Subject: Re: MITES in fungal cultures
From: [email-masked] (Matthew Sachs)
Date: 28 Apr 1994 09:46:35 -0700
Newsgroups: bionet.mycology

>We have recently encountered a mite infestation in an incubator used to
>grow fungi. Can anyone suddest how we get rid of the mites, and what can
>we do to make sure they don't come back. They're causing losts of
>problems with our cultures.
>
>Thanks
>
>Jacques Soddell
>[email-masked]
>Biotechnology Research Centre
>La Trobe University, Bendigo
>PO Box 199
>Bendigo, VIC, 3550
>AUSTRALIA

As part of his course on Neurospora Genetics at Stanford University, David
Perkins included a section on controlling mite infestations of fungal
cultures  He recommended procedures described in:

Subden, RE and Threlkeld, SFH (1966) Mite control for Neurospora labs.
Neurospora Newsl. 10: 14.

Their prescription:

To kill mites, freeze cultures infested with mites at temperatures below
-18 degrees C for a minimum of 24 h,  and treat equipment (and window
perimeters) with Kelthane AP (dicofol;
1,1-bis(p-chlorophenyl)2,2,2-trichloroethanol).

Best of luck.

-----------------------------------------------------------
Matthew Sachs
Department of Chemistry, Biochemistry and Molecular Biology
Oregon Graduate Institute of Science and Technology
20000 NW Walker Road
P.O. Box 91000
Portland, OR  97291-1000
503 690-1487 Phone
503 690-1464 Fax
[email-masked]