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Class Number: 935
Class Title: GENETIC ENGINEERING: RECOMBINANT DNA TECHNOLOGY, HYBRID OR
FUSED CELL TECHNOLOGY, AND RELATED MANIPULATIONS OF NUCLEIC
ACIDS
Subclass Subclass
Number Title
1 OBTAINING THE DESIRED GENE; DNA, RNA PER SE AND THE MODIFICATION
THEREOF OTHER THAN VECTOR MODIFICATION
2 .DNA - RNA hybrid
3 .RNA
4 ..mRNA
5 ..2-100 nucleotides in length, e.g., t-RNA, etc.
6 .DNA, e.g., regulatory sequences, etc.
7 ..Homopolymeric, e.g., poly d(A) sequence, etc.
8 ..12-75 nucleotides in length, e.g., primers, etc.
9 ..Structural gene sequence
10 ...Modified structural gene, e.g., nonnaturally occuring
sequence, etc.
11 ...Polypeptide
12 ....Antigenic material
13 ....Hormone, e.g., human growth factor, insulin, etc.
14 ....Enzyme
15 ....Antibody
16 .Methods of producing DNA or RNA other than by expression
vectors, e.g., culture of cells high in DNA, etc.
17 ..Cell free production
18 ...cDNA synthesis
19 .Isolation or purification of DNA or RNA
20 ..RNA
21 ...mRNA
22 VECTORS AND METHODS OF MODIFYING VECTORS
23 .Inserting a gene into a vector to form a recombinant vector,
i.e., cleavage and ligation
24 ..Vector utilized, e.g., episomes, etc.
25 ...Plant virus
26 ...Cosmid
27 ...Plasmid
28 ....Yeast
29 ....Prokaryotic
30 ....Plant
31 ...Bacteriophage
32 ...Animal virus, e.g., SV40, etc.
33 METHODS OF ENHANCING OR DIMINISHING EXPRESSION
34 .Eukaryotic cell
35 ..Plant cell
36 ..Transcription
37 ..Yeast cell
38 .Prokaryotic cell
39 ..Transcription
40 ...Operon selection
41 ....Promoter, e.g., portable promoters, etc.
42 ..Gene dosage modification, e.g., copy number amplification,
etc.
43 ...Inducible, e.g., temperature inducible, etc
44 ..Translation
45 ...Ribosome binding sitee
46 ...Initiation
47 .Fused protein or peptide
48 ..Signal peptide, e.g., secretion, etc.
49 .Post translational modification
50 ..Glycosylation
51 ..Peptide bond cleavage
52 METHODS OF INTRODUCING A GENE INTO A HOST CELL, E.G.,
TRANSFORMATION OR TRANSFECTION, ETC.
53 .Microinjection
54 .Microencapsulation, e.g., liposome vesicle etc.
55 .Using vector, e.g., plasmid, etc.
56 ..Plasmid
57 ..Virus
58 ...Phage, e.g., phage lambda, etc.
59 METHOD OF USE OF GENETICALLY ENGINEERED CELLS, E.G., OIL SPILL
CLEANUP, ETC.
60 .To produce an identified chemical product e.g., amino acid,
etc.
61 ..Yield optimization
62 .Control of genetic diseases or defects by use of added gene
63 .Use im animal husbandry
64 .Use in agriculature
65 .Vaccine production
66 CELLS CONTAINING A VECTOR AND/OR EXOGENOUS GENE PER SE;
PROPAGATION THEREOF; OTHER MEMBRANE ENCAPSULATED DNA, E.G.,
PROTOPLASTS, ETC.
67 .Plant cells
68 .Fungal cells
69 ..Yeast cells
70 .Animal cell
71 ..Human cell
72 .Bacteria
73 ..Escherichia
74 ..Bacillus
75 ..Streptomyces
76 ASSAY RELATED TO GENETIC ENGINEERING
77 .Methods of analysis of nucleic acids
78 ..Including hybridization
79 .Methods of selection of recombinant gene containing vector;
materials therefore, e.g., replica plating, etc.
80 ..Gene library manipulation
81 ..Antigen-antibody
82 ..Enzyme activity
83 ..Host suicide
84 ..Selection medium
85 GENETIC ENGINEERING APPARATUS
86 .Analytical, e.g., for autoradiography, etc.
87 ..Automated
88 .Synthesis, e.g., peptide or gene synthesizers, etc.
89 HYBRID OR FUSED CELL TECHNOLOGY, E.G., HYBRIDOMA, ETC.
90 .Method of selection of the desired cell
91 ..Of plant cells, e.g., protoplasts, etc.
92 ..Using positive selection technique
93 .Method of production of hybrid or fused cells, e.g., chromosome
or genome transfer techniques, etc.
94 ..Of plant cells
95 .Fused or hybrid cell per se
96 ..Interspecies fusionn
97 ..Fungi, e.g., yeasts, etc.
98 ..Plant cells
99 ..Human cell
100 ...B lymphocyte
101 ...T lymphocyte
102 ..Animal cell
103 ...Murine cell, e.g., mouse cell, etc.
104 ....B lymphocyte
105 ....T lymphocyte
106 .Method of use of the fused or hybrid cell or the product
thereof
107 ..In vivo use of product
108 ..In vitro, e.g., cell cultivation techniques, affinity
chromatography, etc.
109 ...Production of non-antibody product
110 ...For use as testing material
111 MISCELLANEOUS