Subject: Novozyme

From: [email-masked] (Dan Ebbole)
Date: 14 Dec 93 14:15:36 GMT

We have been using Interspex supplied Novozyme with good results.  However,
it seems to be very active: our normal protocol is for around 60 min
treatment of germinated N. crassa conidia for protoplasting.  This batch
gives complete protoplasting within 20 min, a 60 min digest yields
protoplasts with low transformability.
------------------------
Dr. Daniel Ebbole
[email-masked]
Dept. of Plant Pathology and Microbiology
Texas A&M University
College Station, TX 77843-2132