
>From LISS@alf1.ngate.uni-regensburg.de Wed Jun 23 10:40:10 1993
To: archive@bio.indiana.edu
>From: "Michael Liss"  <LISS@alf1.ngate.uni-regensburg.de>
Subject:      i had problems via ftp, try this way now
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                              INTRON  ANALYZER
                                Version  3.0

                                 Written by
                                Michael Liss
                          University of Regensburg
                                  Germany
                                 (c)  1992



Inspired by  an article  of Witold Filipowicz (Friedrich Miescher-Institut,
CH-4002  Basel,   Switzerland),  which   documents  that  there  are  basic
differences in  the base composition of introns from animals and plants, we
wanted to  examine the introns of Volvox carteri, a multi-cellular alga, to
find regularities in them. And this is exactly what this program does. From
a given list of at most 350 introns you can explore them by ligning them up
either at the 5' or at the 3'end or you may study the adjoining exon-parts.
The program will  then build  a consensus-sequence which shows what base or
type of base (Pur/Pyr,  AT/GC) is  most frequent  in each  position. Also a
graphic plot is possible, where these frequencies are displayed in percent.


System requirements

INTANA needs  an IBM compatible machine  with  MS- or PC-DOS 3.0  or later.
The connected monitor can be color or b/w. An EGA card is suggested to view
graphics. The  standard configuration  supports an  Epson compatible matrix
printer, although  any other  printer can  be  controled  by  changing  the
INTANA.CFG file.


Files after unziping INTANA.ZIP:

 - INTANA.EXE   : The INTron ANAlyzing program
 - INTANA.CFG   : The configuration file used by INTANA.EXE
 - INTANA.DOC   : Documentation
 - INTCUT.EXE   : The INTron CUTting program used to extract 
                  the introns from given sequence files

   Sample files to test INTANA:

 - NRED.SEQ     : Sequence of Nitrate-reductase from  Volvox carteri
 - UBI.SEQ      : Sequence of Ubiquitin from Volvox carteri
 - VOLVOX.CUT   : Index file used by INTCUT.EXE 
                  (containing "NRED.SEQ" & "UBI.SEQ")




