                             PROANAL ver 2.0
                             SUBMISSION FORM

Present title and address:Leading scientist, Principal investigator
                          Theoretical Department
                          Research Institute of Molecular  Biology,
                          State Recearch Center of Virology and
                          Biotechnology "Vector",
                          Koltsovo, Novosibirsk Region
                          633159  Russia
                          Tel: +7 (3832) - 64 7774 work
                               +7 (3832) - 64 7616 home
                          Telex: 133196 NPO SU,
                          Fax: +7 (3832) - 328831
                          E.mail: eroshkin@vector.nsk.su

Program description is  published in:

Eroshkin A.M., Fomin V.I., Zhilkin P.A.
 Algorithm and computer program ProAnal for analysis of
 relationship between structure and activity in a family of proteins or
 peptides.
CABIOS,  vol 9, 491-497, 1993

Short description:

     Analysis of multiple protein alignments, studying the
structure-function and structure-activity relationships in
protein/peptide families, enhanced version of program PROANAL
for IBM PC/AT or clone. New options: convert alignments from
CLUSTAL, plots individual and average profiles, seeks physico-
chemically variable and conservative regions, sites with high
and low physico-chemical properties, has large set of aa
properties, complete manual, help.

Operating system:  MS-DOS

Hardware requirements: EGA/VGA card

Copyright status:  Copyrighted, but free (Freeware)
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                     Pro_Anal

      PROTEIN STRUCTURE-ACTIVITY ANALYZING SYSTEM

                VERSION 1.0 Beta
                     1993
                distributed freely

   Pro_Anal was written for the analysis of relationships between structure
and  activity  in  a family  of  evolutionary related  (or/and
artificially mutated) proteins/peptides.

   The program uses  aligned  amino  acid  sequences with  data on  their
activity (pK,  Km,  ED50 or any other) and searches for  correlations
between  activity  data  and  various physical-chemical characteristics of
regions in the primary structure.  In automatical mode the program generates
and verifies hypotheses on  the  location of a sequential modulating
region in a protein and key characteristics of this region.  In manual
mode users can generate and analyze their own hypotheses.  In addition,
the program looks for protein conservative domains (functional sites) and
variable regions (sites for  engineering).

   This program is the enhanced version of the program described in:
--------------------------------------------------------------------
   Eroshkin A.M., Zhilkin P.A., Fomin V.I.  Algorithm   and computer
program  Pro_Anal  for  analysis  of relationship between structure and
activity in  a  family of proteins or peptides.  CABIOS, 1993, v.9, n.5,
491-497.
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   The improvements are the following:
- it is not necessary to input the thresholds for correlation
coefficients in "Automatical Mode";
- in addition to  the search of  modulating  centers it is possible
to find regions conservative (or variable) in changes of average
properties, alpha-helical and beta-strand moments and other
characteristics.
   Program is implemented for IBM PC or compatible computers.  It is
designed to be easily handled by any occasional computer user and yet it
is powerful enough for experienced professionals. This version of the
program is distributed freely.

The distribution diskette contains the following files:

READ.ME       |  This file
PRO_ANAL.EXE  |  Executing modules
BROWSE.COM    |
BROWSE.COM    |
BROW.EXE      |
EGAVGA.BGI    |
HELP.TXT      |
MANUAL.TXT    |  Program description
ARTICLE       |
KIDERA.PPT    |  Amino acid physico-chemical properties
PROPERTY.PPT  |
DISINT.ALI    |  Data of examples (two sets)
DISINT.ACT    |
DISINT.SEQ    |
IMMUNOGE.ALI  |
IMMUNOGE.ACT  |
IMMUNOGE.SEQ  |

    All listed files should be in the same directory.  There are two
examples on the distribution disk:
DISINT (files DISINT.SEQ, DISINT.ACT, DISINT.ALI) - family of
disintegrins with studied activity in test of inhibition of platelet
aggregation;
IMMUNOGE (files IMMUNOGE.SEQ, IMMUNOGE.ACT, IMMUNOGE.ALI)  - a number  of
unrelated peptides  with  tested immunogenicity.

Authors address:

Eroshkin  Alexey                  EROSHKIN@VECTOR.NSK.SU
Research Institute  of            Tel.+7-(3832)-64-77-74
Molecular  Biology,               Fax:+7-(3832)-32-88-31
NPO "Vector", Koltsovo,           Telex 133196 NPOSU
Novosibirsk region, 633159,
Russia.

Peter Zhilkin                     zhilkin@zeno.ibd.nrc.ca
IBD NRC                           Tel: +1-204-984-4540
435 Ellice Ave. Winnipeg          Fax: +1-204-984-5472
Manitoba R3B 1Y6 Canada
