Re: [compost_tea] Re: New Subject Crown Gall

From: <soilfoodweb_at_aol.com>
Date: Thu, 09 Oct 2003 08:41:10 -0400

Hi Laura -

I have worked with the person who isolated and tested the bacterium you are interested in. Yes, it should grow in compost tea, and it likes amino sugars. So, reasonably complex sugars should work. A little testing with an SFI lab would tell you which food resource should work best.

For example, bring a tea to Paul at the New York lab. Look at the organisms in the tea (takes about 5 minutes to prep, about 20 minutes to look at the sample to see what's present). Add in the bacterial inoculum, find out what the bacterium looks like. Split the tea into a number of smaller portions, depending on how many foods you want to test. Place a small amount (like one crop of food in a 500 ml container) of the different foods in the containers with the tea and Agrobacterium. Let the samples incubate while you go eat lunch, come back and look at the samples to see whether the Agrobacterium started to grow. Let incubate overnight, and check again.

That way you know what food will feed the organisms. You might want to then test concentration of the food to add - for example, add 2 drops, or 4 drops, or 8 drops of the best food, so you get a better idea of what increasing foods do. When the brew runs out of air overnight, then the organisms won't grow, and you know you added too much food. Or get an oxygen probe and test that way.

OK?

Elaine Ingham
President, Soil Foodweb Inc.
SFI Corvallis, OR
SFI Port Jefferson, NY
SFI Lismore, NSW, Australia
SFI Hilversum, The Netherlands
SFI Cambridge, New Zealand
www.soilfoodweb.com

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Received on Thu Oct 09 2003 - 21:34:57 EDT

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