Dissertation Information for Michael BecichNAME:
DEGREE:
DISCIPLINE:
SCHOOL: ADVISORS: COMMITTEE MEMBERS: MPACT Status: Incomplete - Inspected Title: TUMOR HETEROGENEITY IN THE TRANSPLANTABLE PANCREATIC CARCINOMA OF RAT: ISOLATION OF DISTINCT NEOPLASTIC SUBPOPULATIONS TO STUDY CYTODIFFERENTIATION
Abstract: "The transplantable pancreatic acinar carcinoma demonstrates heterogeneity of cytodifferentiation with cell types ranging from those containing abundant, well-developed zymogen granules to those with a virtual absence of secretory granules. This spectrum of cytodifferentiation mimics that of normal embryonic acinar cell development in the pancreas. Two relatively homogeneous subpopulations of cells were isolated from this neoplasm by isopyknic gradient centrifugation. One subpopulation, termed granule enriched fraction (GEF) contained morphologically differentiated cells with abundant mature zymogen granules. The other subpopulation, consisted of poorly differentiated cells with immature cytodifferentiation and was termed granule deficient fraction (GDF) cells. The isolation of these tumor cell subpopulations was central to developing a model to study tumor heterogeneity without necessitating prolonged in vitro passage of subcloned cells which can alter surface properties and morphogenetic programming in epithelial cells. |
MPACT Scores for Michael BecichA = 0 Advisors and Advisees Graphgenerating graph, please reload |
Students under Michael Becich
ADVISEES:
- None
COMMITTEESHIPS:
- Sujin Kim - University of Pittsburgh (2003)