.....|....111|....11.
|             cen       LMLMLMLH          OW14AO4      ...............................|...........................|.........................|............|......1|.......
|-250                     M             * SL15HO8      ...............................|...........................|.........................|............|......1|.......
                                                       ------------------------------------------------------------------------------------------------------------------
|-0                       L             * SLO6GO3      ...............................|...........................|.........................|............|.......|1......
|                       LML HL M          RW26AO8      ...............................|.......1...............11..|.........................|............|.......|11.....
|-50                      M             * SWO3FO1      ...............................|...........................|.........................|............|.......|.1.....
|                         H               SW21CO2      ...............................|...........................|.........................|............|.......|.11....
|-100                     M             * SL21E11      ...............................|...........................|.........................|............|.......|..1....
|                        HM               RL26EO9      ...............................|.1.........................|....1............11......|............|.......|..11...
|                         M  H            RLO3AO5      ...............................|...........................|.........................|............|.......|...1...
|                        HH HLL           RW19GO7      ...............................|...........................|............1..1.........|............|.......|...11..
|-150         (areA)      L             * SW28G12      ...............................|...........................|.........................|............|.......|....1..
|                       L L   L           RW28FO5      ...............................|...........................|.........................|............|.......|....11.
|             acoD        M             * SL12HO9      ...............................|...........................|.........................|............|.1.....|.....1.
|                         M             * SW21HO8      ...............................|...........................|.........................|............|.......|.....11
|-200                     L             * SW23EO2      ...............................|...........................|.........................|............|.......|......1
                                                       ------------------------------------------------------------------------------------------------------------------

In the first column a scale in kilobases (kb) is provided for the chromosome walk.  The chromosome walk above is aligned with the genetic map. 

In the second column Genetic loci assigned to cosmids are found.  Cosmids, which hybridize to another complementing
clone or to a clone, which hybridizes to a gene probe, are given in parantheses.  The parantheses
around the locus name implies that the cosmid listed by name in the same row has not been
shown to complement directly a mutation at the genetic locus or hybridize directly to a gene probe.
CEN denotes the predicted location of the centromere, while cen in lowercase denotes a centromeric repeat.

In the third column hybridization of the clone to each chromosome is scored as high (H), medium (M), or low (L).

In the fourth column the star (*) denotes that the cosmid was used as a probe.

In the fifth column is the clone name.

NOTES ON THE PHYSICAL MAP:
       The original physical map had 2 contigs.  4 breaks were introduced at putative false joins to align the genetic and physical maps.
       Clone L24BO2 doesn't hybridize to itself.
       ablA:  One complementing clone for ablA was deleted from this map because it falls next to the other two complementing clones on VI.
       acoD:  The second to last acoD clone should be moved next to other two, and it was.  This makes the right arm a little long with respect to the genetic map.
       bimE:  The bimE clone was deleted from this map because it maps to another chromosome.
       phytochrome: lo6fo8 (3,6), l12co5 (8), l14b1o (3,5), l14co1 (3,5), l21a11 (4), l26b11 (8), l27go5 (6). Phytochrome may not be on III.
       riboB: The one clone W91CO1 for riboB was removed. The riboB gene is on VIII.
       samB:  The gene samB has two noncontiguous sets of clones that hybridize to the gene.