110469 Regression Analysis for Michaelis-Menten Kinetic Data Version: January 1981 Submitted by: G. C. Scott Operating System: RT-11 Source Language: FORTRAN IV Memory Required: 30K Vir.Mem.,2K Dir.Address.Mem. for array storage Keywords: Scientific Applications, Statistics Abstract: This FORTRAN program calculates the Michaelis-Menton parameters Vm, the maximum velocity, and Km, the substrate concentration at half-maximal velocity, and their 95% confidence ranges for the dependence of enzyme activity of substrate concentration, in the absence of product. The program uses simple non-linear regression, non-parametric statistics, unweighted linear regression, and iterative weighted linear regression. The program requests the following inputs from the user: a selection of the type(s) of regression(s) to be performed, a file of substrate and rate data containing up to 50 data points, and an appropriate T-statistic. The initial Km bracket values for the non-linear calculations are assumed to be BRKM(1) = 0.0 and BRKM(2) = 10.0. If this interval does not bracket the true Km, BRKM(2) is doubled until the appropriate bracket interval is obtained. Any negative Vm or Km values generated by the non-parametric calculations are replaced by an arbitrarily large number (1.0E+6) and entered into the sorting routine with the other smaller and non-negative Vm and Km values. The final two values of the iterative weighted linear sum of least squares calculations are tested for a convergence tolerance of 5.0%. Restrictions: Regression cannot be performed for more than 50 data pairs. Media (Service Charge Code): Source Listing (BA), One RX01 Diskette (KA) Format: RT-11, 600' Magnetic Tape (MA) Format: RT-11

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